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The map label for this gene is pncA [H]

Identifier: 226948406

GI number: 226948406

Start: 1350318

End: 1350878

Strand: Direct

Name: pncA [H]

Synonym: CLM_1290

Alternate gene names: 226948406

Gene position: 1350318-1350878 (Clockwise)

Preceding gene: 226948405

Following gene: 226948407

Centisome position: 32.5

GC content: 24.6

Gene sequence:

>561_bases
ATGTTTAAATCAATACTTATAATAGATTGCCAAAATGATTTCATATCAGGTTCACTGGCTTGTGATAATGGAGAGATAGC
TGTTAAAAATATTATAAATTTATTAAATATAAATAAAGATTTAAAAGTATTTTATTCTTTAGATTGGCATAGTGAAAATA
ATAAATCTTTTAGAATAAATGGTGGTATATGGCCAGTACATTGTGTAGCCAATAGATTTGGTGCTAAATTAGAGGAGAGT
TTTTATAAAGAATTACAACATAAAAATCATTCACCTAATAATAAAAAAATATTGTTTTTAAAAGGAAAAAATGATTATAT
AGAAGAATATTCAGCCTATGAAGGAGAAAATATTTTAGGTAATAAGATTAATGAAGTATTAGATAAGGATGTAATTGTAT
GTGGTATAGCTTCTGAGTTTTGTGTTAAAGAAACGGTAGAGGACTTAATTAGAAATGGATTTAATGTGGAATTATATATA
GATGGGGTAGCATATGTAAATAAAAAAGAACATGAAAAAACTTTAAATAATTTAAGAGAGCAGGGGGTTAAATTTATTTA
A

Upstream 100 bases:

>100_bases
AACATATATTCTTAAAAATTCATTAAAGTATAAGAATTCTATAATATTTTAAAGATAATTATATGAAAATTAAAATTTTA
GTTAAAGAAAGGGAATAGTT

Downstream 100 bases:

>100_bases
AGTATAATTTTTAAAATTTAGGCTAAAAATTTGATTGAGGGGATATTATAAAATCATCATTAATATTTATGGAGCTTTTC
CTAATTTAGTTTAAATTAAT

Product: pncA family protein

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MFKSILIIDCQNDFISGSLACDNGEIAVKNIINLLNINKDLKVFYSLDWHSENNKSFRINGGIWPVHCVANRFGAKLEES
FYKELQHKNHSPNNKKILFLKGKNDYIEEYSAYEGENILGNKINEVLDKDVIVCGIASEFCVKETVEDLIRNGFNVELYI
DGVAYVNKKEHEKTLNNLREQGVKFI

Sequences:

>Translated_186_residues
MFKSILIIDCQNDFISGSLACDNGEIAVKNIINLLNINKDLKVFYSLDWHSENNKSFRINGGIWPVHCVANRFGAKLEES
FYKELQHKNHSPNNKKILFLKGKNDYIEEYSAYEGENILGNKINEVLDKDVIVCGIASEFCVKETVEDLIRNGFNVELYI
DGVAYVNKKEHEKTLNNLREQGVKFI
>Mature_186_residues
MFKSILIIDCQNDFISGSLACDNGEIAVKNIINLLNINKDLKVFYSLDWHSENNKSFRINGGIWPVHCVANRFGAKLEES
FYKELQHKNHSPNNKKILFLKGKNDYIEEYSAYEGENILGNKINEVLDKDVIVCGIASEFCVKETVEDLIRNGFNVELYI
DGVAYVNKKEHEKTLNNLREQGVKFI

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=195, Percent_Identity=27.6923076923077, Blast_Score=77, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321401, Length=213, Percent_Identity=26.2910798122066, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 21337; Mature: 21337

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKSILIIDCQNDFISGSLACDNGEIAVKNIINLLNINKDLKVFYSLDWHSENNKSFRIN
CCCCEEEEECCCCCCCCEEEECCCCEEHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEE
GGIWPVHCVANRFGAKLEESFYKELQHKNHSPNNKKILFLKGKNDYIEEYSAYEGENILG
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCHH
NKINEVLDKDVIVCGIASEFCVKETVEDLIRNGFNVELYIDGVAYVNKKEHEKTLNNLRE
HHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCHHHHHHHHHHHH
QGVKFI
CCCCCC
>Mature Secondary Structure
MFKSILIIDCQNDFISGSLACDNGEIAVKNIINLLNINKDLKVFYSLDWHSENNKSFRIN
CCCCEEEEECCCCCCCCEEEECCCCEEHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEE
GGIWPVHCVANRFGAKLEESFYKELQHKNHSPNNKKILFLKGKNDYIEEYSAYEGENILG
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCHH
NKINEVLDKDVIVCGIASEFCVKETVEDLIRNGFNVELYIDGVAYVNKKEHEKTLNNLRE
HHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCHHHHHHHHHHHH
QGVKFI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]