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The map label for this gene is fruA

Identifier: 226224880

GI number: 226224880

Start: 2367499

End: 2369397

Strand: Reverse

Name: fruA

Synonym: Lm4b_02299

Alternate gene names: 226224880

Gene position: 2369397-2367499 (Counterclockwise)

Preceding gene: 226224881

Following gene: 226224879

Centisome position: 81.35

GC content: 39.49

Gene sequence:

>1899_bases
ATGAGAATCACTGATTTATTAAGCAAAGATGTTATGATTATGTCATTACAGGCAACGACAAAAGAAGCTGCCATCGATGA
AATGATTGCTTCGTTAAAGAGCAACGGGAAAATTAATGATGAGGTGCTGTTTAAAGAAGCTATAATGAACCGTGAAGCAC
AGAGTTCTACTGGTGTAGGTGAAGGAATTGCAATGCCTCACGCGAAAACAAAAGCAGTAAATGAACCAACTGTTGTTTTT
GCTAAGAGTGAAAAAGGATTGGATTATGATGCTTTAGATGGTCAGCCAGCACACTTATTCTTTATGATTGCTGCTCCAGA
TGGCGCTAATGCAACTCATTTAGAAACTTTAGCAGCTCTTTCTAGATTATTGGTTCATCCAGCGTTTGTGCAATCGTTAA
GAGATGCTAAAACTCCAGAGGAAGTAATCAATTTATTCAACAATGAACAAGAAGACGCAGAAGAAACAGTCGTTGCTCCA
ACCTCAACCAATGAAACTGGTAAATCAGTCGTAGCAGTTACAGCATGTCCAACAGGAATTGCACATACTTATATGGCTGC
AGAAAAATTACAAGAAACTGCTAATAAATTAGGTGTACGAATCAAAGTTGAGACAAATGGCTCTAGAGGCGTTGAAAACC
GTTTAACAGATAAAGAAATTGCAGAAGCTGATGGAGTTATTATTGCAGCTGACGTACAAGTAGATATGCCTCGTTTTGAC
GGGAAGCATTTAATTGCTAAACCAGTTGCAGCGGGAATTCATAAACCAGAAGAACTAATTAAAGAAGCAATTTCAGGCAA
TGCTCCAATTTATAAAGCAGAGGAAGGTAGTCAAGCTACGGAGAGCGCAGACGGACTTTCTATCGGTCAACAAATTTATA
AACATTTAATGAGCGGTGTTTCTCACATGTTGCCGTTTGTAATTGGTGGAGGTATCGCTATCGCCATCGCGTTTATGCTT
GATCAAATGTTAGGTGTTCCTCAAGATCAACTCGCAAAACTTGGTTCATATAACGAAATCCCAGCACTATTAAAACAAAT
CGGTGATGTTGCATTCGGCTTCATGCTTCCAGTATTCGCTGGATATATTGCCTACAGTATATCGGATAGACCAGGACTTG
TAGCAGGTTTTGTTGCAGGTGGAGTAGCATCTGTTGGTGGTGCAGGCTTCCTTGGAGCACTTGTTGGTGGTTTCCTTGCA
GGTTATGCAGTAGAGTTAATTAAACTAGCACTTAAAAAGTTACCAAAAACATTAGACGGTATCAAAGTAGTTTTATTCTA
TCCAGTTTTATCGGTGTTAATTGTCGGACTTTTAATGTTGCTATTAAATGTACCAATGAGCGCATTGAACACTTGGTTGA
ATGACTTCTTGAATAGTTTAAGTGGAACGAACGCAGTTATTTTAGGATTACTGTTAGGTGCAATGATGGCAGCGGATTTA
GGTGGACCAATTAATAAAGCTGCTTATATATTTGCAACGGGTACACTCGCAGCTAGTGTAGCAACAGGCGGTAGCGCAAT
CATGGCAGCAACAATGGCTGCTGGCATGGTACCACCACTAGCAACGTTTGTAGCAACTCTTATTTTTAGAAATAAATTTA
CAGCTCAAGAACGTGATGCAGGCTTAACTAACTCTATTTTGGGTGCTTCATTCATTACTGAAGGAGCTATTCCATTTGCA
GCGGCCGATCCACTTCGAATGATTCCGAGTTTTATTGCCGGAAGTGCTATCACAGGTGCGGTAGTAATGTTCTTAAACAT
TAAAGTATTAGCACCTCACGGAGGAGTTTTTGTTATCTTCCTTGTATCCCAACCTTGGTTCTATATTATTGCAATTATTA
TTGGAACATTAATCAGCGCAGGATTAATTGGTGTTCTACGTAAGAAACCAACTGTATAA

Upstream 100 bases:

>100_bases
GGTGGAGCTACTGCATTTTCAACAGATTTAGCCCAAAAAGAACTAATAGATGAACTATTACCGCAAGTGAAGATAACCAA
AATAACAGGGAGGAACTAAA

Downstream 100 bases:

>100_bases
AATATGGAAATCGTCTTTTGAAACTAAAAAGGCGATTTCTTTTTAGTAATTTCTCGAATTATTTCCGAAAAATTGCTATA
CTGGAAGTGAAGTGAGGTGG

Product: phosphotransferase system (PTS) fructose-specific enzyme IIABC component

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MRITDLLSKDVMIMSLQATTKEAAIDEMIASLKSNGKINDEVLFKEAIMNREAQSSTGVGEGIAMPHAKTKAVNEPTVVF
AKSEKGLDYDALDGQPAHLFFMIAAPDGANATHLETLAALSRLLVHPAFVQSLRDAKTPEEVINLFNNEQEDAEETVVAP
TSTNETGKSVVAVTACPTGIAHTYMAAEKLQETANKLGVRIKVETNGSRGVENRLTDKEIAEADGVIIAADVQVDMPRFD
GKHLIAKPVAAGIHKPEELIKEAISGNAPIYKAEEGSQATESADGLSIGQQIYKHLMSGVSHMLPFVIGGGIAIAIAFML
DQMLGVPQDQLAKLGSYNEIPALLKQIGDVAFGFMLPVFAGYIAYSISDRPGLVAGFVAGGVASVGGAGFLGALVGGFLA
GYAVELIKLALKKLPKTLDGIKVVLFYPVLSVLIVGLLMLLLNVPMSALNTWLNDFLNSLSGTNAVILGLLLGAMMAADL
GGPINKAAYIFATGTLAASVATGGSAIMAATMAAGMVPPLATFVATLIFRNKFTAQERDAGLTNSILGASFITEGAIPFA
AADPLRMIPSFIAGSAITGAVVMFLNIKVLAPHGGVFVIFLVSQPWFYIIAIIIGTLISAGLIGVLRKKPTV

Sequences:

>Translated_632_residues
MRITDLLSKDVMIMSLQATTKEAAIDEMIASLKSNGKINDEVLFKEAIMNREAQSSTGVGEGIAMPHAKTKAVNEPTVVF
AKSEKGLDYDALDGQPAHLFFMIAAPDGANATHLETLAALSRLLVHPAFVQSLRDAKTPEEVINLFNNEQEDAEETVVAP
TSTNETGKSVVAVTACPTGIAHTYMAAEKLQETANKLGVRIKVETNGSRGVENRLTDKEIAEADGVIIAADVQVDMPRFD
GKHLIAKPVAAGIHKPEELIKEAISGNAPIYKAEEGSQATESADGLSIGQQIYKHLMSGVSHMLPFVIGGGIAIAIAFML
DQMLGVPQDQLAKLGSYNEIPALLKQIGDVAFGFMLPVFAGYIAYSISDRPGLVAGFVAGGVASVGGAGFLGALVGGFLA
GYAVELIKLALKKLPKTLDGIKVVLFYPVLSVLIVGLLMLLLNVPMSALNTWLNDFLNSLSGTNAVILGLLLGAMMAADL
GGPINKAAYIFATGTLAASVATGGSAIMAATMAAGMVPPLATFVATLIFRNKFTAQERDAGLTNSILGASFITEGAIPFA
AADPLRMIPSFIAGSAITGAVVMFLNIKVLAPHGGVFVIFLVSQPWFYIIAIIIGTLISAGLIGVLRKKPTV
>Mature_632_residues
MRITDLLSKDVMIMSLQATTKEAAIDEMIASLKSNGKINDEVLFKEAIMNREAQSSTGVGEGIAMPHAKTKAVNEPTVVF
AKSEKGLDYDALDGQPAHLFFMIAAPDGANATHLETLAALSRLLVHPAFVQSLRDAKTPEEVINLFNNEQEDAEETVVAP
TSTNETGKSVVAVTACPTGIAHTYMAAEKLQETANKLGVRIKVETNGSRGVENRLTDKEIAEADGVIIAADVQVDMPRFD
GKHLIAKPVAAGIHKPEELIKEAISGNAPIYKAEEGSQATESADGLSIGQQIYKHLMSGVSHMLPFVIGGGIAIAIAFML
DQMLGVPQDQLAKLGSYNEIPALLKQIGDVAFGFMLPVFAGYIAYSISDRPGLVAGFVAGGVASVGGAGFLGALVGGFLA
GYAVELIKLALKKLPKTLDGIKVVLFYPVLSVLIVGLLMLLLNVPMSALNTWLNDFLNSLSGTNAVILGLLLGAMMAADL
GGPINKAAYIFATGTLAASVATGGSAIMAATMAAGMVPPLATFVATLIFRNKFTAQERDAGLTNSILGASFITEGAIPFA
AADPLRMIPSFIAGSAITGAVVMFLNIKVLAPHGGVFVIFLVSQPWFYIIAIIIGTLISAGLIGVLRKKPTV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=470, Percent_Identity=44.2553191489362, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1786951, Length=644, Percent_Identity=35.5590062111801, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI87082348, Length=480, Percent_Identity=36.6666666666667, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1790386, Length=321, Percent_Identity=38.006230529595, Blast_Score=182, Evalue=7e-47,
Organism=Escherichia coli, GI1788729, Length=415, Percent_Identity=28.433734939759, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1790387, Length=92, Percent_Identity=42.3913043478261, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI2367327, Length=141, Percent_Identity=26.241134751773, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789597, Length=157, Percent_Identity=29.9363057324841, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788730, Length=97, Percent_Identity=39.1752577319588, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1790390, Length=94, Percent_Identity=39.3617021276596, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 66201; Mature: 66201

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITDLLSKDVMIMSLQATTKEAAIDEMIASLKSNGKINDEVLFKEAIMNREAQSSTGVG
CCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCC
EGIAMPHAKTKAVNEPTVVFAKSEKGLDYDALDGQPAHLFFMIAAPDGANATHLETLAAL
CCCCCCCHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHH
SRLLVHPAFVQSLRDAKTPEEVINLFNNEQEDAEETVVAPTSTNETGKSVVAVTACPTGI
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHEEECCCCCCCCCCEEEEEEECCCHH
AHTYMAAEKLQETANKLGVRIKVETNGSRGVENRLTDKEIAEADGVIIAADVQVDMPRFD
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHCCCHHHHHHHCCEEEEEECEEECCCCC
GKHLIAKPVAAGIHKPEELIKEAISGNAPIYKAEEGSQATESADGLSIGQQIYKHLMSGV
CCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCCCCCHHHCCCHHHHHHHHHHHHHHHH
SHMLPFVIGGGIAIAIAFMLDQMLGVPQDQLAKLGSYNEIPALLKQIGDVAFGFMLPVFA
HHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GYIAYSISDRPGLVAGFVAGGVASVGGAGFLGALVGGFLAGYAVELIKLALKKLPKTLDG
HHHHEECCCCCCHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IKVVLFYPVLSVLIVGLLMLLLNVPMSALNTWLNDFLNSLSGTNAVILGLLLGAMMAADL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
GGPINKAAYIFATGTLAASVATGGSAIMAATMAAGMVPPLATFVATLIFRNKFTAQERDA
CCCCCCCEEEEEECHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHCC
GLTNSILGASFITEGAIPFAAADPLRMIPSFIAGSAITGAVVMFLNIKVLAPHGGVFVIF
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHEEEEECCCCCEEEEE
LVSQPWFYIIAIIIGTLISAGLIGVLRKKPTV
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRITDLLSKDVMIMSLQATTKEAAIDEMIASLKSNGKINDEVLFKEAIMNREAQSSTGVG
CCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCC
EGIAMPHAKTKAVNEPTVVFAKSEKGLDYDALDGQPAHLFFMIAAPDGANATHLETLAAL
CCCCCCCHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHH
SRLLVHPAFVQSLRDAKTPEEVINLFNNEQEDAEETVVAPTSTNETGKSVVAVTACPTGI
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHEEECCCCCCCCCCEEEEEEECCCHH
AHTYMAAEKLQETANKLGVRIKVETNGSRGVENRLTDKEIAEADGVIIAADVQVDMPRFD
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHCCCHHHHHHHCCEEEEEECEEECCCCC
GKHLIAKPVAAGIHKPEELIKEAISGNAPIYKAEEGSQATESADGLSIGQQIYKHLMSGV
CCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCCCCCHHHCCCHHHHHHHHHHHHHHHH
SHMLPFVIGGGIAIAIAFMLDQMLGVPQDQLAKLGSYNEIPALLKQIGDVAFGFMLPVFA
HHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GYIAYSISDRPGLVAGFVAGGVASVGGAGFLGALVGGFLAGYAVELIKLALKKLPKTLDG
HHHHEECCCCCCHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IKVVLFYPVLSVLIVGLLMLLLNVPMSALNTWLNDFLNSLSGTNAVILGLLLGAMMAADL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
GGPINKAAYIFATGTLAASVATGGSAIMAATMAAGMVPPLATFVATLIFRNKFTAQERDA
CCCCCCCEEEEEECHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHCC
GLTNSILGASFITEGAIPFAAADPLRMIPSFIAGSAITGAVVMFLNIKVLAPHGGVFVIF
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHEEEEECCCCCEEEEE
LVSQPWFYIIAIIIGTLISAGLIGVLRKKPTV
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]