Click here to switch to the map view.

The map label for this gene is psuG [H]

Identifier: 226224885

GI number: 226224885

Start: 2373135

End: 2374046

Strand: Reverse

Name: psuG [H]

Synonym: Lm4b_02304

Alternate gene names: 226224885

Gene position: 2374046-2373135 (Counterclockwise)

Preceding gene: 226224886

Following gene: 226224884

Centisome position: 81.51

GC content: 40.57

Gene sequence:

>912_bases
ATGAAAAATTATTTATCATTATCCGAAGAAGTAAAACAAGCAAAAGCAGAAGGAAAAGCAATCGTTGCGCTAGAATCTAC
AATTATCTCTCACGGCATGCCTTATCCGCAAAATGTTGAAATGGCACGTGACGTAGAACAAATTATTCGCGATAACGGTG
CCGTACCAGCGACAATCGCTTTAATCGACGGAAAAATTAAAATTGGTCTTTCTGATGAAGAACTAGAACTATTCGCTAAA
AGTAGCAATGTGGCAAAAGTTTCTCGTCGTGATATCGGTTACCTTATTGCAACAAAACAACTTGGCGCAACAACTGTAGC
AGCAACAATGATTTGTGCTGAACTCGCAGAAATCGGCATCTTCGTAACTGGTGGGATTGGCGGCGTTCACCGTGGTGCCG
AAACAACAATGGACGTTTCAGCTGACTTAGAAGAATTAGCGAAAACAAATGTTGCTGTCGTTTGCGCAGGAGCTAAATCA
ATCCTAGACCTAAACTTAACAATGGAATACTTAGAAACAAAAGGGGTTCCAGTTATTGGTTACCAAACCGACGTATTACC
AGCATTTTACACGCGTTCAAGCGATGTAGAATTAACATTGCGTGCAGATGCTCCAGAAGTTATCGCAGAATCGCTTAAAG
CGAAATGGGATCTTCAAATCGAAGGTGGCGCAGTAATTACTAACCCAATCCCAGAAGAATTCGCTATGGACGAAAAAGTA
ATTAACGATGTAATCCAAACAGCGCTTAAAGAAGCTGAAGAAAATCACATTCACGGGAAAGACGTTACACCATTCCTACT
TGGTAAAGTAAAAGAACTAACAGATGGTAAGAGCCTCGAAGCAAATATCGAGCTTGTAAAACACAATGCTTTAATCGGTA
CGCAAATCGCCGTAGCATACCAAAATATCTAA

Upstream 100 bases:

>100_bases
GAGCAGGGAGCGTAAATGCCGCGCGAACACTCGAATCTGAGTATACCGTTCGCCAAAATTTATCGACATCCCAACTACAA
AAAGACATGGAGGAATTCAA

Downstream 100 bases:

>100_bases
TAATCTCAGAGCTGTCGCCTATAAGTTGGGGGCAGCTTTTTTATGTTAAAATAAATGTGAATATAAAGGAGGAAAATAAA
ATGATTGCAGTAAGTGCTTG

Product: indigoidine synthase A-like protein (IndA)

Products: NA

Alternate protein names: PsiMP glycosidase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKNYLSLSEEVKQAKAEGKAIVALESTIISHGMPYPQNVEMARDVEQIIRDNGAVPATIALIDGKIKIGLSDEELELFAK
SSNVAKVSRRDIGYLIATKQLGATTVAATMICAELAEIGIFVTGGIGGVHRGAETTMDVSADLEELAKTNVAVVCAGAKS
ILDLNLTMEYLETKGVPVIGYQTDVLPAFYTRSSDVELTLRADAPEVIAESLKAKWDLQIEGGAVITNPIPEEFAMDEKV
INDVIQTALKEAEENHIHGKDVTPFLLGKVKELTDGKSLEANIELVKHNALIGTQIAVAYQNI

Sequences:

>Translated_303_residues
MKNYLSLSEEVKQAKAEGKAIVALESTIISHGMPYPQNVEMARDVEQIIRDNGAVPATIALIDGKIKIGLSDEELELFAK
SSNVAKVSRRDIGYLIATKQLGATTVAATMICAELAEIGIFVTGGIGGVHRGAETTMDVSADLEELAKTNVAVVCAGAKS
ILDLNLTMEYLETKGVPVIGYQTDVLPAFYTRSSDVELTLRADAPEVIAESLKAKWDLQIEGGAVITNPIPEEFAMDEKV
INDVIQTALKEAEENHIHGKDVTPFLLGKVKELTDGKSLEANIELVKHNALIGTQIAVAYQNI
>Mature_303_residues
MKNYLSLSEEVKQAKAEGKAIVALESTIISHGMPYPQNVEMARDVEQIIRDNGAVPATIALIDGKIKIGLSDEELELFAK
SSNVAKVSRRDIGYLIATKQLGATTVAATMICAELAEIGIFVTGGIGGVHRGAETTMDVSADLEELAKTNVAVVCAGAKS
ILDLNLTMEYLETKGVPVIGYQTDVLPAFYTRSSDVELTLRADAPEVIAESLKAKWDLQIEGGAVITNPIPEEFAMDEKV
INDVIQTALKEAEENHIHGKDVTPFLLGKVKELTDGKSLEANIELVKHNALIGTQIAVAYQNI

Specific function: Catalyzes the hydrolysis of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil [H]

COG id: COG2313

COG function: function code Q; Uncharacterized enzyme involved in pigment biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine-5'-phosphate glycosidase family [H]

Homologues:

Organism=Escherichia coli, GI1788490, Length=300, Percent_Identity=58, Blast_Score=348, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI25147470, Length=298, Percent_Identity=49.6644295302013, Blast_Score=292, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24580669, Length=305, Percent_Identity=48.1967213114754, Blast_Score=282, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022830
- InterPro:   IPR007342 [H]

Pfam domain/function: PF04227 Indigoidine_A [H]

EC number: NA

Molecular weight: Translated: 32559; Mature: 32559

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYLSLSEEVKQAKAEGKAIVALESTIISHGMPYPQNVEMARDVEQIIRDNGAVPATIA
CCCHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEE
LIDGKIKIGLSDEELELFAKSSNVAKVSRRDIGYLIATKQLGATTVAATMICAELAEIGI
EEECEEEECCCHHHHHEEECCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCE
FVTGGIGGVHRGAETTMDVSADLEELAKTNVAVVCAGAKSILDLNLTMEYLETKGVPVIG
EEECCCCCCCCCCCCEEECHHHHHHHHHCCEEEEEECCCCEEEEHEEHHHHHHCCCEEEE
YQTDVLPAFYTRSSDVELTLRADAPEVIAESLKAKWDLQIEGGAVITNPIPEEFAMDEKV
ECCCCCHHHCCCCCCEEEEEECCCHHHHHHHHCCEEEEEEECCEEEECCCCHHHHHHHHH
INDVIQTALKEAEENHIHGKDVTPFLLGKVKELTDGKSLEANIELVKHNALIGTQIAVAY
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEECCEECCEEEEEE
QNI
ECC
>Mature Secondary Structure
MKNYLSLSEEVKQAKAEGKAIVALESTIISHGMPYPQNVEMARDVEQIIRDNGAVPATIA
CCCHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEE
LIDGKIKIGLSDEELELFAKSSNVAKVSRRDIGYLIATKQLGATTVAATMICAELAEIGI
EEECEEEECCCHHHHHEEECCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCE
FVTGGIGGVHRGAETTMDVSADLEELAKTNVAVVCAGAKSILDLNLTMEYLETKGVPVIG
EEECCCCCCCCCCCCEEECHHHHHHHHHCCEEEEEECCCCEEEEHEEHHHHHHCCCEEEE
YQTDVLPAFYTRSSDVELTLRADAPEVIAESLKAKWDLQIEGGAVITNPIPEEFAMDEKV
ECCCCCHHHCCCCCCEEEEEECCCHHHHHHHHCCEEEEEEECCEEEECCCCHHHHHHHHH
INDVIQTALKEAEENHIHGKDVTPFLLGKVKELTDGKSLEANIELVKHNALIGTQIAVAY
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEECCEECCEEEEEE
QNI
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA