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The map label for this gene is pbpF [H]

Identifier: 226224837

GI number: 226224837

Start: 2323893

End: 2326037

Strand: Reverse

Name: pbpF [H]

Synonym: Lm4b_02256

Alternate gene names: 226224837

Gene position: 2326037-2323893 (Counterclockwise)

Preceding gene: 226224841

Following gene: 226224836

Centisome position: 79.86

GC content: 38.6

Gene sequence:

>2145_bases
ATGGACAAATTCAAACAGCAACTAATTAAATACTTAAAATTATTCTTTGGTTTTATAGGAAAATGGCTTAGCAAAGGATG
GCAGAAATTTAGACGTTTCTGGAAAAACAAACATATCGGAAAGATTTTTTTACTAGCTGGACTTGTATTTTTATTAAGTT
TTATCATTTATCTTGTAATAGTAGCAAAATCTGCCGACATAGATGCACTAAAAAAAGGCTTAGAGTCAGCCACGATTATT
TATGACAAAGATGGCGACAAGGCAGGGGAGCTTTCTTCCACTGATGCAACATTTGTATCCATCGATAAAATATCTAAAAA
CTTACAAAATGCAGTAGTTTCCATTGAAGATAGAAAATTTTATGAACATAAAGGTTTTGACTTGAAAGGGATAGCTCGGG
CGGGTGTTAATTTAGTCACTAGCGGAGGCATTTCTGGTGGCGGTAGTACGATTACACAGCAACTCGCCAAAAATGCTCTA
CTGACACAAGAACAAACCTTCACACGAAAAGCCAAAGAAATATTTATGGCTCGTGAAATCGAAAAAACCTACTCAAAAGA
CGAAATTATGGAAATGTACTTAAACCGTTCTTACTTTGGTAATGGGGAATGGGGAGTAGAAAATGCTTCCTTAAAATATT
TTGGTAAATCAGCGGCAGACTTAAACATACCAGAAGCTGCGACAATTGCTGGATTGCTTCAAGCTCCAAGTGCGTATGAT
CCATACCAACATATCGACAAAGCGACTAATCGCCGCAACATGGTATTAAATGCAATGGTCGAAACAGGCACAATTTCAAA
AGCAGAAGGCGATAAATACAAAGCTACAAAAATAGTTTTAAATGATCAATCAAAAGATCCACTTGCGAATAAATATCCAT
GGTACGTCGATGCAGTTATTAATGAGGCTGTAAATGAAGCAGATATAACGCAAGATGAAATCATGCAAAAAGGATATAAA
ATCTATACGGAATTAGATCAAAATTATCAAACTTCCTTAGAAAATGTTTACAACAATGATGGGTTATTCCCTTCTAATGC
AAATGATGGCACACTAGTTCAATCAGGAGCTGTTTTAATGGATCCAGCTACTGGAGGCATTAGAGCGCTTGTTGGTGGAC
GCGGAGAACATGTCTTCCGTGGTTTCAACCGGGCGACACAGATGAAAGCACAACCAGGTTCCACAATGAAGCCACTTGCG
GTATACACACCGGCGCTTCAATCTGGTTATGACGTGGATTCAATGCTAAAAGATGAAAAAATAACCTACAAAGGTAACTA
CACACCAACCAATGTTGGCGGCGTTTATAGTGGAGAAGTTCCAATGTATAAAGCTGTAGCCAACTCGATTAATGCCCCTG
CCGTTTGGTTACTTGATCAAATTGGTATTGATAAAGGTGTAAAATCTGTCGAAAAATTTGGTATTACAGTTCCAGAAAAA
GATAGAACGCTTGGACTTGCACTCGGTGGTATGAGTAAAGGTGCTTCGCCAGTAGAAATGGCAACAGCGTATGCAACCTT
TGCTAACAATGGTGCCAAACCGGAATCTCATATTATTACTAAAATTGTCGATCCATCAGGCAATACAGTTTACGAAAATG
TTCCTAAAACTAAACAAATCATTTCAGAAACAGTATCTAATGAAATGACTTCCATGCTTTTAGATGTTATCAATACAGGA
ACTGGTCAAAGTGCAGCTGTTTCCGGTCACGAAATGGCTGGGAAAACAGGTTCCACGCAAGTTCCATTTGATGATACAAG
CGGTACTAAAGATCAATGGTTTGTTGGCTACACGCCAAACCTAGTCGGAGCCGTTTGGATGGGATATGATAAAACTGACA
AAGAGCATTACCTAACTACAACCAGTTCTGCTGGTGTTTCTAGTTTGGCTCACTACGTAATGAATAGTGGTTTGCAGTAT
CAGAAATCCGCAGACTTTAGTACGAAGAGTGCTGCACAAGAAACTGCAGCGAAAAAAGAAGAAGAAGAAAAAGAGAAGAA
CAGTGGTAGCGACTTTTGGAGTGGCGTGAAAGAAAAAGCCGATGAAGCTGGAGAAACAATCAAAAAAGGCGCAGATAAAG
TAAAAGAATTCGGTGGAAAAGTTTCTGATGGCATTGGAAACTTAATCGACTCGATAGGTAATTAA

Upstream 100 bases:

>100_bases
CGGTCTAAAGTTGGTGGCGTAAAGTTGCTTTTTAACGTAAAATACGTGTATAACTGTGTGTATAGTTTATTTAATACCGT
GTAGAAGATAGGAGAGTTAC

Downstream 100 bases:

>100_bases
ATTCAAAGCACATTTTACTATGTTAACTCTAGTAAAATGTGCTTTTTTGTATTGCATTAATATTATAACTGTTATACAAT
AAGTATAACAAAAGAATGAG

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MDKFKQQLIKYLKLFFGFIGKWLSKGWQKFRRFWKNKHIGKIFLLAGLVFLLSFIIYLVIVAKSADIDALKKGLESATII
YDKDGDKAGELSSTDATFVSIDKISKNLQNAVVSIEDRKFYEHKGFDLKGIARAGVNLVTSGGISGGGSTITQQLAKNAL
LTQEQTFTRKAKEIFMAREIEKTYSKDEIMEMYLNRSYFGNGEWGVENASLKYFGKSAADLNIPEAATIAGLLQAPSAYD
PYQHIDKATNRRNMVLNAMVETGTISKAEGDKYKATKIVLNDQSKDPLANKYPWYVDAVINEAVNEADITQDEIMQKGYK
IYTELDQNYQTSLENVYNNDGLFPSNANDGTLVQSGAVLMDPATGGIRALVGGRGEHVFRGFNRATQMKAQPGSTMKPLA
VYTPALQSGYDVDSMLKDEKITYKGNYTPTNVGGVYSGEVPMYKAVANSINAPAVWLLDQIGIDKGVKSVEKFGITVPEK
DRTLGLALGGMSKGASPVEMATAYATFANNGAKPESHIITKIVDPSGNTVYENVPKTKQIISETVSNEMTSMLLDVINTG
TGQSAAVSGHEMAGKTGSTQVPFDDTSGTKDQWFVGYTPNLVGAVWMGYDKTDKEHYLTTTSSAGVSSLAHYVMNSGLQY
QKSADFSTKSAAQETAAKKEEEEKEKNSGSDFWSGVKEKADEAGETIKKGADKVKEFGGKVSDGIGNLIDSIGN

Sequences:

>Translated_714_residues
MDKFKQQLIKYLKLFFGFIGKWLSKGWQKFRRFWKNKHIGKIFLLAGLVFLLSFIIYLVIVAKSADIDALKKGLESATII
YDKDGDKAGELSSTDATFVSIDKISKNLQNAVVSIEDRKFYEHKGFDLKGIARAGVNLVTSGGISGGGSTITQQLAKNAL
LTQEQTFTRKAKEIFMAREIEKTYSKDEIMEMYLNRSYFGNGEWGVENASLKYFGKSAADLNIPEAATIAGLLQAPSAYD
PYQHIDKATNRRNMVLNAMVETGTISKAEGDKYKATKIVLNDQSKDPLANKYPWYVDAVINEAVNEADITQDEIMQKGYK
IYTELDQNYQTSLENVYNNDGLFPSNANDGTLVQSGAVLMDPATGGIRALVGGRGEHVFRGFNRATQMKAQPGSTMKPLA
VYTPALQSGYDVDSMLKDEKITYKGNYTPTNVGGVYSGEVPMYKAVANSINAPAVWLLDQIGIDKGVKSVEKFGITVPEK
DRTLGLALGGMSKGASPVEMATAYATFANNGAKPESHIITKIVDPSGNTVYENVPKTKQIISETVSNEMTSMLLDVINTG
TGQSAAVSGHEMAGKTGSTQVPFDDTSGTKDQWFVGYTPNLVGAVWMGYDKTDKEHYLTTTSSAGVSSLAHYVMNSGLQY
QKSADFSTKSAAQETAAKKEEEEKEKNSGSDFWSGVKEKADEAGETIKKGADKVKEFGGKVSDGIGNLIDSIGN
>Mature_714_residues
MDKFKQQLIKYLKLFFGFIGKWLSKGWQKFRRFWKNKHIGKIFLLAGLVFLLSFIIYLVIVAKSADIDALKKGLESATII
YDKDGDKAGELSSTDATFVSIDKISKNLQNAVVSIEDRKFYEHKGFDLKGIARAGVNLVTSGGISGGGSTITQQLAKNAL
LTQEQTFTRKAKEIFMAREIEKTYSKDEIMEMYLNRSYFGNGEWGVENASLKYFGKSAADLNIPEAATIAGLLQAPSAYD
PYQHIDKATNRRNMVLNAMVETGTISKAEGDKYKATKIVLNDQSKDPLANKYPWYVDAVINEAVNEADITQDEIMQKGYK
IYTELDQNYQTSLENVYNNDGLFPSNANDGTLVQSGAVLMDPATGGIRALVGGRGEHVFRGFNRATQMKAQPGSTMKPLA
VYTPALQSGYDVDSMLKDEKITYKGNYTPTNVGGVYSGEVPMYKAVANSINAPAVWLLDQIGIDKGVKSVEKFGITVPEK
DRTLGLALGGMSKGASPVEMATAYATFANNGAKPESHIITKIVDPSGNTVYENVPKTKQIISETVSNEMTSMLLDVINTG
TGQSAAVSGHEMAGKTGSTQVPFDDTSGTKDQWFVGYTPNLVGAVWMGYDKTDKEHYLTTTSSAGVSSLAHYVMNSGLQY
QKSADFSTKSAAQETAAKKEEEEKEKNSGSDFWSGVKEKADEAGETIKKGADKVKEFGGKVSDGIGNLIDSIGN

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=529, Percent_Identity=29.3005671077505, Blast_Score=192, Evalue=9e-50,
Organism=Escherichia coli, GI87082258, Length=315, Percent_Identity=35.2380952380952, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1788867, Length=551, Percent_Identity=27.5862068965517, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1789601, Length=166, Percent_Identity=36.7469879518072, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 77829; Mature: 77829

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFKQQLIKYLKLFFGFIGKWLSKGWQKFRRFWKNKHIGKIFLLAGLVFLLSFIIYLVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VAKSADIDALKKGLESATIIYDKDGDKAGELSSTDATFVSIDKISKNLQNAVVSIEDRKF
HHCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHEEECCCHH
YEHKGFDLKGIARAGVNLVTSGGISGGGSTITQQLAKNALLTQEQTFTRKAKEIFMAREI
HHHCCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKTYSKDEIMEMYLNRSYFGNGEWGVENASLKYFGKSAADLNIPEAATIAGLLQAPSAYD
HHHHCHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHCCCCCC
PYQHIDKATNRRNMVLNAMVETGTISKAEGDKYKATKIVLNDQSKDPLANKYPWYVDAVI
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHH
NEAVNEADITQDEIMQKGYKIYTELDQNYQTSLENVYNNDGLFPSNANDGTLVQSGAVLM
HHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCEEEE
DPATGGIRALVGGRGEHVFRGFNRATQMKAQPGSTMKPLAVYTPALQSGYDVDSMLKDEK
CCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHEECHHHHCCCCHHHHHCCCC
ITYKGNYTPTNVGGVYSGEVPMYKAVANSINAPAVWLLDQIGIDKGVKSVEKFGITVPEK
EEEECCCCCCCCCCEECCCCCHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCC
DRTLGLALGGMSKGASPVEMATAYATFANNGAKPESHIITKIVDPSGNTVYENVPKTKQI
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCEECCCCCHHHHH
ISETVSNEMTSMLLDVINTGTGQSAAVSGHEMAGKTGSTQVPFDDTSGTKDQWFVGYTPN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEECCCH
LVGAVWMGYDKTDKEHYLTTTSSAGVSSLAHYVMNSGLQYQKSADFSTKSAAQETAAKKE
HHHHHEECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
EEEKEKNSGSDFWSGVKEKADEAGETIKKGADKVKEFGGKVSDGIGNLIDSIGN
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKFKQQLIKYLKLFFGFIGKWLSKGWQKFRRFWKNKHIGKIFLLAGLVFLLSFIIYLVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VAKSADIDALKKGLESATIIYDKDGDKAGELSSTDATFVSIDKISKNLQNAVVSIEDRKF
HHCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHEEECCCHH
YEHKGFDLKGIARAGVNLVTSGGISGGGSTITQQLAKNALLTQEQTFTRKAKEIFMAREI
HHHCCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKTYSKDEIMEMYLNRSYFGNGEWGVENASLKYFGKSAADLNIPEAATIAGLLQAPSAYD
HHHHCHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHCCCCCC
PYQHIDKATNRRNMVLNAMVETGTISKAEGDKYKATKIVLNDQSKDPLANKYPWYVDAVI
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHH
NEAVNEADITQDEIMQKGYKIYTELDQNYQTSLENVYNNDGLFPSNANDGTLVQSGAVLM
HHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCEEEE
DPATGGIRALVGGRGEHVFRGFNRATQMKAQPGSTMKPLAVYTPALQSGYDVDSMLKDEK
CCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHEECHHHHCCCCHHHHHCCCC
ITYKGNYTPTNVGGVYSGEVPMYKAVANSINAPAVWLLDQIGIDKGVKSVEKFGITVPEK
EEEECCCCCCCCCCEECCCCCHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCC
DRTLGLALGGMSKGASPVEMATAYATFANNGAKPESHIITKIVDPSGNTVYENVPKTKQI
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCEECCCCCHHHHH
ISETVSNEMTSMLLDVINTGTGQSAAVSGHEMAGKTGSTQVPFDDTSGTKDQWFVGYTPN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEECCCH
LVGAVWMGYDKTDKEHYLTTTSSAGVSSLAHYVMNSGLQYQKSADFSTKSAAQETAAKKE
HHHHHEECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
EEEKEKNSGSDFWSGVKEKADEAGETIKKGADKVKEFGGKVSDGIGNLIDSIGN
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]