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The map label for this gene is psuK [H]

Identifier: 226949015

GI number: 226949015

Start: 1977769

End: 1978728

Strand: Direct

Name: psuK [H]

Synonym: CLM_1929

Alternate gene names: 226949015

Gene position: 1977769-1978728 (Clockwise)

Preceding gene: 226949014

Following gene: 226949018

Centisome position: 47.6

GC content: 28.12

Gene sequence:

>960_bases
ATGAATTACGATTGTAATAATAAACCATATGTTTTAGTTTTTGGAGCATCAGTAGTAGATATTTTTGGTTTTAGTTGTTG
TGACTATAGAGCTTACAATTCTAATCCGGGTAAAATAAAAATATCCTTTGGAGGGGTATGTAGAAATATAGCAGAAAATT
TATCAAGAGTTGGAATTAATACTAAATTTATATCTATATTAGGTGAGGATGAGAATGCTAAGTGTATGTTAGAACATTCA
AGAAAAATGGGATATGACATGGGAGACTCTCTTATTTTAAAAGGAGGTACAACCCCTACTTACATGGCTATATTAGATGA
AAATGGAGAAATGGTTTCAGCAGTGGCTGATATGAAAAGTATAGGCGAAATGAATTCCCAATTTATAGATTCAAAGGCGG
ATATAATAAAAAAGGCAGAGTATACAGTCTTGGATTCAGATAATCCTGAAATATTAGAATATATATTAAAAAAGTTCAAA
GGAAAAACAAAATTTATATTGGATCCTGTTTCTGCTGAAAAAGCTAAAAAAATAAAGCCATTAATAAAATATTTTCATAC
TATTAAACCTAATATACATGAAGCAGAAGTTTTATCAGGTTTCAAAATAGAAACTATAGAAGATTTACAGAAAGCAGGAG
AGTATTTTAAATCTTTAGGTGTGGAAAATGTTTTTATAAGTTTAGATGCAGAAGGGATTTATTATAAAAATAAACTTGAA
GAGGGAAGAATAAATGCTAATGATGTTACAGTTAAAAATGTTACAGGAGCAGGAGATGCTTTTGTAGCAGGTATAGCATA
TGGTTATATGAATAAATTATCTTTAGAGAAAACTGTAAAGTTTGCTATAACTATGGCTAATTTAACTATAGCTCACGAAA
ACACTATAAATCCAGATTTAAGTTATAATATTGTAGAGAATAATATAAAAAATATTCAATGGATTGAAGAATACTTATAA

Upstream 100 bases:

>100_bases
AAAAAAGAATATTGGAGGAAGTAGAAAAGCTATTAGAAATAAAAAAACTGGTGGATTATTTGATAAATAGATAATATTTA
TATTGTGGAGGTTACTAAGG

Downstream 100 bases:

>100_bases
TTAAATCAGGTAATATAGGTAATTATAAGTATAAGAAATATAATACATAAAATGATTACGTAACAAAGTACTAAAAATGA
ATTTCATTTTAACAATAAAA

Product: PfkB family kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MNYDCNNKPYVLVFGASVVDIFGFSCCDYRAYNSNPGKIKISFGGVCRNIAENLSRVGINTKFISILGEDENAKCMLEHS
RKMGYDMGDSLILKGGTTPTYMAILDENGEMVSAVADMKSIGEMNSQFIDSKADIIKKAEYTVLDSDNPEILEYILKKFK
GKTKFILDPVSAEKAKKIKPLIKYFHTIKPNIHEAEVLSGFKIETIEDLQKAGEYFKSLGVENVFISLDAEGIYYKNKLE
EGRINANDVTVKNVTGAGDAFVAGIAYGYMNKLSLEKTVKFAITMANLTIAHENTINPDLSYNIVENNIKNIQWIEEYL

Sequences:

>Translated_319_residues
MNYDCNNKPYVLVFGASVVDIFGFSCCDYRAYNSNPGKIKISFGGVCRNIAENLSRVGINTKFISILGEDENAKCMLEHS
RKMGYDMGDSLILKGGTTPTYMAILDENGEMVSAVADMKSIGEMNSQFIDSKADIIKKAEYTVLDSDNPEILEYILKKFK
GKTKFILDPVSAEKAKKIKPLIKYFHTIKPNIHEAEVLSGFKIETIEDLQKAGEYFKSLGVENVFISLDAEGIYYKNKLE
EGRINANDVTVKNVTGAGDAFVAGIAYGYMNKLSLEKTVKFAITMANLTIAHENTINPDLSYNIVENNIKNIQWIEEYL
>Mature_319_residues
MNYDCNNKPYVLVFGASVVDIFGFSCCDYRAYNSNPGKIKISFGGVCRNIAENLSRVGINTKFISILGEDENAKCMLEHS
RKMGYDMGDSLILKGGTTPTYMAILDENGEMVSAVADMKSIGEMNSQFIDSKADIIKKAEYTVLDSDNPEILEYILKKFK
GKTKFILDPVSAEKAKKIKPLIKYFHTIKPNIHEAEVLSGFKIETIEDLQKAGEYFKSLGVENVFISLDAEGIYYKNKLE
EGRINANDVTVKNVTGAGDAFVAGIAYGYMNKLSLEKTVKFAITMANLTIAHENTINPDLSYNIVENNIKNIQWIEEYL

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788491, Length=306, Percent_Identity=34.3137254901961, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1788484, Length=301, Percent_Identity=30.5647840531561, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1790193, Length=226, Percent_Identity=24.7787610619469, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788493, Length=126, Percent_Identity=30.952380952381, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87081971, Length=324, Percent_Identity=24.0740740740741, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25147470, Length=329, Percent_Identity=25.531914893617, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 35615; Mature: 35615

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYDCNNKPYVLVFGASVVDIFGFSCCDYRAYNSNPGKIKISFGGVCRNIAENLSRVGIN
CCCCCCCCCEEEEECCHHHHHHCCCCCCCEEECCCCCEEEEEECHHHHHHHHHHHHCCCC
TKFISILGEDENAKCMLEHSRKMGYDMGDSLILKGGTTPTYMAILDENGEMVSAVADMKS
EEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHH
IGEMNSQFIDSKADIIKKAEYTVLDSDNPEILEYILKKFKGKTKFILDPVSAEKAKKIKP
HHHHHHHHHCCHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
LIKYFHTIKPNIHEAEVLSGFKIETIEDLQKAGEYFKSLGVENVFISLDAEGIYYKNKLE
HHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCC
EGRINANDVTVKNVTGAGDAFVAGIAYGYMNKLSLEKTVKFAITMANLTIAHENTINPDL
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCCCCCC
SYNIVENNIKNIQWIEEYL
EEEEHHCCCCHHHHHHHCC
>Mature Secondary Structure
MNYDCNNKPYVLVFGASVVDIFGFSCCDYRAYNSNPGKIKISFGGVCRNIAENLSRVGIN
CCCCCCCCCEEEEECCHHHHHHCCCCCCCEEECCCCCEEEEEECHHHHHHHHHHHHCCCC
TKFISILGEDENAKCMLEHSRKMGYDMGDSLILKGGTTPTYMAILDENGEMVSAVADMKS
EEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHH
IGEMNSQFIDSKADIIKKAEYTVLDSDNPEILEYILKKFKGKTKFILDPVSAEKAKKIKP
HHHHHHHHHCCHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
LIKYFHTIKPNIHEAEVLSGFKIETIEDLQKAGEYFKSLGVENVFISLDAEGIYYKNKLE
HHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCC
EGRINANDVTVKNVTGAGDAFVAGIAYGYMNKLSLEKTVKFAITMANLTIAHENTINPDL
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCCCCCC
SYNIVENNIKNIQWIEEYL
EEEEHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]