| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is ybbF [H]
Identifier: 226948626
GI number: 226948626
Start: 1578562
End: 1579917
Strand: Direct
Name: ybbF [H]
Synonym: CLM_1521
Alternate gene names: 226948626
Gene position: 1578562-1579917 (Clockwise)
Preceding gene: 226948625
Following gene: 226948627
Centisome position: 37.99
GC content: 34.14
Gene sequence:
>1356_bases ATGTCAAATGTAAAAGATATAGCTCAAAGTATATATGACATAATTGGTGGTAGTAGCAATATAGTTTCATTTACGCATTG TATGACAAGATTGAGACTTGTGCTTAAGGATCAAAATAATCTAGAGATGAAGGCTATAAAAAAAATTGATGGAGTTATGG GCGTAGTACAGCAACAAAATCAACTTCAAATAATTCTTGGACCAGGTAGAGTAAATAAAGTTACAGAGGAATTTGAAAAG ATTTGTGGAATTCAGACAGAAGAAGTAACAAAAGAGGTTGAGTTTGGAGATGGAAGTAAGTTAAGAGAAGAAATAAAGGA AAAGAATGCAACGCCATTTAAATTATTTTTAAAGAAATTATCTAATATATTCATACCATTAATTCCAGCATTTATAGCTT GTGGATTAGTTACTGCAATATTAAATATAGTGCTTAAATTAGATCCAGCATTTGCAGAAACAACAATTGGAGGATTATTA AAAATAGCAGGTAATGCAGTGTTTTGGGGGTTAAATCTATTTGTAGGTGTTAATGCAGCTAAAGAATTCGGTGGTTCTCC AATGCTTGGAGGTACTATGGCGGCTATAATATCTCATCCAGAATTAGCTAAGGTAACATTGAGTGGTCAAAAGCTTTTAC CTGGTAGAGGCGGTATTATTGCAGTATTATTAGTTGTTGCATTTTCAAGTTTCTTAGAAAAGAAAGTAAGAAAAATAATG CCAGATGCATTAGATTTATTTATGACTCCATTAATAGTAATATTAGTATCAACTTTTGTTTCAATATTTGCATTGCAACC AGCTGGTGGATTTATTTCAGATGCTATAGGTAATGGAACTAAAGCAGCAATTTCAGGTGGTGGAGCCGTAACAGGATTTA TATTAGGAGGATTCTTCCTACCATTAGTAATGACAGGATTACATCAAGGATTAACACCAATACATGCGGATCTATTAAAA ACTACAGGGTTAAATCCACTTCTTCCAATACTAGCTATGGCAGGAGCAGGACAAGTAGGAGCAGCTATAGCAGTTTTACT CAGAACCAAAAATAAAAGATTAAAAAAGACAATAATCTCAGCATTACCAGTGGGAATTATGGGAGTTGGAGAACCCCTTA TATATGGTGTAACATTGCCGTTAGGAAAGCCATTTATAGGAGCATGTATAGGAGGAGCAGTAGGTGGTGCAGTTCAAGCT TTGTTTAAAGTCTCTTCAACATCAATGGGCTTATCTGGATTACCATTAGCTGCAATAACTAGTACACCATTGTTGTTCAT TATAGGCTTGGGAGCAGCTTATATAGGTGGATTTTTTGCAACATTACTTTTAGGATTTGAAGATCCAATAGAATAA
Upstream 100 bases:
>100_bases TAGATAAAAATAGAATAATTATAGGGTGTAATTTAGAAATAAGATTATTTTTTAAATTTAAAACGCTCAGTTATATTTAA AAATAAAGGGGGATTTTATT
Downstream 100 bases:
>100_bases TCTTTAAAATATATATAAAGCTACAGCTAAATATCATTAATATACTAATAAGTAAGGTATAAAAGGAATAGACGAAATAT TGGGAAAAAACAAAATAAAA
Product: PTS system transporter subunit IIBC
Products: NA
Alternate protein names: Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]
Number of amino acids: Translated: 451; Mature: 450
Protein sequence:
>451_residues MSNVKDIAQSIYDIIGGSSNIVSFTHCMTRLRLVLKDQNNLEMKAIKKIDGVMGVVQQQNQLQIILGPGRVNKVTEEFEK ICGIQTEEVTKEVEFGDGSKLREEIKEKNATPFKLFLKKLSNIFIPLIPAFIACGLVTAILNIVLKLDPAFAETTIGGLL KIAGNAVFWGLNLFVGVNAAKEFGGSPMLGGTMAAIISHPELAKVTLSGQKLLPGRGGIIAVLLVVAFSSFLEKKVRKIM PDALDLFMTPLIVILVSTFVSIFALQPAGGFISDAIGNGTKAAISGGGAVTGFILGGFFLPLVMTGLHQGLTPIHADLLK TTGLNPLLPILAMAGAGQVGAAIAVLLRTKNKRLKKTIISALPVGIMGVGEPLIYGVTLPLGKPFIGACIGGAVGGAVQA LFKVSSTSMGLSGLPLAAITSTPLLFIIGLGAAYIGGFFATLLLGFEDPIE
Sequences:
>Translated_451_residues MSNVKDIAQSIYDIIGGSSNIVSFTHCMTRLRLVLKDQNNLEMKAIKKIDGVMGVVQQQNQLQIILGPGRVNKVTEEFEK ICGIQTEEVTKEVEFGDGSKLREEIKEKNATPFKLFLKKLSNIFIPLIPAFIACGLVTAILNIVLKLDPAFAETTIGGLL KIAGNAVFWGLNLFVGVNAAKEFGGSPMLGGTMAAIISHPELAKVTLSGQKLLPGRGGIIAVLLVVAFSSFLEKKVRKIM PDALDLFMTPLIVILVSTFVSIFALQPAGGFISDAIGNGTKAAISGGGAVTGFILGGFFLPLVMTGLHQGLTPIHADLLK TTGLNPLLPILAMAGAGQVGAAIAVLLRTKNKRLKKTIISALPVGIMGVGEPLIYGVTLPLGKPFIGACIGGAVGGAVQA LFKVSSTSMGLSGLPLAAITSTPLLFIIGLGAAYIGGFFATLLLGFEDPIE >Mature_450_residues SNVKDIAQSIYDIIGGSSNIVSFTHCMTRLRLVLKDQNNLEMKAIKKIDGVMGVVQQQNQLQIILGPGRVNKVTEEFEKI CGIQTEEVTKEVEFGDGSKLREEIKEKNATPFKLFLKKLSNIFIPLIPAFIACGLVTAILNIVLKLDPAFAETTIGGLLK IAGNAVFWGLNLFVGVNAAKEFGGSPMLGGTMAAIISHPELAKVTLSGQKLLPGRGGIIAVLLVVAFSSFLEKKVRKIMP DALDLFMTPLIVILVSTFVSIFALQPAGGFISDAIGNGTKAAISGGGAVTGFILGGFFLPLVMTGLHQGLTPIHADLLKT TGLNPLLPILAMAGAGQVGAAIAVLLRTKNKRLKKTIISALPVGIMGVGEPLIYGVTLPLGKPFIGACIGGAVGGAVQAL FKVSSTSMGLSGLPLAAITSTPLLFIIGLGAAYIGGFFATLLLGFEDPIE
Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]
COG id: COG1263
COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific
Gene ontology:
Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 PTS EIIC type-1 domain [H]
Homologues:
Organism=Escherichia coli, GI1788769, Length=467, Percent_Identity=33.4047109207709, Blast_Score=216, Evalue=2e-57, Organism=Escherichia coli, GI1790159, Length=470, Percent_Identity=29.1489361702128, Blast_Score=189, Evalue=4e-49, Organism=Escherichia coli, GI2367362, Length=439, Percent_Identity=26.6514806378132, Blast_Score=160, Evalue=2e-40, Organism=Escherichia coli, GI48994906, Length=471, Percent_Identity=29.0870488322718, Blast_Score=158, Evalue=8e-40,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR018113 - InterPro: IPR001996 - InterPro: IPR003352 - InterPro: IPR013013 [H]
Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]
EC number: =2.7.1.69 [H]
Molecular weight: Translated: 47175; Mature: 47044
Theoretical pI: Translated: 9.86; Mature: 9.86
Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 3.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSNVKDIAQSIYDIIGGSSNIVSFTHCMTRLRLVLKDQNNLEMKAIKKIDGVMGVVQQQN CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC QLQIILGPGRVNKVTEEFEKICGIQTEEVTKEVEFGDGSKLREEIKEKNATPFKLFLKKL CEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHH SNIFIPLIPAFIACGLVTAILNIVLKLDPAFAETTIGGLLKIAGNAVFWGLNLFVGVNAA HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHEEEHHHHEECCHH KEFGGSPMLGGTMAAIISHPELAKVTLSGQKLLPGRGGIIAVLLVVAFSSFLEKKVRKIM HHCCCCCCCCHHHHHHHCCCCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHC PDALDLFMTPLIVILVSTFVSIFALQPAGGFISDAIGNGTKAAISGGGAVTGFILGGFFL HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHH PLVMTGLHQGLTPIHADLLKTTGLNPLLPILAMAGAGQVGAAIAVLLRTKNKRLKKTIIS HHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHH ALPVGIMGVGEPLIYGVTLPLGKPFIGACIGGAVGGAVQALFKVSSTSMGLSGLPLAAIT HHCCCHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH STPLLFIIGLGAAYIGGFFATLLLGFEDPIE HCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC >Mature Secondary Structure SNVKDIAQSIYDIIGGSSNIVSFTHCMTRLRLVLKDQNNLEMKAIKKIDGVMGVVQQQN CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC QLQIILGPGRVNKVTEEFEKICGIQTEEVTKEVEFGDGSKLREEIKEKNATPFKLFLKKL CEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHH SNIFIPLIPAFIACGLVTAILNIVLKLDPAFAETTIGGLLKIAGNAVFWGLNLFVGVNAA HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHEEEHHHHEECCHH KEFGGSPMLGGTMAAIISHPELAKVTLSGQKLLPGRGGIIAVLLVVAFSSFLEKKVRKIM HHCCCCCCCCHHHHHHHCCCCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHC PDALDLFMTPLIVILVSTFVSIFALQPAGGFISDAIGNGTKAAISGGGAVTGFILGGFFL HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHH PLVMTGLHQGLTPIHADLLKTTGLNPLLPILAMAGAGQVGAAIAVLLRTKNKRLKKTIIS HHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHH ALPVGIMGVGEPLIYGVTLPLGKPFIGACIGGAVGGAVQALFKVSSTSMGLSGLPLAAIT HHCCCHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH STPLLFIIGLGAAYIGGFFATLLLGFEDPIE HCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 9384377 [H]