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The map label for this gene is argG

Identifier: 226224696

GI number: 226224696

Start: 2168948

End: 2170162

Strand: Direct

Name: argG

Synonym: Lm4b_02111

Alternate gene names: 226224696

Gene position: 2168948-2170162 (Clockwise)

Preceding gene: 226224680

Following gene: 226224697

Centisome position: 74.47

GC content: 39.01

Gene sequence:

>1215_bases
ATGGCGAAAGAAAAAATCGTATTAGCTTACTCAGGTGGGTTAGATACTTCTGTGGCAATTCAGTGGTTAGTAGAATCAGG
TTATGAAGTTATTGCATGTTGCTTAGATGTTGGTGAAGGCAAAAATTTGGATTTTATTAAAGAAAAAGCCATTACTGTTG
GAGCAAGTGAATCCTACACGATTGATGCAAAAGAAGAATTCGCGGAGGATTTTGCGTTAATTGCCCTTCAAGCCCACGCA
TATTATGAAGGAAAATATCCGCTTATTTCCGCTTTAAGTCGTCCGTTAATTGCGAAAAAATTAGTAGAAGTAGCTCGTCA
AGAAGGTGCCTCTGCTATCGCTCATGGCTGTACTGGTAAAGGAAATGACCAAGTTCGGTTTGAAGTAGCGATTCATGCAC
TTGCTCCTGATTTAAAAGTTGTTTCCCCTGTCCGTGATTGGAAATGGTCTAGAGAAGAAGAAATCAATTACGCCAAAGAA
CATAACATCCCTGTTCCAATTGATTTAGATAATCCCTTCTCGATTGACCAAAACCTTTGGGGTAGAAGTAACGAATGTGG
GGTGCTTGAAAACCCATGGACAACACCTCCAGAAGCAGCTTACGACTTAACAGTGAGTTTAGAAGACGCACCAGATACAC
CAGATATTGTTGAAATTACGTTTGATGCCGGTATTCCAATTTCTCTAAACGGAGAAAATATGAGCCTAGCAAATTTGATC
CTTACTTTAAATGAAATTGCTGGAAAACATGGTGTCGGTAGAATCGATCATATCGAAAATCGTTTAGTTGGCATTAAATC
ACGTGAAGTATATGAATGTCCGGCCGCAGTCACTTTGATTACTGCACATAAAGAATTAGAAGATTTAACTTTTGTTCGTG
AAGTGGCACATTTCAAACCTATTATCGAACAAAAAATCAGCGAAACAATTTACAATGGCCTATGGTTCTCACCTTTGACA
GAAGCATTAGTTGCTTTCTTAAAATCCACGCAAAAATTCGTGAACGGAACGATTCGTGTAAAACTGTTTAAAGGCCATGC
CATTGTGGAAGGAAGAAAATCACCAAATTCGCTTTATGATGAAAACCTAGCGACTTACACGTCTTCCGATACATTTGACC
AAGATGCAGCAGTTGGTTTCATCAAGCTTTGGGGATTACCAACGAAAGTAAGCGCAGAAGTTAATTCAAAAGTGACAATA
ACAACTGAGGTGTAA

Upstream 100 bases:

>100_bases
ATAATTATGAATAATTCAAAATAAAAAGGTTGATATATAAAAGCATCGGTGTTAATATAAATACAAATGTTTGTATATTT
ATGAATAGGGGATGTTTACA

Downstream 100 bases:

>100_bases
AAAATGGAAAAACTATGGGGCGGACGTTTTCAAGGGAAAAGTGAAGCATGGATTGACGATTTTGGTGCTTCGATTTCTTT
TGATCAAAAAATGGCAAAAG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MAKEKIVLAYSGGLDTSVAIQWLVESGYEVIACCLDVGEGKNLDFIKEKAITVGASESYTIDAKEEFAEDFALIALQAHA
YYEGKYPLISALSRPLIAKKLVEVARQEGASAIAHGCTGKGNDQVRFEVAIHALAPDLKVVSPVRDWKWSREEEINYAKE
HNIPVPIDLDNPFSIDQNLWGRSNECGVLENPWTTPPEAAYDLTVSLEDAPDTPDIVEITFDAGIPISLNGENMSLANLI
LTLNEIAGKHGVGRIDHIENRLVGIKSREVYECPAAVTLITAHKELEDLTFVREVAHFKPIIEQKISETIYNGLWFSPLT
EALVAFLKSTQKFVNGTIRVKLFKGHAIVEGRKSPNSLYDENLATYTSSDTFDQDAAVGFIKLWGLPTKVSAEVNSKVTI
TTEV

Sequences:

>Translated_404_residues
MAKEKIVLAYSGGLDTSVAIQWLVESGYEVIACCLDVGEGKNLDFIKEKAITVGASESYTIDAKEEFAEDFALIALQAHA
YYEGKYPLISALSRPLIAKKLVEVARQEGASAIAHGCTGKGNDQVRFEVAIHALAPDLKVVSPVRDWKWSREEEINYAKE
HNIPVPIDLDNPFSIDQNLWGRSNECGVLENPWTTPPEAAYDLTVSLEDAPDTPDIVEITFDAGIPISLNGENMSLANLI
LTLNEIAGKHGVGRIDHIENRLVGIKSREVYECPAAVTLITAHKELEDLTFVREVAHFKPIIEQKISETIYNGLWFSPLT
EALVAFLKSTQKFVNGTIRVKLFKGHAIVEGRKSPNSLYDENLATYTSSDTFDQDAAVGFIKLWGLPTKVSAEVNSKVTI
TTEV
>Mature_403_residues
AKEKIVLAYSGGLDTSVAIQWLVESGYEVIACCLDVGEGKNLDFIKEKAITVGASESYTIDAKEEFAEDFALIALQAHAY
YEGKYPLISALSRPLIAKKLVEVARQEGASAIAHGCTGKGNDQVRFEVAIHALAPDLKVVSPVRDWKWSREEEINYAKEH
NIPVPIDLDNPFSIDQNLWGRSNECGVLENPWTTPPEAAYDLTVSLEDAPDTPDIVEITFDAGIPISLNGENMSLANLIL
TLNEIAGKHGVGRIDHIENRLVGIKSREVYECPAAVTLITAHKELEDLTFVREVAHFKPIIEQKISETIYNGLWFSPLTE
ALVAFLKSTQKFVNGTIRVKLFKGHAIVEGRKSPNSLYDENLATYTSSDTFDQDAAVGFIKLWGLPTKVSAEVNSKVTIT
TEV

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=408, Percent_Identity=41.9117647058824, Blast_Score=321, Evalue=6e-88,
Organism=Homo sapiens, GI16950633, Length=408, Percent_Identity=41.9117647058824, Blast_Score=321, Evalue=6e-88,
Organism=Escherichia coli, GI1789563, Length=367, Percent_Identity=26.9754768392371, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324514, Length=409, Percent_Identity=38.6308068459658, Blast_Score=292, Evalue=7e-80,
Organism=Drosophila melanogaster, GI21358151, Length=399, Percent_Identity=40.3508771929825, Blast_Score=283, Evalue=2e-76,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44478; Mature: 44347

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKIVLAYSGGLDTSVAIQWLVESGYEVIACCLDVGEGKNLDFIKEKAITVGASESYT
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHCEEEECCCCCEE
IDAKEEFAEDFALIALQAHAYYEGKYPLISALSRPLIAKKLVEVARQEGASAIAHGCTGK
ECCHHHHHHHHEEEEEEEEEEECCCCHHHHHHHCHHHHHHHHHHHHHCCCHHHHHCCCCC
GNDQVRFEVAIHALAPDLKVVSPVRDWKWSREEEINYAKEHNIPVPIDLDNPFSIDQNLW
CCCEEEEEEEEEECCCCCCEECCHHHCCCCCHHHCCHHHHCCCCEEEECCCCCCCCCCCC
GRSNECGVLENPWTTPPEAAYDLTVSLEDAPDTPDIVEITFDAGIPISLNGENMSLANLI
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCEEEECCCCCCHHHHH
LTLNEIAGKHGVGRIDHIENRLVGIKSREVYECPAAVTLITAHKELEDLTFVREVAHFKP
HHHHHHHCCCCCCCHHHHHHHEEECCCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHH
IIEQKISETIYNGLWFSPLTEALVAFLKSTQKFVNGTIRVKLFKGHAIVEGRKSPNSLYD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCEEEECCCCCCHHHH
ENLATYTSSDTFDQDAAVGFIKLWGLPTKVSAEVNSKVTITTEV
CCCCEECCCCCCCCCHHEEEEEEECCCCEEECCCCCEEEEEEEC
>Mature Secondary Structure 
AKEKIVLAYSGGLDTSVAIQWLVESGYEVIACCLDVGEGKNLDFIKEKAITVGASESYT
CCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHCEEEECCCCCEE
IDAKEEFAEDFALIALQAHAYYEGKYPLISALSRPLIAKKLVEVARQEGASAIAHGCTGK
ECCHHHHHHHHEEEEEEEEEEECCCCHHHHHHHCHHHHHHHHHHHHHCCCHHHHHCCCCC
GNDQVRFEVAIHALAPDLKVVSPVRDWKWSREEEINYAKEHNIPVPIDLDNPFSIDQNLW
CCCEEEEEEEEEECCCCCCEECCHHHCCCCCHHHCCHHHHCCCCEEEECCCCCCCCCCCC
GRSNECGVLENPWTTPPEAAYDLTVSLEDAPDTPDIVEITFDAGIPISLNGENMSLANLI
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCEEEECCCCCCHHHHH
LTLNEIAGKHGVGRIDHIENRLVGIKSREVYECPAAVTLITAHKELEDLTFVREVAHFKP
HHHHHHHCCCCCCCHHHHHHHEEECCCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHH
IIEQKISETIYNGLWFSPLTEALVAFLKSTQKFVNGTIRVKLFKGHAIVEGRKSPNSLYD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCEEEECCCCCCHHHH
ENLATYTSSDTFDQDAAVGFIKLWGLPTKVSAEVNSKVTITTEV
CCCCEECCCCCCCCCHHEEEEEEECCCCEEECCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]