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The map label for this gene is dnaG

Identifier: 226224056

GI number: 226224056

Start: 1471394

End: 1473274

Strand: Reverse

Name: dnaG

Synonym: Lm4b_01465

Alternate gene names: 226224056

Gene position: 1473274-1471394 (Counterclockwise)

Preceding gene: 226224057

Following gene: 226224055

Centisome position: 50.58

GC content: 37.69

Gene sequence:

>1881_bases
ATGGCGCGGATTCCTGAAGAAGTAATTGATCAAGTCCGGAATCAAGCGGATATAGTCGATATTATCGGTAATTATGTTCA
GTTAAAAAAACAAGGACGTAATTATTCTGGCTTATGTCCTTTCCACGGTGAAAAAACGCCATCCTTTTCTGTCTCACCAG
AAAAACAGATGTTCCATTGTTTTGGTTGTGGTAAGGGAGGGAATGTTTTTTCTTTTCTAATGGAGCATGACGGACTCACT
TTTGTTGAATCGGTAAAAAAAGTGGCAGACATGAGTCATTTAGATGTGGCTATCGAGCTCCCTGAAGAACGAGATACAAG
TAATTTGCCAAAAGAAACTTCTGAAACAGCAAAAATGGTGGAAATGCACCAGCTTACTGCCAAACTTTACCATTATATTT
TAATGGAAACTGAAGAAGGTACAGCAGCATTAACTTATTTAAAAGAGCGTGGTATGTCTGAACAGATGATGACCACTTTC
CAAATTGGTTTTGCCCCTAATCACCATGCAACAATTACTGCTTTTCTAGAAAAACGTGGCATGGATTTACAATTAGCTGG
CAGGGCTGGACTTTTGTCCGAACGTGATGATGGGCAAATGGTGGATCGTTTTCGTAATCGGATTATGTTTCCAATTACGA
ATGATCGTGGACAAATTATTGCCTTTTCTGGCCGCTTATTTGATCGTGATGATGGTCCTAAGTATTTAAATAGCCCTGAA
ACACCTGTTTTCAATAAAAGACGAATATTGTTTCATTTTTCAGAAGCAAGACAAGCTATTAGAAAACAAGAAGAAATTAC
GCTCATGGAAGGATTTATGGATGTTATTTCCGCAGAAGAAGCAGGTGTTCAAAATGCGGTAGCTTCGATGGGGACAAGCT
TAACGGAAGAACATGCAGATTTAATTAAACGGCTTACTAACCGGGCGATTATTTGTTATGACGGTGACAGAGCCGGAATT
GAAGCAGCCTATAAGGCGGGCACACTTCTAGTTGAACGGAATCGTTTAGATGTTTTTGTTTTGCAACTTCCAGCTGGAAA
AGATCCCGATGACTTTATTCGAGCAAGTGGTCCAGAAAAATTCAAAGAAGTTTATAAGCAACAACGAATGACTTGGACAG
CTTTTAAAATTCATTATTTACGTAGAGAACGTAATTTACAGAATGAGACAGATCAAATTGGTTATATTGATGATTGTTTA
CGCGAAATTGCGAAACTAGATCAAGCCGTCGAACGCGAATTATACTTAAAACAACTAGCAGATGAATTTGAATTAACGAT
AGAAACATTAAAACAACAACTACAGCAATCACTAAAAAATAGTCAGAAATCACGACAAATGGCTAGTTATAATGAACCAC
CAATAGATGATTCTTTTATGGGAATGATGCCTCAAGAAGAAACAGAAATGCTATTTTCTTTCGAGCAACCAACCCAAAAA
CTATCTGCCCATACAACCTCAGAGCAACAACTCATGAAAGCAATGATGGAGAGCCGAGATAATTTTCTTTTAATTAAGCA
ACTTCTTGGTGACACTACGTTTTATCATGATAATTACAAAGCACTTTATACCTATCTAATTGGTTATTTTGCAGAAGGTA
ATGATGCAGATCCAACGAAATTTATGGATAGCGTTCCTGATGCTACAATGAAAGGACTTATCAGTAGCCTCGAAATGGTT
ATTAGTCCCGACGAACAAGGGAAAACCCAGTTTGAAGACTATATTAGAAGTCTAAAGCGGTTTAAATTAGAACAAAAGAA
AAAAGAACTTGAGCAAGAGCTAGCGACTTTAAATCGTGAAAATGACAAAGATAACGAAATTCGTGTCATGCTCGAAATCG
TCCAACTCAACCGTCAGTTAAACAGCGGCCAATTGGATTAA

Upstream 100 bases:

>100_bases
AGCAAAGGTCCAAAAGCAATGAGCGATCAAGATCGTTCTCTTTTCAAAGACCGATTGACGACATTTTTGCAAAATAAGTA
AATAGGAGTTGACTGTCAAA

Downstream 100 bases:

>100_bases
TAACGTTTTAAAACCGCTAAATGATGGTATTATTACCTAAGAGAAGCCTTTTAATAAGGTTAGCGGCATTTTGGAAGGAG
GAATACAGGCAGTTATGAGT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MARIPEEVIDQVRNQADIVDIIGNYVQLKKQGRNYSGLCPFHGEKTPSFSVSPEKQMFHCFGCGKGGNVFSFLMEHDGLT
FVESVKKVADMSHLDVAIELPEERDTSNLPKETSETAKMVEMHQLTAKLYHYILMETEEGTAALTYLKERGMSEQMMTTF
QIGFAPNHHATITAFLEKRGMDLQLAGRAGLLSERDDGQMVDRFRNRIMFPITNDRGQIIAFSGRLFDRDDGPKYLNSPE
TPVFNKRRILFHFSEARQAIRKQEEITLMEGFMDVISAEEAGVQNAVASMGTSLTEEHADLIKRLTNRAIICYDGDRAGI
EAAYKAGTLLVERNRLDVFVLQLPAGKDPDDFIRASGPEKFKEVYKQQRMTWTAFKIHYLRRERNLQNETDQIGYIDDCL
REIAKLDQAVERELYLKQLADEFELTIETLKQQLQQSLKNSQKSRQMASYNEPPIDDSFMGMMPQEETEMLFSFEQPTQK
LSAHTTSEQQLMKAMMESRDNFLLIKQLLGDTTFYHDNYKALYTYLIGYFAEGNDADPTKFMDSVPDATMKGLISSLEMV
ISPDEQGKTQFEDYIRSLKRFKLEQKKKELEQELATLNRENDKDNEIRVMLEIVQLNRQLNSGQLD

Sequences:

>Translated_626_residues
MARIPEEVIDQVRNQADIVDIIGNYVQLKKQGRNYSGLCPFHGEKTPSFSVSPEKQMFHCFGCGKGGNVFSFLMEHDGLT
FVESVKKVADMSHLDVAIELPEERDTSNLPKETSETAKMVEMHQLTAKLYHYILMETEEGTAALTYLKERGMSEQMMTTF
QIGFAPNHHATITAFLEKRGMDLQLAGRAGLLSERDDGQMVDRFRNRIMFPITNDRGQIIAFSGRLFDRDDGPKYLNSPE
TPVFNKRRILFHFSEARQAIRKQEEITLMEGFMDVISAEEAGVQNAVASMGTSLTEEHADLIKRLTNRAIICYDGDRAGI
EAAYKAGTLLVERNRLDVFVLQLPAGKDPDDFIRASGPEKFKEVYKQQRMTWTAFKIHYLRRERNLQNETDQIGYIDDCL
REIAKLDQAVERELYLKQLADEFELTIETLKQQLQQSLKNSQKSRQMASYNEPPIDDSFMGMMPQEETEMLFSFEQPTQK
LSAHTTSEQQLMKAMMESRDNFLLIKQLLGDTTFYHDNYKALYTYLIGYFAEGNDADPTKFMDSVPDATMKGLISSLEMV
ISPDEQGKTQFEDYIRSLKRFKLEQKKKELEQELATLNRENDKDNEIRVMLEIVQLNRQLNSGQLD
>Mature_625_residues
ARIPEEVIDQVRNQADIVDIIGNYVQLKKQGRNYSGLCPFHGEKTPSFSVSPEKQMFHCFGCGKGGNVFSFLMEHDGLTF
VESVKKVADMSHLDVAIELPEERDTSNLPKETSETAKMVEMHQLTAKLYHYILMETEEGTAALTYLKERGMSEQMMTTFQ
IGFAPNHHATITAFLEKRGMDLQLAGRAGLLSERDDGQMVDRFRNRIMFPITNDRGQIIAFSGRLFDRDDGPKYLNSPET
PVFNKRRILFHFSEARQAIRKQEEITLMEGFMDVISAEEAGVQNAVASMGTSLTEEHADLIKRLTNRAIICYDGDRAGIE
AAYKAGTLLVERNRLDVFVLQLPAGKDPDDFIRASGPEKFKEVYKQQRMTWTAFKIHYLRRERNLQNETDQIGYIDDCLR
EIAKLDQAVERELYLKQLADEFELTIETLKQQLQQSLKNSQKSRQMASYNEPPIDDSFMGMMPQEETEMLFSFEQPTQKL
SAHTTSEQQLMKAMMESRDNFLLIKQLLGDTTFYHDNYKALYTYLIGYFAEGNDADPTKFMDSVPDATMKGLISSLEMVI
SPDEQGKTQFEDYIRSLKRFKLEQKKKELEQELATLNRENDKDNEIRVMLEIVQLNRQLNSGQLD

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=383, Percent_Identity=40.7310704960835, Blast_Score=284, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 71946; Mature: 71815

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIPEEVIDQVRNQADIVDIIGNYVQLKKQGRNYSGLCPFHGEKTPSFSVSPEKQMFHC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHEEE
FGCGKGGNVFSFLMEHDGLTFVESVKKVADMSHLDVAIELPEERDTSNLPKETSETAKMV
ECCCCCCHHHHHHHHCCCCHHHHHHHHHHCHHHCCEEEECCCCCCCCCCCCHHHHHHHHH
EMHQLTAKLYHYILMETEEGTAALTYLKERGMSEQMMTTFQIGFAPNHHATITAFLEKRG
HHHHHHHHHHHHHHEECCCCHHHHHHHHHCCCHHHHHHHEEECCCCCCCHHHHHHHHHCC
MDLQLAGRAGLLSERDDGQMVDRFRNRIMFPITNDRGQIIAFSGRLFDRDDGPKYLNSPE
CCEEECCCCCCCCCCCCHHHHHHHHCCEEEEEECCCCCEEEEECEEECCCCCCCCCCCCC
TPVFNKRRILFHFSEARQAIRKQEEITLMEGFMDVISAEEAGVQNAVASMGTSLTEEHAD
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LIKRLTNRAIICYDGDRAGIEAAYKAGTLLVERNRLDVFVLQLPAGKDPDDFIRASGPEK
HHHHHHCCEEEEECCCCCCHHHHHHCCEEEEECCCCEEEEEECCCCCCHHHHHHCCCHHH
FKEVYKQQRMTWTAFKIHYLRRERNLQNETDQIGYIDDCLREIAKLDQAVERELYLKQLA
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DEFELTIETLKQQLQQSLKNSQKSRQMASYNEPPIDDSFMGMMPQEETEMLFSFEQPTQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHH
LSAHTTSEQQLMKAMMESRDNFLLIKQLLGDTTFYHDNYKALYTYLIGYFAEGNDADPTK
HHHCCCHHHHHHHHHHHCCCCEEHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCCCHHH
FMDSVPDATMKGLISSLEMVISPDEQGKTQFEDYIRSLKRFKLEQKKKELEQELATLNRE
HHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NDKDNEIRVMLEIVQLNRQLNSGQLD
CCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARIPEEVIDQVRNQADIVDIIGNYVQLKKQGRNYSGLCPFHGEKTPSFSVSPEKQMFHC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHEEE
FGCGKGGNVFSFLMEHDGLTFVESVKKVADMSHLDVAIELPEERDTSNLPKETSETAKMV
ECCCCCCHHHHHHHHCCCCHHHHHHHHHHCHHHCCEEEECCCCCCCCCCCCHHHHHHHHH
EMHQLTAKLYHYILMETEEGTAALTYLKERGMSEQMMTTFQIGFAPNHHATITAFLEKRG
HHHHHHHHHHHHHHEECCCCHHHHHHHHHCCCHHHHHHHEEECCCCCCCHHHHHHHHHCC
MDLQLAGRAGLLSERDDGQMVDRFRNRIMFPITNDRGQIIAFSGRLFDRDDGPKYLNSPE
CCEEECCCCCCCCCCCCHHHHHHHHCCEEEEEECCCCCEEEEECEEECCCCCCCCCCCCC
TPVFNKRRILFHFSEARQAIRKQEEITLMEGFMDVISAEEAGVQNAVASMGTSLTEEHAD
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LIKRLTNRAIICYDGDRAGIEAAYKAGTLLVERNRLDVFVLQLPAGKDPDDFIRASGPEK
HHHHHHCCEEEEECCCCCCHHHHHHCCEEEEECCCCEEEEEECCCCCCHHHHHHCCCHHH
FKEVYKQQRMTWTAFKIHYLRRERNLQNETDQIGYIDDCLREIAKLDQAVERELYLKQLA
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DEFELTIETLKQQLQQSLKNSQKSRQMASYNEPPIDDSFMGMMPQEETEMLFSFEQPTQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHH
LSAHTTSEQQLMKAMMESRDNFLLIKQLLGDTTFYHDNYKALYTYLIGYFAEGNDADPTK
HHHCCCHHHHHHHHHHHCCCCEEHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCCCHHH
FMDSVPDATMKGLISSLEMVISPDEQGKTQFEDYIRSLKRFKLEQKKKELEQELATLNRE
HHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NDKDNEIRVMLEIVQLNRQLNSGQLD
CCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]