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The map label for this gene is sigL

Identifier: 226225009

GI number: 226225009

Start: 2506571

End: 2507914

Strand: Reverse

Name: sigL

Synonym: Lm4b_02430

Alternate gene names: 226225009

Gene position: 2507914-2506571 (Counterclockwise)

Preceding gene: 226225010

Following gene: 226225008

Centisome position: 86.1

GC content: 38.91

Gene sequence:

>1344_bases
GTGCGTTTAGAATCTAATTTCGTCCAAAAGCAACAACAAAAGCAGTCGCAGAAATTAAATATGACGCAACAATTGTCCCA
GTCTATTGCAATGCTTCAATTTGCAACGGCTGATCTAGTGGCATTTTTAGAAGATAAAGCACTCGAGAACCCATTAATTG
AAGTGATTCCAGGCTCGGATTTTGCAACAGACTTTTCTTACAGTAGTCGAAAAAGCGCTGGAAGTTCTGATGATACAGAT
TGGCTAGAACAAATAGCTGACAATAAGCTGACGTTAGCCGATGCGCTAAAAGAACAGCTACATTTAATGGATGTAACGCA
AACACAAAAAATTATCGTCTTATATTTAATAGAAAGCTTAAATGACAATGGTTATTTACAAATAGATTTGAAAGATGTCA
GTGCGGCGCTTTTAATGGATGATTCTAGTGTGTTAGAAGGCCTAGAAATCTTGCAAAGCATGGAGCCAGCCGGCGTTGGG
GCAAGAGATGCGCGTGAATGCATTTTACTACAGATCGAACGCTCGCCGGATGCTCCTTATATTGCCTATGACGTTATCCA
AAAATTCTTCACGGAATTTGCAGAGAAAAAATGGAAGAAAATCGCTGCAGAAATGGATGTTAGCTTGCAAGACATTCAAG
AAGTTTCCGACTACATTCAAACCTTAAATCCAAAGCCGGGTTCTGAATTTGAGTCTGAACGCGTTCAATATGTTGTCCCG
GATTTAATTTTACTCCAACACGAAGATGAATTTGCTGTTTCACTAGCAAAACAATTTCTGCCACAAGTGCGTTTCCAAGA
AGCATACTACGAAACGATGCGAGCAACCGAAGAAAAAGACGTCGCCCAATTTCTACGCGAAAAAGCCGGCGAATTTGATT
GGATAAAAAAAGGCATCGAACAACGCGAAAACACCTTGCAAAGAGTTGGAGAGGCGATTGTCAATCATCAGAAAGCTTAT
TTCCTAGATAATTCCGCGCATTTACAACCATTAACCTTAAAAGAAGTAGCGGAAGAGCTTGGTGTACATGAATCAACCGT
GAGCCGTGCGGTGAATGGAAAATACATGGAAACAACGAATGGTGTGTACGAATTAAAACGCTTCTTTGCATCCGGTTTAC
AAAAGAAATCTTCCGAGAACGAAAACACAGGTGATATTTCAAGTACAACTATCAAAAAGCTAGTACAAGAATTTGTAGCA
GCAGAAGATAAATTAAAACCACTCTCCGATCAAAAGATAGTAGATATGCTCGCAGAAAAAGAAATTCAAGTTTCAAGAAG
AGCTATCGCAAAGTATCGTTTAGAATTAAATATTCCTTCTTCCTCTAAAAGAAAAAGATTTTAA

Upstream 100 bases:

>100_bases
TCTGCTGTAATTTGAAAAATGGCATGTTATAATGAAGACAATAAATGGAATTTAGTTTGGTATAAGGTATATCAGACGAA
TTTTTAATGGAGGTTTTAAT

Downstream 100 bases:

>100_bases
AAGACGTTTTTTGCCTCACGAGCCGTGTGTGGGACAAAAAACGTCTTTTTAAAACAAAAAAATAACTAAGAGCATGTTTC
GTTTTTTGAAGAGGTGGAAA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MRLESNFVQKQQQKQSQKLNMTQQLSQSIAMLQFATADLVAFLEDKALENPLIEVIPGSDFATDFSYSSRKSAGSSDDTD
WLEQIADNKLTLADALKEQLHLMDVTQTQKIIVLYLIESLNDNGYLQIDLKDVSAALLMDDSSVLEGLEILQSMEPAGVG
ARDARECILLQIERSPDAPYIAYDVIQKFFTEFAEKKWKKIAAEMDVSLQDIQEVSDYIQTLNPKPGSEFESERVQYVVP
DLILLQHEDEFAVSLAKQFLPQVRFQEAYYETMRATEEKDVAQFLREKAGEFDWIKKGIEQRENTLQRVGEAIVNHQKAY
FLDNSAHLQPLTLKEVAEELGVHESTVSRAVNGKYMETTNGVYELKRFFASGLQKKSSENENTGDISSTTIKKLVQEFVA
AEDKLKPLSDQKIVDMLAEKEIQVSRRAIAKYRLELNIPSSSKRKRF

Sequences:

>Translated_447_residues
MRLESNFVQKQQQKQSQKLNMTQQLSQSIAMLQFATADLVAFLEDKALENPLIEVIPGSDFATDFSYSSRKSAGSSDDTD
WLEQIADNKLTLADALKEQLHLMDVTQTQKIIVLYLIESLNDNGYLQIDLKDVSAALLMDDSSVLEGLEILQSMEPAGVG
ARDARECILLQIERSPDAPYIAYDVIQKFFTEFAEKKWKKIAAEMDVSLQDIQEVSDYIQTLNPKPGSEFESERVQYVVP
DLILLQHEDEFAVSLAKQFLPQVRFQEAYYETMRATEEKDVAQFLREKAGEFDWIKKGIEQRENTLQRVGEAIVNHQKAY
FLDNSAHLQPLTLKEVAEELGVHESTVSRAVNGKYMETTNGVYELKRFFASGLQKKSSENENTGDISSTTIKKLVQEFVA
AEDKLKPLSDQKIVDMLAEKEIQVSRRAIAKYRLELNIPSSSKRKRF
>Mature_447_residues
MRLESNFVQKQQQKQSQKLNMTQQLSQSIAMLQFATADLVAFLEDKALENPLIEVIPGSDFATDFSYSSRKSAGSSDDTD
WLEQIADNKLTLADALKEQLHLMDVTQTQKIIVLYLIESLNDNGYLQIDLKDVSAALLMDDSSVLEGLEILQSMEPAGVG
ARDARECILLQIERSPDAPYIAYDVIQKFFTEFAEKKWKKIAAEMDVSLQDIQEVSDYIQTLNPKPGSEFESERVQYVVP
DLILLQHEDEFAVSLAKQFLPQVRFQEAYYETMRATEEKDVAQFLREKAGEFDWIKKGIEQRENTLQRVGEAIVNHQKAY
FLDNSAHLQPLTLKEVAEELGVHESTVSRAVNGKYMETTNGVYELKRFFASGLQKKSSENENTGDISSTTIKKLVQEFVA
AEDKLKPLSDQKIVDMLAEKEIQVSRRAIAKYRLELNIPSSSKRKRF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the levanase operon. The open complex (sigma-54 and core RNA poly

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=479, Percent_Identity=29.2275574112735, Blast_Score=228, Evalue=6e-61,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 50863; Mature: 50863

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00717 SIGMA54_1 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLESNFVQKQQQKQSQKLNMTQQLSQSIAMLQFATADLVAFLEDKALENPLIEVIPGSD
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
FATDFSYSSRKSAGSSDDTDWLEQIADNKLTLADALKEQLHLMDVTQTQKIIVLYLIESL
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NDNGYLQIDLKDVSAALLMDDSSVLEGLEILQSMEPAGVGARDARECILLQIERSPDAPY
CCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHCCCEEEEEEECCCCCCC
IAYDVIQKFFTEFAEKKWKKIAAEMDVSLQDIQEVSDYIQTLNPKPGSEFESERVQYVVP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
DLILLQHEDEFAVSLAKQFLPQVRFQEAYYETMRATEEKDVAQFLREKAGEFDWIKKGIE
HHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHH
QRENTLQRVGEAIVNHQKAYFLDNSAHLQPLTLKEVAEELGVHESTVSRAVNGKYMETTN
HHHHHHHHHHHHHHCCCHHEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCEEECCC
GVYELKRFFASGLQKKSSENENTGDISSTTIKKLVQEFVAAEDKLKPLSDQKIVDMLAEK
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EIQVSRRAIAKYRLELNIPSSSKRKRF
HHHHHHHHHHHHEEEEECCCCCCCCCC
>Mature Secondary Structure
MRLESNFVQKQQQKQSQKLNMTQQLSQSIAMLQFATADLVAFLEDKALENPLIEVIPGSD
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
FATDFSYSSRKSAGSSDDTDWLEQIADNKLTLADALKEQLHLMDVTQTQKIIVLYLIESL
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NDNGYLQIDLKDVSAALLMDDSSVLEGLEILQSMEPAGVGARDARECILLQIERSPDAPY
CCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHCCCEEEEEEECCCCCCC
IAYDVIQKFFTEFAEKKWKKIAAEMDVSLQDIQEVSDYIQTLNPKPGSEFESERVQYVVP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
DLILLQHEDEFAVSLAKQFLPQVRFQEAYYETMRATEEKDVAQFLREKAGEFDWIKKGIE
HHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHH
QRENTLQRVGEAIVNHQKAYFLDNSAHLQPLTLKEVAEELGVHESTVSRAVNGKYMETTN
HHHHHHHHHHHHHHCCCHHEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCEEECCC
GVYELKRFFASGLQKKSSENENTGDISSTTIKKLVQEFVAAEDKLKPLSDQKIVDMLAEK
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EIQVSRRAIAKYRLELNIPSSSKRKRF
HHHHHHHHHHHHEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1924373; 8969506; 9384377 [H]