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The map label for this gene is ygfT [C]

Identifier: 226949071

GI number: 226949071

Start: 2049191

End: 2050915

Strand: Direct

Name: ygfT [C]

Synonym: CLM_1987

Alternate gene names: 226949071

Gene position: 2049191-2050915 (Clockwise)

Preceding gene: 226949070

Following gene: 226949072

Centisome position: 49.32

GC content: 28.64

Gene sequence:

>1725_bases
ATGAATTATATAGATATATCCGGCTTAAACTGCAAAAATTGTTATAAATGTTTAAGGGCTTGCCCAGTAAAAGCTATTAA
ATTTAAAAATGAAAAAGCTGAAATAGTTGAAGAAAGATGTATATCCTGTGGAAGATGTCTTGTGATTTGTCCTCAAAATG
CTAAAAAGGATAAACCAGATATAAAATATGTAAAAGATGCTATAAGATCTAGGAAAAAAATTGTGGCTAGTGTTTCCTCA
GTATTTGCAGGAGTATTTAATGTGGAGGAAGGTAAGATAGTTTCTGCTTTAAGAGAATTAGGATTTTCTTACGTAGAGGA
AACAGCCCTTGGTGGAGAAATTGTCACCGAGCTCTATAAGGATTATGTAGAAAGAACTAAATTAAACAATTATATTACCA
CCTCTTGCCCTTCTACTGTAAATTTAATAGAAAAGTATTTTCCTAGTCTTATAAAATATATGATTCCTATTGCTTCTTCC
ATGGCAGCTCATGGAAAAATATTAAAGGAAATTTGGGGTAAGGATGCCTTTGTAGTATTTATAGGCCCTTGTATAGCTAA
AAAGACAGAATCGGATAGTTATAAAAAAGAAAAAATTGTAGATGCTGTTATAAATTTTGATGAGCTGAAGACTTTGTTTA
AGGAGTGTAATATAGAATTAAATTCTATGCCTGTAGGAGAATTTGATAGAAATGCCTACAAAAAAACTCGCTCTTTTACT
TTAAGTGGTGGAATAATAAATTCTATGGGGAATGTAATAGAAAATAATAACTTAATCGCTATGTCAGTAAGTGGGATTGA
AGAATCTATTCAAGTATTAAAAAGTATGGAAATAGGTGAAATATCTAATACCTTTTTAGAAATTACTGCCTGTAGAGGTA
ATTGTGTAGGAGGACCTAACATGATTAAAAATGAAGTTTCTTACTATACAAGATTAAGTAAAGTTAAAAATTATATAAAA
AATAGAGATACTAGTAATACAATAAGATTACTTTCTGTACCGGATAGTATTGATTTTTCAACATCTTTTAAAGATATACA
TTTTGAAAAGAAAAAGGCTCCAAGAAAAGAACTTTTAAAGATAATGAAAAGTATGGGTAAACATACAAAGGAAGATGAAC
TTAATTGTGGAGTATGCGGCTATAACACTTGTATAGAAAAAGCCCAAGCTATTTATGAGGGTATGGCAGAAACTAATATG
TGTCTTCATTTTATGAGAAATAAAGCAGAAAATTTAACTAATGTAATTTTTGAAAATACAGTTAATTGCATCTTTATTTT
AGATGAGAATATGAATGTAAAAGAAATAAATCCTGCTGCTGAAGAAGTTTTTATGATAAAAGCTAAAGATATAAAGGACA
AACCTATATCCCTGTTAATTAATGATGCAGATTTTAGATATGTTAGAGAAACAGGAAAAAGTATTATAGGTAAAAAGGTA
TACTATTATAAATACAATGTAATTTTTATTAAAACTATAGTTTATATGCCAAAACAAGATATAGTTATGGTATCTATGAT
GGATATTACAAAAGAAGAAGAAAATAAAGAAGAACTATTAAAAGTAAAAGAACAAACCATAGATACAGCTCAAAAAGTAA
TAGAAAAGCAAATGAGAGTAGCTCAAGAAATAGCTAGTCTTCTTGGTGAAACTACTGCAGAAACGAAGATTACTTTAACT
AAATTAAAACAAGTAGTGGAAGGAGATAAAAGTGATATAAAATGA

Upstream 100 bases:

>100_bases
CTGAGGGTGTTTCTGTGAAAATTGAAGATAAAATTTATTCTGTAAATGAAGAAAATGTAGAAGAGTTTTTTAATAATGTG
GTATTGAGGGGTATATAGTA

Downstream 100 bases:

>100_bases
ATTTGTTTGTAGAGGTATCCTCTCATAGTTTAAATAAATATGGTGAAGAATTGTGTGGAGACATGGTAGAAGTTGTACGA
CTTTCAGATTGCACTATAAT

Product: sensory box-containing [Fe] hydrogenase

Products: NA

Alternate protein names: CpI; Fe-only hydrogenase; [Fe] hydrogenase [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MNYIDISGLNCKNCYKCLRACPVKAIKFKNEKAEIVEERCISCGRCLVICPQNAKKDKPDIKYVKDAIRSRKKIVASVSS
VFAGVFNVEEGKIVSALRELGFSYVEETALGGEIVTELYKDYVERTKLNNYITTSCPSTVNLIEKYFPSLIKYMIPIASS
MAAHGKILKEIWGKDAFVVFIGPCIAKKTESDSYKKEKIVDAVINFDELKTLFKECNIELNSMPVGEFDRNAYKKTRSFT
LSGGIINSMGNVIENNNLIAMSVSGIEESIQVLKSMEIGEISNTFLEITACRGNCVGGPNMIKNEVSYYTRLSKVKNYIK
NRDTSNTIRLLSVPDSIDFSTSFKDIHFEKKKAPRKELLKIMKSMGKHTKEDELNCGVCGYNTCIEKAQAIYEGMAETNM
CLHFMRNKAENLTNVIFENTVNCIFILDENMNVKEINPAAEEVFMIKAKDIKDKPISLLINDADFRYVRETGKSIIGKKV
YYYKYNVIFIKTIVYMPKQDIVMVSMMDITKEEENKEELLKVKEQTIDTAQKVIEKQMRVAQEIASLLGETTAETKITLT
KLKQVVEGDKSDIK

Sequences:

>Translated_574_residues
MNYIDISGLNCKNCYKCLRACPVKAIKFKNEKAEIVEERCISCGRCLVICPQNAKKDKPDIKYVKDAIRSRKKIVASVSS
VFAGVFNVEEGKIVSALRELGFSYVEETALGGEIVTELYKDYVERTKLNNYITTSCPSTVNLIEKYFPSLIKYMIPIASS
MAAHGKILKEIWGKDAFVVFIGPCIAKKTESDSYKKEKIVDAVINFDELKTLFKECNIELNSMPVGEFDRNAYKKTRSFT
LSGGIINSMGNVIENNNLIAMSVSGIEESIQVLKSMEIGEISNTFLEITACRGNCVGGPNMIKNEVSYYTRLSKVKNYIK
NRDTSNTIRLLSVPDSIDFSTSFKDIHFEKKKAPRKELLKIMKSMGKHTKEDELNCGVCGYNTCIEKAQAIYEGMAETNM
CLHFMRNKAENLTNVIFENTVNCIFILDENMNVKEINPAAEEVFMIKAKDIKDKPISLLINDADFRYVRETGKSIIGKKV
YYYKYNVIFIKTIVYMPKQDIVMVSMMDITKEEENKEELLKVKEQTIDTAQKVIEKQMRVAQEIASLLGETTAETKITLT
KLKQVVEGDKSDIK
>Mature_574_residues
MNYIDISGLNCKNCYKCLRACPVKAIKFKNEKAEIVEERCISCGRCLVICPQNAKKDKPDIKYVKDAIRSRKKIVASVSS
VFAGVFNVEEGKIVSALRELGFSYVEETALGGEIVTELYKDYVERTKLNNYITTSCPSTVNLIEKYFPSLIKYMIPIASS
MAAHGKILKEIWGKDAFVVFIGPCIAKKTESDSYKKEKIVDAVINFDELKTLFKECNIELNSMPVGEFDRNAYKKTRSFT
LSGGIINSMGNVIENNNLIAMSVSGIEESIQVLKSMEIGEISNTFLEITACRGNCVGGPNMIKNEVSYYTRLSKVKNYIK
NRDTSNTIRLLSVPDSIDFSTSFKDIHFEKKKAPRKELLKIMKSMGKHTKEDELNCGVCGYNTCIEKAQAIYEGMAETNM
CLHFMRNKAENLTNVIFENTVNCIFILDENMNVKEINPAAEEVFMIKAKDIKDKPISLLINDADFRYVRETGKSIIGKKV
YYYKYNVIFIKTIVYMPKQDIVMVSMMDITKEEENKEELLKVKEQTIDTAQKVIEKQMRVAQEIASLLGETTAETKITLT
KLKQVVEGDKSDIK

Specific function: Unknown

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR003149
- InterPro:   IPR013352
- InterPro:   IPR001041 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF02256 Fe_hyd_SSU [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 65009; Mature: 65009

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYIDISGLNCKNCYKCLRACPVKAIKFKNEKAEIVEERCISCGRCLVICPQNAKKDKPD
CCEEECCCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCH
IKYVKDAIRSRKKIVASVSSVFAGVFNVEEGKIVSALRELGFSYVEETALGGEIVTELYK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHCCHHHHHHHHH
DYVERTKLNNYITTSCPSTVNLIEKYFPSLIKYMIPIASSMAAHGKILKEIWGKDAFVVF
HHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
IGPCIAKKTESDSYKKEKIVDAVINFDELKTLFKECNIELNSMPVGEFDRNAYKKTRSFT
EECHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCEE
LSGGIINSMGNVIENNNLIAMSVSGIEESIQVLKSMEIGEISNTFLEITACRGNCVGGPN
ECCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCH
MIKNEVSYYTRLSKVKNYIKNRDTSNTIRLLSVPDSIDFSTSFKDIHFEKKKAPRKELLK
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHCHHHCCCHHHHHH
IMKSMGKHTKEDELNCGVCGYNTCIEKAQAIYEGMAETNMCLHFMRNKAENLTNVIFENT
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VNCIFILDENMNVKEINPAAEEVFMIKAKDIKDKPISLLINDADFRYVRETGKSIIGKKV
CCEEEEECCCCCCCCCCCHHHHEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHCCEE
YYYKYNVIFIKTIVYMPKQDIVMVSMMDITKEEENKEELLKVKEQTIDTAQKVIEKQMRV
EEEEEEEEEEEHHHHCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AQEIASLLGETTAETKITLTKLKQVVEGDKSDIK
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNYIDISGLNCKNCYKCLRACPVKAIKFKNEKAEIVEERCISCGRCLVICPQNAKKDKPD
CCEEECCCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCH
IKYVKDAIRSRKKIVASVSSVFAGVFNVEEGKIVSALRELGFSYVEETALGGEIVTELYK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHCCHHHHHHHHH
DYVERTKLNNYITTSCPSTVNLIEKYFPSLIKYMIPIASSMAAHGKILKEIWGKDAFVVF
HHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
IGPCIAKKTESDSYKKEKIVDAVINFDELKTLFKECNIELNSMPVGEFDRNAYKKTRSFT
EECHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCEE
LSGGIINSMGNVIENNNLIAMSVSGIEESIQVLKSMEIGEISNTFLEITACRGNCVGGPN
ECCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCH
MIKNEVSYYTRLSKVKNYIKNRDTSNTIRLLSVPDSIDFSTSFKDIHFEKKKAPRKELLK
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHCHHHCCCHHHHHH
IMKSMGKHTKEDELNCGVCGYNTCIEKAQAIYEGMAETNMCLHFMRNKAENLTNVIFENT
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VNCIFILDENMNVKEINPAAEEVFMIKAKDIKDKPISLLINDADFRYVRETGKSIIGKKV
CCEEEEECCCCCCCCCCCHHHHEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHCCEE
YYYKYNVIFIKTIVYMPKQDIVMVSMMDITKEEENKEELLKVKEQTIDTAQKVIEKQMRV
EEEEEEEEEEEHHHHCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AQEIASLLGETTAETKITLTKLKQVVEGDKSDIK
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1911757; 9836629; 10529166 [H]