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The map label for this gene is thrS

Identifier: 226224160

GI number: 226224160

Start: 1578926

End: 1580848

Strand: Reverse

Name: thrS

Synonym: Lm4b_01570

Alternate gene names: 226224160

Gene position: 1580848-1578926 (Counterclockwise)

Preceding gene: 226224161

Following gene: 226224159

Centisome position: 54.27

GC content: 39.42

Gene sequence:

>1923_bases
ATGAAAATCACATTTCCAGACGGTGCAGTAAAAGAATTCGAGCCAGGCGTTTCAACAGCAGATATTGCTGCTTCTATCAG
CCCAGGGCTAAAAAAGAAAGCACTCGCAGGTAAATTAAACGGGGAGTTACTTGATTTAGTAACACCAATCCACGAAGACG
GAGCAATCGAAATCGTAACACCAGACCATGAAGATGCGCTTGGTATTTTGCGTCACAGTACAGCTCATTTAATGGCTCAA
GCCTTGAAACGCCTTTATCCAGATGTGAAATTTGGTGTTGGCCCAGCGATTGAATCTGGTTTCTATTATGATATTGATAC
AGAGGCAGTTATTAGCGATGAATCTCTTGTTGAAATTGAAAAAGAAATGCAAAAAATTGTGCGTGAAAATGTTCCAATTG
AGCGCGAAGTTGTTTCTCGCGAAGAAGCAATTAAACGTTTCAAAGCAATTGGAGATCAATATAAATTAGAACTTATCGAA
GCAATCCCAGAAGATGAAACAGTGACAATTTATACACAAGGTGAATTTTTCGACCTTTGTCGCGGAGTCCATGTTCCTTC
TACTGGTAAAATTCAAGTGTTCAAACTACTTAGCGTGGCTGGTGCATACTGGCGTGGCGATAGTAACAACAAAATGCTAC
AACGCATTTACGGCACAGCTTTCTTCGATAAAAATGGCTTAAAAGAATTTATCCAAATGCAAAAAGAAGCGAAAGAACGT
GATCATCGTAAACTAGGTAAAGAACTAGATTTATTTGCAAATAGTATTGAAGTTGGTCAAGGTTTACCACTTTGGTTGCC
AAAAGGTGCGACTATCCGACGCGTTATCGAACGATACATTGTGGATAAAGAAGAACGCCTTGGTTACAATCACGTTTACA
CTCCGATTATGGCTAATGTTGAGCTTTATAAAACAAGCGGTCACTGGGATCATTACCATGAAGACATGTTCCCAACAATG
AAAATGGACAACGAAGAACTAGTTTTACGTCCAATGAACTGCCCGCATCACATGATGATTTATAAAAATGATATCCATAG
TTACCGTGAACTACCAATCCGTATTGCCGAGCTTGGTATGATGCACCGTTATGAAATGAGTGGGGCGCTTTCAGGACTTC
AACGTGTTCGTGGAATGACTTTAAATGATGCACACGTATTTGTTCGCCCTGACCAAATCAAAGATGAGTTCAAACGGGTT
GTAGAGCTAATTTTAGAAGTATATAAAGACTTTGATATTAAAGATTACTCCTTCCGCCTAAGCTACCGTGATCCGAAAAA
TACTGAAAAATATTTTGACGATGATGCTATGTGGGAAAAAGCGCAAGCCATGCTTAAATCCGCCATGGACGAAATGGAAA
TGGATTACTTTGAAGCAGAAGGTGAAGCTGCATTTTACGGTCCAAAACTAGATGTTCAAGTGAAAACTGCAATAGGAAAA
GAAGAAACACTTTCCACTGTACAACTTGACTTCTTACTTCCAGAACGCTTTGACTTAACTTACATTGGCGAAGATGGCGA
AAAACATCGTCCAGTTGTCATTCACCGTGGAGTTGTATCGACAATGGAACGTTTTGTCGCATATCTAATCGAAGAATACA
AAGGTGCTTTCCCAACTTGGTTAGCTCCAGTACAAATGGAACTTATTCCAGTTAATGCAGATGCACATTTAGATTATGCA
AAAGGTGTACAAGATAAATTACAACGCGCTGGACTTCGTGCTGAAGTGGATGATCGTAATGAAAAATTAGGCTATAAAAT
CCGTGAAGCACAAACAAAGAAAATTCCTTATGCCTTAGTATTAGGTGACCAAGAAGTAGAGGCTGGTTCTGTTAACGTTC
GTCGTTACGGCTCTAAAGACTCTGAAACAATGGATTTAGATGCATTTATCGCACAAGTTGTTGCTGAAGTAAGCAAATAT
TAA

Upstream 100 bases:

>100_bases
GGTGGAACCACGAGCATAAACTCGTCCCTTGACAACAGGAGGGATGGGTTTTTTATTTTGCCCTCCTAACCACACTAAAA
AAAGAAAAGGAGAGATTAGT

Downstream 100 bases:

>100_bases
TCACGAAAATACTGTCTCTTAAAGGGGGCAGTATTTTTTCATGGTGCTTTTTTCCTCTTTTTCCGTTATACTAGTAAAAG
ATAATGAATAGAACAGGATG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MKITFPDGAVKEFEPGVSTADIAASISPGLKKKALAGKLNGELLDLVTPIHEDGAIEIVTPDHEDALGILRHSTAHLMAQ
ALKRLYPDVKFGVGPAIESGFYYDIDTEAVISDESLVEIEKEMQKIVRENVPIEREVVSREEAIKRFKAIGDQYKLELIE
AIPEDETVTIYTQGEFFDLCRGVHVPSTGKIQVFKLLSVAGAYWRGDSNNKMLQRIYGTAFFDKNGLKEFIQMQKEAKER
DHRKLGKELDLFANSIEVGQGLPLWLPKGATIRRVIERYIVDKEERLGYNHVYTPIMANVELYKTSGHWDHYHEDMFPTM
KMDNEELVLRPMNCPHHMMIYKNDIHSYRELPIRIAELGMMHRYEMSGALSGLQRVRGMTLNDAHVFVRPDQIKDEFKRV
VELILEVYKDFDIKDYSFRLSYRDPKNTEKYFDDDAMWEKAQAMLKSAMDEMEMDYFEAEGEAAFYGPKLDVQVKTAIGK
EETLSTVQLDFLLPERFDLTYIGEDGEKHRPVVIHRGVVSTMERFVAYLIEEYKGAFPTWLAPVQMELIPVNADAHLDYA
KGVQDKLQRAGLRAEVDDRNEKLGYKIREAQTKKIPYALVLGDQEVEAGSVNVRRYGSKDSETMDLDAFIAQVVAEVSKY

Sequences:

>Translated_640_residues
MKITFPDGAVKEFEPGVSTADIAASISPGLKKKALAGKLNGELLDLVTPIHEDGAIEIVTPDHEDALGILRHSTAHLMAQ
ALKRLYPDVKFGVGPAIESGFYYDIDTEAVISDESLVEIEKEMQKIVRENVPIEREVVSREEAIKRFKAIGDQYKLELIE
AIPEDETVTIYTQGEFFDLCRGVHVPSTGKIQVFKLLSVAGAYWRGDSNNKMLQRIYGTAFFDKNGLKEFIQMQKEAKER
DHRKLGKELDLFANSIEVGQGLPLWLPKGATIRRVIERYIVDKEERLGYNHVYTPIMANVELYKTSGHWDHYHEDMFPTM
KMDNEELVLRPMNCPHHMMIYKNDIHSYRELPIRIAELGMMHRYEMSGALSGLQRVRGMTLNDAHVFVRPDQIKDEFKRV
VELILEVYKDFDIKDYSFRLSYRDPKNTEKYFDDDAMWEKAQAMLKSAMDEMEMDYFEAEGEAAFYGPKLDVQVKTAIGK
EETLSTVQLDFLLPERFDLTYIGEDGEKHRPVVIHRGVVSTMERFVAYLIEEYKGAFPTWLAPVQMELIPVNADAHLDYA
KGVQDKLQRAGLRAEVDDRNEKLGYKIREAQTKKIPYALVLGDQEVEAGSVNVRRYGSKDSETMDLDAFIAQVVAEVSKY
>Mature_640_residues
MKITFPDGAVKEFEPGVSTADIAASISPGLKKKALAGKLNGELLDLVTPIHEDGAIEIVTPDHEDALGILRHSTAHLMAQ
ALKRLYPDVKFGVGPAIESGFYYDIDTEAVISDESLVEIEKEMQKIVRENVPIEREVVSREEAIKRFKAIGDQYKLELIE
AIPEDETVTIYTQGEFFDLCRGVHVPSTGKIQVFKLLSVAGAYWRGDSNNKMLQRIYGTAFFDKNGLKEFIQMQKEAKER
DHRKLGKELDLFANSIEVGQGLPLWLPKGATIRRVIERYIVDKEERLGYNHVYTPIMANVELYKTSGHWDHYHEDMFPTM
KMDNEELVLRPMNCPHHMMIYKNDIHSYRELPIRIAELGMMHRYEMSGALSGLQRVRGMTLNDAHVFVRPDQIKDEFKRV
VELILEVYKDFDIKDYSFRLSYRDPKNTEKYFDDDAMWEKAQAMLKSAMDEMEMDYFEAEGEAAFYGPKLDVQVKTAIGK
EETLSTVQLDFLLPERFDLTYIGEDGEKHRPVVIHRGVVSTMERFVAYLIEEYKGAFPTWLAPVQMELIPVNADAHLDYA
KGVQDKLQRAGLRAEVDDRNEKLGYKIREAQTKKIPYALVLGDQEVEAGSVNVRRYGSKDSETMDLDAFIAQVVAEVSKY

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=623, Percent_Identity=38.2022471910112, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI38570111, Length=601, Percent_Identity=38.1031613976705, Blast_Score=434, Evalue=1e-121,
Organism=Homo sapiens, GI20070344, Length=654, Percent_Identity=33.6391437308869, Blast_Score=369, Evalue=1e-102,
Organism=Escherichia coli, GI1788013, Length=636, Percent_Identity=42.4528301886792, Blast_Score=550, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71984192, Length=635, Percent_Identity=37.007874015748, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71984184, Length=635, Percent_Identity=37.007874015748, Blast_Score=440, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322112, Length=634, Percent_Identity=35.4889589905363, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6322654, Length=400, Percent_Identity=35.75, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24583839, Length=622, Percent_Identity=37.7813504823151, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24583842, Length=622, Percent_Identity=37.7813504823151, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI28574141, Length=622, Percent_Identity=37.7813504823151, Blast_Score=446, Evalue=1e-125,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73225; Mature: 73225

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITFPDGAVKEFEPGVSTADIAASISPGLKKKALAGKLNGELLDLVTPIHEDGAIEIVT
CEEECCCCCHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEC
PDHEDALGILRHSTAHLMAQALKRLYPDVKFGVGPAIESGFYYDIDTEAVISDESLVEIE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCEEEECCCCEEECCHHHHHHH
KEMQKIVRENVPIEREVVSREEAIKRFKAIGDQYKLELIEAIPEDETVTIYTQGEFFDLC
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEECCCHHHHH
RGVHVPSTGKIQVFKLLSVAGAYWRGDSNNKMLQRIYGTAFFDKNGLKEFIQMQKEAKER
CCCCCCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DHRKLGKELDLFANSIEVGQGLPLWLPKGATIRRVIERYIVDKEERLGYNHVYTPIMANV
HHHHHCHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCHHHCCCCCCCCHHEECE
ELYKTSGHWDHYHEDMFPTMKMDNEELVLRPMNCPHHMMIYKNDIHSYRELPIRIAELGM
EEEECCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECHHHHHHHCCHHHHHHHH
MHRYEMSGALSGLQRVRGMTLNDAHVFVRPDQIKDEFKRVVELILEVYKDFDIKDYSFRL
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
SYRDPKNTEKYFDDDAMWEKAQAMLKSAMDEMEMDYFEAEGEAAFYGPKLDVQVKTAIGK
ECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCC
EETLSTVQLDFLLPERFDLTYIGEDGEKHRPVVIHRGVVSTMERFVAYLIEEYKGAFPTW
CHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCE
LAPVQMELIPVNADAHLDYAKGVQDKLQRAGLRAEVDDRNEKLGYKIREAQTKKIPYALV
ECCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHCCCCEEEE
LGDQEVEAGSVNVRRYGSKDSETMDLDAFIAQVVAEVSKY
ECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKITFPDGAVKEFEPGVSTADIAASISPGLKKKALAGKLNGELLDLVTPIHEDGAIEIVT
CEEECCCCCHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEC
PDHEDALGILRHSTAHLMAQALKRLYPDVKFGVGPAIESGFYYDIDTEAVISDESLVEIE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCEEEECCCCEEECCHHHHHHH
KEMQKIVRENVPIEREVVSREEAIKRFKAIGDQYKLELIEAIPEDETVTIYTQGEFFDLC
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEECCCHHHHH
RGVHVPSTGKIQVFKLLSVAGAYWRGDSNNKMLQRIYGTAFFDKNGLKEFIQMQKEAKER
CCCCCCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DHRKLGKELDLFANSIEVGQGLPLWLPKGATIRRVIERYIVDKEERLGYNHVYTPIMANV
HHHHHCHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCHHHCCCCCCCCHHEECE
ELYKTSGHWDHYHEDMFPTMKMDNEELVLRPMNCPHHMMIYKNDIHSYRELPIRIAELGM
EEEECCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECHHHHHHHCCHHHHHHHH
MHRYEMSGALSGLQRVRGMTLNDAHVFVRPDQIKDEFKRVVELILEVYKDFDIKDYSFRL
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
SYRDPKNTEKYFDDDAMWEKAQAMLKSAMDEMEMDYFEAEGEAAFYGPKLDVQVKTAIGK
ECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCC
EETLSTVQLDFLLPERFDLTYIGEDGEKHRPVVIHRGVVSTMERFVAYLIEEYKGAFPTW
CHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCE
LAPVQMELIPVNADAHLDYAKGVQDKLQRAGLRAEVDDRNEKLGYKIREAQTKKIPYALV
ECCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHCCCCEEEE
LGDQEVEAGSVNVRRYGSKDSETMDLDAFIAQVVAEVSKY
ECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]