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The map label for this gene is infB [H]

Identifier: 226949771

GI number: 226949771

Start: 2723003

End: 2725069

Strand: Reverse

Name: infB [H]

Synonym: CLM_2711

Alternate gene names: 226949771

Gene position: 2725069-2723003 (Counterclockwise)

Preceding gene: 226949772

Following gene: 226949770

Centisome position: 65.58

GC content: 31.88

Gene sequence:

>2067_bases
TTGGCAAAAATAAGAGTATATGAATTAGCTAAAGAATTAAACATATCAAGTAAAGAACTTATAACATTATTAGAAGAAGA
ATTTAGTGTAGAAGTTAAAAACCATATGAGCGCTATAGAAGATGAGGATGCAAATCTTATAAAAGAACTATTATCAGGTA
AAGAAAAGTCAGAAAAAACAAAGGAAGAAGATGATGAAATAGAAACTACAGCGAAAAACCCAATAAAAGAATCAATGAAT
AATAAAAAATCTAATAAAAGAGATGACAAAAATGAAAAAGTAAATACTGAAAATGCAGAAGATATGGGTATAATAACAAT
GACTTCTGATACTATTACAGTAAAAGAAATATCAGATAAATTGGAAAAATCCTATGCAGAAGTTATAAAAGAACTAATGC
TTATGGGTGTTATGGCTTCTGTGAATCAAGAGATAAACTTTGAAATGGCTGAAAAATTAGCAGCAAAATTTGATATGGAA
ATTCTAAAAGAAGATGAAGATGAGAAAGAGGATCTAGAAGATATATTAAAAGATAATGAAGAAGAAGAATATTTACAAAA
AAGATCCCCTATCATAACTGTTATGGGTCACGTTGACCACGGAAAAACTTCTTTATTAGATGCTATAAGAAAATCAAAGA
TAACTTCTACAGAGGCAGGAGGAATAACCCAACATATAGGTGCTTATACTGTGGAATTAAATGGAGAAGCTATAACTTTC
TTAGATACTCCAGGCCACGCAGCTTTTACAGCTATGAGAGCAAGAGGAGCGCAAGTTACAGATATAGTTATATTAGTAGT
AGCAGCAGATGACGGAATTATGCCTCAAACTCAAGAAGCTATAAGTCACTGTAAGGCAGCTAATGTTCCATTAATAGTTG
CTATAAATAAAATAGATAGACCTGGTGCTAATATAGATAAAGTAAAACAAGAATTAACAGAATATGGATTAGTAGCAGAG
GACTGGGGTGGAGATACTATATGTGTTCCAGTTTCAGCTCATACAAAAGAAGGTATAGATGATCTACTAGAAATGATACT
TTTATCATCAGAAATACTTGAATTGAAAGCAAACCCAAATAGAAAAGCAAAGGGAACAGTAGTAGAAGCTAAACTTGATA
AAGGAAGAGGACCAGTAGCTACATTACTTATTCAAAATGGTACTTTAAGAGTAGGAGATTCTATAGTAGTGGGATCTACA
TATGGAAGAATAAGAGCTATGTTTAATGATAAAGGTAGAAATATAGAATCAGCAGGCCCTTCTACACCAGTAGAAATACT
AGGTTTATCAGAAGTTCCTGAAGCAGGAGATAAATTCTACCAAGTTAAAGAGGAAAAAACTGCCAGAGGTATTGCAGATA
AGAGAAAAGAAAAAATAAGAGATGAATATCTACAATCTACCCATAAAGTTTCTCTAGAAGATTTATACAATCAAATACAA
GAAGGAACAGTAAAGGAATTAGGATTAATTGTAAAAGCTGATGTTCAGGGTTCTGTAGAGGCTTTAAAACAATCCTTAGA
AAAGCTTTCAACAGAAGAAGTAAAGGTTAGAGTTATTCATGGAGGTGTTGGAGCTATAAATGAAACAGATGTTACATTAG
CAACAGCTTCTAATGGTATTATATTAGGCTTTAATGTAAGACCGGATAACAATGCTATTATAGCTTCTGAAAGAGACGGA
GTAGATATTAAAACATACAGGGTTATATATGATGCTATAGAAGATATAAAATCAGCTATGCTAGGTATGTTAGAACCAGA
ATTTAAAGAAGTAGTTATAGGTACTGCAGAGGTTAGACAGGTTTATAAAATATCTAGTGTAGGAACTATAGCAGGAGCTT
ATATTCAAACAGGTAAATTAGCTAGAAACGCAGGAGCAAGAGTAATAAGAGATGGTATAGTAATATTTGAATCAGAACTT
GCATCTCTAAAGAGATTTAAAGATGATGCTAAAGAAGTTGCTCAAGGATATGAATGTGGACTTAGCATAGAAAAGTTTAA
TGACATAAAAGAAGGCGACATAATTGAATGCTTTATCATGGAAGAGATTAAAAAGAAAACACTATAA

Upstream 100 bases:

>100_bases
ACTTTATAAAAATATTAGCTGTAAAGGATGAAGGTATGGCTAATAAGTTATTAAAATTATGGAATGATAGCCAAGAGCAT
TAGTTTCGGGGGTGAATAAT

Downstream 100 bases:

>100_bases
AGTTCTCTTCTAAAAAAAGGAGTGGTTTTCATGGCAAAATATAGAGCAGGAAGAATAAATGAAGAAGTAAAAAAAGAAGT
GAGTAATATTATTCATAATG

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MAKIRVYELAKELNISSKELITLLEEEFSVEVKNHMSAIEDEDANLIKELLSGKEKSEKTKEEDDEIETTAKNPIKESMN
NKKSNKRDDKNEKVNTENAEDMGIITMTSDTITVKEISDKLEKSYAEVIKELMLMGVMASVNQEINFEMAEKLAAKFDME
ILKEDEDEKEDLEDILKDNEEEEYLQKRSPIITVMGHVDHGKTSLLDAIRKSKITSTEAGGITQHIGAYTVELNGEAITF
LDTPGHAAFTAMRARGAQVTDIVILVVAADDGIMPQTQEAISHCKAANVPLIVAINKIDRPGANIDKVKQELTEYGLVAE
DWGGDTICVPVSAHTKEGIDDLLEMILLSSEILELKANPNRKAKGTVVEAKLDKGRGPVATLLIQNGTLRVGDSIVVGST
YGRIRAMFNDKGRNIESAGPSTPVEILGLSEVPEAGDKFYQVKEEKTARGIADKRKEKIRDEYLQSTHKVSLEDLYNQIQ
EGTVKELGLIVKADVQGSVEALKQSLEKLSTEEVKVRVIHGGVGAINETDVTLATASNGIILGFNVRPDNNAIIASERDG
VDIKTYRVIYDAIEDIKSAMLGMLEPEFKEVVIGTAEVRQVYKISSVGTIAGAYIQTGKLARNAGARVIRDGIVIFESEL
ASLKRFKDDAKEVAQGYECGLSIEKFNDIKEGDIIECFIMEEIKKKTL

Sequences:

>Translated_688_residues
MAKIRVYELAKELNISSKELITLLEEEFSVEVKNHMSAIEDEDANLIKELLSGKEKSEKTKEEDDEIETTAKNPIKESMN
NKKSNKRDDKNEKVNTENAEDMGIITMTSDTITVKEISDKLEKSYAEVIKELMLMGVMASVNQEINFEMAEKLAAKFDME
ILKEDEDEKEDLEDILKDNEEEEYLQKRSPIITVMGHVDHGKTSLLDAIRKSKITSTEAGGITQHIGAYTVELNGEAITF
LDTPGHAAFTAMRARGAQVTDIVILVVAADDGIMPQTQEAISHCKAANVPLIVAINKIDRPGANIDKVKQELTEYGLVAE
DWGGDTICVPVSAHTKEGIDDLLEMILLSSEILELKANPNRKAKGTVVEAKLDKGRGPVATLLIQNGTLRVGDSIVVGST
YGRIRAMFNDKGRNIESAGPSTPVEILGLSEVPEAGDKFYQVKEEKTARGIADKRKEKIRDEYLQSTHKVSLEDLYNQIQ
EGTVKELGLIVKADVQGSVEALKQSLEKLSTEEVKVRVIHGGVGAINETDVTLATASNGIILGFNVRPDNNAIIASERDG
VDIKTYRVIYDAIEDIKSAMLGMLEPEFKEVVIGTAEVRQVYKISSVGTIAGAYIQTGKLARNAGARVIRDGIVIFESEL
ASLKRFKDDAKEVAQGYECGLSIEKFNDIKEGDIIECFIMEEIKKKTL
>Mature_687_residues
AKIRVYELAKELNISSKELITLLEEEFSVEVKNHMSAIEDEDANLIKELLSGKEKSEKTKEEDDEIETTAKNPIKESMNN
KKSNKRDDKNEKVNTENAEDMGIITMTSDTITVKEISDKLEKSYAEVIKELMLMGVMASVNQEINFEMAEKLAAKFDMEI
LKEDEDEKEDLEDILKDNEEEEYLQKRSPIITVMGHVDHGKTSLLDAIRKSKITSTEAGGITQHIGAYTVELNGEAITFL
DTPGHAAFTAMRARGAQVTDIVILVVAADDGIMPQTQEAISHCKAANVPLIVAINKIDRPGANIDKVKQELTEYGLVAED
WGGDTICVPVSAHTKEGIDDLLEMILLSSEILELKANPNRKAKGTVVEAKLDKGRGPVATLLIQNGTLRVGDSIVVGSTY
GRIRAMFNDKGRNIESAGPSTPVEILGLSEVPEAGDKFYQVKEEKTARGIADKRKEKIRDEYLQSTHKVSLEDLYNQIQE
GTVKELGLIVKADVQGSVEALKQSLEKLSTEEVKVRVIHGGVGAINETDVTLATASNGIILGFNVRPDNNAIIASERDGV
DIKTYRVIYDAIEDIKSAMLGMLEPEFKEVVIGTAEVRQVYKISSVGTIAGAYIQTGKLARNAGARVIRDGIVIFESELA
SLKRFKDDAKEVAQGYECGLSIEKFNDIKEGDIIECFIMEEIKKKTL

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=585, Percent_Identity=39.8290598290598, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI53729337, Length=585, Percent_Identity=39.8290598290598, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI84043963, Length=495, Percent_Identity=29.6969696969697, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI157426893, Length=228, Percent_Identity=25.8771929824561, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1789559, Length=576, Percent_Identity=53.125, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI2367247, Length=226, Percent_Identity=32.3008849557522, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788922, Length=235, Percent_Identity=29.7872340425532, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI48994988, Length=133, Percent_Identity=36.8421052631579, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1789737, Length=138, Percent_Identity=34.7826086956522, Blast_Score=63, Evalue=7e-11,
Organism=Escherichia coli, GI1790412, Length=138, Percent_Identity=34.7826086956522, Blast_Score=63, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=559, Percent_Identity=35.0626118067979, Blast_Score=315, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI212656558, Length=562, Percent_Identity=27.7580071174377, Blast_Score=147, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17557151, Length=268, Percent_Identity=26.4925373134328, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=241, Percent_Identity=31.1203319502075, Blast_Score=77, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324550, Length=543, Percent_Identity=38.8581952117864, Blast_Score=358, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6319282, Length=479, Percent_Identity=28.1837160751566, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324761, Length=242, Percent_Identity=27.6859504132231, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI28572034, Length=616, Percent_Identity=39.2857142857143, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24656849, Length=485, Percent_Identity=29.2783505154639, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI160714833, Length=112, Percent_Identity=56.25, Blast_Score=123, Evalue=5e-28,
Organism=Drosophila melanogaster, GI19922690, Length=227, Percent_Identity=30.8370044052863, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 75825; Mature: 75694

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIRVYELAKELNISSKELITLLEEEFSVEVKNHMSAIEDEDANLIKELLSGKEKSEKT
CCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHC
KEEDDEIETTAKNPIKESMNNKKSNKRDDKNEKVNTENAEDMGIITMTSDTITVKEISDK
CCCCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEHHHHHHH
LEKSYAEVIKELMLMGVMASVNQEINFEMAEKLAAKFDMEILKEDEDEKEDLEDILKDNE
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
EEEYLQKRSPIITVMGHVDHGKTSLLDAIRKSKITSTEAGGITQHIGAYTVELNGEAITF
HHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEEECCCEEEE
LDTPGHAAFTAMRARGAQVTDIVILVVAADDGIMPQTQEAISHCKAANVPLIVAINKIDR
EECCCCHHHHHHHHCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
PGANIDKVKQELTEYGLVAEDWGGDTICVPVSAHTKEGIDDLLEMILLSSEILELKANPN
CCCCHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEECCCCC
RKAKGTVVEAKLDKGRGPVATLLIQNGTLRVGDSIVVGSTYGRIRAMFNDKGRNIESAGP
CCCCCCEEEEEECCCCCCEEEEEEECCEEEECCEEEEECCHHHHEEEECCCCCCCCCCCC
STPVEILGLSEVPEAGDKFYQVKEEKTARGIADKRKEKIRDEYLQSTHKVSLEDLYNQIQ
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EGTVKELGLIVKADVQGSVEALKQSLEKLSTEEVKVRVIHGGVGAINETDVTLATASNGI
CCCHHHHCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEECCCCE
ILGFNVRPDNNAIIASERDGVDIKTYRVIYDAIEDIKSAMLGMLEPEFKEVVIGTAEVRQ
EEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECHHHHHH
VYKISSVGTIAGAYIQTGKLARNAGARVIRDGIVIFESELASLKRFKDDAKEVAQGYECG
HHHHHCCHHHHHHHHHCCHHHHHCCCHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
LSIEKFNDIKEGDIIECFIMEEIKKKTL
CCHHHHCCCCCCCEEEHHHHHHHHHHCC
>Mature Secondary Structure 
AKIRVYELAKELNISSKELITLLEEEFSVEVKNHMSAIEDEDANLIKELLSGKEKSEKT
CEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHC
KEEDDEIETTAKNPIKESMNNKKSNKRDDKNEKVNTENAEDMGIITMTSDTITVKEISDK
CCCCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEHHHHHHH
LEKSYAEVIKELMLMGVMASVNQEINFEMAEKLAAKFDMEILKEDEDEKEDLEDILKDNE
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
EEEYLQKRSPIITVMGHVDHGKTSLLDAIRKSKITSTEAGGITQHIGAYTVELNGEAITF
HHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEEECCCEEEE
LDTPGHAAFTAMRARGAQVTDIVILVVAADDGIMPQTQEAISHCKAANVPLIVAINKIDR
EECCCCHHHHHHHHCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
PGANIDKVKQELTEYGLVAEDWGGDTICVPVSAHTKEGIDDLLEMILLSSEILELKANPN
CCCCHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEECCCCC
RKAKGTVVEAKLDKGRGPVATLLIQNGTLRVGDSIVVGSTYGRIRAMFNDKGRNIESAGP
CCCCCCEEEEEECCCCCCEEEEEEECCEEEECCEEEEECCHHHHEEEECCCCCCCCCCCC
STPVEILGLSEVPEAGDKFYQVKEEKTARGIADKRKEKIRDEYLQSTHKVSLEDLYNQIQ
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EGTVKELGLIVKADVQGSVEALKQSLEKLSTEEVKVRVIHGGVGAINETDVTLATASNGI
CCCHHHHCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEECCCCE
ILGFNVRPDNNAIIASERDGVDIKTYRVIYDAIEDIKSAMLGMLEPEFKEVVIGTAEVRQ
EEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECHHHHHH
VYKISSVGTIAGAYIQTGKLARNAGARVIRDGIVIFESELASLKRFKDDAKEVAQGYECG
HHHHHCCHHHHHHHHHCCHHHHHCCCHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
LSIEKFNDIKEGDIIECFIMEEIKKKTL
CCHHHHCCCCCCCEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA