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The map label for this gene is mscL

Identifier: 226224670

GI number: 226224670

Start: 2144763

End: 2145149

Strand: Direct

Name: mscL

Synonym: Lm4b_02085

Alternate gene names: 226224670

Gene position: 2144763-2145149 (Clockwise)

Preceding gene: 226224669

Following gene: 226224676

Centisome position: 73.64

GC content: 36.18

Gene sequence:

>387_bases
ATGAAAAAAATGTTAGTAGAATTTAGAGACTTCGCGTTAAAAGGGAATGTTCTAGACCTTGCTGTAGCGGTAGTTATCGG
GGCTGCTTTCGGTAAAATTGTTTCATCTTTAGTAGATAACATCATTATGCCACTTGTCGGTGTACTACTTGGTGGTCTTG
ATTTTACAGATTTAAGTTTCAAAGTTGGTAAATCCGTTATTCAGTACGGTGCTTTCATCCAGTCCATCGTTGACTTTGTC
ATCATCGCTTTTGCCATTTTTATTTTCGTCAAGGTACTTACTAGTTTTATTAAGAAAAAAGAGCAAACGGTGGAAGAAAC
GCCAGTACCTCCAACTGAGGAATACTTAAAAGAAATTCGGGATTTATTGAAAGAACAACAGAAATAA

Upstream 100 bases:

>100_bases
GCGCCAACGCTATACATAGGCAGTTTATTGCTAAAATAAGTTCAGTTGCTTTCACATCGCAGTTAGATTGTTTATACTGG
GAAGTGGGAGGTGAATTTTC

Downstream 100 bases:

>100_bases
CTGAAAAAATCCTTCTTTTGTGGAAGGATTTTTTATTTACATTCGCGCTTATAAATTATGTTTTCTAAAGCTAAAAAGTT
AGGCTATCTATCTCCCTTAA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MKKMLVEFRDFALKGNVLDLAVAVVIGAAFGKIVSSLVDNIIMPLVGVLLGGLDFTDLSFKVGKSVIQYGAFIQSIVDFV
IIAFAIFIFVKVLTSFIKKKEQTVEETPVPPTEEYLKEIRDLLKEQQK

Sequences:

>Translated_128_residues
MKKMLVEFRDFALKGNVLDLAVAVVIGAAFGKIVSSLVDNIIMPLVGVLLGGLDFTDLSFKVGKSVIQYGAFIQSIVDFV
IIAFAIFIFVKVLTSFIKKKEQTVEETPVPPTEEYLKEIRDLLKEQQK
>Mature_128_residues
MKKMLVEFRDFALKGNVLDLAVAVVIGAAFGKIVSSLVDNIIMPLVGVLLGGLDFTDLSFKVGKSVIQYGAFIQSIVDFV
IIAFAIFIFVKVLTSFIKKKEQTVEETPVPPTEEYLKEIRDLLKEQQK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=130, Percent_Identity=53.0769230769231, Blast_Score=129, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14225; Mature: 14225

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMLVEFRDFALKGNVLDLAVAVVIGAAFGKIVSSLVDNIIMPLVGVLLGGLDFTDLSF
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KVGKSVIQYGAFIQSIVDFVIIAFAIFIFVKVLTSFIKKKEQTVEETPVPPTEEYLKEIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DLLKEQQK
HHHHHHCC
>Mature Secondary Structure
MKKMLVEFRDFALKGNVLDLAVAVVIGAAFGKIVSSLVDNIIMPLVGVLLGGLDFTDLSF
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KVGKSVIQYGAFIQSIVDFVIIAFAIFIFVKVLTSFIKKKEQTVEETPVPPTEEYLKEIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DLLKEQQK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA