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The map label for this gene is yaaO [H]

Identifier: 226947279

GI number: 226947279

Start: 80956

End: 82404

Strand: Direct

Name: yaaO [H]

Synonym: CLM_0096

Alternate gene names: 226947279

Gene position: 80956-82404 (Clockwise)

Preceding gene: 226947278

Following gene: 226947280

Centisome position: 1.95

GC content: 25.6

Gene sequence:

>1449_bases
ATGGGAGAATTACCGATTTTAAATGGAGTTTTAAAATACATTAAGGAAAAAAACATATTGTTTTCTATGCCAGGACATAA
AGGATCCTTAGGATTTTTATTTACTAAAGAGGGGAAAAAGTTTTGTGAAAATATGCCTCAATGTGATTTAACAGAAGTTG
AAGAACTAGACAATCTTCATAATAGTGAAGGAATAATAAAAGAGTCCGGCTATCTTTTAAGCCGGTTTTATAATTCTAAA
AAATCTTATTTTTTAGTAAATGGTAGTACTAGTGGAAACTTAGCTATGATATTTTCTTGTTTTAAAGAAGAGGATAAAAT
CATTGTAGAAAGAAATTGCCATAGATCTATATTTAATGGAATAATTATGAGAAAATTAAAACCAATATATATAAAAAATA
AGGTGTATAGGCAATTTAATGCTCCACTTTCAATAGATTTGGAGCATTTTTTAAGTTTAATAAAAGAAAATAAAGATGCA
AAGGGGATAGTAATAACTTATCCTAATTATTATGGAGTTTGTCCTAATTTAAAAGTTATAATAAAAGAGGCTAAAAAATA
TGACATGAAAGTATTAATAGATTCAGCTCATGGAGCTCATTTTGGTGTGTGTGAAAAGTTACCTAAAAGTGCCCTAGAGT
TAGGAGCAGACATGGTAGTAATGAGTGCCCATAAAACCCTTCCTAGTTTAACACAAACAGCATTTTTACATATAGGCAAA
GATTCTATAATTGATATTAATAAGGTAGATTTTTACATAAGTGCTTTTTCTAGTACTAGTCCTTCTTACTTATTTTTATG
TTCTATGGATTATAGTAGGTTTTATTTAGAAAAATATGGACAAGAACATTATAGTGAATTGATTAATAGAGCAAATTATT
ATAGAAAAAAAATAAATTTATTAGATAGTTTTTATGTATTAAATCATAGTGATATAAAACATTTAAATCTTAAATATAAA
GACGTTATGGATATAGATTTGACAAGATATACAATCAATATAAAAAACGGTTTAAATGCTAGTTGTTTATCTAAGTATTT
AAGAGAATGTGGAATACAATGTGAAATGAGCGATGGAAACAACATAATTTTAATATTGTCTCCTTTTTATAATGAAGATG
TCATGGATAGATTGTATGAGTCTTTATATGAGTGGGACAAAAATTATAATAAAGACAAATATAATTATAATATAAATTAT
AATTATATAGAAACTAATATGAATATGTATCCTTATGAAGTTTTAGAAAAAGAATATTTATGGATAGACTATAAGGATTC
ATTAGGCAAAATAAGTTATAATAATATAGTTCCCTACCCTCCGGGCGTTCCAATAATAATGGCAGGAGAGATAATAAATA
AAGAAATAATAGATGCTATATGTTATTATAAAAATAATGGAATAGATGTATTAGGTTTAAAGGATAATAAGATTCAAATA
GTAAAATAA

Upstream 100 bases:

>100_bases
GAATGGTAAATAGTGATATGAGTACAGATTTTTATGAGTTTTATAAAAAATGTATAAGAAAAAATTTAGTGCAAACTTTT
ATAAAGGATGAGGAAATGAG

Downstream 100 bases:

>100_bases
TAAGTATAAATGTGGAGGAAAAAATGGAAAGAGGTAGGTTAATAGTTATAGAGGGTAGTGATGGAAGTGGTAAAGCTACC
CAAACTAAAAAACTTTATGA

Product: Orn/Lys/Arg decarboxylase

Products: 1,5-Diaminopentane; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MGELPILNGVLKYIKEKNILFSMPGHKGSLGFLFTKEGKKFCENMPQCDLTEVEELDNLHNSEGIIKESGYLLSRFYNSK
KSYFLVNGSTSGNLAMIFSCFKEEDKIIVERNCHRSIFNGIIMRKLKPIYIKNKVYRQFNAPLSIDLEHFLSLIKENKDA
KGIVITYPNYYGVCPNLKVIIKEAKKYDMKVLIDSAHGAHFGVCEKLPKSALELGADMVVMSAHKTLPSLTQTAFLHIGK
DSIIDINKVDFYISAFSSTSPSYLFLCSMDYSRFYLEKYGQEHYSELINRANYYRKKINLLDSFYVLNHSDIKHLNLKYK
DVMDIDLTRYTINIKNGLNASCLSKYLRECGIQCEMSDGNNIILILSPFYNEDVMDRLYESLYEWDKNYNKDKYNYNINY
NYIETNMNMYPYEVLEKEYLWIDYKDSLGKISYNNIVPYPPGVPIIMAGEIINKEIIDAICYYKNNGIDVLGLKDNKIQI
VK

Sequences:

>Translated_482_residues
MGELPILNGVLKYIKEKNILFSMPGHKGSLGFLFTKEGKKFCENMPQCDLTEVEELDNLHNSEGIIKESGYLLSRFYNSK
KSYFLVNGSTSGNLAMIFSCFKEEDKIIVERNCHRSIFNGIIMRKLKPIYIKNKVYRQFNAPLSIDLEHFLSLIKENKDA
KGIVITYPNYYGVCPNLKVIIKEAKKYDMKVLIDSAHGAHFGVCEKLPKSALELGADMVVMSAHKTLPSLTQTAFLHIGK
DSIIDINKVDFYISAFSSTSPSYLFLCSMDYSRFYLEKYGQEHYSELINRANYYRKKINLLDSFYVLNHSDIKHLNLKYK
DVMDIDLTRYTINIKNGLNASCLSKYLRECGIQCEMSDGNNIILILSPFYNEDVMDRLYESLYEWDKNYNKDKYNYNINY
NYIETNMNMYPYEVLEKEYLWIDYKDSLGKISYNNIVPYPPGVPIIMAGEIINKEIIDAICYYKNNGIDVLGLKDNKIQI
VK
>Mature_481_residues
GELPILNGVLKYIKEKNILFSMPGHKGSLGFLFTKEGKKFCENMPQCDLTEVEELDNLHNSEGIIKESGYLLSRFYNSKK
SYFLVNGSTSGNLAMIFSCFKEEDKIIVERNCHRSIFNGIIMRKLKPIYIKNKVYRQFNAPLSIDLEHFLSLIKENKDAK
GIVITYPNYYGVCPNLKVIIKEAKKYDMKVLIDSAHGAHFGVCEKLPKSALELGADMVVMSAHKTLPSLTQTAFLHIGKD
SIIDINKVDFYISAFSSTSPSYLFLCSMDYSRFYLEKYGQEHYSELINRANYYRKKINLLDSFYVLNHSDIKHLNLKYKD
VMDIDLTRYTINIKNGLNASCLSKYLRECGIQCEMSDGNNIILILSPFYNEDVMDRLYESLYEWDKNYNKDKYNYNINYN
YIETNMNMYPYEVLEKEYLWIDYKDSLGKISYNNIVPYPPGVPIIMAGEIINKEIIDAICYYKNNGIDVLGLKDNKIQIV
K

Specific function: Appears To Play A Role In pH Homeostasis By Consuming Protons And Neutralizing The Acidic By-Products Of Carbohydrate Fermentation. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI1790573, Length=550, Percent_Identity=24.1818181818182, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786384, Length=546, Percent_Identity=21.978021978022, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI221142684, Length=327, Percent_Identity=24.7706422018349, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C [H]

EC number: 4.1.1.18 [C]

Molecular weight: Translated: 55861; Mature: 55730

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGELPILNGVLKYIKEKNILFSMPGHKGSLGFLFTKEGKKFCENMPQCDLTEVEELDNLH
CCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCHHHHHCCCCCCCHHHHHHHHCC
NSEGIIKESGYLLSRFYNSKKSYFLVNGSTSGNLAMIFSCFKEEDKIIVERNCHRSIFNG
CCCCEEECCCHHHHHHHCCCCCEEEEECCCCCCCEEHEEHHCCCCCEEEECCHHHHHHHH
IIMRKLKPIYIKNKVYRQFNAPLSIDLEHFLSLIKENKDAKGIVITYPNYYGVCPNLKVI
HHHHHCCCEEEEHHHHHHHCCCEEECHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHH
IKEAKKYDMKVLIDSAHGAHFGVCEKLPKSALELGADMVVMSAHKTLPSLTQTAFLHIGK
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHCCCHHHHHHEEEECC
DSIIDINKVDFYISAFSSTSPSYLFLCSMDYSRFYLEKYGQEHYSELINRANYYRKKINL
CCEEEEEEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
LDSFYVLNHSDIKHLNLKYKDVMDIDLTRYTINIKNGLNASCLSKYLRECGIQCEMSDGN
HHHEEEECCCCCEEECEEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCC
NIILILSPFYNEDVMDRLYESLYEWDKNYNKDKYNYNINYNYIETNMNMYPYEVLEKEYL
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCHHHHCCEEE
WIDYKDSLGKISYNNIVPYPPGVPIIMAGEIINKEIIDAICYYKNNGIDVLGLKDNKIQI
EEEECCCCCEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHEEECCCEEEEEECCCCEEE
VK
EC
>Mature Secondary Structure 
GELPILNGVLKYIKEKNILFSMPGHKGSLGFLFTKEGKKFCENMPQCDLTEVEELDNLH
CCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCHHHHHCCCCCCCHHHHHHHHCC
NSEGIIKESGYLLSRFYNSKKSYFLVNGSTSGNLAMIFSCFKEEDKIIVERNCHRSIFNG
CCCCEEECCCHHHHHHHCCCCCEEEEECCCCCCCEEHEEHHCCCCCEEEECCHHHHHHHH
IIMRKLKPIYIKNKVYRQFNAPLSIDLEHFLSLIKENKDAKGIVITYPNYYGVCPNLKVI
HHHHHCCCEEEEHHHHHHHCCCEEECHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHH
IKEAKKYDMKVLIDSAHGAHFGVCEKLPKSALELGADMVVMSAHKTLPSLTQTAFLHIGK
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHCCCHHHHHHEEEECC
DSIIDINKVDFYISAFSSTSPSYLFLCSMDYSRFYLEKYGQEHYSELINRANYYRKKINL
CCEEEEEEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
LDSFYVLNHSDIKHLNLKYKDVMDIDLTRYTINIKNGLNASCLSKYLRECGIQCEMSDGN
HHHEEEECCCCCEEECEEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCC
NIILILSPFYNEDVMDRLYESLYEWDKNYNKDKYNYNINYNYIETNMNMYPYEVLEKEYL
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCHHHHCCEEE
WIDYKDSLGKISYNNIVPYPPGVPIIMAGEIINKEIIDAICYYKNNGIDVLGLKDNKIQI
EEEECCCCCEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHEEECCCEEEEEECCCCEEE
VK
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate; Sulfhydryl groups [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.4 {S-aminoethyl-L-Cys}} 2 {L-Lys}} 1.5 {L-Lys}} 7 {delta-hydroxylysine}} [C]

Substrates: H+; L-Lysine [C]

Specific reaction: H+ + L-Lysine --> 1,5-Diaminopentane + CO2 [C]

General reaction: Decarboxylation [C]

Inhibitor: NaBH4; NaCl [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]