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The map label for this gene is hybA [H]

Identifier: 226948612

GI number: 226948612

Start: 1564332

End: 1564775

Strand: Direct

Name: hybA [H]

Synonym: CLM_1507

Alternate gene names: 226948612

Gene position: 1564332-1564775 (Clockwise)

Preceding gene: 226948611

Following gene: 226948613

Centisome position: 37.65

GC content: 31.98

Gene sequence:

>444_bases
ATGAGAAGAATTATGATAAAAAAGGATTTATGTGAAGGATGCCTAAATTGTGTTCTTGCCTGTATGTCAGAGCATAATAA
AAGGGGAAAATCCATATATGATTTAGATTTAGAAGATAAGGCTAATGAAAGCAGAAATCATATAGAGTTAGATATGCTAG
GAAACAAAACCCCTATATTTTGTAGACACTGTGATAATCCAGAATGTGTAAATACTTGCACAACTGGAGCTATGAGTAAA
GATGAAAAGACTGGTGTAGTATCTTATAATGAAGATAAATGTGCATCCTGTTTTATGTGTGTAATGAACTGCCCTTATGG
AGTACTAAAGGCAGATGAAAAGAACAGTAAAGTTATAATAAAATGTGATTTATGTAATGAAAGAGAAATACCTAGATGTA
TAGAAAACTGCCCTACAGGAGCTATCTATGTAGAGGAAATTTAA

Upstream 100 bases:

>100_bases
ATATTATTTTAGATCAATGTAAAAATATTAACTATAGAATTAAATTAAAATTAGTTTTTAGAAGAATCTGGTTTTGAAAT
AAGTTAAGGGGGAATTCTTT

Downstream 100 bases:

>100_bases
ATATATACTAAAAAATTAAGTAAGGTACAATGTATTAGCTGAAGTTTTTATTAAAAATAATTAACATTTTATAATTTAAG
AGTATAGATTGTAAAGTAGG

Product: nitrate reductase, iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MRRIMIKKDLCEGCLNCVLACMSEHNKRGKSIYDLDLEDKANESRNHIELDMLGNKTPIFCRHCDNPECVNTCTTGAMSK
DEKTGVVSYNEDKCASCFMCVMNCPYGVLKADEKNSKVIIKCDLCNEREIPRCIENCPTGAIYVEEI

Sequences:

>Translated_147_residues
MRRIMIKKDLCEGCLNCVLACMSEHNKRGKSIYDLDLEDKANESRNHIELDMLGNKTPIFCRHCDNPECVNTCTTGAMSK
DEKTGVVSYNEDKCASCFMCVMNCPYGVLKADEKNSKVIIKCDLCNEREIPRCIENCPTGAIYVEEI
>Mature_147_residues
MRRIMIKKDLCEGCLNCVLACMSEHNKRGKSIYDLDLEDKANESRNHIELDMLGNKTPIFCRHCDNPECVNTCTTGAMSK
DEKTGVVSYNEDKCASCFMCVMNCPYGVLKADEKNSKVIIKCDLCNEREIPRCIENCPTGAIYVEEI

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=166, Percent_Identity=31.9277108433735, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1788811, Length=151, Percent_Identity=30.4635761589404, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI87082179, Length=145, Percent_Identity=31.7241379310345, Blast_Score=77, Evalue=4e-16,
Organism=Escherichia coli, GI87082180, Length=135, Percent_Identity=34.0740740740741, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI2367345, Length=143, Percent_Identity=33.5664335664336, Blast_Score=71, Evalue=3e-14,
Organism=Escherichia coli, GI1787122, Length=147, Percent_Identity=32.6530612244898, Blast_Score=69, Evalue=9e-14,
Organism=Escherichia coli, GI1789067, Length=156, Percent_Identity=32.0512820512821, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1787872, Length=147, Percent_Identity=32.6530612244898, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787749, Length=157, Percent_Identity=28.0254777070064, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI2367245, Length=145, Percent_Identity=28.9655172413793, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI226510944, Length=152, Percent_Identity=30.2631578947368, Blast_Score=64, Evalue=3e-12,
Organism=Escherichia coli, GI1790326, Length=157, Percent_Identity=28.0254777070064, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1787478, Length=82, Percent_Identity=40.2439024390244, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 16616; Mature: 16616

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
15.6 %Cys+Met (Translated Protein)
10.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
15.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIMIKKDLCEGCLNCVLACMSEHNKRGKSIYDLDLEDKANESRNHIELDMLGNKTPIF
CCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCEE
CRHCDNPECVNTCTTGAMSKDEKTGVVSYNEDKCASCFMCVMNCPYGVLKADEKNSKVII
EEECCCCHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEECCCCCCEEEE
KCDLCNEREIPRCIENCPTGAIYVEEI
EEECCCCCCCHHHHHHCCCCEEEEEEC
>Mature Secondary Structure
MRRIMIKKDLCEGCLNCVLACMSEHNKRGKSIYDLDLEDKANESRNHIELDMLGNKTPIF
CCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCEE
CRHCDNPECVNTCTTGAMSKDEKTGVVSYNEDKCASCFMCVMNCPYGVLKADEKNSKVII
EEECCCCHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEECCCCCCEEEE
KCDLCNEREIPRCIENCPTGAIYVEEI
EEECCCCCCCHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]