Click here to switch to the map view.

The map label for this gene is thlA [H]

Identifier: 226224015

GI number: 226224015

Start: 1427568

End: 1428737

Strand: Reverse

Name: thlA [H]

Synonym: Lm4b_01423

Alternate gene names: 226224015

Gene position: 1428737-1427568 (Counterclockwise)

Preceding gene: 226224017

Following gene: 226224014

Centisome position: 49.05

GC content: 40.34

Gene sequence:

>1170_bases
ATGAACGAAGTAGTTATAATAGATGCAGTTAGAACACCAATTGGAAAATTTGGTGGTAGTTTGAAAGACATCAGTGCAGT
AGACCTTGGCGCTACCGCTCTAAAAGGTGTTTTAGAAAGAGCCAATATTGCTCCAGAACGGGTGGATCAAGTGATTTTTG
GTAATGTATTACAAGCTGGACTTGGCCAAAATGTAGCTAGACAAATCGCTATTAAAGCAGGAATTCCTTATAAAGTACCC
GGAGTCACAATCAATGAAGTTTGTGGATCTGGCTTAAAATCGGTTATGCTTGGTAGGCAAGCAATTCAGCTAGGGGAGGC
TGACATCGTAGCCGTTGGTGGGACCGAAAATATGTCCCAAGCACCACTTTTACTTAATCCCGAGCTAGCTGGCGAAGAAA
TCGATCCAAAAAAACTAAGAAATAGCATGTTAATTGATGGTTTAACAGATGTTTACGGCGAGTATCACATGGGAATTACT
GCAGAAAATGTTGCAGAGAAATTCTCCGTAACCAGAGAAGAACAAGATGAATTTGCCCATAACTCACAAATGAAGGCCGC
GAATGCACAAGAAAAAAACCTTTTTGAAGAGGAAATTATTCCTGTAAAGCTTCCAGACGGTTCATTTTTTGAAGCCGATG
AAACGATTCGCGCGAATTCAACTCTAGAAAAATTAGCTACTTTGAAAAGCGTATTCAAAGAAGGTGGAACGGTTACAGCC
GGCAATGCTTCCGGTATCAATGATGGAGCTTCCGCAATTATTTTAATGTCGAAAGAAAAGGCAGTCGCGGAAAACATTCC
TTATATCGCAACAATCAAAGTAACCTCTGAAGTTGGTGTCGATCCAGCACTTATGGGTTACGCGCCATACTATGCAGTAA
ATGAAGCTTTAACTAAAGGTGGATATTCTATCGATGACATTGATTTATTCCATTTAAACGAAGCTTTTGCATCGCAGTCA
GTTGCAGTGGCTCGTGATTTAAAAATTCCAGAAGAGAAACTTAATATTTACGGTGGAGCAATTGCACTAGGCCATCCAAT
TGGAGCTTCTGGTGCACGCATTATTGCCTCATTGTTAAATGAGTTAAAACATGAAAATAAGCACATCGGTGTCGCTTCCT
TGTGTGTTGGTGGTGGTATTGGTATTGCAATTATCCTTGAAAAAGCTTGA

Upstream 100 bases:

>100_bases
TAGAAAAATGCATAAAATACAATACATTGGGCTTAATCTAGTGAGTTTTTAGCTAGTTTCATGATATAATACGGATAGAT
TACATTTAAAGGAGATTGCT

Downstream 100 bases:

>100_bases
TAGTAGTACATTTAATGTTAGTAAAAAGCTAAAATCAGCTAATAGTTGATTTTAGCTTTTTATGATTTCATTTTTGTCTT
TTTTGTGAACAAACCGCCAA

Product: Acetyl-CoA:acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNEVVIIDAVRTPIGKFGGSLKDISAVDLGATALKGVLERANIAPERVDQVIFGNVLQAGLGQNVARQIAIKAGIPYKVP
GVTINEVCGSGLKSVMLGRQAIQLGEADIVAVGGTENMSQAPLLLNPELAGEEIDPKKLRNSMLIDGLTDVYGEYHMGIT
AENVAEKFSVTREEQDEFAHNSQMKAANAQEKNLFEEEIIPVKLPDGSFFEADETIRANSTLEKLATLKSVFKEGGTVTA
GNASGINDGASAIILMSKEKAVAENIPYIATIKVTSEVGVDPALMGYAPYYAVNEALTKGGYSIDDIDLFHLNEAFASQS
VAVARDLKIPEEKLNIYGGAIALGHPIGASGARIIASLLNELKHENKHIGVASLCVGGGIGIAIILEKA

Sequences:

>Translated_389_residues
MNEVVIIDAVRTPIGKFGGSLKDISAVDLGATALKGVLERANIAPERVDQVIFGNVLQAGLGQNVARQIAIKAGIPYKVP
GVTINEVCGSGLKSVMLGRQAIQLGEADIVAVGGTENMSQAPLLLNPELAGEEIDPKKLRNSMLIDGLTDVYGEYHMGIT
AENVAEKFSVTREEQDEFAHNSQMKAANAQEKNLFEEEIIPVKLPDGSFFEADETIRANSTLEKLATLKSVFKEGGTVTA
GNASGINDGASAIILMSKEKAVAENIPYIATIKVTSEVGVDPALMGYAPYYAVNEALTKGGYSIDDIDLFHLNEAFASQS
VAVARDLKIPEEKLNIYGGAIALGHPIGASGARIIASLLNELKHENKHIGVASLCVGGGIGIAIILEKA
>Mature_389_residues
MNEVVIIDAVRTPIGKFGGSLKDISAVDLGATALKGVLERANIAPERVDQVIFGNVLQAGLGQNVARQIAIKAGIPYKVP
GVTINEVCGSGLKSVMLGRQAIQLGEADIVAVGGTENMSQAPLLLNPELAGEEIDPKKLRNSMLIDGLTDVYGEYHMGIT
AENVAEKFSVTREEQDEFAHNSQMKAANAQEKNLFEEEIIPVKLPDGSFFEADETIRANSTLEKLATLKSVFKEGGTVTA
GNASGINDGASAIILMSKEKAVAENIPYIATIKVTSEVGVDPALMGYAPYYAVNEALTKGGYSIDDIDLFHLNEAFASQS
VAVARDLKIPEEKLNIYGGAIALGHPIGASGARIIASLLNELKHENKHIGVASLCVGGGIGIAIILEKA

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=48.469387755102, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=43.7017994858612, Blast_Score=305, Evalue=4e-83,
Organism=Homo sapiens, GI4557237, Length=394, Percent_Identity=41.8781725888325, Blast_Score=288, Evalue=7e-78,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=40.253164556962, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=34.9056603773585, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI194353979, Length=395, Percent_Identity=31.8987341772152, Blast_Score=165, Evalue=6e-41,
Organism=Escherichia coli, GI87082165, Length=391, Percent_Identity=48.3375959079284, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=51.7857142857143, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=40.75, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI48994986, Length=400, Percent_Identity=39.5, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1788683, Length=426, Percent_Identity=29.5774647887324, Blast_Score=178, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI133906874, Length=389, Percent_Identity=46.0154241645244, Blast_Score=329, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17535921, Length=393, Percent_Identity=39.1857506361323, Blast_Score=251, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI25147385, Length=390, Percent_Identity=36.4102564102564, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17551802, Length=426, Percent_Identity=33.5680751173709, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17535917, Length=401, Percent_Identity=29.426433915212, Blast_Score=160, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6325229, Length=395, Percent_Identity=42.7848101265823, Blast_Score=299, Evalue=5e-82,
Organism=Saccharomyces cerevisiae, GI6322031, Length=395, Percent_Identity=40.5063291139241, Blast_Score=260, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=48.0818414322251, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=42.6020408163265, Blast_Score=298, Evalue=3e-81,
Organism=Drosophila melanogaster, GI17648125, Length=389, Percent_Identity=42.9305912596401, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=31.3679245283019, Blast_Score=188, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41034; Mature: 41034

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVVIIDAVRTPIGKFGGSLKDISAVDLGATALKGVLERANIAPERVDQVIFGNVLQAG
CCCEEEEECHHCCHHHHCCCCCCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LGQNVARQIAIKAGIPYKVPGVTINEVCGSGLKSVMLGRQAIQLGEADIVAVGGTENMSQ
CCHHHHHHHHHCCCCCEECCCCCHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
APLLLNPELAGEEIDPKKLRNSMLIDGLTDVYGEYHMGITAENVAEKFSVTREEQDEFAH
CCEEECCCCCCCCCCHHHHHHHHHHCCHHHHCCCHHCCCCHHHHHHHHHCCHHHHHHHHH
NSQMKAANAQEKNLFEEEIIPVKLPDGSFFEADETIRANSTLEKLATLKSVFKEGGTVTA
CCCCCCCCCHHHCCHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
GNASGINDGASAIILMSKEKAVAENIPYIATIKVTSEVGVDPALMGYAPYYAVNEALTKG
CCCCCCCCCCCEEEEEECCHHHHHCCCEEEEEEEECCCCCCHHHHCCCCHHHHHHHHHCC
GYSIDDIDLFHLNEAFASQSVAVARDLKIPEEKLNIYGGAIALGHPIGASGARIIASLLN
CCCCCCEEEEECCHHHHCCCHHHHHHCCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
ELKHENKHIGVASLCVGGGIGIAIILEKA
HHHHCCCCCCHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure
MNEVVIIDAVRTPIGKFGGSLKDISAVDLGATALKGVLERANIAPERVDQVIFGNVLQAG
CCCEEEEECHHCCHHHHCCCCCCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LGQNVARQIAIKAGIPYKVPGVTINEVCGSGLKSVMLGRQAIQLGEADIVAVGGTENMSQ
CCHHHHHHHHHCCCCCEECCCCCHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
APLLLNPELAGEEIDPKKLRNSMLIDGLTDVYGEYHMGITAENVAEKFSVTREEQDEFAH
CCEEECCCCCCCCCCHHHHHHHHHHCCHHHHCCCHHCCCCHHHHHHHHHCCHHHHHHHHH
NSQMKAANAQEKNLFEEEIIPVKLPDGSFFEADETIRANSTLEKLATLKSVFKEGGTVTA
CCCCCCCCCHHHCCHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
GNASGINDGASAIILMSKEKAVAENIPYIATIKVTSEVGVDPALMGYAPYYAVNEALTKG
CCCCCCCCCCCEEEEEECCHHHHHCCCEEEEEEEECCCCCCHHHHCCCCHHHHHHHHHCC
GYSIDDIDLFHLNEAFASQSVAVARDLKIPEEKLNIYGGAIALGHPIGASGARIIASLLN
CCCCCCEEEEECCHHHHCCCHHHHHHCCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
ELKHENKHIGVASLCVGGGIGIAIILEKA
HHHHCCCCCCHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]