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The map label for this gene is ppaX [H]

Identifier: 226225029

GI number: 226225029

Start: 2529251

End: 2529904

Strand: Reverse

Name: ppaX [H]

Synonym: Lm4b_02450

Alternate gene names: 226225029

Gene position: 2529904-2529251 (Counterclockwise)

Preceding gene: 226225030

Following gene: 226225028

Centisome position: 86.86

GC content: 37.61

Gene sequence:

>654_bases
ATGACTGGGAAAATTACTACATTGTTGTTTGACTTAGATGGTACGCTTATAAATACGAATGAATTGATTATCAAAACATT
TCAAGTGACATTTCAAGAATTCATGCCGGACCGGGTTTTTACTAGAGAGGACATTTTACCGTTTATCGGGCCTTCTTTAA
TGGAGACTTTCCGGGAAATAAATCCAGCGCATGCAGATGAGATGCGCGCTTTTTACCGAGAGTATAATTTGAAGCATCAT
GATGATTTAATTTTAGAATATGATGGAGTTTATGAAGCAATTCGTGCTTTATATGAAGAAGATTATAAACTTGGAATTGT
ATCGACGAAAATGTATGATACGATTATGCGAGGTCTCAAGGTAACTGGCTTGGATAAATTTTTCCAAGTGGTGATTGGGT
TAGATCAAGTGTCTAATGCTAAACCAGATCCTGAAGGTATTGAGATGGCTCTTTCTTTATTAAATGCGACAAAAGAAGAA
GCGATTATGATTGGCGATAATTATCATGATATTGAAGCTGGAAAAAATGCGGAAACCCTAACCGCGGGTGTAGCTTGGGC
GATTAAAGGACCAGAACATTTGGCGCAGTTTCAGCCAGATTTCATGTTAGAAAAAATGAGCGATTTACTCGCAATTGTTA
GGGACGAGGAATAG

Upstream 100 bases:

>100_bases
ATGAATCCACCATATTATATGGAAGATAAATTTGGTAAAGTAGTTAAAAAGAAATAAAAAAGTTGGAGATTTTCGAGTTT
GATTAAGAAAGGAAGAAATT

Downstream 100 bases:

>100_bases
AATTTGAGGCGGCTGGATAGGTTTAAGGCACCCGATGATACAGTCAACACCCTTTTTCAAGTGTATAAAACGATTTCTTT
TTGGAAGGTTTTGAAGAATA

Product: pyrophosphatase PpaX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTGKITTLLFDLDGTLINTNELIIKTFQVTFQEFMPDRVFTREDILPFIGPSLMETFREINPAHADEMRAFYREYNLKHH
DDLILEYDGVYEAIRALYEEDYKLGIVSTKMYDTIMRGLKVTGLDKFFQVVIGLDQVSNAKPDPEGIEMALSLLNATKEE
AIMIGDNYHDIEAGKNAETLTAGVAWAIKGPEHLAQFQPDFMLEKMSDLLAIVRDEE

Sequences:

>Translated_217_residues
MTGKITTLLFDLDGTLINTNELIIKTFQVTFQEFMPDRVFTREDILPFIGPSLMETFREINPAHADEMRAFYREYNLKHH
DDLILEYDGVYEAIRALYEEDYKLGIVSTKMYDTIMRGLKVTGLDKFFQVVIGLDQVSNAKPDPEGIEMALSLLNATKEE
AIMIGDNYHDIEAGKNAETLTAGVAWAIKGPEHLAQFQPDFMLEKMSDLLAIVRDEE
>Mature_216_residues
TGKITTLLFDLDGTLINTNELIIKTFQVTFQEFMPDRVFTREDILPFIGPSLMETFREINPAHADEMRAFYREYNLKHHD
DLILEYDGVYEAIRALYEEDYKLGIVSTKMYDTIMRGLKVTGLDKFFQVVIGLDQVSNAKPDPEGIEMALSLLNATKEEA
IMIGDNYHDIEAGKNAETLTAGVAWAIKGPEHLAQFQPDFMLEKMSDLLAIVRDEE

Specific function: Hydrolyzes pyrophosphate formed during P-Ser-HPr dephosphorylation by HPrK/P. Might play a role in controlling the intracellular pyrophosphate pool [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. PpaX family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=232, Percent_Identity=24.5689655172414, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 24765; Mature: 24633

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKITTLLFDLDGTLINTNELIIKTFQVTFQEFMPDRVFTREDILPFIGPSLMETFREI
CCCCEEEEEEECCCCEECCCHHHHHHHHHHHHHHCCCHHCCHHHHHHHCCHHHHHHHHHC
NPAHADEMRAFYREYNLKHHDDLILEYDGVYEAIRALYEEDYKLGIVSTKMYDTIMRGLK
CCCCHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCC
VTGLDKFFQVVIGLDQVSNAKPDPEGIEMALSLLNATKEEAIMIGDNYHDIEAGKNAETL
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHH
TAGVAWAIKGPEHLAQFQPDFMLEKMSDLLAIVRDEE
HHCEEEEECCHHHHHHCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TGKITTLLFDLDGTLINTNELIIKTFQVTFQEFMPDRVFTREDILPFIGPSLMETFREI
CCCEEEEEEECCCCEECCCHHHHHHHHHHHHHHCCCHHCCHHHHHHHCCHHHHHHHHHC
NPAHADEMRAFYREYNLKHHDDLILEYDGVYEAIRALYEEDYKLGIVSTKMYDTIMRGLK
CCCCHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCC
VTGLDKFFQVVIGLDQVSNAKPDPEGIEMALSLLNATKEEAIMIGDNYHDIEAGKNAETL
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHH
TAGVAWAIKGPEHLAQFQPDFMLEKMSDLLAIVRDEE
HHCEEEEECCHHHHHHCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]