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The map label for this gene is alaS [H]

Identifier: 226949919

GI number: 226949919

Start: 2874600

End: 2877239

Strand: Reverse

Name: alaS [H]

Synonym: CLM_2871

Alternate gene names: 226949919

Gene position: 2877239-2874600 (Counterclockwise)

Preceding gene: 226949920

Following gene: 226949918

Centisome position: 69.24

GC content: 30.49

Gene sequence:

>2640_bases
ATGGAAAGAATGGGTTTAAATGAAATAAGAGAAGAATATCTAAAGTTTTTTGAAAGTAAAGCACATTTAAGATTGCCGAG
CTTTTCTTTAGTGCCTAAAAATGATAAAAGTTTACTTTTAATAAATGCAGGAATGGCCCCTTTAAAGCCTTATTTTACAG
GGCTTCAAGTTCCACCAAATAAAAGGGTCACTACTTGTCAAAAATGTGTAAGAACTGGAGATATTGAAAATGTAGGTAAA
ACTTCAAGGCATGGTACTTTCTTTGAAATGATGGGAAATTTTTCCTTCGGGGATTATTTTAAAGAAGAAGTTATTCCTTG
GGCTTGGGAATTTACTACAGAGGTTTTAAAATTACCTAAAAACAAATTGTATGTGACTATATATGAGGATGATGATGAAG
CTTTAGACATATGGGTAAATAAAACAGATGTGGATCCTAAAAGAATATTTAGATTAGGAAAAGAAGATAATTTTTGGGAA
CATGGATTAGGACCTTGTGGACCTTGTTCAGAAATTCACTTTGATAGGGGAGCAGGAGAGGTTAAAACTTCAGAGGAATT
TGTAAAAGCTAGTGATGAGGATAAAATAGTAGAATTTTGGAACTTAGTATTTACTCAATTTGATAAAGATGAAGAAGGAA
ATTATAATAAATTAGCTAATCCTAATATAGATACAGGTATGGGGCTTGAAAGAATGGCTACTATAATGCAAAATGTGGAT
ACTATATTTGAAGTGGATACAATAAGAGCTGTATTAGATAAGGTGTGTAAACTTTCAGGAGCTAATTACAAAGAAGATAG
AGTAAAAGATATATCTATAAGAATAATAACAGATCACATAAGAAGTATTACTTTTATGATAAGCGATGGTATATTACCTT
CTAATGAAGGAAGAGGATATGTTCTTAGAAGACTTTTAAGAAGAGCTGCAAGACATGGAAAAACTTTAGGAATAAACAAT
ACATTTCTACATAACTTAACAGACATAGTTATAGAAAATTGCTATAAAAATTATCCGGAATTAGAAGAAAAAAGAGAATA
TATAAAGAAAATTATAAAATTAGAAGAAGAAAGATTTGATGAAACTATAGATGCTGGAATGCAAATATTAAATGATTATA
TAAAAGAAGTTAAGAATAATAATTATAAGATCCTTTCAGGAGATAAAGCTTTCAAACTATATGATACTTATGGATTCCCA
GTAGAATTAACAGAAGAAATATTAGAAGAGGAAGGTATAAGTATAGATAAAGAAGGTTTCAACAAAGAAATGAAGGAGCA
AAGAGAAAGAGCTAGATCCGCTAGAGAAGAAACTAATTATATGGGGGCAGAGGATACTATATTAAATAAAATAGATCTAA
ATATAAATACAGACTTTGAAGGTTACGATAAACTAGAAGTAAAATCAAAAGTGGCTGTGATTATAAAAGATGAAGAGTTC
AAAAATGAAATGGAAAAAGGGAATGAAGGGATAATAGTAACTTATAATACTCCATTTTATGCAGAAATGGGTGGTCAAAC
AGGAGATACAGGTATTATATATAACGATAATTTTAAAGCAGAGGTTATAGATTGTAAAAAGAACATATCAGGTAAAATAC
TTCATTTTGTAAAAATTTTAGATGGAAAAGTTGCTTTAGAAGATCAAGTTATATTAAAAGTAAATGAAGAAAGAAGAAAT
AATATAAGAAAAAATCATACAGCAACCCATATACTACATGCTGCTTTAATAAAAGTGGTAGGAGATCATGTACAACAATC
AGGTTCTTATGTGGATGATGAAAGACTAAGATTTGACTTTAGTCATTTTGAAGCAGTATCAGAGGATGAATTAAAAGAAG
TAGAGAAGATAGTAAATAAAGAGATAATGAAAGCCAATGCTGTAAATACTAAAGTAATGAATATAGAAGAAGCAAAACAG
CAGGGGGCTATTGCATTATTCGATAATAAATATAAGGATGATGTTAGAGTAGTTTCTGTAGGAGATTTTAGTAAAGAATT
ATGTGGTGGAACTCATGTAAGTAATAGTGGCCAGATTGGAATGTTTAAAATAGTTTCCGAGGCAGGGGTTGCAGCTGGCA
TAAGAAGAATAGAGGCAGTAACGGCTTTTAAAGCTATGGAATATGTAGATCATAAAAATAACATATTAAAAGAAGCAGCT
CAAATATTAAAATGTAACGAAAAGGAATTATTAAATAAATTAAATCATCAAGTTCTAGAAATGAAAGAAAAGGAAAAGGA
AATAGAAGCTTTAAAATTAAAATTAGCAAGTGGTGCAGAAGATGAAATATTAGATAACATAAAAGAAATAAAAGGTGTCA
AAGTAGCATCAGCTGCAGTAAAGGATATAGATGGGAATGCCCTAAGAGATTTAGGCGATAAAATAAGAGATAATATGCAA
AGTGGAGTAGTTGTTCTAGGAAGTAACTATAAAGGTAAAGTTTTATTTGTTGCTATGGCAACTAAGGATACAGTGGCTAA
AGGAGTGCATTGTGGAAAAATAATTAAAGAGGTTGCAACTATAGCCGGTGGTGGTGGTGGTGGAAGACCAGACATGGCAC
AGGCAGGAGGTAAAGATCCAAATAAATTAGAAGATGCTATAAAAACAGTAGAAACTGTAGTTGAAAGTTTAGTTAAATAG

Upstream 100 bases:

>100_bases
ATTTAAATTAGGGTGGTACCGCGGAATATAACTTTCGTCCCTTTTAGGGATTGAAGGTTTTTTTTATTATAAAAATATGA
GAATAAAGGAGAGATAAACC

Downstream 100 bases:

>100_bases
TATACTTTTTAATAAATATGTCTTATAATATAATTAAAGAACATAAAAAACACTCAATACTGGTTGCTCTAAAGAAAGTT
GCAATCCTTAAAGATTGTAT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 879; Mature: 879

Protein sequence:

>879_residues
MERMGLNEIREEYLKFFESKAHLRLPSFSLVPKNDKSLLLINAGMAPLKPYFTGLQVPPNKRVTTCQKCVRTGDIENVGK
TSRHGTFFEMMGNFSFGDYFKEEVIPWAWEFTTEVLKLPKNKLYVTIYEDDDEALDIWVNKTDVDPKRIFRLGKEDNFWE
HGLGPCGPCSEIHFDRGAGEVKTSEEFVKASDEDKIVEFWNLVFTQFDKDEEGNYNKLANPNIDTGMGLERMATIMQNVD
TIFEVDTIRAVLDKVCKLSGANYKEDRVKDISIRIITDHIRSITFMISDGILPSNEGRGYVLRRLLRRAARHGKTLGINN
TFLHNLTDIVIENCYKNYPELEEKREYIKKIIKLEEERFDETIDAGMQILNDYIKEVKNNNYKILSGDKAFKLYDTYGFP
VELTEEILEEEGISIDKEGFNKEMKEQRERARSAREETNYMGAEDTILNKIDLNINTDFEGYDKLEVKSKVAVIIKDEEF
KNEMEKGNEGIIVTYNTPFYAEMGGQTGDTGIIYNDNFKAEVIDCKKNISGKILHFVKILDGKVALEDQVILKVNEERRN
NIRKNHTATHILHAALIKVVGDHVQQSGSYVDDERLRFDFSHFEAVSEDELKEVEKIVNKEIMKANAVNTKVMNIEEAKQ
QGAIALFDNKYKDDVRVVSVGDFSKELCGGTHVSNSGQIGMFKIVSEAGVAAGIRRIEAVTAFKAMEYVDHKNNILKEAA
QILKCNEKELLNKLNHQVLEMKEKEKEIEALKLKLASGAEDEILDNIKEIKGVKVASAAVKDIDGNALRDLGDKIRDNMQ
SGVVVLGSNYKGKVLFVAMATKDTVAKGVHCGKIIKEVATIAGGGGGGRPDMAQAGGKDPNKLEDAIKTVETVVESLVK

Sequences:

>Translated_879_residues
MERMGLNEIREEYLKFFESKAHLRLPSFSLVPKNDKSLLLINAGMAPLKPYFTGLQVPPNKRVTTCQKCVRTGDIENVGK
TSRHGTFFEMMGNFSFGDYFKEEVIPWAWEFTTEVLKLPKNKLYVTIYEDDDEALDIWVNKTDVDPKRIFRLGKEDNFWE
HGLGPCGPCSEIHFDRGAGEVKTSEEFVKASDEDKIVEFWNLVFTQFDKDEEGNYNKLANPNIDTGMGLERMATIMQNVD
TIFEVDTIRAVLDKVCKLSGANYKEDRVKDISIRIITDHIRSITFMISDGILPSNEGRGYVLRRLLRRAARHGKTLGINN
TFLHNLTDIVIENCYKNYPELEEKREYIKKIIKLEEERFDETIDAGMQILNDYIKEVKNNNYKILSGDKAFKLYDTYGFP
VELTEEILEEEGISIDKEGFNKEMKEQRERARSAREETNYMGAEDTILNKIDLNINTDFEGYDKLEVKSKVAVIIKDEEF
KNEMEKGNEGIIVTYNTPFYAEMGGQTGDTGIIYNDNFKAEVIDCKKNISGKILHFVKILDGKVALEDQVILKVNEERRN
NIRKNHTATHILHAALIKVVGDHVQQSGSYVDDERLRFDFSHFEAVSEDELKEVEKIVNKEIMKANAVNTKVMNIEEAKQ
QGAIALFDNKYKDDVRVVSVGDFSKELCGGTHVSNSGQIGMFKIVSEAGVAAGIRRIEAVTAFKAMEYVDHKNNILKEAA
QILKCNEKELLNKLNHQVLEMKEKEKEIEALKLKLASGAEDEILDNIKEIKGVKVASAAVKDIDGNALRDLGDKIRDNMQ
SGVVVLGSNYKGKVLFVAMATKDTVAKGVHCGKIIKEVATIAGGGGGGRPDMAQAGGKDPNKLEDAIKTVETVVESLVK
>Mature_879_residues
MERMGLNEIREEYLKFFESKAHLRLPSFSLVPKNDKSLLLINAGMAPLKPYFTGLQVPPNKRVTTCQKCVRTGDIENVGK
TSRHGTFFEMMGNFSFGDYFKEEVIPWAWEFTTEVLKLPKNKLYVTIYEDDDEALDIWVNKTDVDPKRIFRLGKEDNFWE
HGLGPCGPCSEIHFDRGAGEVKTSEEFVKASDEDKIVEFWNLVFTQFDKDEEGNYNKLANPNIDTGMGLERMATIMQNVD
TIFEVDTIRAVLDKVCKLSGANYKEDRVKDISIRIITDHIRSITFMISDGILPSNEGRGYVLRRLLRRAARHGKTLGINN
TFLHNLTDIVIENCYKNYPELEEKREYIKKIIKLEEERFDETIDAGMQILNDYIKEVKNNNYKILSGDKAFKLYDTYGFP
VELTEEILEEEGISIDKEGFNKEMKEQRERARSAREETNYMGAEDTILNKIDLNINTDFEGYDKLEVKSKVAVIIKDEEF
KNEMEKGNEGIIVTYNTPFYAEMGGQTGDTGIIYNDNFKAEVIDCKKNISGKILHFVKILDGKVALEDQVILKVNEERRN
NIRKNHTATHILHAALIKVVGDHVQQSGSYVDDERLRFDFSHFEAVSEDELKEVEKIVNKEIMKANAVNTKVMNIEEAKQ
QGAIALFDNKYKDDVRVVSVGDFSKELCGGTHVSNSGQIGMFKIVSEAGVAAGIRRIEAVTAFKAMEYVDHKNNILKEAA
QILKCNEKELLNKLNHQVLEMKEKEKEIEALKLKLASGAEDEILDNIKEIKGVKVASAAVKDIDGNALRDLGDKIRDNMQ
SGVVVLGSNYKGKVLFVAMATKDTVAKGVHCGKIIKEVATIAGGGGGGRPDMAQAGGKDPNKLEDAIKTVETVVESLVK

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=976, Percent_Identity=33.5040983606557, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI38569417, Length=768, Percent_Identity=29.4270833333333, Blast_Score=359, Evalue=7e-99,
Organism=Escherichia coli, GI1789048, Length=870, Percent_Identity=42.7586206896552, Blast_Score=719, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=985, Percent_Identity=33.8071065989848, Blast_Score=481, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17536681, Length=750, Percent_Identity=33.2, Blast_Score=366, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324911, Length=945, Percent_Identity=35.8730158730159, Blast_Score=500, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24582809, Length=970, Percent_Identity=32.5773195876289, Blast_Score=481, Evalue=1e-135,
Organism=Drosophila melanogaster, GI45552267, Length=970, Percent_Identity=32.5773195876289, Blast_Score=481, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24658214, Length=779, Percent_Identity=28.2413350449294, Blast_Score=289, Evalue=6e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 99331; Mature: 99331

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERMGLNEIREEYLKFFESKAHLRLPSFSLVPKNDKSLLLINAGMAPLKPYFTGLQVPPN
CCCCCHHHHHHHHHHHHHCCCCEECCCEEECCCCCCEEEEEECCCCCCCHHHCCCCCCCC
KRVTTCQKCVRTGDIENVGKTSRHGTFFEMMGNFSFGDYFKEEVIPWAWEFTTEVLKLPK
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCC
NKLYVTIYEDDDEALDIWVNKTDVDPKRIFRLGKEDNFWEHGLGPCGPCSEIHFDRGAGE
CCEEEEEEECCCCEEEEEEECCCCCHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCC
VKTSEEFVKASDEDKIVEFWNLVFTQFDKDEEGNYNKLANPNIDTGMGLERMATIMQNVD
CCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
TIFEVDTIRAVLDKVCKLSGANYKEDRVKDISIRIITDHIRSITFMISDGILPSNEGRGY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHHHEEEEEECCCCCCCCCCHH
VLRRLLRRAARHGKTLGINNTFLHNLTDIVIENCYKNYPELEEKREYIKKIIKLEEERFD
HHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ETIDAGMQILNDYIKEVKNNNYKILSGDKAFKLYDTYGFPVELTEEILEEEGISIDKEGF
HHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHC
NKEMKEQRERARSAREETNYMGAEDTILNKIDLNINTDFEGYDKLEVKSKVAVIIKDEEF
CHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEEEEECCCCCCCCCEEECCEEEEEEECHHH
KNEMEKGNEGIIVTYNTPFYAEMGGQTGDTGIIYNDNFKAEVIDCKKNISGKILHFVKIL
HHHHHCCCCCEEEEECCCEEEECCCCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHH
DGKVALEDQVILKVNEERRNNIRKNHTATHILHAALIKVVGDHVQQSGSYVDDERLRFDF
CCCEEECCEEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEH
SHFEAVSEDELKEVEKIVNKEIMKANAVNTKVMNIEEAKQQGAIALFDNKYKDDVRVVSV
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHCCCEEEECCCCCCCEEEEEE
GDFSKELCGGTHVSNSGQIGMFKIVSEAGVAAGIRRIEAVTAFKAMEYVDHKNNILKEAA
CCHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QILKCNEKELLNKLNHQVLEMKEKEKEIEALKLKLASGAEDEILDNIKEIKGVKVASAAV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHH
KDIDGNALRDLGDKIRDNMQSGVVVLGSNYKGKVLFVAMATKDTVAKGVHCGKIIKEVAT
HCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEECHHHHHHCCHHHHHHHHHHH
IAGGGGGGRPDMAQAGGKDPNKLEDAIKTVETVVESLVK
HCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERMGLNEIREEYLKFFESKAHLRLPSFSLVPKNDKSLLLINAGMAPLKPYFTGLQVPPN
CCCCCHHHHHHHHHHHHHCCCCEECCCEEECCCCCCEEEEEECCCCCCCHHHCCCCCCCC
KRVTTCQKCVRTGDIENVGKTSRHGTFFEMMGNFSFGDYFKEEVIPWAWEFTTEVLKLPK
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCC
NKLYVTIYEDDDEALDIWVNKTDVDPKRIFRLGKEDNFWEHGLGPCGPCSEIHFDRGAGE
CCEEEEEEECCCCEEEEEEECCCCCHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCC
VKTSEEFVKASDEDKIVEFWNLVFTQFDKDEEGNYNKLANPNIDTGMGLERMATIMQNVD
CCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
TIFEVDTIRAVLDKVCKLSGANYKEDRVKDISIRIITDHIRSITFMISDGILPSNEGRGY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHHHEEEEEECCCCCCCCCCHH
VLRRLLRRAARHGKTLGINNTFLHNLTDIVIENCYKNYPELEEKREYIKKIIKLEEERFD
HHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ETIDAGMQILNDYIKEVKNNNYKILSGDKAFKLYDTYGFPVELTEEILEEEGISIDKEGF
HHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHC
NKEMKEQRERARSAREETNYMGAEDTILNKIDLNINTDFEGYDKLEVKSKVAVIIKDEEF
CHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEEEEECCCCCCCCCEEECCEEEEEEECHHH
KNEMEKGNEGIIVTYNTPFYAEMGGQTGDTGIIYNDNFKAEVIDCKKNISGKILHFVKIL
HHHHHCCCCCEEEEECCCEEEECCCCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHH
DGKVALEDQVILKVNEERRNNIRKNHTATHILHAALIKVVGDHVQQSGSYVDDERLRFDF
CCCEEECCEEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEH
SHFEAVSEDELKEVEKIVNKEIMKANAVNTKVMNIEEAKQQGAIALFDNKYKDDVRVVSV
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHCCCEEEECCCCCCCEEEEEE
GDFSKELCGGTHVSNSGQIGMFKIVSEAGVAAGIRRIEAVTAFKAMEYVDHKNNILKEAA
CCHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QILKCNEKELLNKLNHQVLEMKEKEKEIEALKLKLASGAEDEILDNIKEIKGVKVASAAV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHH
KDIDGNALRDLGDKIRDNMQSGVVVLGSNYKGKVLFVAMATKDTVAKGVHCGKIIKEVAT
HCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEECHHHHHHCCHHHHHHHHHHH
IAGGGGGGRPDMAQAGGKDPNKLEDAIKTVETVVESLVK
HCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA