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The map label for this gene is mazF [H]

Identifier: 226947787

GI number: 226947787

Start: 692325

End: 692915

Strand: Direct

Name: mazF [H]

Synonym: CLM_0637

Alternate gene names: 226947787

Gene position: 692325-692915 (Clockwise)

Preceding gene: 226947783

Following gene: 226947788

Centisome position: 16.66

GC content: 29.78

Gene sequence:

>591_bases
ATGAAAAACTTAAATGATATGAACAGTGAAGAATTAGGAAAATACATAAAAGATACAGAAAATCAGATACATAATTTATT
AGATGAATACATAAATAGGGTTAACAATAAAATAGATAAAAATAAGAATGCTAAAACTTTGAAAGAGAAAGCATATGCAT
TAAGTAAACTATATAAATATGTAGAATGGGTAAATGATGGTATAGAAATGAATAATAATGTCAAGAATAGGATTAGGATA
GTTCCTAAACGAGGTGAAGTTTGGACCTGTGAATTAGGTCAAAATATAGGATCGGAAGAAAATAAAATTAGACCGGTTAT
TATTATACAAAATGACACAGGGAACCAAAATGGACCTACAACCATAGTAGTACCCATTTCTAATAGGCCAAAGAAGATAG
CTGTACATATTTCCATAAGAAATGGAGATTTTGAATTAGCTAAGGGAGAAAAAATGGAAATAACAGGAACAGTGTTAGCG
GAACAAATAAGAATTGTATCAAAAGCTAGACTTGGAAGACACGTAGCCACTTTATCAGACAAATTCATGCAGCTTTTGGA
TTCTAAAATAAAAATTTCGCTAGATTTGTAG

Upstream 100 bases:

>100_bases
TATAAATAAAGTAGGTAAATGATAGATAAATAGATTATTAATAAATTTTATTTCTCTTTCAAATTTTGGTATTATATAAT
TGTTATAGGGGGAAAGAGCA

Downstream 100 bases:

>100_bases
TTAAAACAAAAATTATAAAGAATTTAATAAAAAAATATTAAATTTTTATAAATTTATGTTGTATAATTTTTTGTTTAATG
ATATAATAACAATTGTGTTC

Product: PemK family protein

Products: NA

Alternate protein names: Endoribonuclease MazF; Toxin MazF [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MKNLNDMNSEELGKYIKDTENQIHNLLDEYINRVNNKIDKNKNAKTLKEKAYALSKLYKYVEWVNDGIEMNNNVKNRIRI
VPKRGEVWTCELGQNIGSEENKIRPVIIIQNDTGNQNGPTTIVVPISNRPKKIAVHISIRNGDFELAKGEKMEITGTVLA
EQIRIVSKARLGRHVATLSDKFMQLLDSKIKISLDL

Sequences:

>Translated_196_residues
MKNLNDMNSEELGKYIKDTENQIHNLLDEYINRVNNKIDKNKNAKTLKEKAYALSKLYKYVEWVNDGIEMNNNVKNRIRI
VPKRGEVWTCELGQNIGSEENKIRPVIIIQNDTGNQNGPTTIVVPISNRPKKIAVHISIRNGDFELAKGEKMEITGTVLA
EQIRIVSKARLGRHVATLSDKFMQLLDSKIKISLDL
>Mature_196_residues
MKNLNDMNSEELGKYIKDTENQIHNLLDEYINRVNNKIDKNKNAKTLKEKAYALSKLYKYVEWVNDGIEMNNNVKNRIRI
VPKRGEVWTCELGQNIGSEENKIRPVIIIQNDTGNQNGPTTIVVPISNRPKKIAVHISIRNGDFELAKGEKMEITGTVLA
EQIRIVSKARLGRHVATLSDKFMQLLDSKIKISLDL

Specific function: Part of a toxin-antitoxin (TA) module. Ribosome- independent, sequence-specific endoribonuclease that cleaves mRNA, thus inhibiting protein synthesis and inducing bacterial stasis. Cleavages occur preferentially in a U-rich region with a consensus sequenc

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 22384; Mature: 22384

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLNDMNSEELGKYIKDTENQIHNLLDEYINRVNNKIDKNKNAKTLKEKAYALSKLYKY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VEWVNDGIEMNNNVKNRIRIVPKRGEVWTCELGQNIGSEENKIRPVIIIQNDTGNQNGPT
HHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCE
TIVVPISNRPKKIAVHISIRNGDFELAKGEKMEITGTVLAEQIRIVSKARLGRHVATLSD
EEEEECCCCCCEEEEEEEEECCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
KFMQLLDSKIKISLDL
HHHHHHCCCEEEEECC
>Mature Secondary Structure
MKNLNDMNSEELGKYIKDTENQIHNLLDEYINRVNNKIDKNKNAKTLKEKAYALSKLYKY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VEWVNDGIEMNNNVKNRIRIVPKRGEVWTCELGQNIGSEENKIRPVIIIQNDTGNQNGPT
HHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCE
TIVVPISNRPKKIAVHISIRNGDFELAKGEKMEITGTVLAEQIRIVSKARLGRHVATLSD
EEEEECCCCCCEEEEEEEEECCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
KFMQLLDSKIKISLDL
HHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA