Click here to switch to the map view.

The map label for this gene is lacE [H]

Identifier: 226224910

GI number: 226224910

Start: 2396882

End: 2398183

Strand: Reverse

Name: lacE [H]

Synonym: Lm4b_02329

Alternate gene names: 226224910

Gene position: 2398183-2396882 (Counterclockwise)

Preceding gene: 226224911

Following gene: 226224909

Centisome position: 82.34

GC content: 37.4

Gene sequence:

>1302_bases
ATGAAAGCATTAGAACGATTTCTGTCAAAATGGTTATTACCATTTGCAAAAGTACTAGAAAGTAACACCCAGATGAAGGC
AGTACGTCAAGGAATGATGGCACTTGTACCAATTACTTTAGTTGGTGCAGTTCCCGTTTTATTCCAACAGCTAGGCGGGA
TTCCGAAACTACCTAGTTGGATTGCAGCGGTTTCCAATTATATCAATAACATCACTTCTCCAATATATTTTGCCACTTTT
GGATTAATGTCCGTATATGTGGCGGTTTTTGTAGCATATTACTATGCGAAGGAACGCAATTTATGGGATATCGGGGCGAT
TGTAACAGCCTTAATGAGTTTTGTTGTCGTTGCAGTAAGACCACTTGAATCAGGCGGGAGCGATGTAGCGTATCTAGCCG
GTGAAGGGATTTTTGTTGCTTTAGTAATTAGTTTATTGTCTGTCGAAATTTTACACATTTTTAAGAATAAGTTGAAATTC
ACGATTAATTTAGGACAAGGTGTTCCAACGCCGATTTTACGTTCTTTTGAAAATTTATGGCCAATTTTATTCTCTGTTTT
AATTATTGCTATTTTAAGTTTTGGGATAGAAACATTAAGTGGTATTCGTGTAGTTGAGTTAATTCAAACACTATTTTCTC
CACTGACATCCCTAGTAAACACATTACCAGGTATTATGTTAATTATCTTTATCCAACAATTATTATGGTGGTTTGGTATT
CATGGCTATTCTGTTATGGCGCCAGTTTGGCTCAGTGTAGCGTTCCAAAACGTGGATGCGAATGCAGCAGCTCTTGCAAA
AGGAGAACCACTTTCTTCTATGCTGATTTTCACTCCTGACTTTATGTGGAGTATTGTAGGTGTAACTGGGGCTGGGGTTA
CAGGAGCACTCGTTGTCATTATGATGTTCTCCAAATCAAAACGATATAAAACACTTGGTAGACTTGCTTTAATTCCAACC
TTCTTTAGTATTAATGAGCCAGTAATGTTTGGTGTTCCAATTGTGTTAAATCCACGCTTCTTTATTCCGATGATGCTTGC
ACCGCAAATTGCTGCTTTGATTGGTTGGTTCTCTATCAAGCTTGGATTAATGAATCCATTTACGATGGTTTCTCCATATG
TACCTGTTCCAATCGGTGCTATTGTCGCTTCATTTGACTGGAGATATGTTATTGTACTTGGTCTTATCTTGGTCTGTTCG
GCCCTTATTTACTATCCATTCTTTAAAATTGCTGAAAAAGAAGCTATTTTACAAGAAACGAGTGGAGACCAAGAAGCTAG
TTTAGATGATTTTGATTTTTAA

Upstream 100 bases:

>100_bases
GTGACAATTTCCTATGCTTAGGAAATAAGATTAATTGAGGGTGAATTTTGTATGCGCTATCATGAACGTATAACAGCTTA
GGGAAAGGAAATGAAAATCA

Downstream 100 bases:

>100_bases
GTAGGAAAGGACGATAGACATGAAAATTATGTTAGTATGTTTTGGCGGATTATCCACTTCCATTTTAGTAAAAAAGATGG
AAGAGGCGATTGCGGCATCT

Product: PTS system,cellobiose-specific enzyme IIC component

Products: NA

Alternate protein names: EIICB-Lac; EII-Lac; Lactose permease IIC component; PTS system lactose-specific EIIC component; Lactose-specific phosphotransferase enzyme IIB component; PTS system lactose-specific EIIB component [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKALERFLSKWLLPFAKVLESNTQMKAVRQGMMALVPITLVGAVPVLFQQLGGIPKLPSWIAAVSNYINNITSPIYFATF
GLMSVYVAVFVAYYYAKERNLWDIGAIVTALMSFVVVAVRPLESGGSDVAYLAGEGIFVALVISLLSVEILHIFKNKLKF
TINLGQGVPTPILRSFENLWPILFSVLIIAILSFGIETLSGIRVVELIQTLFSPLTSLVNTLPGIMLIIFIQQLLWWFGI
HGYSVMAPVWLSVAFQNVDANAAALAKGEPLSSMLIFTPDFMWSIVGVTGAGVTGALVVIMMFSKSKRYKTLGRLALIPT
FFSINEPVMFGVPIVLNPRFFIPMMLAPQIAALIGWFSIKLGLMNPFTMVSPYVPVPIGAIVASFDWRYVIVLGLILVCS
ALIYYPFFKIAEKEAILQETSGDQEASLDDFDF

Sequences:

>Translated_433_residues
MKALERFLSKWLLPFAKVLESNTQMKAVRQGMMALVPITLVGAVPVLFQQLGGIPKLPSWIAAVSNYINNITSPIYFATF
GLMSVYVAVFVAYYYAKERNLWDIGAIVTALMSFVVVAVRPLESGGSDVAYLAGEGIFVALVISLLSVEILHIFKNKLKF
TINLGQGVPTPILRSFENLWPILFSVLIIAILSFGIETLSGIRVVELIQTLFSPLTSLVNTLPGIMLIIFIQQLLWWFGI
HGYSVMAPVWLSVAFQNVDANAAALAKGEPLSSMLIFTPDFMWSIVGVTGAGVTGALVVIMMFSKSKRYKTLGRLALIPT
FFSINEPVMFGVPIVLNPRFFIPMMLAPQIAALIGWFSIKLGLMNPFTMVSPYVPVPIGAIVASFDWRYVIVLGLILVCS
ALIYYPFFKIAEKEAILQETSGDQEASLDDFDF
>Mature_433_residues
MKALERFLSKWLLPFAKVLESNTQMKAVRQGMMALVPITLVGAVPVLFQQLGGIPKLPSWIAAVSNYINNITSPIYFATF
GLMSVYVAVFVAYYYAKERNLWDIGAIVTALMSFVVVAVRPLESGGSDVAYLAGEGIFVALVISLLSVEILHIFKNKLKF
TINLGQGVPTPILRSFENLWPILFSVLIIAILSFGIETLSGIRVVELIQTLFSPLTSLVNTLPGIMLIIFIQQLLWWFGI
HGYSVMAPVWLSVAFQNVDANAAALAKGEPLSSMLIFTPDFMWSIVGVTGAGVTGALVVIMMFSKSKRYKTLGRLALIPT
FFSINEPVMFGVPIVLNPRFFIPMMLAPQIAALIGWFSIKLGLMNPFTMVSPYVPVPIGAIVASFDWRYVIVLGLILVCS
ALIYYPFFKIAEKEAILQETSGDQEASLDDFDF

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=450, Percent_Identity=28.6666666666667, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014350
- InterPro:   IPR003501
- InterPro:   IPR013012
- InterPro:   IPR003352
- InterPro:   IPR004501
- InterPro:   IPR004801 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 47707; Mature: 47707

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1400164; 8277252; 12397186 [H]