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The map label for this gene is entB [C]

Identifier: 226947478

GI number: 226947478

Start: 298381

End: 299055

Strand: Reverse

Name: entB [C]

Synonym: CLM_0310

Alternate gene names: 226947478

Gene position: 299055-298381 (Counterclockwise)

Preceding gene: 226947480

Following gene: 226947457

Centisome position: 7.2

GC content: 37.33

Gene sequence:

>675_bases
GTGATATATTTTTGTCCTATTTGTGGTTACTGGGTTGAATATCCCAGCGATGATAATCTTAACTATAGTGGATACTGGCC
TCCTAGGAACATAAGGGCTGAAGTAACAAATCAAACAATCATTGATAAATGGAATACCGTATCTCCCCCTCCTGCACCTG
AACTGAAATCAGTAGCAGTTGATCCGAAAACCAGTGCCCTATTAATATTAGATATGGAGACCTCTATATGCAAAAGCCCT
CGCTGTATAGGCTCTATCCCTAATATTCATAATCTATTGACGAAAGCCCGTAAAAATAACATGCTAGTTGTGTATAGTCT
TACACATTCGGGAAATCCTAGAGATATTTTCAGGCACTTAGCCCCTTTGCCAGATGATCCTATTGTGAAATCAAACGTAG
ATAAATTCTATAAAACCAATTTAGAAAAAATATTACAAGAAAAAGGTATTAAAACTGTTGTAATTACTGGTTATGCCGCT
AATGGTGCGGTTCTTCATACAGGAACTAGTGCTGCTTTCCGAGGTTACAATGCTATTATCCCTGTTGACTGCATGTCTGC
TAATAACCTTTATGCCGAACAATACACTGCATGGCATATGCTTAATAGTCCTGGAACTAGAAATCGAGCAGTTCTAACAA
AGGTTGATTTAATTAGCTTTTACCACGATAATTAA

Upstream 100 bases:

>100_bases
ATAATAAGTAAAATCATATATATAATCTTACATTTACAGTAACTTTTTATTATATATTACATAGTATGTAATGATATAAA
ATCATATTAGGAGGATTAAT

Downstream 100 bases:

>100_bases
TAATAAAAGTAAAGTTCAGCTTTAATCTAGTAAAATTAAGTATTAAAAATATATTATATAAGTAAAAAACTTTTATGTAC
TAACCATTAACAATTTTAAG

Product: isochorismate family protein

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: Isochorismatase; Nicotinamidase-Like Amidase; Hydrolase; Isochorismate Family Protein; Isochorismatase Hydrolase Protein; Iisochorismatase Family Protein

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MIYFCPICGYWVEYPSDDNLNYSGYWPPRNIRAEVTNQTIIDKWNTVSPPPAPELKSVAVDPKTSALLILDMETSICKSP
RCIGSIPNIHNLLTKARKNNMLVVYSLTHSGNPRDIFRHLAPLPDDPIVKSNVDKFYKTNLEKILQEKGIKTVVITGYAA
NGAVLHTGTSAAFRGYNAIIPVDCMSANNLYAEQYTAWHMLNSPGTRNRAVLTKVDLISFYHDN

Sequences:

>Translated_224_residues
MIYFCPICGYWVEYPSDDNLNYSGYWPPRNIRAEVTNQTIIDKWNTVSPPPAPELKSVAVDPKTSALLILDMETSICKSP
RCIGSIPNIHNLLTKARKNNMLVVYSLTHSGNPRDIFRHLAPLPDDPIVKSNVDKFYKTNLEKILQEKGIKTVVITGYAA
NGAVLHTGTSAAFRGYNAIIPVDCMSANNLYAEQYTAWHMLNSPGTRNRAVLTKVDLISFYHDN
>Mature_224_residues
MIYFCPICGYWVEYPSDDNLNYSGYWPPRNIRAEVTNQTIIDKWNTVSPPPAPELKSVAVDPKTSALLILDMETSICKSP
RCIGSIPNIHNLLTKARKNNMLVVYSLTHSGNPRDIFRHLAPLPDDPIVKSNVDKFYKTNLEKILQEKGIKTVVITGYAA
NGAVLHTGTSAAFRGYNAIIPVDCMSANNLYAEQYTAWHMLNSPGTRNRAVLTKVDLISFYHDN

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 25103; Mature: 25103

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFCPICGYWVEYPSDDNLNYSGYWPPRNIRAEVTNQTIIDKWNTVSPPPAPELKSVAV
CEEECCCCCCEEECCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCCCEEEE
DPKTSALLILDMETSICKSPRCIGSIPNIHNLLTKARKNNMLVVYSLTHSGNPRDIFRHL
CCCCCEEEEEECCHHHHCCCCHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
APLPDDPIVKSNVDKFYKTNLEKILQEKGIKTVVITGYAANGAVLHTGTSAAFRGYNAII
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCHHHHCCCCEEE
PVDCMSANNLYAEQYTAWHMLNSPGTRNRAVLTKVDLISFYHDN
EEEECCCCCEEHHHHEEEEEECCCCCCCCEEEEEEEEEEEEECC
>Mature Secondary Structure
MIYFCPICGYWVEYPSDDNLNYSGYWPPRNIRAEVTNQTIIDKWNTVSPPPAPELKSVAV
CEEECCCCCCEEECCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCCCEEEE
DPKTSALLILDMETSICKSPRCIGSIPNIHNLLTKARKNNMLVVYSLTHSGNPRDIFRHL
CCCCCEEEEEECCHHHHCCCCHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
APLPDDPIVKSNVDKFYKTNLEKILQEKGIKTVVITGYAANGAVLHTGTSAAFRGYNAII
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCHHHHCCCCEEE
PVDCMSANNLYAEQYTAWHMLNSPGTRNRAVLTKVDLISFYHDN
EEEECCCCCEEHHHHEEEEEECCCCCCCCEEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA