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The map label for this gene is grpE

Identifier: 226224075

GI number: 226224075

Start: 1490875

End: 1491450

Strand: Reverse

Name: grpE

Synonym: Lm4b_01484

Alternate gene names: 226224075

Gene position: 1491450-1490875 (Counterclockwise)

Preceding gene: 226224076

Following gene: 226224074

Centisome position: 51.21

GC content: 38.19

Gene sequence:

>576_bases
GTGTCTGAGAAAAAGAACAAAAAAGAAAGACTAGCTGATGAAATCGAACAAGAAGAATTAAACATTCTAGATGAAACAGA
AGAAACTGTTGAAGAAGAGGCTGCGGCTGACACTTTAACAGAAGAACAAGCAAAAATTTTAGAGCTTGAAAACAAACTTG
ATGAAGTAGAAAATCGCTACCTTCGCATGCAAGCGGACTTCGAAAATGTGAAGAAAAGACATATCGCAGACCGTGATGCC
AGTCAGAAATATCGTTCGCAGAGCCTAGCGCAAGATTTACTTCCCGCACTAGACAGTTTTGAAAAAGCACTTGCAACGAC
TTCTGATCAGGAAGAAGTGAAACAAATTTTAAAAGGAATGGAAATGGTATACAACCAAATCCTTGTTGCTTTTGAAAAAG
AAGGTATTGAAGTCATTCCAGCTGTCGGCGAACAATTTGACCCGAACTTCCATCAAGCCGTAATGCAAGATAGTGATGAA
AATGCAGGAAGCAATGAAATCACTGCGGAACTTCAAAAAGGTTATAAACTTAAAGATCGGGTTATACGCCCATCTATGGT
AAAAGTAAATCAATAA

Upstream 100 bases:

>100_bases
TGATGAGCCGAGATTTAACCGATGTGTTAACCAAACTTTATCGTGATAACCAAAATTAGGAAGGCTGAAAAAGCACACGT
TTTTTCAGGAGGTGGCTAAA

Downstream 100 bases:

>100_bases
AAAATAATTACTGAATAACAGGAGGAAATAACAATGAGCAAAATTATCGGAATTGACTTAGGAACAACAAACTCTGCAGT
AGCAGTATTAGAAGGCGGAG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MSEKKNKKERLADEIEQEELNILDETEETVEEEAAADTLTEEQAKILELENKLDEVENRYLRMQADFENVKKRHIADRDA
SQKYRSQSLAQDLLPALDSFEKALATTSDQEEVKQILKGMEMVYNQILVAFEKEGIEVIPAVGEQFDPNFHQAVMQDSDE
NAGSNEITAELQKGYKLKDRVIRPSMVKVNQ

Sequences:

>Translated_191_residues
MSEKKNKKERLADEIEQEELNILDETEETVEEEAAADTLTEEQAKILELENKLDEVENRYLRMQADFENVKKRHIADRDA
SQKYRSQSLAQDLLPALDSFEKALATTSDQEEVKQILKGMEMVYNQILVAFEKEGIEVIPAVGEQFDPNFHQAVMQDSDE
NAGSNEITAELQKGYKLKDRVIRPSMVKVNQ
>Mature_190_residues
SEKKNKKERLADEIEQEELNILDETEETVEEEAAADTLTEEQAKILELENKLDEVENRYLRMQADFENVKKRHIADRDAS
QKYRSQSLAQDLLPALDSFEKALATTSDQEEVKQILKGMEMVYNQILVAFEKEGIEVIPAVGEQFDPNFHQAVMQDSDEN
AGSNEITAELQKGYKLKDRVIRPSMVKVNQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=173, Percent_Identity=30.635838150289, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI40255109, Length=156, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1788967, Length=176, Percent_Identity=34.0909090909091, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17552458, Length=200, Percent_Identity=31, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=163, Percent_Identity=31.9018404907975, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653432, Length=142, Percent_Identity=29.5774647887324, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21935; Mature: 21804

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKKNKKERLADEIEQEELNILDETEETVEEEAAADTLTEEQAKILELENKLDEVENRY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRMQADFENVKKRHIADRDASQKYRSQSLAQDLLPALDSFEKALATTSDQEEVKQILKGM
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EMVYNQILVAFEKEGIEVIPAVGEQFDPNFHQAVMQDSDENAGSNEITAELQKGYKLKDR
HHHHHHHHHHHHHCCCEEHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHH
VIRPSMVKVNQ
HCCCCHHCCCC
>Mature Secondary Structure 
SEKKNKKERLADEIEQEELNILDETEETVEEEAAADTLTEEQAKILELENKLDEVENRY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRMQADFENVKKRHIADRDASQKYRSQSLAQDLLPALDSFEKALATTSDQEEVKQILKGM
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EMVYNQILVAFEKEGIEVIPAVGEQFDPNFHQAVMQDSDENAGSNEITAELQKGYKLKDR
HHHHHHHHHHHHHCCCEEHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHH
VIRPSMVKVNQ
HCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]