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The map label for this gene is gltT [H]

Identifier: 226949261

GI number: 226949261

Start: 2238901

End: 2240196

Strand: Reverse

Name: gltT [H]

Synonym: CLM_2180

Alternate gene names: 226949261

Gene position: 2240196-2238901 (Counterclockwise)

Preceding gene: 226949262

Following gene: 226949260

Centisome position: 53.91

GC content: 42.44

Gene sequence:

>1296_bases
ATGAAATGCTGGAAAGGAGAAAATATGGGGAAGAAGAAAATATGGGAGAGTTATCGATCTCCACTCATTTTGCTTGGGGG
GATTATTGCTGGAGCTATATTGGGTGTTGTTTTGGGAGAAAAGGCAACAGTATTTGAGCCTTTAGGAAATATTTTTATTA
ATTTAATGTTTACTATCGTAGTTCCGATGGTATTTGTTTCAATAGCAACTGCTGTGGGCAGTATGCTGAACATGAAACGT
TTGGGTAAAATTTTGGGGAGTCTGATGTTGACCTTTGTGGTAACCGGATGTTTCGCGGCAGTGCTGGTTCTGGTAGTTGT
CAATATCTGGCCGCCGGCAGCGAATACTGCAATTGCAATGGAAAGCGCAAAGGTGCAGGAGAGTGCAAGTATCAGCTCTA
TGATTGTAGGCTCTTTGACGGTTGACGATTTTTCCGGTCTTATGAGTCGTAAGAACATGCTGCCAATTATTGTATTTGCC
ATCATTTCCGGTCTGGCTGTATCTGCCTGCGGTGGGGAAGAAAGCGCTGTAGGTAAGTTATTAGAAAACCTGAATAGGGT
CATTATGCGGATTGTTGAAATGATTATGAAGTTAGCTCCTATTGGATTGGGCGCTTATTTTGCAAATCTGATTGGTAAAT
TTGGTCCTCAGCTGATTGGGGATTATGGACGTACGATGCTGATTTATTATCCGATGTGCCTTGTTTATGGGATGGTTTTC
TACCCGGCTTATACATATTTTGCTGGAGGAAGGCTGGGAGTAAAGAAAATGCTGCAGAATATTTTGGATCCTGCAGTTAC
TTCATTTGCCACACAAAGCTCTGTGGCTACCTTGCCCGTTAATATGAAAGCCTGTAAAAATATCGGAGTACCGGAGGATA
TCTATAACATCGTTCTCCCTATGGGAGCTACCATGCATATGGACGGTTCTGTAATTTCGTCCATAACGAAGATTGCTTTT
CTGTTCGGTGTGTTCAAAATGCCGTTTACAGGGATGGGAACCTATGCTATGGCTGTCTTGGTATCAATTTTAAGTGCATT
TGTATTATCTGGTGCTCCAGGTGGCGGTTTGGTAGGAGAAATGCTGATTGTCAGTTTCTTTGGGTTTCCAGGGGAAGCGT
TCCCATTAATTGCTACTATCGGATTTTTGGTGGACCCAGCAGCTACCTGCCTCAATTCGTCAGGTGATACTATTGCCTCC
ATGATTATCGCACGTTTTGTGGAAGGCAAGGATTGGCTGTTAAAGGGAGAGATAAATAAAGAGGGGGAGATAAATAAAAT
AGAAATTAGCTGTTAA

Upstream 100 bases:

>100_bases
GAGACTTAATGCAGCATGCCCGCGCTCTGTACTGGAACAGGCATTGAAACAGTTAAAAGAAGCAATCGATAAATTATAAA
ATATAAAAGTTGGGGTTTTT

Downstream 100 bases:

>100_bases
ACAGAGAAAAAAATAAAATAAAAAGAAAGGAAGAAGAATACATGAAAATTATTGAAACAACAAAAGCTCCCGGAGCTATT
GGACCATACTCTCAGGGATA

Product: sodium:dicarboxylate symporter family protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKCWKGENMGKKKIWESYRSPLILLGGIIAGAILGVVLGEKATVFEPLGNIFINLMFTIVVPMVFVSIATAVGSMLNMKR
LGKILGSLMLTFVVTGCFAAVLVLVVVNIWPPAANTAIAMESAKVQESASISSMIVGSLTVDDFSGLMSRKNMLPIIVFA
IISGLAVSACGGEESAVGKLLENLNRVIMRIVEMIMKLAPIGLGAYFANLIGKFGPQLIGDYGRTMLIYYPMCLVYGMVF
YPAYTYFAGGRLGVKKMLQNILDPAVTSFATQSSVATLPVNMKACKNIGVPEDIYNIVLPMGATMHMDGSVISSITKIAF
LFGVFKMPFTGMGTYAMAVLVSILSAFVLSGAPGGGLVGEMLIVSFFGFPGEAFPLIATIGFLVDPAATCLNSSGDTIAS
MIIARFVEGKDWLLKGEINKEGEINKIEISC

Sequences:

>Translated_431_residues
MKCWKGENMGKKKIWESYRSPLILLGGIIAGAILGVVLGEKATVFEPLGNIFINLMFTIVVPMVFVSIATAVGSMLNMKR
LGKILGSLMLTFVVTGCFAAVLVLVVVNIWPPAANTAIAMESAKVQESASISSMIVGSLTVDDFSGLMSRKNMLPIIVFA
IISGLAVSACGGEESAVGKLLENLNRVIMRIVEMIMKLAPIGLGAYFANLIGKFGPQLIGDYGRTMLIYYPMCLVYGMVF
YPAYTYFAGGRLGVKKMLQNILDPAVTSFATQSSVATLPVNMKACKNIGVPEDIYNIVLPMGATMHMDGSVISSITKIAF
LFGVFKMPFTGMGTYAMAVLVSILSAFVLSGAPGGGLVGEMLIVSFFGFPGEAFPLIATIGFLVDPAATCLNSSGDTIAS
MIIARFVEGKDWLLKGEINKEGEINKIEISC
>Mature_431_residues
MKCWKGENMGKKKIWESYRSPLILLGGIIAGAILGVVLGEKATVFEPLGNIFINLMFTIVVPMVFVSIATAVGSMLNMKR
LGKILGSLMLTFVVTGCFAAVLVLVVVNIWPPAANTAIAMESAKVQESASISSMIVGSLTVDDFSGLMSRKNMLPIIVFA
IISGLAVSACGGEESAVGKLLENLNRVIMRIVEMIMKLAPIGLGAYFANLIGKFGPQLIGDYGRTMLIYYPMCLVYGMVF
YPAYTYFAGGRLGVKKMLQNILDPAVTSFATQSSVATLPVNMKACKNIGVPEDIYNIVLPMGATMHMDGSVISSITKIAF
LFGVFKMPFTGMGTYAMAVLVSILSAFVLSGAPGGGLVGEMLIVSFFGFPGEAFPLIATIGFLVDPAATCLNSSGDTIAS
MIIARFVEGKDWLLKGEINKEGEINKIEISC

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI223468564, Length=265, Percent_Identity=28.6792452830189, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI223468566, Length=265, Percent_Identity=28.6792452830189, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI21314632, Length=281, Percent_Identity=27.4021352313167, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI5032093, Length=262, Percent_Identity=29.0076335877863, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI169790839, Length=288, Percent_Identity=26.7361111111111, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4827012, Length=281, Percent_Identity=26.6903914590747, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI40254478, Length=262, Percent_Identity=25.9541984732824, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI66773030, Length=278, Percent_Identity=26.2589928057554, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI194239697, Length=269, Percent_Identity=26.0223048327138, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1790514, Length=393, Percent_Identity=24.1730279898219, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1789947, Length=403, Percent_Identity=23.8213399503722, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789473, Length=253, Percent_Identity=25.296442687747, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788024, Length=420, Percent_Identity=22.8571428571429, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17537407, Length=439, Percent_Identity=25.0569476082005, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=449, Percent_Identity=23.162583518931, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71983106, Length=294, Percent_Identity=25.8503401360544, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983099, Length=294, Percent_Identity=25.8503401360544, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71996953, Length=288, Percent_Identity=26.0416666666667, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI193206654, Length=436, Percent_Identity=19.7247706422018, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17541374, Length=277, Percent_Identity=25.6317689530686, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24583025, Length=401, Percent_Identity=20.6982543640898, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17137668, Length=401, Percent_Identity=20.6982543640898, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583023, Length=401, Percent_Identity=20.6982543640898, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45903; Mature: 45903

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCEEEECC
>Mature Secondary Structure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CCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]