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The map label for this gene is daaA

Identifier: 226224220

GI number: 226224220

Start: 1645299

End: 1646168

Strand: Reverse

Name: daaA

Synonym: Lm4b_01630

Alternate gene names: 226224220

Gene position: 1646168-1645299 (Counterclockwise)

Preceding gene: 226224221

Following gene: 226224219

Centisome position: 56.52

GC content: 37.13

Gene sequence:

>870_bases
ATGAAAGTATTAGTAAATAACCATTTAGTGGAAAGAGAAGATGCCACAGTTGACATTGAAGACCGCGGATATCAGTTTGG
TGATGGTGTATATGAAGTAGTTCGTCTATATAATGGAAAATTCTTCACTTATAATGAACACATTGATCGTTTATATGCGA
GTGCAGCAAAAATTGACTTAGTTATTCCTTATTCGAAAGAAGAGTTACGAGCGTTACTTGAAAAATTAGTTGCTGAAAAT
AATATTAATACAGGAAATGTCTATTTACAAGTGACTCGAGGTGTTCAAAACCCGCGTAATCACGTTATGCCAGATGATTT
CCCGCTGGAAGGCGTTTTAACAGCAGCAGCTCGTGAAGTACCAAGAAATGAACAACAATTTGTGCAAGGTGGACCAGTAA
TTACAGAAGAAGATGTTCGTTGGTTACGCTGTGACATCAAGAGTTTGAATTTACTTGGAAACATTTTAGCAAAAAACAAA
GCACATCAACAAAATGCGTTAGAAGCTGTTTTACACCGCGGAGAGCAAGTAACTGAGTGTTCAGCTTCCAATATTTCTAT
TATTAAAGACGGTGTGTTATGGACTCACGCGGCAGATAATTTAATCTTAAATGGTATTACTCGTCAAGTAATTATTGCTG
TTGCGAAAAAGAATGGTATTCCGGTCAAAGAAGCTGATTTTACTTTAACAGATCTTCGTGAAGCCGATGAAGTATTCATT
TCAAGCACAACAATCGAAATTACCCCAGTTACCCATATCGACGGAGTGCAAGTAGCTGACGGAAAACGCGGACCTATTAC
CGCAAAACTTCATCAATATTTTGTAGAAGAAATCGTTCAAGCATGTGGCGAATTAGAATTTGCAAAATAA

Upstream 100 bases:

>100_bases
AAAAAATTAACTGCTGCAAAGCTTAGTTTTGCGGCAGTTTCTTTGTTTCATTAAGTTTTTAGATAGTTCCAAAAATTAAC
TATGCAAGGAGAGACTTGAG

Downstream 100 bases:

>100_bases
TAAGTAGAAACCTTCCTTTTTTAGGAAGGTTTTTTTGCGTCTTGATACCTATGTAAAAATTTATATAACTTTTTTATCCG
TTGAAATTACGCCATTGGCT

Product: D-Amino Acid Aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKVLVNNHLVEREDATVDIEDRGYQFGDGVYEVVRLYNGKFFTYNEHIDRLYASAAKIDLVIPYSKEELRALLEKLVAEN
NINTGNVYLQVTRGVQNPRNHVMPDDFPLEGVLTAAAREVPRNEQQFVQGGPVITEEDVRWLRCDIKSLNLLGNILAKNK
AHQQNALEAVLHRGEQVTECSASNISIIKDGVLWTHAADNLILNGITRQVIIAVAKKNGIPVKEADFTLTDLREADEVFI
SSTTIEITPVTHIDGVQVADGKRGPITAKLHQYFVEEIVQACGELEFAK

Sequences:

>Translated_289_residues
MKVLVNNHLVEREDATVDIEDRGYQFGDGVYEVVRLYNGKFFTYNEHIDRLYASAAKIDLVIPYSKEELRALLEKLVAEN
NINTGNVYLQVTRGVQNPRNHVMPDDFPLEGVLTAAAREVPRNEQQFVQGGPVITEEDVRWLRCDIKSLNLLGNILAKNK
AHQQNALEAVLHRGEQVTECSASNISIIKDGVLWTHAADNLILNGITRQVIIAVAKKNGIPVKEADFTLTDLREADEVFI
SSTTIEITPVTHIDGVQVADGKRGPITAKLHQYFVEEIVQACGELEFAK
>Mature_289_residues
MKVLVNNHLVEREDATVDIEDRGYQFGDGVYEVVRLYNGKFFTYNEHIDRLYASAAKIDLVIPYSKEELRALLEKLVAEN
NINTGNVYLQVTRGVQNPRNHVMPDDFPLEGVLTAAAREVPRNEQQFVQGGPVITEEDVRWLRCDIKSLNLLGNILAKNK
AHQQNALEAVLHRGEQVTECSASNISIIKDGVLWTHAADNLILNGITRQVIIAVAKKNGIPVKEADFTLTDLREADEVFI
SSTTIEITPVTHIDGVQVADGKRGPITAKLHQYFVEEIVQACGELEFAK

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=293, Percent_Identity=30.3754266211604, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787338, Length=276, Percent_Identity=26.8115942028986, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 32300; Mature: 32300

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVNNHLVEREDATVDIEDRGYQFGDGVYEVVRLYNGKFFTYNEHIDRLYASAAKIDL
CEEEECCCEECCCCCEEEECCCCEECCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHEEEE
VIPYSKEELRALLEKLVAENNINTGNVYLQVTRGVQNPRNHVMPDDFPLEGVLTAAAREV
EECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHC
PRNEQQFVQGGPVITEEDVRWLRCDIKSLNLLGNILAKNKAHQQNALEAVLHRGEQVTEC
CCCHHHHHCCCCEEECCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHC
SASNISIIKDGVLWTHAADNLILNGITRQVIIAVAKKNGIPVKEADFTLTDLREADEVFI
CCCCCEEEECCEEEEECCCCHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHEEE
SSTTIEITPVTHIDGVQVADGKRGPITAKLHQYFVEEIVQACGELEFAK
ECCEEEEEEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVLVNNHLVEREDATVDIEDRGYQFGDGVYEVVRLYNGKFFTYNEHIDRLYASAAKIDL
CEEEECCCEECCCCCEEEECCCCEECCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHEEEE
VIPYSKEELRALLEKLVAENNINTGNVYLQVTRGVQNPRNHVMPDDFPLEGVLTAAAREV
EECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHC
PRNEQQFVQGGPVITEEDVRWLRCDIKSLNLLGNILAKNKAHQQNALEAVLHRGEQVTEC
CCCHHHHHCCCCEEECCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHC
SASNISIIKDGVLWTHAADNLILNGITRQVIIAVAKKNGIPVKEADFTLTDLREADEVFI
CCCCCEEEECCEEEEECCCCHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHEEE
SSTTIEITPVTHIDGVQVADGKRGPITAKLHQYFVEEIVQACGELEFAK
ECCEEEEEEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]