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The map label for this gene is ispF [H]

Identifier: 226947291

GI number: 226947291

Start: 92315

End: 92782

Strand: Reverse

Name: ispF [H]

Synonym: CLM_0110

Alternate gene names: 226947291

Gene position: 92782-92315 (Counterclockwise)

Preceding gene: 226947292

Following gene: 226947277

Centisome position: 2.23

GC content: 29.49

Gene sequence:

>468_bases
ATGAGAATAGGATTGGGATACGATGTACATAAACTAGTTGAAGATAGACCACTAATAATAGGTGGTGTTACAATTCCCCA
TGATAAAGGTTTACTAGGTCATTCTGATGCAGATGTATTGGTCCATGCAATTATGGACGCTTTATTAGGAGCCGCGGCAT
TAGGTGATATAGGTAAGCATTTTCCTGATTCAGATAAAAATTTTAAAAATATATCAAGTCTATTACTTTTATCTAAAGTT
AAAGATTTAATAAACAAAGAAGGTTATAAAATTGTAAATATAGACTGTACTATAATAGCTCAAAAGCCTAAAATGCTTTA
TCATATAGATGCTATGAAAAAAAATATTTGTAAGTGTTTAAAATTAGATAATAATATGTTAAATATAAAAGCTACTACAG
AAGAGGGTTTAGGTTTTACAGGTAAAGAAGAAGGAATCTCCGCTAATGCTATTTGTCTTTTGGATTAA

Upstream 100 bases:

>100_bases
AAATGATAATAAAAATATATCTTAAGGTGCAACTTTGTTGCACTTTTTTTTAAAGTATATATCATTATATATATAATATT
AAATTATTGGAGGTTTTTTA

Downstream 100 bases:

>100_bases
GACTGTAAATTAAAAAATATTTAATTTACCAAAAATATAAGAGTTCACCTTAATTTATAAGTGAACTCTTATTTGAATTT
ATATTTTATTTAAGATCTTT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MRIGLGYDVHKLVEDRPLIIGGVTIPHDKGLLGHSDADVLVHAIMDALLGAAALGDIGKHFPDSDKNFKNISSLLLLSKV
KDLINKEGYKIVNIDCTIIAQKPKMLYHIDAMKKNICKCLKLDNNMLNIKATTEEGLGFTGKEEGISANAICLLD

Sequences:

>Translated_155_residues
MRIGLGYDVHKLVEDRPLIIGGVTIPHDKGLLGHSDADVLVHAIMDALLGAAALGDIGKHFPDSDKNFKNISSLLLLSKV
KDLINKEGYKIVNIDCTIIAQKPKMLYHIDAMKKNICKCLKLDNNMLNIKATTEEGLGFTGKEEGISANAICLLD
>Mature_155_residues
MRIGLGYDVHKLVEDRPLIIGGVTIPHDKGLLGHSDADVLVHAIMDALLGAAALGDIGKHFPDSDKNFKNISSLLLLSKV
KDLINKEGYKIVNIDCTIIAQKPKMLYHIDAMKKNICKCLKLDNNMLNIKATTEEGLGFTGKEEGISANAICLLD

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=55.8441558441558, Blast_Score=152, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 16864; Mature: 16864

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGLGYDVHKLVEDRPLIIGGVTIPHDKGLLGHSDADVLVHAIMDALLGAAALGDIGKH
CCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FPDSDKNFKNISSLLLLSKVKDLINKEGYKIVNIDCTIIAQKPKMLYHIDAMKKNICKCL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCEEEEHHHHHHHHHHHH
KLDNNMLNIKATTEEGLGFTGKEEGISANAICLLD
HCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MRIGLGYDVHKLVEDRPLIIGGVTIPHDKGLLGHSDADVLVHAIMDALLGAAALGDIGKH
CCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FPDSDKNFKNISSLLLLSKVKDLINKEGYKIVNIDCTIIAQKPKMLYHIDAMKKNICKCL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCEEEEHHHHHHHHHHHH
KLDNNMLNIKATTEEGLGFTGKEEGISANAICLLD
HCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA