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The map label for this gene is rimO [H]

Identifier: 226949759

GI number: 226949759

Start: 2708913

End: 2710250

Strand: Reverse

Name: rimO [H]

Synonym: CLM_2699

Alternate gene names: 226949759

Gene position: 2710250-2708913 (Counterclockwise)

Preceding gene: 226949760

Following gene: 226949758

Centisome position: 65.22

GC content: 27.88

Gene sequence:

>1338_bases
GTGGAAAAAATTAAAGTAGCATTAGTTAGCTTAGGTTGTGATAAAAATAGAATTGATTCAGAACTTATGCTATACAAATT
AAATGAAGAAGCAGAACTAGTAAAAAATCCTAAAGAAGCACAGGTAATTATAGTAAATACCTGTGGATTTATTGAAACCG
CTAAGGAAGAATCTATAAATACCATTTTACAAATGGCTTCCTATAAAAAAACTCATAATTGCAAAGTATTAGTAGTTACA
GGATGTTTGACTCAAAGATATAAAGGGGAACTTAAAGAACTTATCCCAGAAATGGATATTATGTTAGGGGTAAATGATTA
TGATAAGTTACTTGAAAGTATAAAAGTCTTTTTGAAATCAGGAGAAAAAAGTTTTTATCATAAATATAGTGATACAAAAA
TAAATGAGGGAAATAGAATATTAACTACGCCAACATACACAGCCTATGTGAGAATAGCAGAAGGATGTAATAATTTTTGT
ACTTACTGTGCCATACCACGCATAAGAGGAAAGTATAGAAGTAGAAAAAAAGAAAATATATTAAAGGAAGTAGAAAATTT
AGCTAAACAAGGAGTTAAAGAAATAATATTGATAGCTCAAGATACAACCATGTATGGAATAGATATATATGGCAAAAAAG
TTTTACATGAGCTTCTAAGAGATATTTCTAAAGTTGAAGGTGTAAAGTGGATAAGATTATTATACTGTTACCCAGAGGAA
ATAACTAAGGAACTTATAGAAGAGATTAAAAATAATGATAAGGTATGTAAATATTTAGATTTGCCTATACAGCAGATAAG
TAATTCTGTTTTAAAAAGAATGGGAAGAAAAACTACAAAGGAAACTATCATAAATATAATAAAAAAATTAAGAAAAGAAA
TAGAGGGTATTACTTTAAGGACTTCATTAATAGTAGGATTTCCAGGTGAAACAGAAGGAGAGTTTAGTGAACTTAAAGAA
TTTGTTTCAGATGTAAAATTAGATAAATTAGGTGTATTTAAATATTCAAAGGAAGAAGGAACGTCAGCAGCTCTTATGAA
GGAACAAATAGATGAAGAAATAAAAGAAAAAAGAGAAGAAGAAATAATGATACTTCAGCAAAGTATATCTAAAGACATAA
ATAAAGAGAAAATAGGTAAAATATATGAAGTCATAGTAGAAGGTACCAAAGAAGATATGTATTATGGGAGAAATTATGAG
ATGTCACCGGAGATAGATGGAGAAATTTACTTTGAAAAGGATGAAAACGTAAAAATTGGTGATATAATAAAGGTGAAGGT
AACTCATAGTTTAGAATATGATTTAATAGGAGTTGTTTATAATGAACTTAGCAAATAA

Upstream 100 bases:

>100_bases
ATGTAAATACTTTTAAAATTCAAGTTACTTAATACTTGTAAAATTTCTTAAGTAGATTTAAAATGTTTCTTGTGACATAA
AAAATGTAGGAGGGGAACAG

Downstream 100 bases:

>100_bases
ACTAACTATATTAAGAATTTTTTTAGTTCCAATATTTCTTATATTTATATCAGTAAAAAACATACCCTATGGTACAGTAA
TAGCTACTGCAGTTTTCGTA

Product: RNA modification enzyme, MiaB family

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKNPKEAQVIIVNTCGFIETAKEESINTILQMASYKKTHNCKVLVVT
GCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKSGEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFC
TYCAIPRIRGKYRSRKKENILKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLRTSLIVGFPGETEGEFSELKE
FVSDVKLDKLGVFKYSKEEGTSAALMKEQIDEEIKEKREEEIMILQQSISKDINKEKIGKIYEVIVEGTKEDMYYGRNYE
MSPEIDGEIYFEKDENVKIGDIIKVKVTHSLEYDLIGVVYNELSK

Sequences:

>Translated_445_residues
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKNPKEAQVIIVNTCGFIETAKEESINTILQMASYKKTHNCKVLVVT
GCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKSGEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFC
TYCAIPRIRGKYRSRKKENILKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLRTSLIVGFPGETEGEFSELKE
FVSDVKLDKLGVFKYSKEEGTSAALMKEQIDEEIKEKREEEIMILQQSISKDINKEKIGKIYEVIVEGTKEDMYYGRNYE
MSPEIDGEIYFEKDENVKIGDIIKVKVTHSLEYDLIGVVYNELSK
>Mature_445_residues
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKNPKEAQVIIVNTCGFIETAKEESINTILQMASYKKTHNCKVLVVT
GCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKSGEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFC
TYCAIPRIRGKYRSRKKENILKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLRTSLIVGFPGETEGEFSELKE
FVSDVKLDKLGVFKYSKEEGTSAALMKEQIDEEIKEKREEEIMILQQSISKDINKEKIGKIYEVIVEGTKEDMYYGRNYE
MSPEIDGEIYFEKDENVKIGDIIKVKVTHSLEYDLIGVVYNELSK

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=488, Percent_Identity=28.2786885245902, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=28.4823284823285, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI93277076, Length=392, Percent_Identity=27.2959183673469, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1787057, Length=444, Percent_Identity=38.0630630630631, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1786882, Length=425, Percent_Identity=30.3529411764706, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17553146, Length=473, Percent_Identity=24.5243128964059, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71996771, Length=371, Percent_Identity=28.0323450134771, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356207, Length=470, Percent_Identity=26.8085106382979, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=25.9911894273128, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 51361; Mature: 51361

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKNPKEAQVIIVNTCGFIETAKEESIN
CCCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
TILQMASYKKTHNCKVLVVTGCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKS
HHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHC
GEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFCTYCAIPRIRGKYRSRKKENI
CCHHHHHCCCCCEECCCCEEEECCCCEEEEEEHHCCCHHHHHHCCHHHHHHHHHHHHHHH
LKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
HHHHHHHHHHCHHHEEEEEECCEEEEEEHHHHHHHHHHHHHHHHHCCHHEEHHEEECCHH
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLR
HHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEE
TSLIVGFPGETEGEFSELKEFVSDVKLDKLGVFKYSKEEGTSAALMKEQIDEEIKEKREE
EEEEEECCCCCCCCHHHHHHHHHHCCCCHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
EIMILQQSISKDINKEKIGKIYEVIVEGTKEDMYYGRNYEMSPEIDGEIYFEKDENVKIG
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHEECCCCCCCCCCCCEEEEECCCCEEEC
DIIKVKVTHSLEYDLIGVVYNELSK
CEEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKNPKEAQVIIVNTCGFIETAKEESIN
CCCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
TILQMASYKKTHNCKVLVVTGCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKS
HHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHC
GEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFCTYCAIPRIRGKYRSRKKENI
CCHHHHHCCCCCEECCCCEEEECCCCEEEEEEHHCCCHHHHHHCCHHHHHHHHHHHHHHH
LKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
HHHHHHHHHHCHHHEEEEEECCEEEEEEHHHHHHHHHHHHHHHHHCCHHEEHHEEECCHH
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLR
HHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEE
TSLIVGFPGETEGEFSELKEFVSDVKLDKLGVFKYSKEEGTSAALMKEQIDEEIKEKREE
EEEEEECCCCCCCCHHHHHHHHHHCCCCHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
EIMILQQSISKDINKEKIGKIYEVIVEGTKEDMYYGRNYEMSPEIDGEIYFEKDENVKIG
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHEECCCCCCCCCCCCEEEEECCCCEEEC
DIIKVKVTHSLEYDLIGVVYNELSK
CEEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA