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The map label for this gene is nirB [C]

Identifier: 226949020

GI number: 226949020

Start: 1985048

End: 1985902

Strand: Direct

Name: nirB [C]

Synonym: CLM_1934

Alternate gene names: 226949020

Gene position: 1985048-1985902 (Clockwise)

Preceding gene: 226949018

Following gene: 226949022

Centisome position: 47.77

GC content: 32.05

Gene sequence:

>855_bases
ATGAAAATGATAAGTAAAAATGAAATTGCAAAACTTAAAGGTGAAGGATTTTTAGCTCAAAAACAAGAAGGATATTTTTC
TGTAAGAATATTAAGTAGAGCGGGGAATTTTACTTCTAAACAGATTATAAAATTGGCAGAAATAGCAGATAATTATGGGA
AAGGCTATTTAGGAGTGACTACAAGATTAGCTACAGAAATTCCTTGGATTAAATACGAAGATATAGAAGCAGTAAAAAAA
GAAATAGTATCTAGTGGTATGGGCCATGGAGGTACAGGTAAAAAAGTGAGACCTCTAGTATCTTGTAAGGGTACCGTTTG
TGTTCATGGATTATATGATACACAAAAACTTTGTGGTGAGCTTCATGATAAATACTTTGGATATGAACTACCATCAAAAT
TTAAAATAACTTTAGTGGGATGTCCTAATAACTGTGCTAAGGCATCTATTAATGATATAGGAATTATGGGACAGGCATAT
GTGGAATTCAATGAAGAAAAATGTAAAGCTTGTGGTATATGTACCAAATCCTGTAGGCAAAAGGCTGTTACTGTAGAAAA
TAACAAAATAGTATATAAAAAGGACTTATGTGTAAATTGTGGTAAATGTGCTACTGTTTGTCCTTTTGAAGCTATGACCA
TTAAAGAGCAAGGATTACAAGTATATTTAGGTGGAAGATTTGGAAGGGAATATAGAATAGGTAATAGGTTAAAGGGGTTA
TATAAGGAAGAAGAAATACCAGCTTTAGTACAAAAGATTTTTGATGTATATAGTGAAATGGGAAATCCCGGTGAAAGATT
AGCTAAAATGATAGAAAGAGTAGGCTTTGATAAGGTGGAAGAAGCCATATTATAA

Upstream 100 bases:

>100_bases
CTATTTTATATTTATTTTAGTACTAAATTAATAAAAATCTATATTTAAAAATAATTTTAAAGCTGAAATAACTATAAAAA
GGCTAGTTTAAAGTCAACTA

Downstream 100 bases:

>100_bases
TAAAAATAAGCAAGGGAATGTAATATATAGTGAACCCTTGCTTATTTCAGTTTTATATGAAACTCCTGTTCCTTTATCAC
AGGAGTAAGCGACTCACACC

Product: sulfite/nitrite reductase family protein

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKMISKNEIAKLKGEGFLAQKQEGYFSVRILSRAGNFTSKQIIKLAEIADNYGKGYLGVTTRLATEIPWIKYEDIEAVKK
EIVSSGMGHGGTGKKVRPLVSCKGTVCVHGLYDTQKLCGELHDKYFGYELPSKFKITLVGCPNNCAKASINDIGIMGQAY
VEFNEEKCKACGICTKSCRQKAVTVENNKIVYKKDLCVNCGKCATVCPFEAMTIKEQGLQVYLGGRFGREYRIGNRLKGL
YKEEEIPALVQKIFDVYSEMGNPGERLAKMIERVGFDKVEEAIL

Sequences:

>Translated_284_residues
MKMISKNEIAKLKGEGFLAQKQEGYFSVRILSRAGNFTSKQIIKLAEIADNYGKGYLGVTTRLATEIPWIKYEDIEAVKK
EIVSSGMGHGGTGKKVRPLVSCKGTVCVHGLYDTQKLCGELHDKYFGYELPSKFKITLVGCPNNCAKASINDIGIMGQAY
VEFNEEKCKACGICTKSCRQKAVTVENNKIVYKKDLCVNCGKCATVCPFEAMTIKEQGLQVYLGGRFGREYRIGNRLKGL
YKEEEIPALVQKIFDVYSEMGNPGERLAKMIERVGFDKVEEAIL
>Mature_284_residues
MKMISKNEIAKLKGEGFLAQKQEGYFSVRILSRAGNFTSKQIIKLAEIADNYGKGYLGVTTRLATEIPWIKYEDIEAVKK
EIVSSGMGHGGTGKKVRPLVSCKGTVCVHGLYDTQKLCGELHDKYFGYELPSKFKITLVGCPNNCAKASINDIGIMGQAY
VEFNEEKCKACGICTKSCRQKAVTVENNKIVYKKDLCVNCGKCATVCPFEAMTIKEQGLQVYLGGRFGREYRIGNRLKGL
YKEEEIPALVQKIFDVYSEMGNPGERLAKMIERVGFDKVEEAIL

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG2221

COG function: function code C; Dissimilatory sulfite reductase (desulfoviridin), alpha and beta subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR007516
- InterPro:   IPR007525
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF00037 Fer4; PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 31593; Mature: 31593

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00365 NIR_SIR ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMISKNEIAKLKGEGFLAQKQEGYFSVRILSRAGNFTSKQIIKLAEIADNYGKGYLGVT
CCCCCCCCHHHHCCCCCEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEHHH
TRLATEIPWIKYEDIEAVKKEIVSSGMGHGGTGKKVRPLVSCKGTVCVHGLYDTQKLCGE
HHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEEECCHHHHHHHHH
LHDKYFGYELPSKFKITLVGCPNNCAKASINDIGIMGQAYVEFNEEKCKACGICTKSCRQ
HHHHHCCCCCCCCEEEEEEECCCCHHHCCCCHHCCCCCEEEEECHHHHHHHHHHHHHHHC
KAVTVENNKIVYKKDLCVNCGKCATVCPFEAMTIKEQGLQVYLGGRFGREYRIGNRLKGL
CCEEEECCEEEEECCCCCCCCCCCEECCCHHHEEHHCCCEEEECCCCCCCCCCCHHHHHH
YKEEEIPALVQKIFDVYSEMGNPGERLAKMIERVGFDKVEEAIL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCC
>Mature Secondary Structure
MKMISKNEIAKLKGEGFLAQKQEGYFSVRILSRAGNFTSKQIIKLAEIADNYGKGYLGVT
CCCCCCCCHHHHCCCCCEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEHHH
TRLATEIPWIKYEDIEAVKKEIVSSGMGHGGTGKKVRPLVSCKGTVCVHGLYDTQKLCGE
HHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEEECCHHHHHHHHH
LHDKYFGYELPSKFKITLVGCPNNCAKASINDIGIMGQAYVEFNEEKCKACGICTKSCRQ
HHHHHCCCCCCCCEEEEEEECCCCHHHCCCCHHCCCCCEEEEECHHHHHHHHHHHHHHHC
KAVTVENNKIVYKKDLCVNCGKCATVCPFEAMTIKEQGLQVYLGGRFGREYRIGNRLKGL
CCEEEECCEEEEECCCCCCCCCCCEECCCHHHEEHHCCCEEEECCCCCCCCCCCHHHHHH
YKEEEIPALVQKIFDVYSEMGNPGERLAKMIERVGFDKVEEAIL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]