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The map label for this gene is dctA [H]

Identifier: 226947818

GI number: 226947818

Start: 726645

End: 727898

Strand: Direct

Name: dctA [H]

Synonym: CLM_0670

Alternate gene names: 226947818

Gene position: 726645-727898 (Clockwise)

Preceding gene: 226947817

Following gene: 226947819

Centisome position: 17.49

GC content: 31.82

Gene sequence:

>1254_bases
ATGAAAGTATGGGAAAATTATAAATGGTCTATAATACTACTTATCTCTGTAATTATAGGAGGAATAGTTGGATTAATTGC
AGGACCAAGAGCTGAAGTAGTAGAACCTTTAGGTAACTTATTTTTAAATTTAATGTTTACTGCAATTGTGCCCTTAGTAT
TTTTTAGTGTGGCTTCAGCAATTGCTAATATGGCTAATATGAAAAGACTAGGAAAGATTTTATTAAGTGTATTTATAGTA
TTTACTTTTACCGCATTAATTGCAGCTGTAATTGGTGTTATAGGAATCTTAATAATAAACCCTACAAAGGGTGTAGATCC
TTCAACTATGCAAAAGATAATGGCAAATACAGTTAATGAAGTTCAAAATAAAGAGAAGGTAAGTATGTTACAACAAATTG
TAAATACAGTTACAGTATCGGATTTTGCATTATTATTTTCTAGAAAAAATATGCTACAAATTATTATATTCTCAATAATC
TTTGGTGTTTCTACAGCTATGGTTGGAGAAAAGGGTAAACCTGTTGCTAAGTTTTTAGAAGCTGGTTCAACTGTAATGAT
AAAAATGATAGATATAATAATGTATACAGCACCAATAGGACTTGGTTGTTATTTTGCTTCAGTAATAGGACAACTTGGTC
CACAAATAATTGAAGGATATGTGAGAGTGTTTATATTATATCTGATACTTACTTTAGTATATTATTTTATTATGTTTAGC
ATATATGCATATATGTCAGCAGGAAAAACAGGAGTGCAACTATTTTGGAAGAATGCATTGGCTCCTTCAATAACATCTCT
TGCTACCTGTTCTAGTGCAGCATGTATTCCTGTTAATTTAGAAAGCACAAGAAATATGGGTGTACCAAAGGATATTAGAG
AAACAGTTATACCCTTAGGAGCAAACACTCATAAAGATGGCTCTGTTATAGGTGGAGTTTTAAAAATAACTTTTTTACTT
GGATTATTTGGTCATGATATGACTAGTTTCAAAGCAATAATTACAATAATTGTAGTGTCTTATTTAATAGGCGCTGTTAT
GGGAGCTATTCCTGGCGGAGGATTAATAGGTGCTATGCTTATCATAAGCATATATGGTTTTGCCCCTGAAGTTTTAGGAA
TAGTGGCAGTCATAAGCACTATAATAGATACTCCAGCAACTTTGTTAAATTCCACAGGAAATACAGTATGCGCTATGATG
ATAACAAGACTTGTGGAAGGAAAGAATTGGGCTGTTAAGAAAATTACAGCGTAA

Upstream 100 bases:

>100_bases
AGGTAGAATTCTATAGGAAATAAAGAATTTTTGTTATTGTGCTTGGATTATGAAAGTAAAAATAGATTTTATAAAGTTAT
ATATATTGGGAGGAGATAGA

Downstream 100 bases:

>100_bases
AAAGGGTGTAACCATCCTTGGTTTAAAAAGAAATGTTTGGTTACAAAGGCAATTCACTTCAATAAGAGATTCTAATTTGT
TTTTTCTTAAAATATATGAT

Product: proton/sodium-glutamate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MKVWENYKWSIILLISVIIGGIVGLIAGPRAEVVEPLGNLFLNLMFTAIVPLVFFSVASAIANMANMKRLGKILLSVFIV
FTFTALIAAVIGVIGILIINPTKGVDPSTMQKIMANTVNEVQNKEKVSMLQQIVNTVTVSDFALLFSRKNMLQIIIFSII
FGVSTAMVGEKGKPVAKFLEAGSTVMIKMIDIIMYTAPIGLGCYFASVIGQLGPQIIEGYVRVFILYLILTLVYYFIMFS
IYAYMSAGKTGVQLFWKNALAPSITSLATCSSAACIPVNLESTRNMGVPKDIRETVIPLGANTHKDGSVIGGVLKITFLL
GLFGHDMTSFKAIITIIVVSYLIGAVMGAIPGGGLIGAMLIISIYGFAPEVLGIVAVISTIIDTPATLLNSTGNTVCAMM
ITRLVEGKNWAVKKITA

Sequences:

>Translated_417_residues
MKVWENYKWSIILLISVIIGGIVGLIAGPRAEVVEPLGNLFLNLMFTAIVPLVFFSVASAIANMANMKRLGKILLSVFIV
FTFTALIAAVIGVIGILIINPTKGVDPSTMQKIMANTVNEVQNKEKVSMLQQIVNTVTVSDFALLFSRKNMLQIIIFSII
FGVSTAMVGEKGKPVAKFLEAGSTVMIKMIDIIMYTAPIGLGCYFASVIGQLGPQIIEGYVRVFILYLILTLVYYFIMFS
IYAYMSAGKTGVQLFWKNALAPSITSLATCSSAACIPVNLESTRNMGVPKDIRETVIPLGANTHKDGSVIGGVLKITFLL
GLFGHDMTSFKAIITIIVVSYLIGAVMGAIPGGGLIGAMLIISIYGFAPEVLGIVAVISTIIDTPATLLNSTGNTVCAMM
ITRLVEGKNWAVKKITA
>Mature_417_residues
MKVWENYKWSIILLISVIIGGIVGLIAGPRAEVVEPLGNLFLNLMFTAIVPLVFFSVASAIANMANMKRLGKILLSVFIV
FTFTALIAAVIGVIGILIINPTKGVDPSTMQKIMANTVNEVQNKEKVSMLQQIVNTVTVSDFALLFSRKNMLQIIIFSII
FGVSTAMVGEKGKPVAKFLEAGSTVMIKMIDIIMYTAPIGLGCYFASVIGQLGPQIIEGYVRVFILYLILTLVYYFIMFS
IYAYMSAGKTGVQLFWKNALAPSITSLATCSSAACIPVNLESTRNMGVPKDIRETVIPLGANTHKDGSVIGGVLKITFLL
GLFGHDMTSFKAIITIIVVSYLIGAVMGAIPGGGLIGAMLIISIYGFAPEVLGIVAVISTIIDTPATLLNSTGNTVCAMM
ITRLVEGKNWAVKKITA

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI40254478, Length=474, Percent_Identity=21.9409282700422, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI169790839, Length=268, Percent_Identity=23.5074626865672, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI223468564, Length=260, Percent_Identity=22.6923076923077, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI223468566, Length=260, Percent_Identity=22.6923076923077, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI21314632, Length=278, Percent_Identity=23.7410071942446, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI5032093, Length=260, Percent_Identity=22.6923076923077, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI4827012, Length=268, Percent_Identity=22.7611940298507, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI66773030, Length=277, Percent_Identity=25.2707581227437, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI194239697, Length=469, Percent_Identity=20.682302771855, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI262359914, Length=209, Percent_Identity=24.8803827751196, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1789947, Length=408, Percent_Identity=26.2254901960784, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790514, Length=409, Percent_Identity=22.9828850855746, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788024, Length=293, Percent_Identity=25.938566552901, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17537407, Length=416, Percent_Identity=23.0769230769231, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI193206505, Length=436, Percent_Identity=21.3302752293578, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983099, Length=404, Percent_Identity=21.039603960396, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71983106, Length=404, Percent_Identity=21.039603960396, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71996953, Length=418, Percent_Identity=21.0526315789474, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI193206654, Length=412, Percent_Identity=23.0582524271845, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24583025, Length=450, Percent_Identity=19.7777777777778, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17137668, Length=450, Percent_Identity=19.7777777777778, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24583023, Length=450, Percent_Identity=19.7777777777778, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 44711; Mature: 44711

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]