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The map label for this gene is dppE [H]

Identifier: 226225116

GI number: 226225116

Start: 2618477

End: 2620138

Strand: Reverse

Name: dppE [H]

Synonym: Lm4b_02537

Alternate gene names: 226225116

Gene position: 2620138-2618477 (Counterclockwise)

Preceding gene: 226225117

Following gene: 226225115

Centisome position: 89.96

GC content: 40.19

Gene sequence:

>1662_bases
TTGCATAAAACAGCCAGAAAAATGGTAGTGGCACTCAGTTTATTATGTTTACTAGCACTTACAGCATGTCAGTCTGGCAA
TAAAGGGGAAAATGAAAAAGTAACATCCGACAAGAAATCCAAAGTGGTTAACATCATGGAAACGAGTGGAATTCCGAATG
CAAGTCCAACACTCGCAGACAATAGCGTAAGTTTCCGAGTAATCAATCAAATTTTTGAAGGATTATATCGATTAGATAAA
AACGGGAAACCGGCACTCGCTCTAGCTGCCAGTGAACCTGAAGAAAAAGATGGCGGGAAAACACTTATTTTTAAAATTCG
CGAAGATGCTAATTGGTCCAATGGCGACCCAGTGACGGCGCATGATTTTGAATACGCTTGGAAAAAAGTGGTGGATCCAA
AAACAGCCGCTGCATACGGACCGCAAATGGAGGAAATCGTCAAAAATGCTACCCAAATTTTAAAAGGTGAAATGTCACCG
GATGAACTTGGAATCAAAGCGTTAGACGACAAAACACTACAAATCGAACTAGAAAACCCGGTGCCAATATTCAAAGAACT
CCTAACGACGGGCACGTTCTTCCCACAAAACCAAGCGTTTGTAGAAAAACAAGGTGACCGTTATGGGACTAAAAGCGACA
CATTGATTTCCAACGGCCCGTATAAATTAGAAAATTGGAATGGAACAAATATGACATGGGATTACGTTAAAAATCCTACT
TACTGGGATAAAAAGAACGTTCCAACTAAAAAAATCCATGTAACTGTTGTAAAAGATACTAATGCGGCTTTAAACTTATA
CAATACAAATAAATTGGACCGCGTAGAGCTTGATGGTGAATTTGTTAAACAATACAAAGATGATAAAAACTTCCATAAAG
AAGCAACTGGACGCTCTGTTTATATGAAAATGAACCAAGGCAAAGATGGCGTGAAAACGGATTTAGCTAACTTAAACTTA
CGTCGGGGTTTAGCGATGGCTATCGATAAGACTGGCATGGTCAATACACTACTTGCAAATGGATCTAAAGCATTAAACGG
GGATGTTCCCGGTGATATCATGTTTGACCCAGAGACAAAAGAGGATTTCCGTAAACAAAACGGCGACTTACTCAAGTTTG
ACCAACAAGAAGCTACAAACGCATGGAAGCAAGGCTTGAAAGAAATCGGGAAAACCGAATTAACACTTACGTTAACAAGT
GGCGACACGTCCTTGCAACGCAAACAAACAGAATTTATCCAAAATCAATTAGAAGAAAATCTGCCTGGACTAACAATCAA
ATTAAAAAATGTCACAAACCAAGCGAGTTTCCAAGCAGATGTGACACAAGATTTTGAACTGTTACTAGGTGGATGGGGTG
GCGATTATCAAGATCCGCTCACATACCTCAACCTATTCTTAACAAACAGCCCGGGGAACCACACAGGCTTCTCGGATGCT
AAATACGATGAATTAGTCCTAGGAGCCAAGGGTAAACTTTCGGCAGACCCAGAAAAAAGATGGCAAGCACTACTAGAAGC
AGAACAAATTTTACTTAAAGATAACGCCGTGCTTATCCCGCTATACCAAGGTGGCCGCGCGTACTTGCAAAAAAGCAACC
TGAAAAACTACACCACGTATCCAATCGGTTCAGAAAACTACAAATGGATTGAAAAAAATTAA

Upstream 100 bases:

>100_bases
TTCGGTTTATAATGGTGGCGGAAGTTCTGCGCGAATTTGGCAGGAAGTCCGTCATTTTTTTGCGTAATCAAAAAGCGCTT
ACAAAAGAGGAGGAGAAATA

Downstream 100 bases:

>100_bases
TTTAAAAGAGTCTAGTTAACCAAACTAGGCTCTATTTTTATACCCGAAAGAAAATAAGACTTAGGATGTACCAATTTTCA
GCCTAGAGCCGTTCGTTCCT

Product: dipeptide ABC transporter (dipeptide-binding protein)

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MHKTARKMVVALSLLCLLALTACQSGNKGENEKVTSDKKSKVVNIMETSGIPNASPTLADNSVSFRVINQIFEGLYRLDK
NGKPALALAASEPEEKDGGKTLIFKIREDANWSNGDPVTAHDFEYAWKKVVDPKTAAAYGPQMEEIVKNATQILKGEMSP
DELGIKALDDKTLQIELENPVPIFKELLTTGTFFPQNQAFVEKQGDRYGTKSDTLISNGPYKLENWNGTNMTWDYVKNPT
YWDKKNVPTKKIHVTVVKDTNAALNLYNTNKLDRVELDGEFVKQYKDDKNFHKEATGRSVYMKMNQGKDGVKTDLANLNL
RRGLAMAIDKTGMVNTLLANGSKALNGDVPGDIMFDPETKEDFRKQNGDLLKFDQQEATNAWKQGLKEIGKTELTLTLTS
GDTSLQRKQTEFIQNQLEENLPGLTIKLKNVTNQASFQADVTQDFELLLGGWGGDYQDPLTYLNLFLTNSPGNHTGFSDA
KYDELVLGAKGKLSADPEKRWQALLEAEQILLKDNAVLIPLYQGGRAYLQKSNLKNYTTYPIGSENYKWIEKN

Sequences:

>Translated_553_residues
MHKTARKMVVALSLLCLLALTACQSGNKGENEKVTSDKKSKVVNIMETSGIPNASPTLADNSVSFRVINQIFEGLYRLDK
NGKPALALAASEPEEKDGGKTLIFKIREDANWSNGDPVTAHDFEYAWKKVVDPKTAAAYGPQMEEIVKNATQILKGEMSP
DELGIKALDDKTLQIELENPVPIFKELLTTGTFFPQNQAFVEKQGDRYGTKSDTLISNGPYKLENWNGTNMTWDYVKNPT
YWDKKNVPTKKIHVTVVKDTNAALNLYNTNKLDRVELDGEFVKQYKDDKNFHKEATGRSVYMKMNQGKDGVKTDLANLNL
RRGLAMAIDKTGMVNTLLANGSKALNGDVPGDIMFDPETKEDFRKQNGDLLKFDQQEATNAWKQGLKEIGKTELTLTLTS
GDTSLQRKQTEFIQNQLEENLPGLTIKLKNVTNQASFQADVTQDFELLLGGWGGDYQDPLTYLNLFLTNSPGNHTGFSDA
KYDELVLGAKGKLSADPEKRWQALLEAEQILLKDNAVLIPLYQGGRAYLQKSNLKNYTTYPIGSENYKWIEKN
>Mature_553_residues
MHKTARKMVVALSLLCLLALTACQSGNKGENEKVTSDKKSKVVNIMETSGIPNASPTLADNSVSFRVINQIFEGLYRLDK
NGKPALALAASEPEEKDGGKTLIFKIREDANWSNGDPVTAHDFEYAWKKVVDPKTAAAYGPQMEEIVKNATQILKGEMSP
DELGIKALDDKTLQIELENPVPIFKELLTTGTFFPQNQAFVEKQGDRYGTKSDTLISNGPYKLENWNGTNMTWDYVKNPT
YWDKKNVPTKKIHVTVVKDTNAALNLYNTNKLDRVELDGEFVKQYKDDKNFHKEATGRSVYMKMNQGKDGVKTDLANLNL
RRGLAMAIDKTGMVNTLLANGSKALNGDVPGDIMFDPETKEDFRKQNGDLLKFDQQEATNAWKQGLKEIGKTELTLTLTS
GDTSLQRKQTEFIQNQLEENLPGLTIKLKNVTNQASFQADVTQDFELLLGGWGGDYQDPLTYLNLFLTNSPGNHTGFSDA
KYDELVLGAKGKLSADPEKRWQALLEAEQILLKDNAVLIPLYQGGRAYLQKSNLKNYTTYPIGSENYKWIEKN

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=545, Percent_Identity=26.7889908256881, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI87081878, Length=542, Percent_Identity=27.3062730627306, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789397, Length=515, Percent_Identity=25.8252427184466, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1787762, Length=464, Percent_Identity=21.3362068965517, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61806; Mature: 61806

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKTARKMVVALSLLCLLALTACQSGNKGENEKVTSDKKSKVVNIMETSGIPNASPTLAD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCCCCCC
NSVSFRVINQIFEGLYRLDKNGKPALALAASEPEEKDGGKTLIFKIREDANWSNGDPVTA
CCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCEE
HDFEYAWKKVVDPKTAAAYGPQMEEIVKNATQILKGEMSPDELGIKALDDKTLQIELENP
CHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCCHHHCCCEEECCCEEEEEECCC
VPIFKELLTTGTFFPQNQAFVEKQGDRYGTKSDTLISNGPYKLENWNGTNMTWDYVKNPT
CHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCEECCCCEEEECCCCCCCEEHCCCCCC
YWDKKNVPTKKIHVTVVKDTNAALNLYNTNKLDRVELDGEFVKQYKDDKNFHKEATGRSV
CCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCEEECCHHHHHHHHCCCHHHHHCCCCEE
YMKMNQGKDGVKTDLANLNLRRGLAMAIDKTGMVNTLLANGSKALNGDVPGDIMFDPETK
EEEECCCCCCHHHHHHHCHHHCCEEEEECCCCCHHHHHHCCCCCCCCCCCCCEEECCCCH
EDFRKQNGDLLKFDQQEATNAWKQGLKEIGKTELTLTLTSGDTSLQRKQTEFIQNQLEEN
HHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHC
LPGLTIKLKNVTNQASFQADVTQDFELLLGGWGGDYQDPLTYLNLFLTNSPGNHTGFSDA
CCCEEEEEECCCCCCCEECCCCCCEEEEEECCCCCCCCHHEEEEEEEECCCCCCCCCCCC
KYDELVLGAKGKLSADPEKRWQALLEAEQILLKDNAVLIPLYQGGRAYLQKSNLKNYTTY
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHEEECCEEEEEEECCCHHHHHHCCCCCCEEC
PIGSENYKWIEKN
CCCCCCCEEEECC
>Mature Secondary Structure
MHKTARKMVVALSLLCLLALTACQSGNKGENEKVTSDKKSKVVNIMETSGIPNASPTLAD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCCCCCC
NSVSFRVINQIFEGLYRLDKNGKPALALAASEPEEKDGGKTLIFKIREDANWSNGDPVTA
CCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCEE
HDFEYAWKKVVDPKTAAAYGPQMEEIVKNATQILKGEMSPDELGIKALDDKTLQIELENP
CHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCCHHHCCCEEECCCEEEEEECCC
VPIFKELLTTGTFFPQNQAFVEKQGDRYGTKSDTLISNGPYKLENWNGTNMTWDYVKNPT
CHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCEECCCCEEEECCCCCCCEEHCCCCCC
YWDKKNVPTKKIHVTVVKDTNAALNLYNTNKLDRVELDGEFVKQYKDDKNFHKEATGRSV
CCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCEEECCHHHHHHHHCCCHHHHHCCCCEE
YMKMNQGKDGVKTDLANLNLRRGLAMAIDKTGMVNTLLANGSKALNGDVPGDIMFDPETK
EEEECCCCCCHHHHHHHCHHHCCEEEEECCCCCHHHHHHCCCCCCCCCCCCCEEECCCCH
EDFRKQNGDLLKFDQQEATNAWKQGLKEIGKTELTLTLTSGDTSLQRKQTEFIQNQLEEN
HHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHC
LPGLTIKLKNVTNQASFQADVTQDFELLLGGWGGDYQDPLTYLNLFLTNSPGNHTGFSDA
CCCEEEEEECCCCCCCEECCCCCCEEEEEECCCCCCCCHHEEEEEEEECCCCCCCCCCCC
KYDELVLGAKGKLSADPEKRWQALLEAEQILLKDNAVLIPLYQGGRAYLQKSNLKNYTTY
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHEEECCEEEEEEECCCHHHHHHCCCCCCEEC
PIGSENYKWIEKN
CCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]