Click here to switch to the map view.

The map label for this gene is ispH

Identifier: 226224052

GI number: 226224052

Start: 1467244

End: 1468239

Strand: Direct

Name: ispH

Synonym: Lm4b_01461

Alternate gene names: 226224052

Gene position: 1467244-1468239 (Clockwise)

Preceding gene: 226224044

Following gene: 226224062

Centisome position: 50.37

GC content: 39.76

Gene sequence:

>996_bases
ATGGAAATTATCAAAATATCTCCTCGTGGTTATTGCTACGGAGTCATCGATGCGATGGTGATTGCTAAAAATGCATCACT
TGATCCGAACTTGCCTCGACCAATTCATATTTTAGGTATGATTGTTCATAATAAACATGTTACTGATGCATTTGAATCCA
TTGGTATTTATACAGTAGATGGCGCTAATCGAGAAGAAATTTTAGATAAAATAACCACTGGAACGGTTATATTTACCGCA
CATGGTGTCTCTCCTTCTGTCAAAGCGAAGGCAGCAGCAAAAGGCTTAACGACAATCGACGCAACCTGCCCAGATGTACT
TCATACCTATAATTTAATTTTAGAAAAACAAGCGGCTGGCTATGAAATTATTTATATAGGAAAAAAAGGACATCCTGAAC
CAGAAGGCGCATACGGAACAGCGCCTGATGTTGTTCACCTAGTAGAAACAAAAGCAGACATTGATGCTCTTTCATTACTT
TCCGATAACATTTTTGTGACGAACCAAACAACGATGAGCAAATGGGATGTCGCTGATTTAATGGATTATATAAAAGAAAA
ATTCCCGAAAGCTATTCAACATCAAGAAATATGTATGGCCACTCAAGTGCGGCAAGAAGCTGTTGCAGTACAAGCCAAAG
GTGCTGATCTCACTATTGTTGTCGGCGATCCTAGAAGTAATAATACTGCTCGTTTAGCCCAAGTTTCGATAGAGAAAGCT
GGCACAAAGGCGTACCGAATTGCCGATATTACGGAATTAGATATAGAGTGGATCAAAGACGCAAAAAAAGTAGCAGTTAC
AGCCGGAGCAAGTACGCCAACACAACTTGTTCGTGAAGTGCTATTGTTTTTGGAGCAGTTTGATGCGGCAGATAAAACTA
CTTGGAAACGTGCGCATAATAAGGACTTTGAGCGTATTCTTCCCAAAATGAAGAATAAGTACATGGCTGAAAAAAGACGT
CAACGGCTAGCTCACTTAAAAAATGGCGGAAGCTAA

Upstream 100 bases:

>100_bases
AATTGGACATTCGTGCGCGTGTTAAGACAACTTTGTCAAAAACAGGCTAAGTCTATTATACTAAATAAAGAATCTAAAAC
AGAAATGAGGATGTTTTCGA

Downstream 100 bases:

>100_bases
AAACCGAGCAACTCCTTTATTTAAGGGAGTTACTCGGTTTTTTTATCAACAAAATTGGAATGGATCTGTATTTACTTCTG
ATACAATAAATTCTGCTTCA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MEIIKISPRGYCYGVIDAMVIAKNASLDPNLPRPIHILGMIVHNKHVTDAFESIGIYTVDGANREEILDKITTGTVIFTA
HGVSPSVKAKAAAKGLTTIDATCPDVLHTYNLILEKQAAGYEIIYIGKKGHPEPEGAYGTAPDVVHLVETKADIDALSLL
SDNIFVTNQTTMSKWDVADLMDYIKEKFPKAIQHQEICMATQVRQEAVAVQAKGADLTIVVGDPRSNNTARLAQVSIEKA
GTKAYRIADITELDIEWIKDAKKVAVTAGASTPTQLVREVLLFLEQFDAADKTTWKRAHNKDFERILPKMKNKYMAEKRR
QRLAHLKNGGS

Sequences:

>Translated_331_residues
MEIIKISPRGYCYGVIDAMVIAKNASLDPNLPRPIHILGMIVHNKHVTDAFESIGIYTVDGANREEILDKITTGTVIFTA
HGVSPSVKAKAAAKGLTTIDATCPDVLHTYNLILEKQAAGYEIIYIGKKGHPEPEGAYGTAPDVVHLVETKADIDALSLL
SDNIFVTNQTTMSKWDVADLMDYIKEKFPKAIQHQEICMATQVRQEAVAVQAKGADLTIVVGDPRSNNTARLAQVSIEKA
GTKAYRIADITELDIEWIKDAKKVAVTAGASTPTQLVREVLLFLEQFDAADKTTWKRAHNKDFERILPKMKNKYMAEKRR
QRLAHLKNGGS
>Mature_331_residues
MEIIKISPRGYCYGVIDAMVIAKNASLDPNLPRPIHILGMIVHNKHVTDAFESIGIYTVDGANREEILDKITTGTVIFTA
HGVSPSVKAKAAAKGLTTIDATCPDVLHTYNLILEKQAAGYEIIYIGKKGHPEPEGAYGTAPDVVHLVETKADIDALSLL
SDNIFVTNQTTMSKWDVADLMDYIKEKFPKAIQHQEICMATQVRQEAVAVQAKGADLTIVVGDPRSNNTARLAQVSIEKA
GTKAYRIADITELDIEWIKDAKKVAVTAGASTPTQLVREVLLFLEQFDAADKTTWKRAHNKDFERILPKMKNKYMAEKRR
QRLAHLKNGGS

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=316, Percent_Identity=35.7594936708861, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_LISMC (C1KV98)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002758159.1
- GeneID:   7704217
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_01461
- OMA:   MDVPGFY
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 36477; Mature: 36477

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIIKISPRGYCYGVIDAMVIAKNASLDPNLPRPIHILGMIVHNKHVTDAFESIGIYTVD
CCEEEECCCCEEHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCEEEEC
GANREEILDKITTGTVIFTAHGVSPSVKAKAAAKGLTTIDATCPDVLHTYNLILEKQAAG
CCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCCC
YEIIYIGKKGHPEPEGAYGTAPDVVHLVETKADIDALSLLSDNIFVTNQTTMSKWDVADL
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCEEEECCCCCCCCCHHHH
MDYIKEKFPKAIQHQEICMATQVRQEAVAVQAKGADLTIVVGDPRSNNTARLAQVSIEKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCCCCCCEEEEEHHHHC
GTKAYRIADITELDIEWIKDAKKVAVTAGASTPTQLVREVLLFLEQFDAADKTTWKRAHN
CCCEEEEECCHHHHHHHHHCCHHEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
KDFERILPKMKNKYMAEKRRQRLAHLKNGGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEIIKISPRGYCYGVIDAMVIAKNASLDPNLPRPIHILGMIVHNKHVTDAFESIGIYTVD
CCEEEECCCCEEHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCEEEEC
GANREEILDKITTGTVIFTAHGVSPSVKAKAAAKGLTTIDATCPDVLHTYNLILEKQAAG
CCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCCC
YEIIYIGKKGHPEPEGAYGTAPDVVHLVETKADIDALSLLSDNIFVTNQTTMSKWDVADL
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCEEEECCCCCCCCCHHHH
MDYIKEKFPKAIQHQEICMATQVRQEAVAVQAKGADLTIVVGDPRSNNTARLAQVSIEKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCCCCCCEEEEEHHHHC
GTKAYRIADITELDIEWIKDAKKVAVTAGASTPTQLVREVLLFLEQFDAADKTTWKRAHN
CCCEEEEECCHHHHHHHHHCCHHEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
KDFERILPKMKNKYMAEKRRQRLAHLKNGGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA