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The map label for this gene is sbcD [H]

Identifier: 226224247

GI number: 226224247

Start: 1678752

End: 1679876

Strand: Reverse

Name: sbcD [H]

Synonym: Lm4b_01657

Alternate gene names: 226224247

Gene position: 1679876-1678752 (Counterclockwise)

Preceding gene: 226224249

Following gene: 226224246

Centisome position: 57.67

GC content: 40.27

Gene sequence:

>1125_bases
ATGAAATTTTTACATACAGCTGATTTGCATTTAGGAAAAATTGTATCTGGCGTATCGATGCTAGCAGAGCAAGAATATAT
TTTGGCACAAATAACGAAGATTGCCGAGGAAGAACAAGTCGACGCACTTATTTTAGCAGGAGATTTATATGACCGCGCCG
TGCCGCCTGCGGATGCGGTGAAAGTGCTAAATGATATTTTAGTGAAATGGAATGTAGAACTAGGTATTCCGATATTTGCC
ATTAGCGGGAATCATGACAGTGCGGAGCGGCTTGCTTTTGGAAGTCAGTGGTATGAAAGTAGCAAGTTGTATATGAAAGG
AAAATGTACCTCGCAGTTTGAAGCGATTTCTTTTATGGATGCCGAAGTGTGGCTTGTACCATATCACGAACCAGCAATTA
TTCGGGAAGCTTTTGCAGATAATTCGATTCGCAGTTTTGAGGACGCTATGCAAGCCGTGACGAAACAAATTCGGTCCAAA
TGGGATACAAGCAAGGCGCAGATTTTGGTTGGACATGCGTTCGTTTCTGGCGGTATTCCGAGTGATTCCGAACGACAACT
AGCAATTGGAAATGTGGACCGTGTTTCTACGGATTGTTTTGATGGTTTTACATATACTGCACTCGGGCACTTGCACCATC
CGCATGCGATTCGGCACCCGTCTATCTTTTATAGTGGATCTCCTTTAAAGTATTCATTTTCAGAAGCAAATGATCACAAA
AGCGTGCGGATTGTGGAACTGGAAGGAAAAGAACTGGTTAGCGTGACGGAGCGCTTTCTAACACCAAAACATGATATGCG
CATTATTTCTGGAACACTAGCGGAACTGACAGAAAACTTAGTAGAAAATCCTGATGACTTTTTTCAAGTGAATTTAATGG
ATGAAGGTGCTTTAATTGATCCGATGGGGAAATTACGCCAATTTTATCCGAATATTTTACATTTAGAACGTAAAAAACAA
ACATTAAAAGAAACGCAAGATAACTTTGAAGAAATCATGAAAAAAGATGATTTAGAGCTATTTGGTCAATTTTTTGAACA
TGTTAATGGGACAGAATTAACGGAAACACAAAAACAAAAGTTAGCGGATGTTTTTGAACAAGCGAGGAAGGAGGATGCGG
AATGA

Upstream 100 bases:

>100_bases
TATTGCTAGTTATTTTTTTAAACTATATCTAGTATACTTAAAATTGACGAGTTTTGCATTAAAAATAAATAATCTCTGAG
AAAAAGAAATGAGGTAACCG

Downstream 100 bases:

>100_bases
GACCGATTAAATTAACGATGCAAGCTTTTGGAGCCTATGCCAAAAAAGAAGTCATTGATTTTGAAAAGCTTGGAACGGAG
CAAATTTTTGTGATTTCTGG

Product: exonucleases SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKFLHTADLHLGKIVSGVSMLAEQEYILAQITKIAEEEQVDALILAGDLYDRAVPPADAVKVLNDILVKWNVELGIPIFA
ISGNHDSAERLAFGSQWYESSKLYMKGKCTSQFEAISFMDAEVWLVPYHEPAIIREAFADNSIRSFEDAMQAVTKQIRSK
WDTSKAQILVGHAFVSGGIPSDSERQLAIGNVDRVSTDCFDGFTYTALGHLHHPHAIRHPSIFYSGSPLKYSFSEANDHK
SVRIVELEGKELVSVTERFLTPKHDMRIISGTLAELTENLVENPDDFFQVNLMDEGALIDPMGKLRQFYPNILHLERKKQ
TLKETQDNFEEIMKKDDLELFGQFFEHVNGTELTETQKQKLADVFEQARKEDAE

Sequences:

>Translated_374_residues
MKFLHTADLHLGKIVSGVSMLAEQEYILAQITKIAEEEQVDALILAGDLYDRAVPPADAVKVLNDILVKWNVELGIPIFA
ISGNHDSAERLAFGSQWYESSKLYMKGKCTSQFEAISFMDAEVWLVPYHEPAIIREAFADNSIRSFEDAMQAVTKQIRSK
WDTSKAQILVGHAFVSGGIPSDSERQLAIGNVDRVSTDCFDGFTYTALGHLHHPHAIRHPSIFYSGSPLKYSFSEANDHK
SVRIVELEGKELVSVTERFLTPKHDMRIISGTLAELTENLVENPDDFFQVNLMDEGALIDPMGKLRQFYPNILHLERKKQ
TLKETQDNFEEIMKKDDLELFGQFFEHVNGTELTETQKQKLADVFEQARKEDAE
>Mature_374_residues
MKFLHTADLHLGKIVSGVSMLAEQEYILAQITKIAEEEQVDALILAGDLYDRAVPPADAVKVLNDILVKWNVELGIPIFA
ISGNHDSAERLAFGSQWYESSKLYMKGKCTSQFEAISFMDAEVWLVPYHEPAIIREAFADNSIRSFEDAMQAVTKQIRSK
WDTSKAQILVGHAFVSGGIPSDSERQLAIGNVDRVSTDCFDGFTYTALGHLHHPHAIRHPSIFYSGSPLKYSFSEANDHK
SVRIVELEGKELVSVTERFLTPKHDMRIISGTLAELTENLVENPDDFFQVNLMDEGALIDPMGKLRQFYPNILHLERKKQ
TLKETQDNFEEIMKKDDLELFGQFFEHVNGTELTETQKQKLADVFEQARKEDAE

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=388, Percent_Identity=25.7731958762887, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 42323; Mature: 42323

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLHTADLHLGKIVSGVSMLAEQEYILAQITKIAEEEQVDALILAGDLYDRAVPPADAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHH
KVLNDILVKWNVELGIPIFAISGNHDSAERLAFGSQWYESSKLYMKGKCTSQFEAISFMD
HHHHHHHHEEEEEECCEEEEEECCCCHHHHHHHCHHHHCCCCEEEECCCCCHHHHHEEEC
AEVWLVPYHEPAIIREAFADNSIRSFEDAMQAVTKQIRSKWDTSKAQILVGHAFVSGGIP
CEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEHEECCCCC
SDSERQLAIGNVDRVSTDCFDGFTYTALGHLHHPHAIRHPSIFYSGSPLKYSFSEANDHK
CCCCCEEEECCCHHCCHHHHCCCHHHHHHHCCCCCCCCCCCEEECCCCCEEECCCCCCCC
SVRIVELEGKELVSVTERFLTPKHDMRIISGTLAELTENLVENPDDFFQVNLMDEGALID
CEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEC
PMGKLRQFYPNILHLERKKQTLKETQDNFEEIMKKDDLELFGQFFEHVNGTELTETQKQK
CHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHH
LADVFEQARKEDAE
HHHHHHHHHHCCCC
>Mature Secondary Structure
MKFLHTADLHLGKIVSGVSMLAEQEYILAQITKIAEEEQVDALILAGDLYDRAVPPADAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHH
KVLNDILVKWNVELGIPIFAISGNHDSAERLAFGSQWYESSKLYMKGKCTSQFEAISFMD
HHHHHHHHEEEEEECCEEEEEECCCCHHHHHHHCHHHHCCCCEEEECCCCCHHHHHEEEC
AEVWLVPYHEPAIIREAFADNSIRSFEDAMQAVTKQIRSKWDTSKAQILVGHAFVSGGIP
CEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEHEECCCCC
SDSERQLAIGNVDRVSTDCFDGFTYTALGHLHHPHAIRHPSIFYSGSPLKYSFSEANDHK
CCCCCEEEECCCHHCCHHHHCCCHHHHHHHCCCCCCCCCCCEEECCCCCEEECCCCCCCC
SVRIVELEGKELVSVTERFLTPKHDMRIISGTLAELTENLVENPDDFFQVNLMDEGALID
CEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEC
PMGKLRQFYPNILHLERKKQTLKETQDNFEEIMKKDDLELFGQFFEHVNGTELTETQKQK
CHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHH
LADVFEQARKEDAE
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA