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The map label for this gene is exoA [H]

Identifier: 226224385

GI number: 226224385

Start: 1844294

End: 1845049

Strand: Direct

Name: exoA [H]

Synonym: Lm4b_01798

Alternate gene names: 226224385

Gene position: 1844294-1845049 (Clockwise)

Preceding gene: 226224382

Following gene: 226224391

Centisome position: 63.32

GC content: 37.96

Gene sequence:

>756_bases
GTGAAATTAATTTCTTGGAATGTAAACGGGCTTCGAGCAGCTGTAAAAAAAGGCTTTTTGGAGTATTTTGAAGAAGTAGA
TGCGGATATTTTTTGTTTGCAGGAAACTAAATTACAAGAAGGTCAAATTGAACTTGATTTGCCAGCATATAAAGATTACT
GGAATTATGCGGTGAAAAAAGGTTATTCTGGTACCGCGATTTTTACAAAAGTGGAGCCGTTATCAGTTCAATATGGTTTA
GGGGTTCCTGAACATGATACAGAAGGTCGTGTTATCACACTTGAATTTGAGGAATTTTTTATGGTGACGGTTTATACGCC
GAATTCGCAAGCTGAATTAAAACGATTAGATTACCGGATGACGTTTGAGGATGCGATTTTGGAATATGTAAAAAACTTGG
ATAAAACGAAGCCGGTTGTGCTTTGTGGCGATTTGAATGTTGCGCACGAAGAAATTGATTTAAAAAATCCGAAGACAAAT
CGTAAAAATGCTGGCTTCTCGGATGAGGAACGCGCGAAATTTTCTGCATTTTTAGATGCTGGATTTATTGATAGTTTCCG
TTATTTTTACCCAGATTTGACCGATGCTTATTCTTGGTGGTCTTACCGAATGAACGCGCGTGCTAGGAATACTGGTTGGC
GGATTGATTATTTTGTTGTATCGGAACGTTTGAAAGATAAGTTAGTGGATGCCAAAATTCATGCGGATGTGCTTGGTTCG
GATCATTGTCCTGTCGAGCTAGAACTTAATTTATAA

Upstream 100 bases:

>100_bases
ACTACATAATTCCCCATCTATCTCCCTCCCAATCGTTTAACTACATTATAGCATGTGACTTACGTTATAATAAGGCTATT
AGCGATGGGAGGTTTTTTAG

Downstream 100 bases:

>100_bases
CCTAAAAAAATCCTCACTCCAAAGAGTGAGGATTTTTGAATTATTTAGCTAATGCTTTTTTTGCTGAATCAGCAAGTGTG
TTGAATGATGCGATATCATT

Product: 3'-exo-deoxyribonuclease exoA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKLISWNVNGLRAAVKKGFLEYFEEVDADIFCLQETKLQEGQIELDLPAYKDYWNYAVKKGYSGTAIFTKVEPLSVQYGL
GVPEHDTEGRVITLEFEEFFMVTVYTPNSQAELKRLDYRMTFEDAILEYVKNLDKTKPVVLCGDLNVAHEEIDLKNPKTN
RKNAGFSDEERAKFSAFLDAGFIDSFRYFYPDLTDAYSWWSYRMNARARNTGWRIDYFVVSERLKDKLVDAKIHADVLGS
DHCPVELELNL

Sequences:

>Translated_251_residues
MKLISWNVNGLRAAVKKGFLEYFEEVDADIFCLQETKLQEGQIELDLPAYKDYWNYAVKKGYSGTAIFTKVEPLSVQYGL
GVPEHDTEGRVITLEFEEFFMVTVYTPNSQAELKRLDYRMTFEDAILEYVKNLDKTKPVVLCGDLNVAHEEIDLKNPKTN
RKNAGFSDEERAKFSAFLDAGFIDSFRYFYPDLTDAYSWWSYRMNARARNTGWRIDYFVVSERLKDKLVDAKIHADVLGS
DHCPVELELNL
>Mature_251_residues
MKLISWNVNGLRAAVKKGFLEYFEEVDADIFCLQETKLQEGQIELDLPAYKDYWNYAVKKGYSGTAIFTKVEPLSVQYGL
GVPEHDTEGRVITLEFEEFFMVTVYTPNSQAELKRLDYRMTFEDAILEYVKNLDKTKPVVLCGDLNVAHEEIDLKNPKTN
RKNAGFSDEERAKFSAFLDAGFIDSFRYFYPDLTDAYSWWSYRMNARARNTGWRIDYFVVSERLKDKLVDAKIHADVLGS
DHCPVELELNL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=257, Percent_Identity=50.1945525291829, Blast_Score=264, Evalue=6e-71,
Organism=Homo sapiens, GI18375503, Length=257, Percent_Identity=50.1945525291829, Blast_Score=264, Evalue=6e-71,
Organism=Homo sapiens, GI18375501, Length=257, Percent_Identity=50.1945525291829, Blast_Score=264, Evalue=6e-71,
Organism=Escherichia coli, GI1788046, Length=263, Percent_Identity=28.1368821292776, Blast_Score=107, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71989536, Length=257, Percent_Identity=44.7470817120623, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI71989539, Length=145, Percent_Identity=39.3103448275862, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI221330655, Length=254, Percent_Identity=50, Blast_Score=269, Evalue=1e-72,
Organism=Drosophila melanogaster, GI17136678, Length=254, Percent_Identity=50, Blast_Score=269, Evalue=2e-72,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29099; Mature: 29099

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGLRAAVKKGFLEYFEEVDADIFCLQETKLQEGQIELDLPAYKDYWNYAVKK
CEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHC
GYSGTAIFTKVEPLSVQYGLGVPEHDTEGRVITLEFEEFFMVTVYTPNSQAELKRLDYRM
CCCCCEEEEEECCEEEEECCCCCCCCCCCCEEEEEEHHEEEEEEECCCCHHHHHHHHEEE
TFEDAILEYVKNLDKTKPVVLCGDLNVAHEEIDLKNPKTNRKNAGFSDEERAKFSAFLDA
CHHHHHHHHHHCCCCCCCEEEECCCCCCHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHC
GFIDSFRYFYPDLTDAYSWWSYRMNARARNTGWRIDYFVVSERLKDKLVDAKIHADVLGS
CHHHHHHHHCCCHHHHHHHHHEEECCEECCCCCEEEEEEHHHHHHHHHHHHHEEHEECCC
DHCPVELELNL
CCCCEEEEECC
>Mature Secondary Structure
MKLISWNVNGLRAAVKKGFLEYFEEVDADIFCLQETKLQEGQIELDLPAYKDYWNYAVKK
CEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHC
GYSGTAIFTKVEPLSVQYGLGVPEHDTEGRVITLEFEEFFMVTVYTPNSQAELKRLDYRM
CCCCCEEEEEECCEEEEECCCCCCCCCCCCEEEEEEHHEEEEEEECCCCHHHHHHHHEEE
TFEDAILEYVKNLDKTKPVVLCGDLNVAHEEIDLKNPKTNRKNAGFSDEERAKFSAFLDA
CHHHHHHHHHHCCCCCCCEEEECCCCCCHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHC
GFIDSFRYFYPDLTDAYSWWSYRMNARARNTGWRIDYFVVSERLKDKLVDAKIHADVLGS
CHHHHHHHHCCCHHHHHHHHHEEECCEECCCCCEEEEEEHHHHHHHHHHHHHEEHEECCC
DHCPVELELNL
CCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]