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The map label for this gene is vanS [H]

Identifier: 226948940

GI number: 226948940

Start: 1888718

End: 1890820

Strand: Reverse

Name: vanS [H]

Synonym: CLM_1853

Alternate gene names: 226948940

Gene position: 1890820-1888718 (Counterclockwise)

Preceding gene: 226948941

Following gene: 226948937

Centisome position: 45.5

GC content: 23.68

Gene sequence:

>2103_bases
ATGCTGTCTCTTTCATTTTTAACCGGCTATGATTTAATAAAAAACAAAAGGTATCTTGTGAAAGCCCCTTATTTTAATAA
CCCTGAATTTGACATGGAAATATATTCTTATTGTTCAAACTTGTATAACTTTCATATAACCTATAAAAATTTTGATTATA
AAGTAGCAGAAAATAAGGTTACTAAAGAACAATTAGCAGACTTAAAACTATTTTATGAAGATATGATAAGAAATTCTCAA
GAAGATATTGGAAATAAGTATATTAGTATTTTATCAACAGCTGCACAATCTGATGATAAAGATAAATTTACTAAGTTAAC
ACAAGAAAAAAATAAAGAACTTAAAGAAGTAGAAAAAGAAAATACTAAAACTGAGGCTGAATTAAAAAAAGAAATTGCAT
TATGGTCTTACAGTGACTACAAAAATATAAAAAAAGCTATAGAATCTAAAAAAGAGATAAAATACTATATTAAAAACAGC
TTAACCAAAGAAGTTTATACTAATTTAGCACCTAAAACTAATATAGATAGTTATATAAAAAATAATTCTATTTATTCTAT
AAGTTTTCCATTAAAATCTTATAATACTAAAAATTTTTTAGAAACCAATAATTTACTTAATTCTTTTAACTGGGAAGGAA
ATATTATAATTACAAAGGATTTTAATTCTGATGGTTATATATTTAAAAATTATAATTATTATAATTCCATTAGAGATAGG
CTTGTAAAAGAAATAATCATAGGCGTTCCCTCTTTAATTATGGGAATTTTTATGTTAATTATTTTTAAAAGAAGAAATTA
TTTAAATTCTCCTATTTTAAATAAAGTAAAAGAAATGTATAATAATATACCCTTAGATTTAAAACTATTCATATTTGTTT
TATATACCGCCATAATAGGGAGTTATTTAATAAATCTTAGTTTTTTTTATAAACCCTTAGGAATTAATCACTTTGTTATT
ATTACTTTAGTAAGTCTTTATATTCTATATTTAATATTTAATGTAAAATATGCTATAGGACTAAAGAAAGATAAAAAGCA
GTTTTTAATTCAATGTAAGGGAAGTTTCATTAATAAATTATTGAATGCTTTTAAAAATAGTAATTTGAATAAAAATATTA
AATTAAAAATAGCTTTTATTATGGCATTATCTTCTATAGTAGCTGCATTTGTACTTTTAGTATTGCTACTAGGAATTCAT
AATATAATAGTTGCATCTATTGTAGCTATTATTTATATAGTTTTGCTTATTAGATACTTATTTAAAAAGGCTGATTATTT
AAATGAAATATTAAAAGCTACAGAAGAAATGTCCTATGGAAATTTAGATTATGTTATAAAGGAAAATGGAGAAAGCGCTC
TAGATAAAATAGCCCATAATATCAATAATATGAAAGATGGTTATAAAAAATCTCTAGAAGAGCAGGTTAAAAGTGAAAGA
TTAAAAACTGAACTTATAACCAATGTATCTCATGATTTAAAAACTCCCCTTACCTCTATAATAAATTATATAGATTTACT
TAAAAAAGAGGATTTAAGTAAAGATGAAATTAATGGATATATTTCTGTTCTAGATAGAAAGTCTAAAAGACTTAAATCAC
TTATAGAGGATTTATTTGAGGCATCAAAAATGTCCAGCGGTGCAGTAGAATTAAATATTGAAAAAATAAATGTAACAGCT
TTATTAAAGCAATCCATAGCAGAATTTGAAGAAAAAATCACGAAATCCTCTCTAGAACTTAAATTTAAATATGATAATAA
TAAAACCTATGCAAATTTAGATGGAAAGAAAACCTGGAGAGTATTTGAAAATTTAATAAACAATATTATAAAGTATTCTC
AACCTAATACTAGAGTATATATAGATTTAATAGAAACAAATACTAAAATAATAATTACTATGAAAAATATCTCACGCTAT
GAAATGGATTTTAGTGCTGATGAAATTTTTGAAAGATTTAAAAGGGGAGATAAGGCTAGAAACACAGATGGATCTGGGCT
AGGTCTTGCTATTGCAAAAAGTATTTCAGAACTTCAGGGTGGGAGCTTAAACATTATCATTGATGGGGATCTTTTTAAGG
TTATTGTAGAGTTTAACAAATAA

Upstream 100 bases:

>100_bases
AACTCTAGAGAACCAAGATATTTAAAGGTGGTGTGGGGAATTGGTTATAAAATCCAAGAATAAATTTATTTATATAATAT
GTTTCATTGTAGGTATATAT

Downstream 100 bases:

>100_bases
TATATTTAACTTTGATTCCACTAATATAAAAAATAAGATTATTTATATATTATTCTAATATTTTATAACTATTCTCAAAA
ATATATAATTTATAACTTTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 700; Mature: 700

Protein sequence:

>700_residues
MLSLSFLTGYDLIKNKRYLVKAPYFNNPEFDMEIYSYCSNLYNFHITYKNFDYKVAENKVTKEQLADLKLFYEDMIRNSQ
EDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKENTKTEAELKKEIALWSYSDYKNIKKAIESKKEIKYYIKNS
LTKEVYTNLAPKTNIDSYIKNNSIYSISFPLKSYNTKNFLETNNLLNSFNWEGNIIITKDFNSDGYIFKNYNYYNSIRDR
LVKEIIIGVPSLIMGIFMLIIFKRRNYLNSPILNKVKEMYNNIPLDLKLFIFVLYTAIIGSYLINLSFFYKPLGINHFVI
ITLVSLYILYLIFNVKYAIGLKKDKKQFLIQCKGSFINKLLNAFKNSNLNKNIKLKIAFIMALSSIVAAFVLLVLLLGIH
NIIVASIVAIIYIVLLIRYLFKKADYLNEILKATEEMSYGNLDYVIKENGESALDKIAHNINNMKDGYKKSLEEQVKSER
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKSLIEDLFEASKMSSGAVELNIEKINVTA
LLKQSIAEFEEKITKSSLELKFKYDNNKTYANLDGKKTWRVFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNISRY
EMDFSADEIFERFKRGDKARNTDGSGLGLAIAKSISELQGGSLNIIIDGDLFKVIVEFNK

Sequences:

>Translated_700_residues
MLSLSFLTGYDLIKNKRYLVKAPYFNNPEFDMEIYSYCSNLYNFHITYKNFDYKVAENKVTKEQLADLKLFYEDMIRNSQ
EDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKENTKTEAELKKEIALWSYSDYKNIKKAIESKKEIKYYIKNS
LTKEVYTNLAPKTNIDSYIKNNSIYSISFPLKSYNTKNFLETNNLLNSFNWEGNIIITKDFNSDGYIFKNYNYYNSIRDR
LVKEIIIGVPSLIMGIFMLIIFKRRNYLNSPILNKVKEMYNNIPLDLKLFIFVLYTAIIGSYLINLSFFYKPLGINHFVI
ITLVSLYILYLIFNVKYAIGLKKDKKQFLIQCKGSFINKLLNAFKNSNLNKNIKLKIAFIMALSSIVAAFVLLVLLLGIH
NIIVASIVAIIYIVLLIRYLFKKADYLNEILKATEEMSYGNLDYVIKENGESALDKIAHNINNMKDGYKKSLEEQVKSER
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKSLIEDLFEASKMSSGAVELNIEKINVTA
LLKQSIAEFEEKITKSSLELKFKYDNNKTYANLDGKKTWRVFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNISRY
EMDFSADEIFERFKRGDKARNTDGSGLGLAIAKSISELQGGSLNIIIDGDLFKVIVEFNK
>Mature_700_residues
MLSLSFLTGYDLIKNKRYLVKAPYFNNPEFDMEIYSYCSNLYNFHITYKNFDYKVAENKVTKEQLADLKLFYEDMIRNSQ
EDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKENTKTEAELKKEIALWSYSDYKNIKKAIESKKEIKYYIKNS
LTKEVYTNLAPKTNIDSYIKNNSIYSISFPLKSYNTKNFLETNNLLNSFNWEGNIIITKDFNSDGYIFKNYNYYNSIRDR
LVKEIIIGVPSLIMGIFMLIIFKRRNYLNSPILNKVKEMYNNIPLDLKLFIFVLYTAIIGSYLINLSFFYKPLGINHFVI
ITLVSLYILYLIFNVKYAIGLKKDKKQFLIQCKGSFINKLLNAFKNSNLNKNIKLKIAFIMALSSIVAAFVLLVLLLGIH
NIIVASIVAIIYIVLLIRYLFKKADYLNEILKATEEMSYGNLDYVIKENGESALDKIAHNINNMKDGYKKSLEEQVKSER
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKSLIEDLFEASKMSSGAVELNIEKINVTA
LLKQSIAEFEEKITKSSLELKFKYDNNKTYANLDGKKTWRVFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNISRY
EMDFSADEIFERFKRGDKARNTDGSGLGLAIAKSISELQGGSLNIIIDGDLFKVIVEFNK

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=256, Percent_Identity=23.4375, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787894, Length=285, Percent_Identity=24.5614035087719, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1788713, Length=211, Percent_Identity=24.1706161137441, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1786600, Length=411, Percent_Identity=22.1411192214112, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=265, Percent_Identity=24.1509433962264, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=24.1525423728814, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788279, Length=292, Percent_Identity=26.027397260274, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790346, Length=276, Percent_Identity=25.3623188405797, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082128, Length=301, Percent_Identity=26.578073089701, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI145693157, Length=232, Percent_Identity=23.2758620689655, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 81153; Mature: 81153

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLSFLTGYDLIKNKRYLVKAPYFNNPEFDMEIYSYCSNLYNFHITYKNFDYKVAENKV
CCEEHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHEEEEEEECCCEEECCCHH
TKEQLADLKLFYEDMIRNSQEDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NTKTEAELKKEIALWSYSDYKNIKKAIESKKEIKYYIKNSLTKEVYTNLAPKTNIDSYIK
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHC
NNSIYSISFPLKSYNTKNFLETNNLLNSFNWEGNIIITKDFNSDGYIFKNYNYYNSIRDR
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHH
LVKEIIIGVPSLIMGIFMLIIFKRRNYLNSPILNKVKEMYNNIPLDLKLFIFVLYTAIIG
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SYLINLSFFYKPLGINHFVIITLVSLYILYLIFNVKYAIGLKKDKKQFLIQCKGSFINKL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LNAFKNSNLNKNIKLKIAFIMALSSIVAAFVLLVLLLGIHNIIVASIVAIIYIVLLIRYL
HHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKADYLNEILKATEEMSYGNLDYVIKENGESALDKIAHNINNMKDGYKKSLEEQVKSER
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKSLIEDLFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASKMSSGAVELNIEKINVTALLKQSIAEFEEKITKSSLELKFKYDNNKTYANLDGKKTWR
HHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCCHHHHH
VFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNISRYEMDFSADEIFERFKRGDKAR
HHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEECCCHHCCCCCHHHHHHHHHCCCCCC
NTDGSGLGLAIAKSISELQGGSLNIIIDGDLFKVIVEFNK
CCCCCCHHHHHHHHHHHHCCCCEEEEECCHHEEHHEECCC
>Mature Secondary Structure
MLSLSFLTGYDLIKNKRYLVKAPYFNNPEFDMEIYSYCSNLYNFHITYKNFDYKVAENKV
CCEEHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHEEEEEEECCCEEECCCHH
TKEQLADLKLFYEDMIRNSQEDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NTKTEAELKKEIALWSYSDYKNIKKAIESKKEIKYYIKNSLTKEVYTNLAPKTNIDSYIK
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHC
NNSIYSISFPLKSYNTKNFLETNNLLNSFNWEGNIIITKDFNSDGYIFKNYNYYNSIRDR
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHH
LVKEIIIGVPSLIMGIFMLIIFKRRNYLNSPILNKVKEMYNNIPLDLKLFIFVLYTAIIG
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SYLINLSFFYKPLGINHFVIITLVSLYILYLIFNVKYAIGLKKDKKQFLIQCKGSFINKL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LNAFKNSNLNKNIKLKIAFIMALSSIVAAFVLLVLLLGIHNIIVASIVAIIYIVLLIRYL
HHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKADYLNEILKATEEMSYGNLDYVIKENGESALDKIAHNINNMKDGYKKSLEEQVKSER
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKSLIEDLFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASKMSSGAVELNIEKINVTALLKQSIAEFEEKITKSSLELKFKYDNNKTYANLDGKKTWR
HHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCCHHHHH
VFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNISRYEMDFSADEIFERFKRGDKAR
HHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEECCCHHCCCCCHHHHHHHHHCCCCCC
NTDGSGLGLAIAKSISELQGGSLNIIIDGDLFKVIVEFNK
CCCCCCHHHHHHHHHHHHCCCCEEEEECCHHEEHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]