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The map label for this gene is cca [H]

Identifier: 226949676

GI number: 226949676

Start: 2644605

End: 2645957

Strand: Reverse

Name: cca [H]

Synonym: CLM_2616

Alternate gene names: 226949676

Gene position: 2645957-2644605 (Counterclockwise)

Preceding gene: 226949677

Following gene: 226949675

Centisome position: 63.68

GC content: 25.35

Gene sequence:

>1353_bases
ATGCTAAATAAAAATATAAAAATTCAAATACCTAAAGGGGTAGAGTATATTATAAATATTTTACAAGAAAATGGTTATGA
AGCATGTATAGTAGGTGGTGCAGTTAGAGATAGCTTACTTGAAAGAAAAGTTAATGATTGGGATATAACTACTAGTGCCA
ATCCACAAGAAGTAGTGAACATATTTGAAAATTTAGGATATAAAATTATACCTACAGGATTGAAACATGGAACGGTAACA
ATATTAATTAATTCTATAGGTTATGAAGTAACTACATTTAGAATTGACGGAGAATATGAAGATAACAGACGTCCTAAAGA
AGTTAAGTTTACATCAAATTTAAAAGAAGATTTAAAGCGTAGAGATTTAACTATTAATGCAATGGCTTACAATGACAAAA
TAGGATTGGTAGATTATTTTCATGGATTGGAAGATTTAAATAATAAAATAATTAGATGCGTAGGAAATTCAAAAGATAGG
TTTAATGAAGATTCACTAAGAATGTTAAGATGTATAAGATTTGCTTCACAATTAAATTTTCATATGGAAGAATCTACAAA
ATTTAACATAAGAGAATTAAGTAAAAATATAGCTAATGTATCTATGGAAAGAACCAGAGACGAATTATGCAAAATATTAG
TAAGCAGCCAACCGGTTTATGGCATAAGAAATATAGTTGAATTAAATTTAATGGATTATATTATTCCTGAACTAAAAGCT
TGTGTAGGCTTTGAGCAACATAATATACATCATGATAAAGATATCTATGGTCATATATTATCTGTAGTAGAAAATGTACC
AAATAAATTAGAATTAAGATTAGCAGCATTATTACATGATATAGGAAAACCTAGGTGTTTTAGTATAGGAGACAATGGAC
AAGGACATTTTTATGGTCATCAAAAGATAAGTGCGGATATGACTAAAGATATATTAAAAAGATTAAAATTTGATAATAAA
ACAATATATAAGGTAGATAAATTAGTTTATAATCATATGACTAGATACAATAAATTAAGAACTCCTAACATAAAAAAGTT
TATTAATAAAGTAGGAATTGATAATTTAGATGATTTATTTGAGCTACAAGTAGCTGATATAAAAGGAAGTGCTAAAGAAT
ATCATAGTTTCGATAATGTATTAAATCTAAAAATAAAGTGTGAAAAAATACTTAGTGAAAAACAACCTTTAACAATTAAG
GATTTAGATATTAATGGATATGACTTAATGAAGTTAGGAATAAACCAAGGAAGAGAAATAGGTATTATGTTAAATAAATT
ATTGGATATAATTTTAGAAAATCCTAATTTAAATAATAAAGAAGACTTAATTAAAATAGTTGAAAGTATCTAA

Upstream 100 bases:

>100_bases
GATTTTAAATATGCGTCTGATAATACAGATTTACCCAACATGCCTAATTATAAAAAAGTAGAAGAGCTAGTTATAGAAAT
TAATAAAGGTGTGATAAATA

Downstream 100 bases:

>100_bases
AAAAGATATTTTATTTAAATATTCTAATATCGTAATATGAAATAAAGGCGGTGCGACAAGAAGTCGCTAAATGGTAGTGT
TTCGGAAGAAACTGCGTTAG

Product: polyA polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLNKNIKIQIPKGVEYIINILQENGYEACIVGGAVRDSLLERKVNDWDITTSANPQEVVNIFENLGYKIIPTGLKHGTVT
ILINSIGYEVTTFRIDGEYEDNRRPKEVKFTSNLKEDLKRRDLTINAMAYNDKIGLVDYFHGLEDLNNKIIRCVGNSKDR
FNEDSLRMLRCIRFASQLNFHMEESTKFNIRELSKNIANVSMERTRDELCKILVSSQPVYGIRNIVELNLMDYIIPELKA
CVGFEQHNIHHDKDIYGHILSVVENVPNKLELRLAALLHDIGKPRCFSIGDNGQGHFYGHQKISADMTKDILKRLKFDNK
TIYKVDKLVYNHMTRYNKLRTPNIKKFINKVGIDNLDDLFELQVADIKGSAKEYHSFDNVLNLKIKCEKILSEKQPLTIK
DLDINGYDLMKLGINQGREIGIMLNKLLDIILENPNLNNKEDLIKIVESI

Sequences:

>Translated_450_residues
MLNKNIKIQIPKGVEYIINILQENGYEACIVGGAVRDSLLERKVNDWDITTSANPQEVVNIFENLGYKIIPTGLKHGTVT
ILINSIGYEVTTFRIDGEYEDNRRPKEVKFTSNLKEDLKRRDLTINAMAYNDKIGLVDYFHGLEDLNNKIIRCVGNSKDR
FNEDSLRMLRCIRFASQLNFHMEESTKFNIRELSKNIANVSMERTRDELCKILVSSQPVYGIRNIVELNLMDYIIPELKA
CVGFEQHNIHHDKDIYGHILSVVENVPNKLELRLAALLHDIGKPRCFSIGDNGQGHFYGHQKISADMTKDILKRLKFDNK
TIYKVDKLVYNHMTRYNKLRTPNIKKFINKVGIDNLDDLFELQVADIKGSAKEYHSFDNVLNLKIKCEKILSEKQPLTIK
DLDINGYDLMKLGINQGREIGIMLNKLLDIILENPNLNNKEDLIKIVESI
>Mature_450_residues
MLNKNIKIQIPKGVEYIINILQENGYEACIVGGAVRDSLLERKVNDWDITTSANPQEVVNIFENLGYKIIPTGLKHGTVT
ILINSIGYEVTTFRIDGEYEDNRRPKEVKFTSNLKEDLKRRDLTINAMAYNDKIGLVDYFHGLEDLNNKIIRCVGNSKDR
FNEDSLRMLRCIRFASQLNFHMEESTKFNIRELSKNIANVSMERTRDELCKILVSSQPVYGIRNIVELNLMDYIIPELKA
CVGFEQHNIHHDKDIYGHILSVVENVPNKLELRLAALLHDIGKPRCFSIGDNGQGHFYGHQKISADMTKDILKRLKFDNK
TIYKVDKLVYNHMTRYNKLRTPNIKKFINKVGIDNLDDLFELQVADIKGSAKEYHSFDNVLNLKIKCEKILSEKQPLTIK
DLDINGYDLMKLGINQGREIGIMLNKLLDIILENPNLNNKEDLIKIVESI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=242, Percent_Identity=37.603305785124, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI87081691, Length=231, Percent_Identity=30.7359307359307, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789436, Length=315, Percent_Identity=27.9365079365079, Blast_Score=101, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71995920, Length=213, Percent_Identity=33.8028169014084, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321016, Length=298, Percent_Identity=29.1946308724832, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281360112, Length=286, Percent_Identity=33.9160839160839, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI21357337, Length=286, Percent_Identity=33.9160839160839, Blast_Score=125, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 51839; Mature: 51839

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKNIKIQIPKGVEYIINILQENGYEACIVGGAVRDSLLERKVNDWDITTSANPQEVVN
CCCCCEEEECCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCEECCCCCHHHHHH
IFENLGYKIIPTGLKHGTVTILINSIGYEVTTFRIDGEYEDNRRPKEVKFTSNLKEDLKR
HHHHCCCEEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHH
RDLTINAMAYNDKIGLVDYFHGLEDLNNKIIRCVGNSKDRFNEDSLRMLRCIRFASQLNF
CCCEEEEEEECCCCCHHHHHHHHHHHCHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
HMEESTKFNIRELSKNIANVSMERTRDELCKILVSSQPVYGIRNIVELNLMDYIIPELKA
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
CVGFEQHNIHHDKDIYGHILSVVENVPNKLELRLAALLHDIGKPRCFSIGDNGQGHFYGH
HHCCHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEC
QKISADMTKDILKRLKFDNKTIYKVDKLVYNHMTRYNKLRTPNIKKFINKVGIDNLDDLF
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHH
ELQVADIKGSAKEYHSFDNVLNLKIKCEKILSEKQPLTIKDLDINGYDLMKLGINQGREI
HEEEECCCCCHHHHHHHHHHEEEEEEEHHHHCCCCCCEEEEECCCCEEEEEECCCCCCCH
GIMLNKLLDIILENPNLNNKEDLIKIVESI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
>Mature Secondary Structure
MLNKNIKIQIPKGVEYIINILQENGYEACIVGGAVRDSLLERKVNDWDITTSANPQEVVN
CCCCCEEEECCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCEECCCCCHHHHHH
IFENLGYKIIPTGLKHGTVTILINSIGYEVTTFRIDGEYEDNRRPKEVKFTSNLKEDLKR
HHHHCCCEEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHH
RDLTINAMAYNDKIGLVDYFHGLEDLNNKIIRCVGNSKDRFNEDSLRMLRCIRFASQLNF
CCCEEEEEEECCCCCHHHHHHHHHHHCHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
HMEESTKFNIRELSKNIANVSMERTRDELCKILVSSQPVYGIRNIVELNLMDYIIPELKA
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
CVGFEQHNIHHDKDIYGHILSVVENVPNKLELRLAALLHDIGKPRCFSIGDNGQGHFYGH
HHCCHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEC
QKISADMTKDILKRLKFDNKTIYKVDKLVYNHMTRYNKLRTPNIKKFINKVGIDNLDDLF
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHH
ELQVADIKGSAKEYHSFDNVLNLKIKCEKILSEKQPLTIKDLDINGYDLMKLGINQGREI
HEEEECCCCCHHHHHHHHHHEEEEEEEHHHHCCCCCCEEEEECCCCEEEEEECCCCCCCH
GIMLNKLLDIILENPNLNNKEDLIKIVESI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA