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The map label for this gene is vanSB [H]

Identifier: 226949266

GI number: 226949266

Start: 2246772

End: 2248211

Strand: Reverse

Name: vanSB [H]

Synonym: CLM_2185

Alternate gene names: 226949266

Gene position: 2248211-2246772 (Counterclockwise)

Preceding gene: 226949267

Following gene: 226949262

Centisome position: 54.1

GC content: 25.83

Gene sequence:

>1440_bases
TTGAGAAATAAATTAAAAAAAAGTATAGTATATAAGCTATTTTTAGTAACTACATTATTATTAGTAATAAGTTCTATATG
CACTTACTCTATAATTTATATGCTGCTACCTAATTATTATCATAAATATAAAAAAATGACTATAGAAAATCAATTAGATA
GTATAGTAGATAATGTACCTAAATTAGATATAAATAATATTGAAGAATATTTGGGGCAAATTTCATTTAATAATAATGCC
AATATAGTAGTAACAGACGAATATGGAAACGTTATTTATTTTACTAATGTTCTTCAAAAAGGTCTTGTAACTATTCCTAA
AAAAGAAGATTTATCTCATTTTAAAAATATAGAAAAAACTAATACTAGATCAGTGTATACAAGTTATAGAAAAATAAAAT
TCTATAATAGTGAAGAAAAGTATAATTTATATTTAACTGCTCCGCTTCAGCCTGTGTCAGAGGCATCTAAAGTTTTATTT
TTACTTATACCTTACATAGGATTAGTTGTAATTTTAATTTCTGTAATAGGGGGATTAATTTATTCTAAAGTTATATCTAA
GCCGTTAATAAGCATGAATAAAGTAGCTAAAGAAATGGCTAAATTAGATTTTACTAAGAAGTGTACCGTAAAAGGGGAGG
ATGAAATAGGAGAACTTTCTCAAAGTTTAAATGATCTGTCCAATAATCTAAGGATCTCTATGGAGGAACTCCAAAGAGCT
AATGAACAATTATTAGATGATATTGCTAAAGAGAGGGAAATAGAGAAAAAAAGAAGAGAATTTATAGCCACCATATCTCA
TGAATTAAAAACCCCTATAACTATTTTAAAGGGGCAAATAGAAGGAATGCTATCTAATATAGGTATTTATAAAGACAGGG
ATAAATATTTAAAGAGAAACCTTGAAGTTTTAAATGATATGGAATATATGGTTAAGGAAACTTTAGAAATATCAAAATTA
GAGTCGCAGGGCTTTAAACCTCGAAAAGAGCAGATTTCCTTAAGTAAAATAGTTGAAGAATGTATATATAATATCTCCTT
TATAGCTAAAAGAAAGAATATATTCATAGATAAAAATATAAACGAAGATTTATTTGTTCGCGGAGATAGTAAATTATTAA
AGAAAGTAGTGAACAATATAATAACTAATGCTATTAATCATTCTCCAGAAAGTGAAAAAGTTTATGCAAATCTTCATGAA
GAAAAAGATGAAATTGTATTAAAGGTGGAAAATACAGGAATCTATATAGAAGAAAATGAACTTAAAGAAATATTTAAACC
TTTTTATAGAATTGAAAAATCTCGTAATAGGAAAAGTGGAGGTAGTGGACTTGGACTTTATATAGTAAAAATGATTTTAG
ATGCACACAATGGAAAATATTCTATTTCAAACAATGAAAAAGGAGTGGAATTTAAGCTTTGCCTAAAAAAGTATAGTTAA

Upstream 100 bases:

>100_bases
TATTTTGGAGATACCCGTGTTATAGATACTCATATCAAAAATTTAAGACAAAAACTAAACATAGATTATATAAAGACCAT
AAGGGGAGTGGGGTATAAAA

Downstream 100 bases:

>100_bases
AAGAACTAATGATTATTAAGTATAAAAGTTCAATAATTTCGAAGTAATTAAAAAGGTTTATAGATATAGCAAAGACTTAT
TTATAGTAACTCAAAATTTA

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin B-type resistance protein vanSB; Vancomycin histidine protein kinase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MRNKLKKSIVYKLFLVTTLLLVISSICTYSIIYMLLPNYYHKYKKMTIENQLDSIVDNVPKLDINNIEEYLGQISFNNNA
NIVVTDEYGNVIYFTNVLQKGLVTIPKKEDLSHFKNIEKTNTRSVYTSYRKIKFYNSEEKYNLYLTAPLQPVSEASKVLF
LLIPYIGLVVILISVIGGLIYSKVISKPLISMNKVAKEMAKLDFTKKCTVKGEDEIGELSQSLNDLSNNLRISMEELQRA
NEQLLDDIAKEREIEKKRREFIATISHELKTPITILKGQIEGMLSNIGIYKDRDKYLKRNLEVLNDMEYMVKETLEISKL
ESQGFKPRKEQISLSKIVEECIYNISFIAKRKNIFIDKNINEDLFVRGDSKLLKKVVNNIITNAINHSPESEKVYANLHE
EKDEIVLKVENTGIYIEENELKEIFKPFYRIEKSRNRKSGGSGLGLYIVKMILDAHNGKYSISNNEKGVEFKLCLKKYS

Sequences:

>Translated_479_residues
MRNKLKKSIVYKLFLVTTLLLVISSICTYSIIYMLLPNYYHKYKKMTIENQLDSIVDNVPKLDINNIEEYLGQISFNNNA
NIVVTDEYGNVIYFTNVLQKGLVTIPKKEDLSHFKNIEKTNTRSVYTSYRKIKFYNSEEKYNLYLTAPLQPVSEASKVLF
LLIPYIGLVVILISVIGGLIYSKVISKPLISMNKVAKEMAKLDFTKKCTVKGEDEIGELSQSLNDLSNNLRISMEELQRA
NEQLLDDIAKEREIEKKRREFIATISHELKTPITILKGQIEGMLSNIGIYKDRDKYLKRNLEVLNDMEYMVKETLEISKL
ESQGFKPRKEQISLSKIVEECIYNISFIAKRKNIFIDKNINEDLFVRGDSKLLKKVVNNIITNAINHSPESEKVYANLHE
EKDEIVLKVENTGIYIEENELKEIFKPFYRIEKSRNRKSGGSGLGLYIVKMILDAHNGKYSISNNEKGVEFKLCLKKYS
>Mature_479_residues
MRNKLKKSIVYKLFLVTTLLLVISSICTYSIIYMLLPNYYHKYKKMTIENQLDSIVDNVPKLDINNIEEYLGQISFNNNA
NIVVTDEYGNVIYFTNVLQKGLVTIPKKEDLSHFKNIEKTNTRSVYTSYRKIKFYNSEEKYNLYLTAPLQPVSEASKVLF
LLIPYIGLVVILISVIGGLIYSKVISKPLISMNKVAKEMAKLDFTKKCTVKGEDEIGELSQSLNDLSNNLRISMEELQRA
NEQLLDDIAKEREIEKKRREFIATISHELKTPITILKGQIEGMLSNIGIYKDRDKYLKRNLEVLNDMEYMVKETLEISKL
ESQGFKPRKEQISLSKIVEECIYNISFIAKRKNIFIDKNINEDLFVRGDSKLLKKVVNNIITNAINHSPESEKVYANLHE
EKDEIVLKVENTGIYIEENELKEIFKPFYRIEKSRNRKSGGSGLGLYIVKMILDAHNGKYSISNNEKGVEFKLCLKKYS

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=265, Percent_Identity=29.4339622641509, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786783, Length=273, Percent_Identity=27.8388278388278, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=25.830258302583, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87081816, Length=384, Percent_Identity=23.6979166666667, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI145693157, Length=268, Percent_Identity=24.2537313432836, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789149, Length=265, Percent_Identity=27.1698113207547, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788279, Length=264, Percent_Identity=26.8939393939394, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790346, Length=330, Percent_Identity=24.5454545454545, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790551, Length=317, Percent_Identity=25.8675078864353, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1786912, Length=239, Percent_Identity=28.0334728033473, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787894, Length=241, Percent_Identity=26.9709543568465, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788713, Length=245, Percent_Identity=25.3061224489796, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789808, Length=480, Percent_Identity=21.0416666666667, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=27.2321428571429, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87082128, Length=325, Percent_Identity=22.7692307692308, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790861, Length=279, Percent_Identity=22.5806451612903, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=23.5042735042735, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789403, Length=312, Percent_Identity=25.6410256410256, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55392; Mature: 55392

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKLKKSIVYKLFLVTTLLLVISSICTYSIIYMLLPNYYHKYKKMTIENQLDSIVDNVP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KLDINNIEEYLGQISFNNNANIVVTDEYGNVIYFTNVLQKGLVTIPKKEDLSHFKNIEKT
CCCHHHHHHHHHCEEECCCCCEEEEECCCCEEEEHHHHHHCCEECCCHHHHHHHHCCCCC
NTRSVYTSYRKIKFYNSEEKYNLYLTAPLQPVSEASKVLFLLIPYIGLVVILISVIGGLI
CCHHHHHHHHHEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSKVISKPLISMNKVAKEMAKLDFTKKCTVKGEDEIGELSQSLNDLSNNLRISMEELQRA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHH
NEQLLDDIAKEREIEKKRREFIATISHELKTPITILKGQIEGMLSNIGIYKDRDKYLKRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LEVLNDMEYMVKETLEISKLESQGFKPRKEQISLSKIVEECIYNISFIAKRKNIFIDKNI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCC
NEDLFVRGDSKLLKKVVNNIITNAINHSPESEKVYANLHEEKDEIVLKVENTGIYIEENE
CCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEECCEEEEEHHH
LKEIFKPFYRIEKSRNRKSGGSGLGLYIVKMILDAHNGKYSISNNEKGVEFKLCLKKYS
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MRNKLKKSIVYKLFLVTTLLLVISSICTYSIIYMLLPNYYHKYKKMTIENQLDSIVDNVP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KLDINNIEEYLGQISFNNNANIVVTDEYGNVIYFTNVLQKGLVTIPKKEDLSHFKNIEKT
CCCHHHHHHHHHCEEECCCCCEEEEECCCCEEEEHHHHHHCCEECCCHHHHHHHHCCCCC
NTRSVYTSYRKIKFYNSEEKYNLYLTAPLQPVSEASKVLFLLIPYIGLVVILISVIGGLI
CCHHHHHHHHHEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSKVISKPLISMNKVAKEMAKLDFTKKCTVKGEDEIGELSQSLNDLSNNLRISMEELQRA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHH
NEQLLDDIAKEREIEKKRREFIATISHELKTPITILKGQIEGMLSNIGIYKDRDKYLKRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LEVLNDMEYMVKETLEISKLESQGFKPRKEQISLSKIVEECIYNISFIAKRKNIFIDKNI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCC
NEDLFVRGDSKLLKKVVNNIITNAINHSPESEKVYANLHEEKDEIVLKVENTGIYIEENE
CCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEECCEEEEEHHH
LKEIFKPFYRIEKSRNRKSGGSGLGLYIVKMILDAHNGKYSISNNEKGVEFKLCLKKYS
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8631706; 12663927 [H]