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The map label for this gene is map [H]

Identifier: 226224312

GI number: 226224312

Start: 1757056

End: 1757814

Strand: Reverse

Name: map [H]

Synonym: Lm4b_01723

Alternate gene names: 226224312

Gene position: 1757814-1757056 (Counterclockwise)

Preceding gene: 226224317

Following gene: 226224311

Centisome position: 60.35

GC content: 39.92

Gene sequence:

>759_bases
ATGATCACACTCAAATCAAGACGCGAAATCGATGAGATGAAAGCAGCAGGGAAAATCCTTGCCGATACGCATAAAGAACT
TAAAAAAATTATTAAACCAGGTATTACTAGCTGGGATTTAGAAGTATTTACAGAAGAATTTTTACGTAAAAATGGCGCGA
CTCCAGAGCAAAAAGGCTTTGAAGGTTATGAATATGCTATTTGTGCAAGTATTAATGACGAAATTTGTCATGGCTTCCCG
CGCAAACAAAAGCTTAACCAAGGCGATATTATTACAGTAGATATGGTCGTGAATTATCGCGGTGCACTTGCTGATTCTGC
TTGGACTTATGCCGTAGGTGAAGTGCCAGATGATGTGAAACATTTAATGGATGTAACGCATAAAGCACTTTATTTAGGGA
TTGAACAAGCACAAGTTGGCGCACGAGTTGGCGATATTGGTCATGCTATTCAAACATATGTAGAATCAGAAAATCTTGCT
GTTGTACGTGAGTTTATTGGTCACGGCGTTGGACCAACTTTACATGAAAAACCAGATATCCCACATTACGGTACAGCTGG
CAAAGGACCTCGCTTGAAAGAAGGCATGGTTATTACAGTGGAACCAATGGTCAATATGGGTGCTTGGAAAGCCAAAATGG
ACGATAATGGCTGGACTGCTAGAACTGTTGATGGCTCTTTGTCTGCGCAATATGAACATACTTTTGCAATCACAAAAGAT
GGTCCAGAAATTTTAACGTATCAAGGCGAAAACGATTAA

Upstream 100 bases:

>100_bases
GTCTTCAGAAAAACTGCAACCTTTTTTCCAGATTACTTTATTTCTAAGGGGTTTCGAGCTATAATAGTAAATAGTGGAAA
TTTTAGGAGGGAATGAAAAG

Downstream 100 bases:

>100_bases
TTAAACTATTTAGCCAGATGCATTTAGAACAGAATGCAAGTAAAAGCACAAGTTTCACACAGCTGCTTTTAGTTTGCCGT
TTTTTTCTAGGCATCTGGCT

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MITLKSRREIDEMKAAGKILADTHKELKKIIKPGITSWDLEVFTEEFLRKNGATPEQKGFEGYEYAICASINDEICHGFP
RKQKLNQGDIITVDMVVNYRGALADSAWTYAVGEVPDDVKHLMDVTHKALYLGIEQAQVGARVGDIGHAIQTYVESENLA
VVREFIGHGVGPTLHEKPDIPHYGTAGKGPRLKEGMVITVEPMVNMGAWKAKMDDNGWTARTVDGSLSAQYEHTFAITKD
GPEILTYQGEND

Sequences:

>Translated_252_residues
MITLKSRREIDEMKAAGKILADTHKELKKIIKPGITSWDLEVFTEEFLRKNGATPEQKGFEGYEYAICASINDEICHGFP
RKQKLNQGDIITVDMVVNYRGALADSAWTYAVGEVPDDVKHLMDVTHKALYLGIEQAQVGARVGDIGHAIQTYVESENLA
VVREFIGHGVGPTLHEKPDIPHYGTAGKGPRLKEGMVITVEPMVNMGAWKAKMDDNGWTARTVDGSLSAQYEHTFAITKD
GPEILTYQGEND
>Mature_252_residues
MITLKSRREIDEMKAAGKILADTHKELKKIIKPGITSWDLEVFTEEFLRKNGATPEQKGFEGYEYAICASINDEICHGFP
RKQKLNQGDIITVDMVVNYRGALADSAWTYAVGEVPDDVKHLMDVTHKALYLGIEQAQVGARVGDIGHAIQTYVESENLA
VVREFIGHGVGPTLHEKPDIPHYGTAGKGPRLKEGMVITVEPMVNMGAWKAKMDDNGWTARTVDGSLSAQYEHTFAITKD
GPEILTYQGEND

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=245, Percent_Identity=33.0612244897959, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI40385867, Length=245, Percent_Identity=30.6122448979592, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1786364, Length=252, Percent_Identity=36.5079365079365, Blast_Score=185, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71996291, Length=252, Percent_Identity=32.1428571428571, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6323273, Length=217, Percent_Identity=37.7880184331797, Blast_Score=162, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21355531, Length=244, Percent_Identity=35.655737704918, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24583427, Length=243, Percent_Identity=35.3909465020576, Blast_Score=164, Evalue=6e-41,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 27902; Mature: 27902

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00680 MAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLKSRREIDEMKAAGKILADTHKELKKIIKPGITSWDLEVFTEEFLRKNGATPEQKGF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
EGYEYAICASINDEICHGFPRKQKLNQGDIITVDMVVNYRGALADSAWTYAVGEVPDDVK
CCCEEEEEECCCHHHHCCCCCHHCCCCCCEEEEEEEEECCCCCCCCCCEEEECCCCHHHH
HLMDVTHKALYLGIEQAQVGARVGDIGHAIQTYVESENLAVVREFIGHGVGPTLHEKPDI
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCC
PHYGTAGKGPRLKEGMVITVEPMVNMGAWKAKMDDNGWTARTVDGSLSAQYEHTFAITKD
CCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCEEEEECCCCCCCEEEEEEEECC
GPEILTYQGEND
CCEEEEECCCCC
>Mature Secondary Structure
MITLKSRREIDEMKAAGKILADTHKELKKIIKPGITSWDLEVFTEEFLRKNGATPEQKGF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
EGYEYAICASINDEICHGFPRKQKLNQGDIITVDMVVNYRGALADSAWTYAVGEVPDDVK
CCCEEEEEECCCHHHHCCCCCHHCCCCCCEEEEEEEEECCCCCCCCCCEEEECCCCHHHH
HLMDVTHKALYLGIEQAQVGARVGDIGHAIQTYVESENLAVVREFIGHGVGPTLHEKPDI
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCC
PHYGTAGKGPRLKEGMVITVEPMVNMGAWKAKMDDNGWTARTVDGSLSAQYEHTFAITKD
CCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCEEEEECCCCCCCEEEEEEEECC
GPEILTYQGEND
CCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192928; 11058132 [H]