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The map label for this gene is tsf

Identifier: 226224257

GI number: 226224257

Start: 1689356

End: 1690240

Strand: Reverse

Name: tsf

Synonym: Lm4b_01667

Alternate gene names: 226224257

Gene position: 1690240-1689356 (Counterclockwise)

Preceding gene: 226224258

Following gene: 226224256

Centisome position: 58.03

GC content: 37.29

Gene sequence:

>885_bases
ATGGCTAATATTACAGCTCAAATGGTAAAAGAATTACGCGAAAAAACTGGTGCTGGTATGATGGATTGTAAAAAAGCACT
TGTAGAAACAGAAGGAGATATGGAAAAAGCAATTGACTATCTTCGTGAAAAAGGAATCGCTAAAGCTGCGAAAAAATCTG
ATCGTGTTGCTTCTGAAGGTATGACTCATGTAATCAGCAATGAAAAACATGCCGTAGTACTTGAAGTAAATGCTGAAACA
GATTTCGTTGCTAAAAACGATAACTTCCAACAATTAGTTGACGCTTTAGCTAAACAAATTCTTGCAGTTCGTCCAGATAG
CTTAGAAGATGCGCTTAAAACAGAAATGCCTAATGGTCAAACTGTTCAAGACTACATCACTGAAGCAATTACAAAAATCG
GTGAAAACATTTCCCTTCGTCGTTTTGAAGTAAAAGAAAAAGCAGACAACTCTGCTTTCGGTGAATACATCCACATGAAC
GGACGTATTGGTGTTCTTACACTTCTTGAAGGAACTACTGATACTACTGTTGCAAAAGACGTTGCAATGCACATCGCTGC
AATCAACCCTAAATACATTTCTCGTGAAGACGTTTCTACTGAAGAAGTGGAACACGAAAAAGAAGTATTAACTCAACAAG
CGTTAAACGAAGGCAAACCAGCTAATATCGTTGAAAAAATGGTAGAAGGCCGTTTGAAAAAATATCTAAGCGAAATTTCC
TTAGAAGACCAACCTTTCGTTAAAAACCCAGACATCACTGTAGGTGACTACGTGAAACAAAGCGGTGGTAAAGTTGTATC
ATTCGTACGTTTCGAAGTAGGCGAAGGAATCGAGAAAAAAGAAGATAACTTTGTTGAAGAAGTAATGAGCCAAGTGAAAA
AATAA

Upstream 100 bases:

>100_bases
GAAGAAACTACTGAAGCATAAGATTTTATTTGAAACTTTCAAGGTGATAAGGCACTATTCTTATCACCTTTTTTAAAGAG
AAAAATTGGAGGGAAAATAA

Downstream 100 bases:

>100_bases
ATCCACTTTAAATCGATTTTATATGATATCAAAACCCCGTATTTTCTTAGGAAATACGGGGTTTTTATTTTTTTGATTTC
ATTTGAATATCATACAATAT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MANITAQMVKELREKTGAGMMDCKKALVETEGDMEKAIDYLREKGIAKAAKKSDRVASEGMTHVISNEKHAVVLEVNAET
DFVAKNDNFQQLVDALAKQILAVRPDSLEDALKTEMPNGQTVQDYITEAITKIGENISLRRFEVKEKADNSAFGEYIHMN
GRIGVLTLLEGTTDTTVAKDVAMHIAAINPKYISREDVSTEEVEHEKEVLTQQALNEGKPANIVEKMVEGRLKKYLSEIS
LEDQPFVKNPDITVGDYVKQSGGKVVSFVRFEVGEGIEKKEDNFVEEVMSQVKK

Sequences:

>Translated_294_residues
MANITAQMVKELREKTGAGMMDCKKALVETEGDMEKAIDYLREKGIAKAAKKSDRVASEGMTHVISNEKHAVVLEVNAET
DFVAKNDNFQQLVDALAKQILAVRPDSLEDALKTEMPNGQTVQDYITEAITKIGENISLRRFEVKEKADNSAFGEYIHMN
GRIGVLTLLEGTTDTTVAKDVAMHIAAINPKYISREDVSTEEVEHEKEVLTQQALNEGKPANIVEKMVEGRLKKYLSEIS
LEDQPFVKNPDITVGDYVKQSGGKVVSFVRFEVGEGIEKKEDNFVEEVMSQVKK
>Mature_293_residues
ANITAQMVKELREKTGAGMMDCKKALVETEGDMEKAIDYLREKGIAKAAKKSDRVASEGMTHVISNEKHAVVLEVNAETD
FVAKNDNFQQLVDALAKQILAVRPDSLEDALKTEMPNGQTVQDYITEAITKIGENISLRRFEVKEKADNSAFGEYIHMNG
RIGVLTLLEGTTDTTVAKDVAMHIAAINPKYISREDVSTEEVEHEKEVLTQQALNEGKPANIVEKMVEGRLKKYLSEISL
EDQPFVKNPDITVGDYVKQSGGKVVSFVRFEVGEGIEKKEDNFVEEVMSQVKK

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=299, Percent_Identity=28.0936454849498, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI291084500, Length=320, Percent_Identity=27.1875, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI291084502, Length=95, Percent_Identity=38.9473684210526, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI291084498, Length=95, Percent_Identity=38.9473684210526, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1786366, Length=294, Percent_Identity=45.9183673469388, Blast_Score=236, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17561440, Length=300, Percent_Identity=27, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI19921466, Length=303, Percent_Identity=26.7326732673267, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 32697; Mature: 32565

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANITAQMVKELREKTGAGMMDCKKALVETEGDMEKAIDYLREKGIAKAAKKSDRVASEG
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
MTHVISNEKHAVVLEVNAETDFVAKNDNFQQLVDALAKQILAVRPDSLEDALKTEMPNGQ
HHHHHCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
TVQDYITEAITKIGENISLRRFEVKEKADNSAFGEYIHMNGRIGVLTLLEGTTDTTVAKD
CHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCHHCCEEEECCCEEEEEEECCCCCHHHHHH
VAMHIAAINPKYISREDVSTEEVEHEKEVLTQQALNEGKPANIVEKMVEGRLKKYLSEIS
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
LEDQPFVKNPDITVGDYVKQSGGKVVSFVRFEVGEGIEKKEDNFVEEVMSQVKK
CCCCCCCCCCCCCHHHHHHHCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANITAQMVKELREKTGAGMMDCKKALVETEGDMEKAIDYLREKGIAKAAKKSDRVASEG
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
MTHVISNEKHAVVLEVNAETDFVAKNDNFQQLVDALAKQILAVRPDSLEDALKTEMPNGQ
HHHHHCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
TVQDYITEAITKIGENISLRRFEVKEKADNSAFGEYIHMNGRIGVLTLLEGTTDTTVAKD
CHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCHHCCEEEECCCEEEEEEECCCCCHHHHHH
VAMHIAAINPKYISREDVSTEEVEHEKEVLTQQALNEGKPANIVEKMVEGRLKKYLSEIS
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
LEDQPFVKNPDITVGDYVKQSGGKVVSFVRFEVGEGIEKKEDNFVEEVMSQVKK
CCCCCCCCCCCCCHHHHHHHCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]