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The map label for this gene is gadB [C]

Identifier: 226224981

GI number: 226224981

Start: 2473851

End: 2475254

Strand: Reverse

Name: gadB [C]

Synonym: Lm4b_02402

Alternate gene names: 226224981

Gene position: 2475254-2473851 (Counterclockwise)

Preceding gene: 226224983

Following gene: 226224978

Centisome position: 84.98

GC content: 39.6

Gene sequence:

>1404_bases
ATGCTTTATAGTGAAGACGACAAGCGAAAACAGGAAAGTTATCGAATCCCACTCTTTGGATCTGAGGAAGAAAGCACGAG
TATCCCGAAATATGTATTAAAAAAGGAACCGATGGAACCACGTATTGCGTACCAACTAGTGAAAGACCAGTTGATGGATG
AGGGAAATGCGCGGCAAAACTTAGCAACTTTTTGTCAGACATACATGGAAAAAGAGGCAGAAATACTGATGGCAGAAACG
CTCGAGAAAAATGCAATTGATAAATCCGAATATCCGCAAACAGCTGAACTGGAAAATCGCTGCGTCAATATTTTAGCCGA
TTTGTGGAATGCTCCAAAAGATATGTCTTACTTGGGGACTTCGACAGTAGGCTCTTCGGAAGCGTGTATGCTTGGCGGTT
TGGCAATGAAATTTCGCTGGCGTAACCATGCGGAAAAACGAGGTCTAGATATTCAAGCGAAACGACCGAACTTAATTATT
TCATCAGGCTACCAAGTATGTTGGGAAAAATTCTGTGTGTACTGGGATGTAGATATGCGTGTTGTGCCAATGGATAAAGA
GCATCTCAGTATCGATGTAGATAAAGTGTTTGATTTGGTTGATGAATACACAATTGGGATTGTCGGGATTTTAGGCATTA
CTTATACTGGTAAATTTGATGATATTCAATTATTAGATGAAAAAGTAGAAGCATATAATGAAGCGAACGAGCATCAATTA
GTCATTCATGTTGACGGAGCAAGTGGCGCGATGTTTACACCATTTGTAAATCCGGAATTACCTTGGGATTTCCGCCTTAA
AAATGTTGTTTCTATAAATACTTCCGGTCATAAATACGGATTGGTTTATCCTGGCGTTGGTTGGATTTTATGGAAAGACA
AAGAATATTTACCAAAAGAGCTTATATTTGAAGTCAGCTACTTAGGTGGTTCGATGCCGACGATGGCGATTAACTTCTCT
CGTAGTGCCAGTCAAATTATTGGTCAATATTATAATTTTTTACGATATGGTTTTGAAGGTTACCGAGAAATTCATGAAAA
AACAAAGAAAACAGCCCTTTACTTGGCAAAAAGTGTAGAAAAAAGTGGCTACTTTGAAATTATTAATGATGGCGCTAATT
TACCGATTGTTTGTTACAAAATGAAAGAAGGACTGGATGTAGAGTGGACGCTATATGACTTGGCTGATCAGCTTTTAATG
AAAGGATGGCAAGTTCCAGCCTATCCACTTCCGGCCGATTTAAGTGATACGATTATTCAACGGTTTGTTTGTCGGGCGGA
CTTAGGCTATAACGTGGCTGAAGAGTTTGCGGTTGATTTTGCAGACGCGCTTCATAATCTGGAACATGCTAGAGTGCTGT
ATCATGACAAAGAACGCAACGACTCATACGGATTTACGCACTAA

Upstream 100 bases:

>100_bases
TAGTATAACAAATTATTGTGAAAAATTGAACGGTTTGTCTCTAAGGTTTAATGGGTATTGGTGAGAGAAGATGATCAGTT
ATTTTAAAGGAGGAAAAAAG

Downstream 100 bases:

>100_bases
AAAAGAAGACTTGGATGGTGAGTCCGAGTCTTCTTTTTATTAAAGTGGAAGCCAGTCGATAATTTCTTTGATACTCTTAT
CCCAAAAATCCCAATCATGG

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-gamma [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MLYSEDDKRKQESYRIPLFGSEEESTSIPKYVLKKEPMEPRIAYQLVKDQLMDEGNARQNLATFCQTYMEKEAEILMAET
LEKNAIDKSEYPQTAELENRCVNILADLWNAPKDMSYLGTSTVGSSEACMLGGLAMKFRWRNHAEKRGLDIQAKRPNLII
SSGYQVCWEKFCVYWDVDMRVVPMDKEHLSIDVDKVFDLVDEYTIGIVGILGITYTGKFDDIQLLDEKVEAYNEANEHQL
VIHVDGASGAMFTPFVNPELPWDFRLKNVVSINTSGHKYGLVYPGVGWILWKDKEYLPKELIFEVSYLGGSMPTMAINFS
RSASQIIGQYYNFLRYGFEGYREIHEKTKKTALYLAKSVEKSGYFEIINDGANLPIVCYKMKEGLDVEWTLYDLADQLLM
KGWQVPAYPLPADLSDTIIQRFVCRADLGYNVAEEFAVDFADALHNLEHARVLYHDKERNDSYGFTH

Sequences:

>Translated_467_residues
MLYSEDDKRKQESYRIPLFGSEEESTSIPKYVLKKEPMEPRIAYQLVKDQLMDEGNARQNLATFCQTYMEKEAEILMAET
LEKNAIDKSEYPQTAELENRCVNILADLWNAPKDMSYLGTSTVGSSEACMLGGLAMKFRWRNHAEKRGLDIQAKRPNLII
SSGYQVCWEKFCVYWDVDMRVVPMDKEHLSIDVDKVFDLVDEYTIGIVGILGITYTGKFDDIQLLDEKVEAYNEANEHQL
VIHVDGASGAMFTPFVNPELPWDFRLKNVVSINTSGHKYGLVYPGVGWILWKDKEYLPKELIFEVSYLGGSMPTMAINFS
RSASQIIGQYYNFLRYGFEGYREIHEKTKKTALYLAKSVEKSGYFEIINDGANLPIVCYKMKEGLDVEWTLYDLADQLLM
KGWQVPAYPLPADLSDTIIQRFVCRADLGYNVAEEFAVDFADALHNLEHARVLYHDKERNDSYGFTH
>Mature_467_residues
MLYSEDDKRKQESYRIPLFGSEEESTSIPKYVLKKEPMEPRIAYQLVKDQLMDEGNARQNLATFCQTYMEKEAEILMAET
LEKNAIDKSEYPQTAELENRCVNILADLWNAPKDMSYLGTSTVGSSEACMLGGLAMKFRWRNHAEKRGLDIQAKRPNLII
SSGYQVCWEKFCVYWDVDMRVVPMDKEHLSIDVDKVFDLVDEYTIGIVGILGITYTGKFDDIQLLDEKVEAYNEANEHQL
VIHVDGASGAMFTPFVNPELPWDFRLKNVVSINTSGHKYGLVYPGVGWILWKDKEYLPKELIFEVSYLGGSMPTMAINFS
RSASQIIGQYYNFLRYGFEGYREIHEKTKKTALYLAKSVEKSGYFEIINDGANLPIVCYKMKEGLDVEWTLYDLADQLLM
KGWQVPAYPLPADLSDTIIQRFVCRADLGYNVAEEFAVDFADALHNLEHARVLYHDKERNDSYGFTH

Specific function: Catalyzes The Production Of Gaba. [C]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=337, Percent_Identity=23.4421364985163, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1787769, Length=456, Percent_Identity=43.421052631579, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1789934, Length=440, Percent_Identity=44.0909090909091, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17557272, Length=331, Percent_Identity=25.0755287009063, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17543922, Length=403, Percent_Identity=22.0843672456576, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25148342, Length=384, Percent_Identity=22.1354166666667, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323905, Length=475, Percent_Identity=36.8421052631579, Blast_Score=290, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6320500, Length=336, Percent_Identity=23.2142857142857, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355963, Length=363, Percent_Identity=25.068870523416, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24654344, Length=363, Percent_Identity=25.068870523416, Blast_Score=109, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 53663; Mature: 53663

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYSEDDKRKQESYRIPLFGSEEESTSIPKYVLKKEPMEPRIAYQLVKDQLMDEGNARQN
CCCCCCCHHHHHCEECCEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHH
LATFCQTYMEKEAEILMAETLEKNAIDKSEYPQTAELENRCVNILADLWNAPKDMSYLGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCC
STVGSSEACMLGGLAMKFRWRNHAEKRGLDIQAKRPNLIISSGYQVCWEKFCVYWDVDMR
CCCCCCCHHHHCCCCEEEEHHCCHHHCCCCEEECCCCEEEECCHHHHHHHEEEEEECCEE
VVPMDKEHLSIDVDKVFDLVDEYTIGIVGILGITYTGKFDDIQLLDEKVEAYNEANEHQL
EEECCHHHCEEEHHHHHHHHHHHHHHHHEEEHEEECCCCCHHHHHHHHHHHHCCCCCCEE
VIHVDGASGAMFTPFVNPELPWDFRLKNVVSINTSGHKYGLVYPGVGWILWKDKEYLPKE
EEEEECCCCCEECCCCCCCCCCCEEECEEEEECCCCCEEEEEECCCEEEEECCCHHCCHH
LIFEVSYLGGSMPTMAINFSRSASQIIGQYYNFLRYGFEGYREIHEKTKKTALYLAKSVE
HHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KSGYFEIINDGANLPIVCYKMKEGLDVEWTLYDLADQLLMKGWQVPAYPLPADLSDTIIQ
CCCCEEEEECCCCCCEEEEEECCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RFVCRADLGYNVAEEFAVDFADALHNLEHARVLYHDKERNDSYGFTH
HHHHHHCCCCCHHHHHHHHHHHHHHCHHHHEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MLYSEDDKRKQESYRIPLFGSEEESTSIPKYVLKKEPMEPRIAYQLVKDQLMDEGNARQN
CCCCCCCHHHHHCEECCEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHH
LATFCQTYMEKEAEILMAETLEKNAIDKSEYPQTAELENRCVNILADLWNAPKDMSYLGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCC
STVGSSEACMLGGLAMKFRWRNHAEKRGLDIQAKRPNLIISSGYQVCWEKFCVYWDVDMR
CCCCCCCHHHHCCCCEEEEHHCCHHHCCCCEEECCCCEEEECCHHHHHHHEEEEEECCEE
VVPMDKEHLSIDVDKVFDLVDEYTIGIVGILGITYTGKFDDIQLLDEKVEAYNEANEHQL
EEECCHHHCEEEHHHHHHHHHHHHHHHHEEEHEEECCCCCHHHHHHHHHHHHCCCCCCEE
VIHVDGASGAMFTPFVNPELPWDFRLKNVVSINTSGHKYGLVYPGVGWILWKDKEYLPKE
EEEEECCCCCEECCCCCCCCCCCEEECEEEEECCCCCEEEEEECCCEEEEECCCHHCCHH
LIFEVSYLGGSMPTMAINFSRSASQIIGQYYNFLRYGFEGYREIHEKTKKTALYLAKSVE
HHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KSGYFEIINDGANLPIVCYKMKEGLDVEWTLYDLADQLLMKGWQVPAYPLPADLSDTIIQ
CCCCEEEEECCCCCCEEEEEECCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RFVCRADLGYNVAEEFAVDFADALHNLEHARVLYHDKERNDSYGFTH
HHHHHHCCCCCHHHHHHHHHHHHHHCHHHHEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]