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The map label for this gene is dut [H]

Identifier: 226224294

GI number: 226224294

Start: 1742559

End: 1743020

Strand: Reverse

Name: dut [H]

Synonym: Lm4b_01705

Alternate gene names: 226224294

Gene position: 1743020-1742559 (Counterclockwise)

Preceding gene: 226224295

Following gene: 226224292

Centisome position: 59.84

GC content: 37.88

Gene sequence:

>462_bases
TTGAAAGTAAGAGGATTTGAAGTAGTAAATGAAGCAAGCAGAAAATTTCCGGAACAAACAATATCTTTACCAATACGCGG
GGACAAAGGTTCGGCCGGCTATGATTTCTTCTCTAATGAAACAGTAACCATCTTACCAGGAGAAAAACACATTTTTTGGA
CAGATATTAAAAGTTATATGCAGGAAGAGGAGGTTTTGAATATCTATGTCCGTTCTTCCATTGGTATAAAGAAAGGTTTA
CTTCTGTGTAACGGTACAGGAATTATTGATTCTTCTTATTATAGTAATCCAGGTAATGATGGCAATATTGGGATTGCCAT
TAAAAACTTCTCCAATGAAGCAGTAGCTATTGAAGCGGGGGAACGTGTTGCTCAAGGCGTATTTCAAAAATACTTAGTTG
CAGATACAGATATTGTCGCAAACGAATCCCGTGTTGGTGGGGTAGGAAGTACAGGCAGATAA

Upstream 100 bases:

>100_bases
CAGAGTACGCGGCAGATTATGTCCGTCAGCAAATCGAGCGCCACGGCAGTTTTTTACACCAAGTAGAACGAAAAGACCCA
AACAAATAGGAGTGAACGAA

Downstream 100 bases:

>100_bases
AAAAAGACCCGGGATTCCGGGTCTTTTTTTAAATACTAACAGGTGCTAGTTTTTTGTATAATTTTTCTGTGGAGAAAATC
CAACCAGTGTAAGATGAAAC

Product: deoxyuridine triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MKVRGFEVVNEASRKFPEQTISLPIRGDKGSAGYDFFSNETVTILPGEKHIFWTDIKSYMQEEEVLNIYVRSSIGIKKGL
LLCNGTGIIDSSYYSNPGNDGNIGIAIKNFSNEAVAIEAGERVAQGVFQKYLVADTDIVANESRVGGVGSTGR

Sequences:

>Translated_153_residues
MKVRGFEVVNEASRKFPEQTISLPIRGDKGSAGYDFFSNETVTILPGEKHIFWTDIKSYMQEEEVLNIYVRSSIGIKKGL
LLCNGTGIIDSSYYSNPGNDGNIGIAIKNFSNEAVAIEAGERVAQGVFQKYLVADTDIVANESRVGGVGSTGR
>Mature_153_residues
MKVRGFEVVNEASRKFPEQTISLPIRGDKGSAGYDFFSNETVTILPGEKHIFWTDIKSYMQEEEVLNIYVRSSIGIKKGL
LLCNGTGIIDSSYYSNPGNDGNIGIAIKNFSNEAVAIEAGERVAQGVFQKYLVADTDIVANESRVGGVGSTGR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Escherichia coli, GI1790071, Length=147, Percent_Identity=29.9319727891156, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 16645; Mature: 16645

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRGFEVVNEASRKFPEQTISLPIRGDKGSAGYDFFSNETVTILPGEKHIFWTDIKSYM
CCCCCHHHHHHHHHCCCCHHEEEEEECCCCCCCCEEECCCEEEEECCCCEEEHHHHHHHH
QEEEVLNIYVRSSIGIKKGLLLCNGTGIIDSSYYSNPGNDGNIGIAIKNFSNEAVAIEAG
HHHHHEEEEEECCCCCCCCEEEECCCCEECCCCCCCCCCCCEEEEEEECCCCCEEEEECC
ERVAQGVFQKYLVADTDIVANESRVGGVGSTGR
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKVRGFEVVNEASRKFPEQTISLPIRGDKGSAGYDFFSNETVTILPGEKHIFWTDIKSYM
CCCCCHHHHHHHHHCCCCHHEEEEEECCCCCCCCEEECCCEEEEECCCCEEEHHHHHHHH
QEEEVLNIYVRSSIGIKKGLLLCNGTGIIDSSYYSNPGNDGNIGIAIKNFSNEAVAIEAG
HHHHHEEEEEECCCCCCCCEEEECCCCEECCCCCCCCCCCCEEEEEEECCCCCEEEEECC
ERVAQGVFQKYLVADTDIVANESRVGGVGSTGR
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]