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The map label for this gene is ygaF [C]

Identifier: 226949516

GI number: 226949516

Start: 2532418

End: 2533866

Strand: Reverse

Name: ygaF [C]

Synonym: CLM_2451

Alternate gene names: 226949516

Gene position: 2533866-2532418 (Counterclockwise)

Preceding gene: 226949518

Following gene: 226949515

Centisome position: 60.98

GC content: 31.75

Gene sequence:

>1449_bases
ATGTATGATGTTAGTATTATAGGTGCTGGAGTTGTTGGATCATCCATTGCTAGAGAGTTATCAAAGTATAATTTAAAAGT
ATGTCTTATAGAAAAAGAGGAGGATGTTGGAACAGGAGCTTCCAAAGCAAATAGTGGTATTGTTCATGGTGGATATGTAG
CTAAATATGGAACTCTAAAGGGAGAATTATGTATAAGAGGAAACAGTATGTACAATAAGCTGGAAAAAGAGCTTAACTTT
GGATATAGAAATCCAGGTGCATTAGTAATAGGCTTTGATGAAGATGATGAAAATAGAATAAAAAAATTATATGAAAATGG
TATCAAAGTAGGATGCAATGATTTGGAGATAATATATGGGGATAAAATAAAAGAATTGGAGCCTCATATAAATAAAGATG
TTAGAGTTGCATTATATGCTAAAAGTGTTGGTGTAGCTTCTCCTTATGAAATGACTATAGCCCTTGCCGAAAATGCAATA
GAAAATAGAGTAGATTTAAAATTAGAAACAAAAGTATTAGCTATAGATAAAGAACATGAAGCTTTTATAATAAATACTAA
TAAAGGAGAAATAAAAAGTAAGTATATTGTAAATGCTGCTGGATTATATAGCGATAAGATAGCCAATATGCTAGAGATGG
ATGATTTTAAAATATTGCCAAGAAGAGGGCAGTATGTACTTTCTACCAAAGATCAAGGTTATCTTGTAAATAAGGTTATA
TTCCAAGTGCCAACAGAAAAAGGAAAAGGAATATTAGTTACTACTACTTATCATGGAAACTTTATGATAGGTCCTGATGC
ACAGGAGGTAGTAGACAAAGAAGATATAGGAACAGATATAGAAAGTATAGAATATATAATAAAAACAGCTAGAAAATCTA
TACCAGATTTTGACGTAAGAAAATCTTTAACTACCTTTGCAGGAATAAGAGCAATTAGTAGTACAGGAGATTTTGTAATA
GGAGAAACTAAAGTTAAAGGCTTTATAAATGCAGCAGGTATAGATTCACCAGGTCTTACATCTTCACCAGCTATAGCAGA
AAAAATAGTAGGAATTCTTAAGGAAGCTGGATTGGAATTAATAAAAAATGAAAAATTCAATCCCTACAGAAAGCCCATAA
TTATTAAAAAAGATAAATCCTTTGATGGAAAGATAGATGATGAAGATCCAACTAAAAACATAATATGTAGGTGTGAAAAG
GTAACGGAATCAGAAATAATAGATGCAATGAAAAGAGGAATTCCTGTAAAATCTACAGATGCAATAAAAAGACGTACAAG
GGCAGGTATGGGGTTTTGCCAAGGTAATTTCTGTAGACCAAGAGTAAAAGCTATAATAGCAAGAGAAATGGGTATACCTG
CAGAACAAGTTACTGTAAGAGGCAAAGAGGGAGGAGAGCCTCCTAAAAGAGTAAATATTAATGTAATAAGAAAAATTCAA
GGTGTTTGA

Upstream 100 bases:

>100_bases
TTATTTTAGGATGTAGAATAGTATTAAGAAAATAATAGAAAATATTAAAAGATATATATTATTAATAACCTTTAAATAAT
AAGAATAAGAGGGTGAGACT

Downstream 100 bases:

>100_bases
TTTCTTTTAAATATTAATAAAATATAAGGAAATGCCTAATATGTAAATAAGGGCTGCTAATAAAAAAGTAGCTTTTATTT
ACATTCTAGGTATAACTTTA

Product: FAD/[2Fe-2S]-binding family oxidoreductase

Products: glycerone phosphate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MYDVSIIGAGVVGSSIARELSKYNLKVCLIEKEEDVGTGASKANSGIVHGGYVAKYGTLKGELCIRGNSMYNKLEKELNF
GYRNPGALVIGFDEDDENRIKKLYENGIKVGCNDLEIIYGDKIKELEPHINKDVRVALYAKSVGVASPYEMTIALAENAI
ENRVDLKLETKVLAIDKEHEAFIINTNKGEIKSKYIVNAAGLYSDKIANMLEMDDFKILPRRGQYVLSTKDQGYLVNKVI
FQVPTEKGKGILVTTTYHGNFMIGPDAQEVVDKEDIGTDIESIEYIIKTARKSIPDFDVRKSLTTFAGIRAISSTGDFVI
GETKVKGFINAAGIDSPGLTSSPAIAEKIVGILKEAGLELIKNEKFNPYRKPIIIKKDKSFDGKIDDEDPTKNIICRCEK
VTESEIIDAMKRGIPVKSTDAIKRRTRAGMGFCQGNFCRPRVKAIIAREMGIPAEQVTVRGKEGGEPPKRVNINVIRKIQ
GV

Sequences:

>Translated_482_residues
MYDVSIIGAGVVGSSIARELSKYNLKVCLIEKEEDVGTGASKANSGIVHGGYVAKYGTLKGELCIRGNSMYNKLEKELNF
GYRNPGALVIGFDEDDENRIKKLYENGIKVGCNDLEIIYGDKIKELEPHINKDVRVALYAKSVGVASPYEMTIALAENAI
ENRVDLKLETKVLAIDKEHEAFIINTNKGEIKSKYIVNAAGLYSDKIANMLEMDDFKILPRRGQYVLSTKDQGYLVNKVI
FQVPTEKGKGILVTTTYHGNFMIGPDAQEVVDKEDIGTDIESIEYIIKTARKSIPDFDVRKSLTTFAGIRAISSTGDFVI
GETKVKGFINAAGIDSPGLTSSPAIAEKIVGILKEAGLELIKNEKFNPYRKPIIIKKDKSFDGKIDDEDPTKNIICRCEK
VTESEIIDAMKRGIPVKSTDAIKRRTRAGMGFCQGNFCRPRVKAIIAREMGIPAEQVTVRGKEGGEPPKRVNINVIRKIQ
GV
>Mature_482_residues
MYDVSIIGAGVVGSSIARELSKYNLKVCLIEKEEDVGTGASKANSGIVHGGYVAKYGTLKGELCIRGNSMYNKLEKELNF
GYRNPGALVIGFDEDDENRIKKLYENGIKVGCNDLEIIYGDKIKELEPHINKDVRVALYAKSVGVASPYEMTIALAENAI
ENRVDLKLETKVLAIDKEHEAFIINTNKGEIKSKYIVNAAGLYSDKIANMLEMDDFKILPRRGQYVLSTKDQGYLVNKVI
FQVPTEKGKGILVTTTYHGNFMIGPDAQEVVDKEDIGTDIESIEYIIKTARKSIPDFDVRKSLTTFAGIRAISSTGDFVI
GETKVKGFINAAGIDSPGLTSSPAIAEKIVGILKEAGLELIKNEKFNPYRKPIIIKKDKSFDGKIDDEDPTKNIICRCEK
VTESEIIDAMKRGIPVKSTDAIKRRTRAGMGFCQGNFCRPRVKAIIAREMGIPAEQVTVRGKEGGEPPKRVNINVIRKIQ
GV

Specific function: Unknown

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI13376331, Length=278, Percent_Identity=26.978417266187, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI24797151, Length=237, Percent_Identity=28.2700421940928, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI194306651, Length=360, Percent_Identity=21.9444444444444, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI87082148, Length=257, Percent_Identity=24.5136186770428, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788574, Length=482, Percent_Identity=21.7842323651452, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17565764, Length=402, Percent_Identity=28.6069651741293, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24585081, Length=407, Percent_Identity=28.009828009828, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.1.99.5

Molecular weight: Translated: 53138; Mature: 53138

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDVSIIGAGVVGSSIARELSKYNLKVCLIEKEEDVGTGASKANSGIVHGGYVAKYGTLK
CEEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEECCEEEEECCCC
GELCIRGNSMYNKLEKELNFGYRNPGALVIGFDEDDENRIKKLYENGIKVGCNDLEIIYG
CEEEEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEECCCCEEEEEC
DKIKELEPHINKDVRVALYAKSVGVASPYEMTIALAENAIENRVDLKLETKVLAIDKEHE
CCHHHCCCCCCCCEEEEEEEECCCCCCCCEEEEEEHHHHHCCCCCEEEEEEEEEEECCCC
AFIINTNKGEIKSKYIVNAAGLYSDKIANMLEMDDFKILPRRGQYVLSTKDQGYLVNKVI
EEEEECCCCCCCCEEEEEECCHHHHHHHHHHHCCCEEECCCCCCEEEECCCCCEEEEEEE
FQVPTEKGKGILVTTTYHGNFMIGPDAQEVVDKEDIGTDIESIEYIIKTARKSIPDFDVR
EEECCCCCCEEEEEEEECCCEEECCCHHHHHCHHHCCCCHHHHHHHHHHHHHCCCCHHHH
KSLTTFAGIRAISSTGDFVIGETKVKGFINAAGIDSPGLTSSPAIAEKIVGILKEAGLEL
HHHHHHHHHHHCCCCCCEEEECHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHH
IKNEKFNPYRKPIIIKKDKSFDGKIDDEDPTKNIICRCEKVTESEIIDAMKRGIPVKSTD
HHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHEEEHHCCHHHHHHHHHCCCCCCCHH
AIKRRTRAGMGFCQGNFCRPRVKAIIAREMGIPAEQVTVRGKEGGEPPKRVNINVIRKIQ
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHEEEECCCCCCCCCEECCEEEEEEC
GV
CC
>Mature Secondary Structure
MYDVSIIGAGVVGSSIARELSKYNLKVCLIEKEEDVGTGASKANSGIVHGGYVAKYGTLK
CEEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEECCEEEEECCCC
GELCIRGNSMYNKLEKELNFGYRNPGALVIGFDEDDENRIKKLYENGIKVGCNDLEIIYG
CEEEEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEECCCCEEEEEC
DKIKELEPHINKDVRVALYAKSVGVASPYEMTIALAENAIENRVDLKLETKVLAIDKEHE
CCHHHCCCCCCCCEEEEEEEECCCCCCCCEEEEEEHHHHHCCCCCEEEEEEEEEEECCCC
AFIINTNKGEIKSKYIVNAAGLYSDKIANMLEMDDFKILPRRGQYVLSTKDQGYLVNKVI
EEEEECCCCCCCCEEEEEECCHHHHHHHHHHHCCCEEECCCCCCEEEECCCCCEEEEEEE
FQVPTEKGKGILVTTTYHGNFMIGPDAQEVVDKEDIGTDIESIEYIIKTARKSIPDFDVR
EEECCCCCCEEEEEEEECCCEEECCCHHHHHCHHHCCCCHHHHHHHHHHHHHCCCCHHHH
KSLTTFAGIRAISSTGDFVIGETKVKGFINAAGIDSPGLTSSPAIAEKIVGILKEAGLEL
HHHHHHHHHHHCCCCCCEEEECHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHH
IKNEKFNPYRKPIIIKKDKSFDGKIDDEDPTKNIICRCEKVTESEIIDAMKRGIPVKSTD
HHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHEEEHHCCHHHHHHHHHCCCCCCCHH
AIKRRTRAGMGFCQGNFCRPRVKAIIAREMGIPAEQVTVRGKEGGEPPKRVNINVIRKIQ
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHEEEECCCCCCCCCEECCEEEEEEC
GV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sn-glycerol 3-phosphate; acceptor

Specific reaction: sn-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]