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The map label for this gene is potD [C]

Identifier: 226948421

GI number: 226948421

Start: 1363689

End: 1364735

Strand: Direct

Name: potD [C]

Synonym: CLM_1305

Alternate gene names: 226948421

Gene position: 1363689-1364735 (Clockwise)

Preceding gene: 226948420

Following gene: 226948422

Centisome position: 32.82

GC content: 30.75

Gene sequence:

>1047_bases
ATGAAAAAATTAAGATCAATAGTTTCTTTATTTTTAATAGGAATTTTGACATTGGGATTAGCTGCCTGTGGTTCAAAAAA
AGAAGAAACTAAAAATACAGAGGAGAAAAAAGATTTAAAAGGTAGCACTATAAAAATAATTGCTACATCAGAAGACTATA
AGCCAGTATTTGAAAAATTCACAAAAGAAACAGGCATAAAAGCTGAGTTTTTATCTATGTCTTCAGGAGAAGTTATATCA
AGAACTAAGGCTGAAGGTGGAAAGCCTATGGCTGATGTATGGTTTGGTGGTGGATTGGATGCATTTATGGATGCGAAAGA
TTCAGGATTATTAGAAAAATACATACCAGAAGAATCTAAAGATATAAAAGAAGAATACAAAGATAAAGAAGGATATTGGA
TAGGAAAAGGTTTAACAATAGTAGGTTTTTTAGTTAATAAAGATGTACTTAAAGAAAAAAATCTACAGGCACCTGAAAAT
TGGGAAGATTTAGTTAAACCAGAATATAAAAATGAGATACTAATGTCTAATCCAGCAGTATCTGGTACAAATTATGCAGT
AGTAAATGCTTTATTGCAACAAAAAGGAAAAGATGCAGGGTGGAAGTACTTTGAAAATTTAAATAAAAATATATCTTACT
ATTCAAAAAGAGGTAAAGATCCAAAGCTAAAGACTACAGCAGGAGAGGTAGCCATAGGTATAACATATATAGATAATTCT
ATAGTAAAATTAGAAAAAGAAAAAAATATGCAGGTTATATATCCAGAGGATGGAATACCATGGGTTGTAGAGGGAATGGC
TATATTTAAAAATGCTAGCAACTTAGAAGGTGCTAAGATATTTGAAAACTGGGTATTAAAAAGAGAAACACAGGAAGAAC
TAGCTAAAATAGAGGGAAAAGATGGAGCAATGCTTGTTAAGCCCGGGATTAAAGGCATAGATTTAAAAGTACCAAAGAAT
AAATTAATGAAAGAGGATTTATCTTCTTTTGGAAAAGACAGAAAAGAAATACTTGATAAGTGGAAAGCTATGGTAGGTAA
CAAGTAA

Upstream 100 bases:

>100_bases
GAAGATGCTAATCAAATAATAAAAATATATAGATAAAAATGTAAAATACGTTAATGTAACTATTTATTTTTCAAGTATTT
AATTTAAGAGGAGGGGGAAA

Downstream 100 bases:

>100_bases
TATGAACAGGAAAAAATTAAGGGTATCTAATAAATTAGATACCCAATTTTATAATATTTTAGATAAGATTCTAATAGTAT
TTATAATATTATTAGTACTT

Product: ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKKLRSIVSLFLIGILTLGLAACGSKKEETKNTEEKKDLKGSTIKIIATSEDYKPVFEKFTKETGIKAEFLSMSSGEVIS
RTKAEGGKPMADVWFGGGLDAFMDAKDSGLLEKYIPEESKDIKEEYKDKEGYWIGKGLTIVGFLVNKDVLKEKNLQAPEN
WEDLVKPEYKNEILMSNPAVSGTNYAVVNALLQQKGKDAGWKYFENLNKNISYYSKRGKDPKLKTTAGEVAIGITYIDNS
IVKLEKEKNMQVIYPEDGIPWVVEGMAIFKNASNLEGAKIFENWVLKRETQEELAKIEGKDGAMLVKPGIKGIDLKVPKN
KLMKEDLSSFGKDRKEILDKWKAMVGNK

Sequences:

>Translated_348_residues
MKKLRSIVSLFLIGILTLGLAACGSKKEETKNTEEKKDLKGSTIKIIATSEDYKPVFEKFTKETGIKAEFLSMSSGEVIS
RTKAEGGKPMADVWFGGGLDAFMDAKDSGLLEKYIPEESKDIKEEYKDKEGYWIGKGLTIVGFLVNKDVLKEKNLQAPEN
WEDLVKPEYKNEILMSNPAVSGTNYAVVNALLQQKGKDAGWKYFENLNKNISYYSKRGKDPKLKTTAGEVAIGITYIDNS
IVKLEKEKNMQVIYPEDGIPWVVEGMAIFKNASNLEGAKIFENWVLKRETQEELAKIEGKDGAMLVKPGIKGIDLKVPKN
KLMKEDLSSFGKDRKEILDKWKAMVGNK
>Mature_348_residues
MKKLRSIVSLFLIGILTLGLAACGSKKEETKNTEEKKDLKGSTIKIIATSEDYKPVFEKFTKETGIKAEFLSMSSGEVIS
RTKAEGGKPMADVWFGGGLDAFMDAKDSGLLEKYIPEESKDIKEEYKDKEGYWIGKGLTIVGFLVNKDVLKEKNLQAPEN
WEDLVKPEYKNEILMSNPAVSGTNYAVVNALLQQKGKDAGWKYFENLNKNISYYSKRGKDPKLKTTAGEVAIGITYIDNS
IVKLEKEKNMQVIYPEDGIPWVVEGMAIFKNASNLEGAKIFENWVLKRETQEELAKIEGKDGAMLVKPGIKGIDLKVPKN
KLMKEDLSSFGKDRKEILDKWKAMVGNK

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38972; Mature: 38972

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLRSIVSLFLIGILTLGLAACGSKKEETKNTEEKKDLKGSTIKIIATSEDYKPVFEKF
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHCCCCEEEEEEECCCCHHHHHHH
TKETGIKAEFLSMSSGEVISRTKAEGGKPMADVWFGGGLDAFMDAKDSGLLEKYIPEESK
HHHCCCCEEEECCCCCCHHHHHHCCCCCCCHHHEECCCHHHHHCCCCCCHHHHHCCCCHH
DIKEEYKDKEGYWIGKGLTIVGFLVNKDVLKEKNLQAPENWEDLVKPEYKNEILMSNPAV
HHHHHHCCCCCEEEECCCEEEHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEECCCCC
SGTNYAVVNALLQQKGKDAGWKYFENLNKNISYYSKRGKDPKLKTTAGEVAIGITYIDNS
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHCCCCCCCEECCCCEEEEEEEECCC
IVKLEKEKNMQVIYPEDGIPWVVEGMAIFKNASNLEGAKIFENWVLKRETQEELAKIEGK
EEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DGAMLVKPGIKGIDLKVPKNKLMKEDLSSFGKDRKEILDKWKAMVGNK
CCCEEECCCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLRSIVSLFLIGILTLGLAACGSKKEETKNTEEKKDLKGSTIKIIATSEDYKPVFEKF
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHCCCCEEEEEEECCCCHHHHHHH
TKETGIKAEFLSMSSGEVISRTKAEGGKPMADVWFGGGLDAFMDAKDSGLLEKYIPEESK
HHHCCCCEEEECCCCCCHHHHHHCCCCCCCHHHEECCCHHHHHCCCCCCHHHHHCCCCHH
DIKEEYKDKEGYWIGKGLTIVGFLVNKDVLKEKNLQAPENWEDLVKPEYKNEILMSNPAV
HHHHHHCCCCCEEEECCCEEEHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEECCCCC
SGTNYAVVNALLQQKGKDAGWKYFENLNKNISYYSKRGKDPKLKTTAGEVAIGITYIDNS
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHCCCCCCCEECCCCEEEEEEEECCC
IVKLEKEKNMQVIYPEDGIPWVVEGMAIFKNASNLEGAKIFENWVLKRETQEELAKIEGK
EEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DGAMLVKPGIKGIDLKVPKNKLMKEDLSSFGKDRKEILDKWKAMVGNK
CCCEEECCCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]