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The map label for this gene is rpoA

Identifier: 226225152

GI number: 226225152

Start: 2658604

End: 2659548

Strand: Reverse

Name: rpoA

Synonym: Lm4b_02573

Alternate gene names: 226225152

Gene position: 2659548-2658604 (Counterclockwise)

Preceding gene: 226225153

Following gene: 226225151

Centisome position: 91.31

GC content: 37.25

Gene sequence:

>945_bases
ATGATCGAAATTGAAAAGCCAAAAATCGAGACGATTGAGATCAGCGATGATGCCAAGTATGGAAAGTTTGTTGTAGAGCC
ACTTGAGCGTGGATATGGTACAACTTTGGGTAACTCCTTACGTCGTATTCTATTATCTTCTCTTCCAGGTGCAGCAGTAA
CCTCTATCCAAATTGATGGAGCTTTACATGAGTTTTCTGTAATTGAAGGTGTAGTAGAAGATGTAACAACCATGATTTTA
AATATTAAAAAACTTGCACTAAAAATCTATTCTGATGAAGAAAAAACATTAGAAATCGATATGCAAGGTCCTGGTGTAGT
AACTGCAGCTGACATTAATTATGACAGCGACGTTGAGATTTTAAATCCCGACTTACACATTGCTACATTAAGTGATAATG
CTAAATTTCATGTGCGTTTAAATGCGACTCGTGGTCGTGGTTACACACCTGCTGATCAAAATAAACGCGAAAATATGCCA
ATTGGTGTACTTCCAGTCGATTCAATCTTTTCACCGGTTATCCGTGTGAACTATCAAGTGGAAAATACACGTGTTGGACA
ATCAACTAATTATGATAAGCTTACGTTTGATGTGTTAACTGACGGAAGTATCAGCCCAGAAGAAGCAGTTTCACTTGGAG
CTAAAATTCTTTCTGAGCACTTAAGTATCTTCGTTAACTTAACAGATGAAGCACAAAAAGCTGAAATTATGATTGAAAAA
GAAGAAAGCCATAAAGAGAAAGTGCTTGAAATGACTATTGAAGAATTAGACTTGTCTGTTCGTTCATATAATTGTTTGAA
ACGCGCTGGAATCAATACAGTACAGGAACTTGCTGACAAATCCGAAGACGATATGATGAAAGTCCGTAACTTGGGCCGTA
AATCGCTTGAGGAAGTTAAAGTAAAACTGGCTGACCTTGGCTTATCTCTAAGAAACGAAAACTGA

Upstream 100 bases:

>100_bases
TTCTTTTGCCAATAGTAGATTTTTACTGTCTTAACTATCAAGGCAGAGAGTTTGACATAAAGACTGATATTCTAGACGTT
TTGAAGGAGGGTAAATTTGA

Downstream 100 bases:

>100_bases
TAAAGGAGGCAATTCCATGGGTTACAGAAAATTAGGTCGTACAAGCTCACAACGTAAAGCGTTACTACGTGATCTTGCAA
CGGATTTAATCGTATTTGAA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MIEIEKPKIETIEISDDAKYGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVTSIQIDGALHEFSVIEGVVEDVTTMIL
NIKKLALKIYSDEEKTLEIDMQGPGVVTAADINYDSDVEILNPDLHIATLSDNAKFHVRLNATRGRGYTPADQNKRENMP
IGVLPVDSIFSPVIRVNYQVENTRVGQSTNYDKLTFDVLTDGSISPEEAVSLGAKILSEHLSIFVNLTDEAQKAEIMIEK
EESHKEKVLEMTIEELDLSVRSYNCLKRAGINTVQELADKSEDDMMKVRNLGRKSLEEVKVKLADLGLSLRNEN

Sequences:

>Translated_314_residues
MIEIEKPKIETIEISDDAKYGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVTSIQIDGALHEFSVIEGVVEDVTTMIL
NIKKLALKIYSDEEKTLEIDMQGPGVVTAADINYDSDVEILNPDLHIATLSDNAKFHVRLNATRGRGYTPADQNKRENMP
IGVLPVDSIFSPVIRVNYQVENTRVGQSTNYDKLTFDVLTDGSISPEEAVSLGAKILSEHLSIFVNLTDEAQKAEIMIEK
EESHKEKVLEMTIEELDLSVRSYNCLKRAGINTVQELADKSEDDMMKVRNLGRKSLEEVKVKLADLGLSLRNEN
>Mature_314_residues
MIEIEKPKIETIEISDDAKYGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVTSIQIDGALHEFSVIEGVVEDVTTMIL
NIKKLALKIYSDEEKTLEIDMQGPGVVTAADINYDSDVEILNPDLHIATLSDNAKFHVRLNATRGRGYTPADQNKRENMP
IGVLPVDSIFSPVIRVNYQVENTRVGQSTNYDKLTFDVLTDGSISPEEAVSLGAKILSEHLSIFVNLTDEAQKAEIMIEK
EESHKEKVLEMTIEELDLSVRSYNCLKRAGINTVQELADKSEDDMMKVRNLGRKSLEEVKVKLADLGLSLRNEN

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=318, Percent_Identity=45.5974842767296, Blast_Score=259, Evalue=1e-70,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_LISIN (P66700)

Other databases:

- EMBL:   AL596173
- PIR:   AE1776
- RefSeq:   NP_472084.1
- ProteinModelPortal:   P66700
- GeneID:   1131612
- GenomeReviews:   AL592022_GR
- KEGG:   lin:lin2755
- GenoList:   LIN2755
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   LINN272626:LIN2755-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 34906; Mature: 34906

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIEKPKIETIEISDDAKYGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVTSIQIDG
CCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEECC
ALHEFSVIEGVVEDVTTMILNIKKLALKIYSDEEKTLEIDMQGPGVVTAADINYDSDVEI
CHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCEE
LNPDLHIATLSDNAKFHVRLNATRGRGYTPADQNKRENMPIGVLPVDSIFSPVIRVNYQV
ECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCEEEEEEE
ENTRVGQSTNYDKLTFDVLTDGSISPEEAVSLGAKILSEHLSIFVNLTDEAQKAEIMIEK
ECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEEC
EESHKEKVLEMTIEELDLSVRSYNCLKRAGINTVQELADKSEDDMMKVRNLGRKSLEEVK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
VKLADLGLSLRNEN
HHHHHCCEEECCCC
>Mature Secondary Structure
MIEIEKPKIETIEISDDAKYGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVTSIQIDG
CCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEECC
ALHEFSVIEGVVEDVTTMILNIKKLALKIYSDEEKTLEIDMQGPGVVTAADINYDSDVEI
CHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCEE
LNPDLHIATLSDNAKFHVRLNATRGRGYTPADQNKRENMPIGVLPVDSIFSPVIRVNYQV
ECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCEEEEEEE
ENTRVGQSTNYDKLTFDVLTDGSISPEEAVSLGAKILSEHLSIFVNLTDEAQKAEIMIEK
ECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEEC
EESHKEKVLEMTIEELDLSVRSYNCLKRAGINTVQELADKSEDDMMKVRNLGRKSLEEVK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
VKLADLGLSLRNEN
HHHHHCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669