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The map label for this gene is glyA

Identifier: 226225087

GI number: 226225087

Start: 2588198

End: 2589439

Strand: Reverse

Name: glyA

Synonym: Lm4b_02508

Alternate gene names: 226225087

Gene position: 2589439-2588198 (Counterclockwise)

Preceding gene: 226225088

Following gene: 226225086

Centisome position: 88.9

GC content: 39.45

Gene sequence:

>1242_bases
ATGGTCTATTTACAAAAGCAAGATAAGGAAGTTTTTGATGCAATTAAGTTAGAGCTTGGTAGACAACGCGCGAATATCGA
ATTAATCGCATCTGAGAATTTTGTTAGTGAGCAAGTAATGGAAGCTATGGGATCTGTATTAACGAATAAATATGCAGAAG
GTTACCCAGGGAAACGCTATTACGGCGGATGTGAATTCGTGGATATTGTGGAAGATTTAGCACGCGACCGCGCGAAAAAA
TTATTTGGCGCTGAATATGCGAATGTCCAACCACACTCAGGTGCTCAAGCGAATATGGCAGTTTATCATACAGTGCTTGA
ACCAGGCGATACTGTTCTTGGTATGAACCTTTCACATGGTGGGCATTTAACACATGGTAGCCCAGTAAATTTCAGCGGTG
TTTTATACAATTTTGTTGAATACGGCGTTCGTGAAGATACGAAAGAAATTGATTACGATATTGTTCGTGAAGCAGCTTTA
AAACATAAACCAAAAATGATTGTAGCTGGCGCAAGTGCTTATCCACGTAAAATTGATTTTGCTAAATTCCGCGAAATTGC
TGATGAAGTTGGCGCATACTTAATGGTAGATATGGCGCATATTGCTGGTCTTGTTGCTGCTGGCTTGCATCAAAATCCAG
TTCCTTATGCTGATTTTACAACGACAACTACCCATAAAACGCTTCGTGGACCTCGCGGCGGAATGATTTTAGCCAAAGCA
GAATGGGAACAAAAATTAAATAAATCGATTTTCCCAGGAATCCAAGGTGGACCGTTAATGCACGTAATCGCAGCGAAAGC
AGTGGCGTTTGGTGAAGCGTTACAACCTGAATTCACGGCGTACTGTGAACAAATTATTCGTAACTCTAAAAAATTAGCAG
AAACACTTCAAGCGAATGATGTTGCTGTTTTAACAGGTGGTTCGGATAATCATTTATTATTAATTGATTTAAAACCACTT
GGCTTAACAGGAAAAGCAGCGGAGAAAGTATTAGATGAAGTTGGTATTACCGTAAATAAAAATACCATTCCATTCGAAAC
GGAAAGCCCATTTGTAACAAGTGGTATCCGTGTAGGTGTTGCTGCAGTGACCACTCGTGGATTTGATGAAGTTGCGATTG
AAAAAGTTGGTGTACTTATTTCAGAAGTACTGCATAATTTAGAAAATGAAGAAGTTCTCGCAGATGTAAAAGCACGCGTG
GCTACTTTAACAAATGAATATCCGCTTTATCCAAGTTTATAA

Upstream 100 bases:

>100_bases
ATTCCAAAATGCTTGCTTTTCTACCCGATTTAAGTAATTATTAAAGATAATACATAACAGTTCACTTGAAAAATTTATCA
GAAAAGGATGGGGTCCACAG

Downstream 100 bases:

>100_bases
AATAAGTGATCCGGTTTTTTCTATAAAATAGAGAAAACCGGTTTTTTTCATGTATAGCAAATTAATCCTTGCACTTGTGT
CTTGTTCTGATACAATAAGA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MVYLQKQDKEVFDAIKLELGRQRANIELIASENFVSEQVMEAMGSVLTNKYAEGYPGKRYYGGCEFVDIVEDLARDRAKK
LFGAEYANVQPHSGAQANMAVYHTVLEPGDTVLGMNLSHGGHLTHGSPVNFSGVLYNFVEYGVREDTKEIDYDIVREAAL
KHKPKMIVAGASAYPRKIDFAKFREIADEVGAYLMVDMAHIAGLVAAGLHQNPVPYADFTTTTTHKTLRGPRGGMILAKA
EWEQKLNKSIFPGIQGGPLMHVIAAKAVAFGEALQPEFTAYCEQIIRNSKKLAETLQANDVAVLTGGSDNHLLLIDLKPL
GLTGKAAEKVLDEVGITVNKNTIPFETESPFVTSGIRVGVAAVTTRGFDEVAIEKVGVLISEVLHNLENEEVLADVKARV
ATLTNEYPLYPSL

Sequences:

>Translated_413_residues
MVYLQKQDKEVFDAIKLELGRQRANIELIASENFVSEQVMEAMGSVLTNKYAEGYPGKRYYGGCEFVDIVEDLARDRAKK
LFGAEYANVQPHSGAQANMAVYHTVLEPGDTVLGMNLSHGGHLTHGSPVNFSGVLYNFVEYGVREDTKEIDYDIVREAAL
KHKPKMIVAGASAYPRKIDFAKFREIADEVGAYLMVDMAHIAGLVAAGLHQNPVPYADFTTTTTHKTLRGPRGGMILAKA
EWEQKLNKSIFPGIQGGPLMHVIAAKAVAFGEALQPEFTAYCEQIIRNSKKLAETLQANDVAVLTGGSDNHLLLIDLKPL
GLTGKAAEKVLDEVGITVNKNTIPFETESPFVTSGIRVGVAAVTTRGFDEVAIEKVGVLISEVLHNLENEEVLADVKARV
ATLTNEYPLYPSL
>Mature_413_residues
MVYLQKQDKEVFDAIKLELGRQRANIELIASENFVSEQVMEAMGSVLTNKYAEGYPGKRYYGGCEFVDIVEDLARDRAKK
LFGAEYANVQPHSGAQANMAVYHTVLEPGDTVLGMNLSHGGHLTHGSPVNFSGVLYNFVEYGVREDTKEIDYDIVREAAL
KHKPKMIVAGASAYPRKIDFAKFREIADEVGAYLMVDMAHIAGLVAAGLHQNPVPYADFTTTTTHKTLRGPRGGMILAKA
EWEQKLNKSIFPGIQGGPLMHVIAAKAVAFGEALQPEFTAYCEQIIRNSKKLAETLQANDVAVLTGGSDNHLLLIDLKPL
GLTGKAAEKVLDEVGITVNKNTIPFETESPFVTSGIRVGVAAVTTRGFDEVAIEKVGVLISEVLHNLENEEVLADVKARV
ATLTNEYPLYPSL

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=451, Percent_Identity=40.5764966740577, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI261862352, Length=451, Percent_Identity=40.5764966740577, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI261862350, Length=451, Percent_Identity=40.5764966740577, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI261862348, Length=451, Percent_Identity=40.5764966740577, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI261862346, Length=451, Percent_Identity=39.9113082039911, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=45.3865336658354, Blast_Score=328, Evalue=4e-90,
Organism=Homo sapiens, GI22547189, Length=386, Percent_Identity=44.300518134715, Blast_Score=298, Evalue=7e-81,
Organism=Escherichia coli, GI1788902, Length=406, Percent_Identity=59.8522167487685, Blast_Score=480, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI25144732, Length=432, Percent_Identity=46.5277777777778, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25144729, Length=432, Percent_Identity=46.5277777777778, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319739, Length=431, Percent_Identity=43.6194895591647, Blast_Score=342, Evalue=7e-95,
Organism=Saccharomyces cerevisiae, GI6323087, Length=430, Percent_Identity=41.1627906976744, Blast_Score=320, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24640005, Length=452, Percent_Identity=43.141592920354, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI221329721, Length=452, Percent_Identity=43.141592920354, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45093; Mature: 45093

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYLQKQDKEVFDAIKLELGRQRANIELIASENFVSEQVMEAMGSVLTNKYAEGYPGKRY
CCEECCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
YGGCEFVDIVEDLARDRAKKLFGAEYANVQPHSGAQANMAVYHTVLEPGDTVLGMNLSHG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCC
GHLTHGSPVNFSGVLYNFVEYGVREDTKEIDYDIVREAALKHKPKMIVAGASAYPRKIDF
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCH
AKFREIADEVGAYLMVDMAHIAGLVAAGLHQNPVPYADFTTTTTHKTLRGPRGGMILAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEC
EWEQKLNKSIFPGIQGGPLMHVIAAKAVAFGEALQPEFTAYCEQIIRNSKKLAETLQAND
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHCCCC
VAVLTGGSDNHLLLIDLKPLGLTGKAAEKVLDEVGITVNKNTIPFETESPFVTSGIRVGV
EEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCCCCCCEECCHHHHH
AAVTTRGFDEVAIEKVGVLISEVLHNLENEEVLADVKARVATLTNEYPLYPSL
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVYLQKQDKEVFDAIKLELGRQRANIELIASENFVSEQVMEAMGSVLTNKYAEGYPGKRY
CCEECCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
YGGCEFVDIVEDLARDRAKKLFGAEYANVQPHSGAQANMAVYHTVLEPGDTVLGMNLSHG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCC
GHLTHGSPVNFSGVLYNFVEYGVREDTKEIDYDIVREAALKHKPKMIVAGASAYPRKIDF
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCH
AKFREIADEVGAYLMVDMAHIAGLVAAGLHQNPVPYADFTTTTTHKTLRGPRGGMILAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEC
EWEQKLNKSIFPGIQGGPLMHVIAAKAVAFGEALQPEFTAYCEQIIRNSKKLAETLQAND
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHCCCC
VAVLTGGSDNHLLLIDLKPLGLTGKAAEKVLDEVGITVNKNTIPFETESPFVTSGIRVGV
EEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCCCCCCEECCHHHHH
AAVTTRGFDEVAIEKVGVLISEVLHNLENEEVLADVKARVATLTNEYPLYPSL
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]