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The map label for this gene is gutB [H]

Identifier: 226225214

GI number: 226225214

Start: 2707862

End: 2708893

Strand: Reverse

Name: gutB [H]

Synonym: Lm4b_02636

Alternate gene names: 226225214

Gene position: 2708893-2707862 (Counterclockwise)

Preceding gene: 226225215

Following gene: 226225213

Centisome position: 93.0

GC content: 41.57

Gene sequence:

>1032_bases
TTGAAAGCAGTAGTAAAAACAAATCCCGGATATGATCAAATGGAGCTAAGAGATGTAGAAGAACCACAGGTCTATGGCGA
CAAAGTAAAAATCAAAGTAGCATTCACTGGTATTTGCGGATCAGACATCCATACGTTTAAAGGTGAATACAAAAATCCAA
CAACTCCCGTCACACTGGGACATGAATTTTCTGGTGTCGTTGTAGAAGTTGGGCCAGATGTAACAAGTATCAAAGTGGGA
GACCGTGTCACAAGTGAAACAACTTTTGAAACTTGTGGAGAATGTATTTATTGTAAGGAACGTGATTACAATTTATGTAG
CAATCGTCGCGGCATTGGTACGCAAGCAAATGGTAGTTTTGCAGAATTTGTTTTATCTCGCGAAGAAAGTTGTCACGTGC
TTGATGAACGTATTTCGCTCGAAGCAGCAGCACTAACAGAACCGCTTGCATGTTGTGTGCATTCGGCGCTTGAAAAAACA
ACGATTCGTCCAGATGACACAGTACTTGTTTTCGGACCAGGTCCAATTGGTTTGTTACTAGCTCAAGTTGTGAAAGCGCA
AGGGGCAACGGTGATTATGGCAGGAATTACCAAAGATAGTGACCGCTTACGTCTAGCAAAAGAGCTTGGAATGGACCGGA
TTGTCGATACTTTAAAAGAAGACTTGGCTGAAGTTGTGCTTGGCATGACAGATGGTTACGGAGCGGAACGCGTATTTGAT
TGTTCTGGCGCAGTACCCGCTGTAAATCAAGGATTACCTCTAACGAAGAAAAAAGGCGATTTTGTTCAAGTAGGACTTTT
TGCCGAAAAGAAAAATGCGATTGATGAAGAATCCATTATCCAACGTGAAATTGCTTATATCGGCAGCCGTTCGCAAAAAC
CATCTTCTTGGATTTTAGCGCTCGACTTACTGGCAAATGGCAAAATCAATACGGATAAAATGATTACTAAAGTATACGGT
TTGGATGACTGGCGCGAGGCTTTCGAGGCAGTTATGGCAGGAAATGAAATTAAAGTATTAGTGAAATCTTAA

Upstream 100 bases:

>100_bases
TATAAGCTAGAAGAAACGAAAGAAGCTTTTGATATGATTCTTTCCAGAGAGCATGATTATAACAAAGTGATGATATTGCC
GCAGAAAGGTGACGATTAAT

Downstream 100 bases:

>100_bases
TAATGGAGGATGGGCTGTATGAAAAAACATCAAAAAAAGGATAACCGTGCAAACGAATCCAAAGCTTCTCGGGCTCAGAT
GTTTTACAGTAGTCATCCTT

Product: polyol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKAVVKTNPGYDQMELRDVEEPQVYGDKVKIKVAFTGICGSDIHTFKGEYKNPTTPVTLGHEFSGVVVEVGPDVTSIKVG
DRVTSETTFETCGECIYCKERDYNLCSNRRGIGTQANGSFAEFVLSREESCHVLDERISLEAAALTEPLACCVHSALEKT
TIRPDDTVLVFGPGPIGLLLAQVVKAQGATVIMAGITKDSDRLRLAKELGMDRIVDTLKEDLAEVVLGMTDGYGAERVFD
CSGAVPAVNQGLPLTKKKGDFVQVGLFAEKKNAIDEESIIQREIAYIGSRSQKPSSWILALDLLANGKINTDKMITKVYG
LDDWREAFEAVMAGNEIKVLVKS

Sequences:

>Translated_343_residues
MKAVVKTNPGYDQMELRDVEEPQVYGDKVKIKVAFTGICGSDIHTFKGEYKNPTTPVTLGHEFSGVVVEVGPDVTSIKVG
DRVTSETTFETCGECIYCKERDYNLCSNRRGIGTQANGSFAEFVLSREESCHVLDERISLEAAALTEPLACCVHSALEKT
TIRPDDTVLVFGPGPIGLLLAQVVKAQGATVIMAGITKDSDRLRLAKELGMDRIVDTLKEDLAEVVLGMTDGYGAERVFD
CSGAVPAVNQGLPLTKKKGDFVQVGLFAEKKNAIDEESIIQREIAYIGSRSQKPSSWILALDLLANGKINTDKMITKVYG
LDDWREAFEAVMAGNEIKVLVKS
>Mature_343_residues
MKAVVKTNPGYDQMELRDVEEPQVYGDKVKIKVAFTGICGSDIHTFKGEYKNPTTPVTLGHEFSGVVVEVGPDVTSIKVG
DRVTSETTFETCGECIYCKERDYNLCSNRRGIGTQANGSFAEFVLSREESCHVLDERISLEAAALTEPLACCVHSALEKT
TIRPDDTVLVFGPGPIGLLLAQVVKAQGATVIMAGITKDSDRLRLAKELGMDRIVDTLKEDLAEVVLGMTDGYGAERVFD
CSGAVPAVNQGLPLTKKKGDFVQVGLFAEKKNAIDEESIIQREIAYIGSRSQKPSSWILALDLLANGKINTDKMITKVYG
LDDWREAFEAVMAGNEIKVLVKS

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=346, Percent_Identity=30.3468208092486, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI4501933, Length=360, Percent_Identity=28.6111111111111, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI71565154, Length=378, Percent_Identity=26.1904761904762, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI156523966, Length=377, Percent_Identity=26.525198938992, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI34577061, Length=362, Percent_Identity=28.7292817679558, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI4501929, Length=360, Percent_Identity=28.0555555555556, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI4501939, Length=370, Percent_Identity=26.7567567567568, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI71565152, Length=364, Percent_Identity=25.5494505494505, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI71743840, Length=369, Percent_Identity=27.3712737127371, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI262073058, Length=369, Percent_Identity=27.3712737127371, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1788075, Length=354, Percent_Identity=28.8135593220339, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790045, Length=348, Percent_Identity=31.8965517241379, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1788073, Length=334, Percent_Identity=32.3353293413174, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI226510992, Length=299, Percent_Identity=33.7792642140468, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787863, Length=333, Percent_Identity=27.9279279279279, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790718, Length=312, Percent_Identity=29.4871794871795, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87082125, Length=355, Percent_Identity=27.887323943662, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788407, Length=336, Percent_Identity=25.5952380952381, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081918, Length=310, Percent_Identity=29.6774193548387, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786552, Length=379, Percent_Identity=27.4406332453826, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786825, Length=270, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786518, Length=333, Percent_Identity=25.2252252252252, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17562876, Length=344, Percent_Identity=32.8488372093023, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17562878, Length=325, Percent_Identity=32.9230769230769, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17562584, Length=311, Percent_Identity=27.9742765273312, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71988145, Length=325, Percent_Identity=26.7692307692308, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17562582, Length=283, Percent_Identity=28.9752650176678, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71997431, Length=369, Percent_Identity=26.2872628726287, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25146526, Length=370, Percent_Identity=26.2162162162162, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17565904, Length=375, Percent_Identity=25.0666666666667, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319955, Length=338, Percent_Identity=34.0236686390533, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322619, Length=338, Percent_Identity=34.0236686390533, Blast_Score=146, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323099, Length=355, Percent_Identity=30.9859154929577, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319257, Length=340, Percent_Identity=29.4117647058824, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319258, Length=360, Percent_Identity=27.7777777777778, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320033, Length=378, Percent_Identity=29.1005291005291, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323961, Length=328, Percent_Identity=28.0487804878049, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324486, Length=331, Percent_Identity=27.4924471299094, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319621, Length=328, Percent_Identity=27.4390243902439, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323729, Length=332, Percent_Identity=25.6024096385542, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319520, Length=332, Percent_Identity=28.3132530120482, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323980, Length=207, Percent_Identity=32.3671497584541, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319949, Length=246, Percent_Identity=30.0813008130081, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17137530, Length=348, Percent_Identity=31.3218390804598, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI17737897, Length=349, Percent_Identity=31.8051575931232, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17737895, Length=372, Percent_Identity=27.9569892473118, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI221457811, Length=343, Percent_Identity=27.6967930029155, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI45550770, Length=343, Percent_Identity=27.6967930029155, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI45551930, Length=343, Percent_Identity=27.6967930029155, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 37347; Mature: 37347

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVVKTNPGYDQMELRDVEEPQVYGDKVKIKVAFTGICGSDIHTFKGEYKNPTTPVTLG
CCCEEECCCCCCCCEECCCCCCCCCCCEEEEEEEEEECCCCCCHHCCCCCCCCCCCEEEC
HEFSGVVVEVGPDVTSIKVGDRVTSETTFETCGECIYCKERDYNLCSNRRGIGTQANGSF
CCCCCEEEEECCCEEEEEECCCCCCCCCHHHCCCEEEEECCCCCHHCCCCCCCCCCCCHH
AEFVLSREESCHVLDERISLEAAALTEPLACCVHSALEKTTIRPDDTVLVFGPGPIGLLL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHH
AQVVKAQGATVIMAGITKDSDRLRLAKELGMDRIVDTLKEDLAEVVLGMTDGYGAERVFD
HHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
CSGAVPAVNQGLPLTKKKGDFVQVGLFAEKKNAIDEESIIQREIAYIGSRSQKPSSWILA
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEE
LDLLANGKINTDKMITKVYGLDDWREAFEAVMAGNEIKVLVKS
EEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEC
>Mature Secondary Structure
MKAVVKTNPGYDQMELRDVEEPQVYGDKVKIKVAFTGICGSDIHTFKGEYKNPTTPVTLG
CCCEEECCCCCCCCEECCCCCCCCCCCEEEEEEEEEECCCCCCHHCCCCCCCCCCCEEEC
HEFSGVVVEVGPDVTSIKVGDRVTSETTFETCGECIYCKERDYNLCSNRRGIGTQANGSF
CCCCCEEEEECCCEEEEEECCCCCCCCCHHHCCCEEEEECCCCCHHCCCCCCCCCCCCHH
AEFVLSREESCHVLDERISLEAAALTEPLACCVHSALEKTTIRPDDTVLVFGPGPIGLLL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHH
AQVVKAQGATVIMAGITKDSDRLRLAKELGMDRIVDTLKEDLAEVVLGMTDGYGAERVFD
HHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
CSGAVPAVNQGLPLTKKKGDFVQVGLFAEKKNAIDEESIIQREIAYIGSRSQKPSSWILA
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEE
LDLLANGKINTDKMITKVYGLDDWREAFEAVMAGNEIKVLVKS
EEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]