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The map label for this gene is def [H]

Identifier: 226949660

GI number: 226949660

Start: 2629717

End: 2630169

Strand: Reverse

Name: def [H]

Synonym: CLM_2597

Alternate gene names: 226949660

Gene position: 2630169-2629717 (Counterclockwise)

Preceding gene: 226949661

Following gene: 226949659

Centisome position: 63.3

GC content: 30.02

Gene sequence:

>453_bases
ATGGCATTAAGACAAATTAGATTGTTTGATGATGAAATATTAAGAAAAGAGAGTAAGGTAGTTGAGATTGTAGACGATAA
AATAAGACAAATATTAAATGATATGACAGATACTATGTATAATACAGAAAATGGAGGAGGATTAGCAGCCCCACAAGTAG
GTATATTGAAGCGATTAGTTGTAATAGATATGGGACAAGGACTTATAAAATTAGTGAATCCAAAGATAATTAAACAAGAA
GGAACTCAAGAAGTTATTGAAGGGTGTCTGAGTATTCCTAATAAATTTGGAAAGTTAATAAGACCTGCAAAAGTAACAGT
ACAAGCATTAAATGAAAATGGAGAGGAAATTATATTAACAGGTACAGGGGATTTAGCAAAATGTTTTTGCCATGAAATAG
ACCATTTAGAAGGTATTCTTTTTACTGATTTAGTAACTGAATACATAATCTAA

Upstream 100 bases:

>100_bases
TGATATTGCAAAAGTATTCCATGACACTATAGAAGAAGTTTTCCTTTTTGAAGAAAGTGAAAGAAAATCAAGAGCAGAGA
AAAGTAGAGGTGTAGTTTAA

Downstream 100 bases:

>100_bases
TATCATTTATATTATAAATTTATTTGATTGCAAAAACTTGCGCTAGGAAAAGAAATCTCTTTTCTTATTAGTATATTAAA
AAAATAAATAATTTTAACAA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MALRQIRLFDDEILRKESKVVEIVDDKIRQILNDMTDTMYNTENGGGLAAPQVGILKRLVVIDMGQGLIKLVNPKIIKQE
GTQEVIEGCLSIPNKFGKLIRPAKVTVQALNENGEEIILTGTGDLAKCFCHEIDHLEGILFTDLVTEYII

Sequences:

>Translated_150_residues
MALRQIRLFDDEILRKESKVVEIVDDKIRQILNDMTDTMYNTENGGGLAAPQVGILKRLVVIDMGQGLIKLVNPKIIKQE
GTQEVIEGCLSIPNKFGKLIRPAKVTVQALNENGEEIILTGTGDLAKCFCHEIDHLEGILFTDLVTEYII
>Mature_149_residues
ALRQIRLFDDEILRKESKVVEIVDDKIRQILNDMTDTMYNTENGGGLAAPQVGILKRLVVIDMGQGLIKLVNPKIIKQEG
TQEVIEGCLSIPNKFGKLIRPAKVTVQALNENGEEIILTGTGDLAKCFCHEIDHLEGILFTDLVTEYII

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=41.6107382550336, Blast_Score=107, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 16719; Mature: 16588

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRQIRLFDDEILRKESKVVEIVDDKIRQILNDMTDTMYNTENGGGLAAPQVGILKRLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
VIDMGQGLIKLVNPKIIKQEGTQEVIEGCLSIPNKFGKLIRPAKVTVQALNENGEEIILT
HHHCCCHHHHHHCCHHHHCCCHHHHHHHHHHCHHHHHHHHCCHHHHHHHHCCCCCEEEEE
GTGDLAKCFCHEIDHLEGILFTDLVTEYII
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALRQIRLFDDEILRKESKVVEIVDDKIRQILNDMTDTMYNTENGGGLAAPQVGILKRLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
VIDMGQGLIKLVNPKIIKQEGTQEVIEGCLSIPNKFGKLIRPAKVTVQALNENGEEIILT
HHHCCCHHHHHHCCHHHHCCCHHHHHHHHHHCHHHHHHHHCCHHHHHHHHCCCCCEEEEE
GTGDLAKCFCHEIDHLEGILFTDLVTEYII
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]