Click here to switch to the map view.

The map label for this gene is rho

Identifier: 226225098

GI number: 226225098

Start: 2598190

End: 2599461

Strand: Reverse

Name: rho

Synonym: Lm4b_02519

Alternate gene names: 226225098

Gene position: 2599461-2598190 (Counterclockwise)

Preceding gene: 226225099

Following gene: 226225097

Centisome position: 89.25

GC content: 39.23

Gene sequence:

>1272_bases
ATGGCTAAACTGTCCATTGCATACTTAGAAAGTTTAACAATCAAAGAGATATATGCCTTAGCAAAAGAACACAAAATTAC
TTACTATAGTAAATTAACAAAACGAGAATTGATTTTTGCTTTATTAAAATCAAACGCAGAAAAAGAAGGATTTTTCTTCA
TGGAAGGTGTATTAGAAATTATTCCAAATGAAGGTTTTGGCTTCTTACGCCCTATCAACTATTCATCTAGTTCGGAAGAT
ATTTACATCTCTGCTTCCCAAATTAGACGTTTCGAGTTAAGAACTGGGGATAAGGTTTCTGGAAAAGTTCGTCCTCCGAA
AGAAAATGAACGTTACTTTGGCTTACTCCATGTTGAGGCTGTCAATGGAGAAAATCCTGAAGTGGCTAAAGAAAGAGTGC
ATTTCCCAGGACTAACACCGCTTTATCCAGATCGTCAAATTCATTTAGAAACAGGCAAAAATCCTATTTCCACAAGAACG
ATTGATTTGATTTCACCAATTGGTTTTGGTCAACGCGGACTTATTGTAGCTCCACCAAAAGCAGGTAAAACGGTATTACT
TAAAGAAATTGCGAACGCGATTACTACTAATCATCCAGAGTCAGAATTGATTGTCTTGTTAATTGATGAGCGTCCGGAAG
AAGTAACTGATATCGAACGTTCTGTAAAGGCCGATGTTGTCAGTTCCACATTTGATGAGGTACCTGAAAATCACATTAAA
GTAGCAGAACTGGTTCTTGAACGAGCGATGCGTTTAGTAGAACAAAAACGCGATGTGGTCATTTTAATGGATAGTATTAC
GCGACTTGCGAGGGCCTATAACCTAGTGATTCCGCCAAGTGGTCGAACACTTTCAGGGGGGATTGATCCTGCTGCATTCC
ATAGACCGAAGCGCTTCTTTGGAGCTGCTCGTAATATTGAAGAGGGTGGAAGCTTAACCATTCTTGCCACAGCATTAGTT
GACACGGGTTCTCGAATGGACGATGTTATTTACGAAGAATTCAAAGGGACAGGCAACATGGAGTTACATCTTGATCGCCA
ACTTGCAGAACGTCGCGTTTTTCCTGCTATCGATATGCGTCGCTCCGGCACAAGAAAAGAAGAATTACTACTATCCAAAG
AGCGTCTTGAACAACTCTGGAAAATCCGCAAAGCGATGCCGAAACAAAGCGATGGACTAGATATTTCAGAACGATTTGTA
CGTTATTTGAAGAAAACGGAGAATAATGAAGCTTTTTATGAGTTACTACAAGAAGAGATGTTTAAAAAATAA

Upstream 100 bases:

>100_bases
TGAGGCTGAACGAATCAAGTCGTTTCAGCCTTCCCTCTAAATCCCTCCATTATCATTTATTCCTAAATACTATTTATTAA
CTCAAAAAGGTGGTACATAT

Downstream 100 bases:

>100_bases
ATCAAAAATTTCACATAGAAAGAAGTTATTTTATGAGCAAAATAAGACAATTATTAAATAAAATTCCGTGGTACACAGAT
CAGGTACACAGTATTTTTAT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MAKLSIAYLESLTIKEIYALAKEHKITYYSKLTKRELIFALLKSNAEKEGFFFMEGVLEIIPNEGFGFLRPINYSSSSED
IYISASQIRRFELRTGDKVSGKVRPPKENERYFGLLHVEAVNGENPEVAKERVHFPGLTPLYPDRQIHLETGKNPISTRT
IDLISPIGFGQRGLIVAPPKAGKTVLLKEIANAITTNHPESELIVLLIDERPEEVTDIERSVKADVVSSTFDEVPENHIK
VAELVLERAMRLVEQKRDVVILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSLTILATALV
DTGSRMDDVIYEEFKGTGNMELHLDRQLAERRVFPAIDMRRSGTRKEELLLSKERLEQLWKIRKAMPKQSDGLDISERFV
RYLKKTENNEAFYELLQEEMFKK

Sequences:

>Translated_423_residues
MAKLSIAYLESLTIKEIYALAKEHKITYYSKLTKRELIFALLKSNAEKEGFFFMEGVLEIIPNEGFGFLRPINYSSSSED
IYISASQIRRFELRTGDKVSGKVRPPKENERYFGLLHVEAVNGENPEVAKERVHFPGLTPLYPDRQIHLETGKNPISTRT
IDLISPIGFGQRGLIVAPPKAGKTVLLKEIANAITTNHPESELIVLLIDERPEEVTDIERSVKADVVSSTFDEVPENHIK
VAELVLERAMRLVEQKRDVVILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSLTILATALV
DTGSRMDDVIYEEFKGTGNMELHLDRQLAERRVFPAIDMRRSGTRKEELLLSKERLEQLWKIRKAMPKQSDGLDISERFV
RYLKKTENNEAFYELLQEEMFKK
>Mature_422_residues
AKLSIAYLESLTIKEIYALAKEHKITYYSKLTKRELIFALLKSNAEKEGFFFMEGVLEIIPNEGFGFLRPINYSSSSEDI
YISASQIRRFELRTGDKVSGKVRPPKENERYFGLLHVEAVNGENPEVAKERVHFPGLTPLYPDRQIHLETGKNPISTRTI
DLISPIGFGQRGLIVAPPKAGKTVLLKEIANAITTNHPESELIVLLIDERPEEVTDIERSVKADVVSSTFDEVPENHIKV
AELVLERAMRLVEQKRDVVILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSLTILATALVD
TGSRMDDVIYEEFKGTGNMELHLDRQLAERRVFPAIDMRRSGTRKEELLLSKERLEQLWKIRKAMPKQSDGLDISERFVR
YLKKTENNEAFYELLQEEMFKK

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=419, Percent_Identity=55.3699284009546, Blast_Score=476, Evalue=1e-135,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48132; Mature: 48001

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLSIAYLESLTIKEIYALAKEHKITYYSKLTKRELIFALLKSNAEKEGFFFMEGVLEI
CCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IPNEGFGFLRPINYSSSSEDIYISASQIRRFELRTGDKVSGKVRPPKENERYFGLLHVEA
CCCCCCCCEEECCCCCCCCEEEEEHHHHHEEECCCCCCCCCCCCCCCCCCCEEEEEEEEE
VNGENPEVAKERVHFPGLTPLYPDRQIHLETGKNPISTRTIDLISPIGFGQRGLIVAPPK
ECCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCEEHHHCCCCCCCCCEEEECCC
AGKTVLLKEIANAITTNHPESELIVLLIDERPEEVTDIERSVKADVVSSTFDEVPENHIK
CCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VAELVLERAMRLVEQKRDVVILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFF
HHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHEEEECCCCCEECCCCCHHHHCCCHHHH
GAARNIEEGGSLTILATALVDTGSRMDDVIYEEFKGTGNMELHLDRQLAERRVFPAIDMR
HHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCCC
RSGTRKEELLLSKERLEQLWKIRKAMPKQSDGLDISERFVRYLKKTENNEAFYELLQEEM
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
FKK
HCC
>Mature Secondary Structure 
AKLSIAYLESLTIKEIYALAKEHKITYYSKLTKRELIFALLKSNAEKEGFFFMEGVLEI
CCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IPNEGFGFLRPINYSSSSEDIYISASQIRRFELRTGDKVSGKVRPPKENERYFGLLHVEA
CCCCCCCCEEECCCCCCCCEEEEEHHHHHEEECCCCCCCCCCCCCCCCCCCEEEEEEEEE
VNGENPEVAKERVHFPGLTPLYPDRQIHLETGKNPISTRTIDLISPIGFGQRGLIVAPPK
ECCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCEEHHHCCCCCCCCCEEEECCC
AGKTVLLKEIANAITTNHPESELIVLLIDERPEEVTDIERSVKADVVSSTFDEVPENHIK
CCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VAELVLERAMRLVEQKRDVVILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFF
HHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHEEEECCCCCEECCCCCHHHHCCCHHHH
GAARNIEEGGSLTILATALVDTGSRMDDVIYEEFKGTGNMELHLDRQLAERRVFPAIDMR
HHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCCC
RSGTRKEELLLSKERLEQLWKIRKAMPKQSDGLDISERFVRYLKKTENNEAFYELLQEEM
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
FKK
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]