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The map label for this gene is glcR [H]

Identifier: 226224713

GI number: 226224713

Start: 2187297

End: 2188058

Strand: Direct

Name: glcR [H]

Synonym: Lm4b_02129

Alternate gene names: 226224713

Gene position: 2187297-2188058 (Clockwise)

Preceding gene: 226224711

Following gene: 226224714

Centisome position: 75.1

GC content: 35.7

Gene sequence:

>762_bases
ATGAACCAAAAAGAACGAATAGTGGAGGAACTGAAGCTGCTAGAGAACAAGAAAACTATCTCTCAGGAAGAACTCATGCA
GATTTTTTCCATTTCAAAAGATACAGCAAGGCGCGATATTTTAAAATTAGTCGAAAGCGGACTCGCAGAACGCTATCCAG
GTGGCGTGTCACTACCAGTTTTAAAACCTCAAATAGAAAGCTATACAAGTAGATTAGTAAAACAAAGTGAACAGAAAAAG
AAAATTGCTTATGCAGCAGGTAAAGTGATAAAAGATCATATGACAATCTATTTAGATGTTTCGACCACTGTTCATTTTTT
GGCTTCAAACCTTGAGCAACATGATTTAGTAGTAGTAACGAATTCGATGGATAATGCCATAGCTGCATCTCAGAAAGAAG
CGAACAAAGTATATTTGCTCGGTGGTTTTTTCAACTTCCATTCGCGTGTATTATCCGGTGAGCCCGTTTTAGGTCAACTT
CAGCAATTTAACTTCGATGTTGCTTTTATTGGAGGGGCTGGACTAACGGAAGATGGCATCTTTTATTCAGAACTTACAGA
TGTTTACATGAAACAAGAGATTATCAGGAATTCCGAAAAAGTATTTTTACTAATTGATAGTACAAAAGTAAATAAAAAGA
CAGCTTCCAAAATTGATTTTTCTGGAATTGATGCCGTAATAACAGATCAGCCTTTACCAAAAGATTTAATGCAGTGCCTT
ATTTCTAAAGAAGTAGAAGTTATATCTTTGAAAGGATTGTGA

Upstream 100 bases:

>100_bases
ATCAGGTTGACATAAACTTGAAAAGGTATATAATTAACTTATAAAAACTTATTTATATAAAAAATAAACTTAAGATAAAT
TATCTAAGGTGGCGAAAATG

Downstream 100 bases:

>100_bases
AAATAATGGCCGTTATCAAAAATGTGAAAATTTACAAAGAAAATGAATTAATTGATGCAGCAGTTATTACAGAAGGAAAT
TTGATAAAAGAAGTTTGCTC

Product: transcriptional regulator (DeoR family)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNQKERIVEELKLLENKKTISQEELMQIFSISKDTARRDILKLVESGLAERYPGGVSLPVLKPQIESYTSRLVKQSEQKK
KIAYAAGKVIKDHMTIYLDVSTTVHFLASNLEQHDLVVVTNSMDNAIAASQKEANKVYLLGGFFNFHSRVLSGEPVLGQL
QQFNFDVAFIGGAGLTEDGIFYSELTDVYMKQEIIRNSEKVFLLIDSTKVNKKTASKIDFSGIDAVITDQPLPKDLMQCL
ISKEVEVISLKGL

Sequences:

>Translated_253_residues
MNQKERIVEELKLLENKKTISQEELMQIFSISKDTARRDILKLVESGLAERYPGGVSLPVLKPQIESYTSRLVKQSEQKK
KIAYAAGKVIKDHMTIYLDVSTTVHFLASNLEQHDLVVVTNSMDNAIAASQKEANKVYLLGGFFNFHSRVLSGEPVLGQL
QQFNFDVAFIGGAGLTEDGIFYSELTDVYMKQEIIRNSEKVFLLIDSTKVNKKTASKIDFSGIDAVITDQPLPKDLMQCL
ISKEVEVISLKGL
>Mature_253_residues
MNQKERIVEELKLLENKKTISQEELMQIFSISKDTARRDILKLVESGLAERYPGGVSLPVLKPQIESYTSRLVKQSEQKK
KIAYAAGKVIKDHMTIYLDVSTTVHFLASNLEQHDLVVVTNSMDNAIAASQKEANKVYLLGGFFNFHSRVLSGEPVLGQL
QQFNFDVAFIGGAGLTEDGIFYSELTDVYMKQEIIRNSEKVFLLIDSTKVNKKTASKIDFSGIDAVITDQPLPKDLMQCL
ISKEVEVISLKGL

Specific function: Plays a role in carbon catabolite repression (CCR). Specifically required for transcriptional repression of the levanase operon by glucose but not by other sugars [H]

COG id: COG1349

COG function: function code KG; Transcriptional regulators of sugar metabolism

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH deoR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789829, Length=253, Percent_Identity=31.6205533596838, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1789519, Length=254, Percent_Identity=25.9842519685039, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87082344, Length=243, Percent_Identity=27.5720164609054, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788069, Length=236, Percent_Identity=30.0847457627119, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789059, Length=254, Percent_Identity=28.3464566929134, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI226510968, Length=245, Percent_Identity=27.7551020408163, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790635, Length=250, Percent_Identity=23.6, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790753, Length=246, Percent_Identity=25.2032520325203, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014036
- InterPro:   IPR001034
- InterPro:   IPR018356
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00455 DeoR; PF08220 HTH_DeoR [H]

EC number: NA

Molecular weight: Translated: 28319; Mature: 28319

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS51000 HTH_DEOR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKERIVEELKLLENKKTISQEELMQIFSISKDTARRDILKLVESGLAERYPGGVSLPV
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LKPQIESYTSRLVKQSEQKKKIAYAAGKVIKDHMTIYLDVSTTVHFLASNLEQHDLVVVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHCCCCCCEEEEE
NSMDNAIAASQKEANKVYLLGGFFNFHSRVLSGEPVLGQLQQFNFDVAFIGGAGLTEDGI
CCCCCHHHHCCCCCCEEEEEECHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCCCC
FYSELTDVYMKQEIIRNSEKVFLLIDSTKVNKKTASKIDFSGIDAVITDQPLPKDLMQCL
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCCCCEEECCCCCCHHHHHHH
ISKEVEVISLKGL
HHCCCEEEEECCC
>Mature Secondary Structure
MNQKERIVEELKLLENKKTISQEELMQIFSISKDTARRDILKLVESGLAERYPGGVSLPV
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LKPQIESYTSRLVKQSEQKKKIAYAAGKVIKDHMTIYLDVSTTVHFLASNLEQHDLVVVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHCCCCCCEEEEE
NSMDNAIAASQKEANKVYLLGGFFNFHSRVLSGEPVLGQLQQFNFDVAFIGGAGLTEDGI
CCCCCHHHHCCCCCCEEEEEECHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCCCC
FYSELTDVYMKQEIIRNSEKVFLLIDSTKVNKKTASKIDFSGIDAVITDQPLPKDLMQCL
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCCCCEEECCCCCCHHHHHHH
ISKEVEVISLKGL
HHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]