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The map label for this gene is glyS

Identifier: 226224059

GI number: 226224059

Start: 1474781

End: 1476847

Strand: Reverse

Name: glyS

Synonym: Lm4b_01468

Alternate gene names: 226224059

Gene position: 1476847-1474781 (Counterclockwise)

Preceding gene: 226224060

Following gene: 226224058

Centisome position: 50.7

GC content: 36.14

Gene sequence:

>2067_bases
ATGAGTAAAGACTTTTTATTAGAAATTGGTTTAGAAGAAATGCCAGCAAAATATGTGACTAGCTCTGTACTACAACTGGA
AAAACGAGTAACTGATTGGTTAAAAGACAACCAAATCGAATTTAAAGAAATTAAAACTTATTCTACACCAAGACGTTTAA
CTGTCCTAGTAGAAGAAATGGCTGAAGAACAAGCTAATCGTGTGGAAGAAGCAAAAGGCCCTGCAAAAAAAATCGCTCTA
GACGACGAAGGCAACTGGTCAAAAGCAGCATTAGGGTTTGCAAAAAGTCAAAAAGTTGCGCCAGAAGATTTAACTTTCCG
CGAAATTAAAGGTGTTGAATATATTTATATTAAAAAAGAAGTAATCGGCGAAAAAACAACGGCGTTACTTCCAAGCTTAG
AAAAAGTAGTAACAAGTATGACTTTCCCAGTAAGTATGCACTGGGGTAGTAATGATTTACGTTATATTCGTCCAATCAAA
TGGCTTATCGCAATGTTTGGCGAAGAAATTATTCCATTTGAAATCACTGGTGTCTCAACTAGTAATACATCCCGTGGACA
TCGTTTCTTAGGAAAAACAGCTACAATCAACCAACCGAGCGACTATCCAAATGCATTATTAGAACAATTTGTCGTTGTTA
ATGCTAGTGAACGCAAACAGGCTATTGTAGAACAATTACGTGAACTAGAAACGATGGAAAATTGGCAAATCAGAGAAGAT
GACGACTTATTAGAAGAAGTAACTAATCTTGTCGAATATCCAACTGTACTAGCAGGTAATTTTGAAAAAGAATATCTGGA
ACTACCCGAAGAAGTTTTAATTACGACGATGAAAGAACACCAACGTTACTTCCCAGTTTTCAGCCAAGAAGGAGAATTGT
TGCCTCATTTTGTAACCGTTCGTAATGGTAACCATGAAAATCTAGATACAGTGGCACGAGGAAACGAAAAAGTACTACGC
GCTCGTTTGTCAGATGCTGATTTCTTCTATCAAGAAGATTTAAAAATAACCATTGATGAAGCAGTTGCTAAATTACAAAA
TATCGTTTTCCATGAAAAATTAGGCACTTTGACAGAAAAAATGAAACGAGTGCAAAAAGTGGCATTAATGCTTGCTGATT
ATTTAAATTGGCAAGAAGAAGATAAACAAGACATTATCCGCTTAACAAATATTTATAAATTTGATTTAGTAACCAATATC
GTTGGTGAATTCCCTGAACTACAAGGTTTAATGGGCGAAAAATATGCATTGTTGCAAGGCGAAAAACCAGCAATCGCAAC
AGCTATTCGTGAACATTATTTACCAAGCTCAGCAGAAGGCGATTTACCACAAACAGACTTAGGTTCACTTATCGCTATTG
CAGATAAATTAGAAACATTAATTGGCTTCTTCTGTGTAAATATTGCACCAACAGGCTCTGCTGATCCATTTGGTTTACGT
CGTAGTGCTTTTGGAGCGGTGCGTATTATCCAAGCAAATGGTTGGGACATTCCAATGCTTGAAGTGGTTTCTCGTATTGT
GGATATGGAGCGTGCAGAAGGGGCTACAGAACTACCAAGTTCAGATGTAATAAAAGAAGTGCAGACTTTCCTAAAAAATC
GTTTGCGTGTTATTTTACAAGGCCATCATATTCGCCATGATATTATTGACGCTGTTATCGGCGGCGATCCTAATATGATT
CCTCAATTGATTGATCGTGCTCAAATATTGAATAAGCATGCGGACGCAGAGTGGTTCCGCCCAACTATCGAAGCCTTAAC
ACGAGTAGTTAATATTTCTAAAAAATACGAAGATGGCGTAGAAGTGGATCCGAGCCTATTTGAAAATGAATACGAGCAAG
CCCTATTTGATAAATTAGAAAAACTAAAATTTGATTTTGCTGGTTTAAAAATTGTTGACAGACTAAAAGCATTTGCAGAT
TTACGTACAACAATTGATGCATATTTCGATAACACGCTAGTTATGACTGATAATGATGAATTGAAAAATAATCGTCTTGC
ACTATTATTCGAACTTGCAAGCTTTATTAAAGAGTTTGCTCAAATGGATGAAATTAATGTAAAATAA

Upstream 100 bases:

>100_bases
GGTCGTATCAGAAACTTAGCAAGACGTATCGCCAAAACATTTTATGAATCACGAGAAAAACTTGGCTTCCCATTAGTCAA
AGAAGAGGGAGGAAAACGTC

Downstream 100 bases:

>100_bases
AAGGAAGATTTTGAGCAAAGAGGAGGAAGTATGTATGACTCAACCAGCCGTATACGTGGTTTCCGATTCAACAGGAGAAA
CGGCCGAACTAGTGACAAGA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MSKDFLLEIGLEEMPAKYVTSSVLQLEKRVTDWLKDNQIEFKEIKTYSTPRRLTVLVEEMAEEQANRVEEAKGPAKKIAL
DDEGNWSKAALGFAKSQKVAPEDLTFREIKGVEYIYIKKEVIGEKTTALLPSLEKVVTSMTFPVSMHWGSNDLRYIRPIK
WLIAMFGEEIIPFEITGVSTSNTSRGHRFLGKTATINQPSDYPNALLEQFVVVNASERKQAIVEQLRELETMENWQIRED
DDLLEEVTNLVEYPTVLAGNFEKEYLELPEEVLITTMKEHQRYFPVFSQEGELLPHFVTVRNGNHENLDTVARGNEKVLR
ARLSDADFFYQEDLKITIDEAVAKLQNIVFHEKLGTLTEKMKRVQKVALMLADYLNWQEEDKQDIIRLTNIYKFDLVTNI
VGEFPELQGLMGEKYALLQGEKPAIATAIREHYLPSSAEGDLPQTDLGSLIAIADKLETLIGFFCVNIAPTGSADPFGLR
RSAFGAVRIIQANGWDIPMLEVVSRIVDMERAEGATELPSSDVIKEVQTFLKNRLRVILQGHHIRHDIIDAVIGGDPNMI
PQLIDRAQILNKHADAEWFRPTIEALTRVVNISKKYEDGVEVDPSLFENEYEQALFDKLEKLKFDFAGLKIVDRLKAFAD
LRTTIDAYFDNTLVMTDNDELKNNRLALLFELASFIKEFAQMDEINVK

Sequences:

>Translated_688_residues
MSKDFLLEIGLEEMPAKYVTSSVLQLEKRVTDWLKDNQIEFKEIKTYSTPRRLTVLVEEMAEEQANRVEEAKGPAKKIAL
DDEGNWSKAALGFAKSQKVAPEDLTFREIKGVEYIYIKKEVIGEKTTALLPSLEKVVTSMTFPVSMHWGSNDLRYIRPIK
WLIAMFGEEIIPFEITGVSTSNTSRGHRFLGKTATINQPSDYPNALLEQFVVVNASERKQAIVEQLRELETMENWQIRED
DDLLEEVTNLVEYPTVLAGNFEKEYLELPEEVLITTMKEHQRYFPVFSQEGELLPHFVTVRNGNHENLDTVARGNEKVLR
ARLSDADFFYQEDLKITIDEAVAKLQNIVFHEKLGTLTEKMKRVQKVALMLADYLNWQEEDKQDIIRLTNIYKFDLVTNI
VGEFPELQGLMGEKYALLQGEKPAIATAIREHYLPSSAEGDLPQTDLGSLIAIADKLETLIGFFCVNIAPTGSADPFGLR
RSAFGAVRIIQANGWDIPMLEVVSRIVDMERAEGATELPSSDVIKEVQTFLKNRLRVILQGHHIRHDIIDAVIGGDPNMI
PQLIDRAQILNKHADAEWFRPTIEALTRVVNISKKYEDGVEVDPSLFENEYEQALFDKLEKLKFDFAGLKIVDRLKAFAD
LRTTIDAYFDNTLVMTDNDELKNNRLALLFELASFIKEFAQMDEINVK
>Mature_687_residues
SKDFLLEIGLEEMPAKYVTSSVLQLEKRVTDWLKDNQIEFKEIKTYSTPRRLTVLVEEMAEEQANRVEEAKGPAKKIALD
DEGNWSKAALGFAKSQKVAPEDLTFREIKGVEYIYIKKEVIGEKTTALLPSLEKVVTSMTFPVSMHWGSNDLRYIRPIKW
LIAMFGEEIIPFEITGVSTSNTSRGHRFLGKTATINQPSDYPNALLEQFVVVNASERKQAIVEQLRELETMENWQIREDD
DLLEEVTNLVEYPTVLAGNFEKEYLELPEEVLITTMKEHQRYFPVFSQEGELLPHFVTVRNGNHENLDTVARGNEKVLRA
RLSDADFFYQEDLKITIDEAVAKLQNIVFHEKLGTLTEKMKRVQKVALMLADYLNWQEEDKQDIIRLTNIYKFDLVTNIV
GEFPELQGLMGEKYALLQGEKPAIATAIREHYLPSSAEGDLPQTDLGSLIAIADKLETLIGFFCVNIAPTGSADPFGLRR
SAFGAVRIIQANGWDIPMLEVVSRIVDMERAEGATELPSSDVIKEVQTFLKNRLRVILQGHHIRHDIIDAVIGGDPNMIP
QLIDRAQILNKHADAEWFRPTIEALTRVVNISKKYEDGVEVDPSLFENEYEQALFDKLEKLKFDFAGLKIVDRLKAFADL
RTTIDAYFDNTLVMTDNDELKNNRLALLFELASFIKEFAQMDEINVK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=681, Percent_Identity=33.920704845815, Blast_Score=364, Evalue=1e-101,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 78503; Mature: 78372

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDFLLEIGLEEMPAKYVTSSVLQLEKRVTDWLKDNQIEFKEIKTYSTPRRLTVLVEEM
CCCCEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHH
AEEQANRVEEAKGPAKKIALDDEGNWSKAALGFAKSQKVAPEDLTFREIKGVEYIYIKKE
HHHHHHHHHHHCCCHHEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEEHH
VIGEKTTALLPSLEKVVTSMTFPVSMHWGSNDLRYIRPIKWLIAMFGEEIIPFEITGVST
HHCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCC
SNTSRGHRFLGKTATINQPSDYPNALLEQFVVVNASERKQAIVEQLRELETMENWQIRED
CCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCH
DDLLEEVTNLVEYPTVLAGNFEKEYLELPEEVLITTMKEHQRYFPVFSQEGELLPHFVTV
HHHHHHHHHHHHCCHHHCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
RNGNHENLDTVARGNEKVLRARLSDADFFYQEDLKITIDEAVAKLQNIVFHEKLGTLTEK
ECCCCCCHHHHHCCCHHHHHHHHCCCHHEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
MKRVQKVALMLADYLNWQEEDKQDIIRLTNIYKFDLVTNIVGEFPELQGLMGEKYALLQG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECC
EKPAIATAIREHYLPSSAEGDLPQTDLGSLIAIADKLETLIGFFCVNIAPTGSADPFGLR
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
RSAFGAVRIIQANGWDIPMLEVVSRIVDMERAEGATELPSSDVIKEVQTFLKNRLRVILQ
HHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GHHIRHDIIDAVIGGDPNMIPQLIDRAQILNKHADAEWFRPTIEALTRVVNISKKYEDGV
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EVDPSLFENEYEQALFDKLEKLKFDFAGLKIVDRLKAFADLRTTIDAYFDNTLVMTDNDE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCH
LKNNRLALLFELASFIKEFAQMDEINVK
HCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKDFLLEIGLEEMPAKYVTSSVLQLEKRVTDWLKDNQIEFKEIKTYSTPRRLTVLVEEM
CCCEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHH
AEEQANRVEEAKGPAKKIALDDEGNWSKAALGFAKSQKVAPEDLTFREIKGVEYIYIKKE
HHHHHHHHHHHCCCHHEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEEHH
VIGEKTTALLPSLEKVVTSMTFPVSMHWGSNDLRYIRPIKWLIAMFGEEIIPFEITGVST
HHCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCC
SNTSRGHRFLGKTATINQPSDYPNALLEQFVVVNASERKQAIVEQLRELETMENWQIRED
CCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCH
DDLLEEVTNLVEYPTVLAGNFEKEYLELPEEVLITTMKEHQRYFPVFSQEGELLPHFVTV
HHHHHHHHHHHHCCHHHCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
RNGNHENLDTVARGNEKVLRARLSDADFFYQEDLKITIDEAVAKLQNIVFHEKLGTLTEK
ECCCCCCHHHHHCCCHHHHHHHHCCCHHEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
MKRVQKVALMLADYLNWQEEDKQDIIRLTNIYKFDLVTNIVGEFPELQGLMGEKYALLQG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECC
EKPAIATAIREHYLPSSAEGDLPQTDLGSLIAIADKLETLIGFFCVNIAPTGSADPFGLR
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
RSAFGAVRIIQANGWDIPMLEVVSRIVDMERAEGATELPSSDVIKEVQTFLKNRLRVILQ
HHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GHHIRHDIIDAVIGGDPNMIPQLIDRAQILNKHADAEWFRPTIEALTRVVNISKKYEDGV
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EVDPSLFENEYEQALFDKLEKLKFDFAGLKIVDRLKAFADLRTTIDAYFDNTLVMTDNDE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCH
LKNNRLALLFELASFIKEFAQMDEINVK
HCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]