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The map label for this gene is lisK

Identifier: 226223979

GI number: 226223979

Start: 1386933

End: 1388384

Strand: Direct

Name: lisK

Synonym: Lm4b_01387

Alternate gene names: 226223979

Gene position: 1386933-1388384 (Clockwise)

Preceding gene: 226223978

Following gene: 226223983

Centisome position: 47.62

GC content: 36.36

Gene sequence:

>1452_bases
ATGACGACAAGCCCATTTTCCTTAAAAAGTCGTTCTTTGAAATTCAAATGGACTTTTGGAGCTAGTGCAGCTATTTTTCT
AACTTTTTTCTTATTTTCCTATGCCATTTACCAAGGAATTGGGCAAATGTTGTTAAATGAAGAAGAACCAGAAGTAAAAG
AACTGCTTCTAGCAACAACGAGCACTTTAACGAATCAAGATTTGACCGATAATGAGGAAATTAAGTATTTATTTAATAAC
GATAAAACGGTGAATCGAAAATTACAAGATCAAGTAATTAATCTGTATGATAAAGACGGCCATTTTATTAATAAGTATTA
TTTTTCAAGAAGTCAAGATATTACTAGCATTGATTTTTCGCAGTATTTTGTTAGTGGTACGGACAAGTTTATTATGAATA
AACCAACGATTGACGGACAGAAGATGATGACGGCGCAAATGCCCATTGTAGCGGACGACAATACGACCGTGATTGGTTAC
GCGCAGGTGGTAAATCCGCTCACTTCCTATAATCGGATGATGGACCGGCTTCTCGTTACGATGATTTTACTTGGGGCAGT
GGCGCTCTTTATTAGTGGAATGCTCGGCTACTTGCTAGCACAAAATTTCTTAAACCCACTGACTCGTCTAGCTCGAACAA
TGAATGATATTCGAAAAAATGGTTTCCAAAAACGAATCGAAACGAAAACCAATTCCCGCGATGAAATTGGCGAATTGACC
GTTGTTTTTAATGATATGATGACACGAATTGAAACTAGTTTTGAACAGCAAAAGCAATTCGTAGAGGATGCTTCTCATGA
ATTACGTACCCCAGTGCAAATTATGGAAGGCCATTTAAAATTACTCACTCGTTGGGGAAAAGATGATCCGGCTGTACTTG
ATGAATCATTAAACGCTTCGTTAACGGAATTAGAACGTATGAAAAAATTAGTACAAGAAATGCTTGATTTATCAAGAGCG
GAACAAATTTCACAAACAAAAGAATTACAAATTACCGATGTAAATGCAACAGTAGAACAAGTAAGACGTAATTTTGAAGT
GATGTATGAAAATTTCACGTTTACTTTAAAAGAAGATGATACTGATTTACGAGCACTTATTCAACATAATCATTTAGAGC
AGATTTTAATTATTATTATGGATAATGCCGTGAAGTATTCAGGTGACGGCACCGAAGTGGATATGCATGTCTATAAAGAA
CAAAAGCAAATCCATATTGATGTGCGCGATTACGGGGAAGGCATCTCACAAGAAGAGATTGATAAAATATTCAATCGCTT
CTACCGTGTAGATAAAGCCAGAAGCCGCGAAAAAGGTGGTAATGGCCTCGGACTTGCGATTGCTAAACAATTAGTCGAAG
GATATTTAGGAACGATTAATGCGGTTAGTGAGCCAGATAAAGGTACAACGATAAAAATTACACTTCCTTACATTGAACCA
AAATCCAAATAA

Upstream 100 bases:

>100_bases
TAGTGGATGTGTACGTTCGTTACTTACGAAATAAAATTGATCATCCTGACGAAGAAAGTTATATCCAAACAGTTCGCGGG
ACAGGGTATGTGATGCGTAC

Downstream 100 bases:

>100_bases
AAAAACCGCAACCTTATGACGAGGTTGCGGTTTTTTTTATTTTTCTTTCTTTTGTTCTTCCACGATTTGCTCGAATTCTT
TTTCTTCTTGCTCCATTACT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTTSPFSLKSRSLKFKWTFGASAAIFLTFFLFSYAIYQGIGQMLLNEEEPEVKELLLATTSTLTNQDLTDNEEIKYLFNN
DKTVNRKLQDQVINLYDKDGHFINKYYFSRSQDITSIDFSQYFVSGTDKFIMNKPTIDGQKMMTAQMPIVADDNTTVIGY
AQVVNPLTSYNRMMDRLLVTMILLGAVALFISGMLGYLLAQNFLNPLTRLARTMNDIRKNGFQKRIETKTNSRDEIGELT
VVFNDMMTRIETSFEQQKQFVEDASHELRTPVQIMEGHLKLLTRWGKDDPAVLDESLNASLTELERMKKLVQEMLDLSRA
EQISQTKELQITDVNATVEQVRRNFEVMYENFTFTLKEDDTDLRALIQHNHLEQILIIIMDNAVKYSGDGTEVDMHVYKE
QKQIHIDVRDYGEGISQEEIDKIFNRFYRVDKARSREKGGNGLGLAIAKQLVEGYLGTINAVSEPDKGTTIKITLPYIEP
KSK

Sequences:

>Translated_483_residues
MTTSPFSLKSRSLKFKWTFGASAAIFLTFFLFSYAIYQGIGQMLLNEEEPEVKELLLATTSTLTNQDLTDNEEIKYLFNN
DKTVNRKLQDQVINLYDKDGHFINKYYFSRSQDITSIDFSQYFVSGTDKFIMNKPTIDGQKMMTAQMPIVADDNTTVIGY
AQVVNPLTSYNRMMDRLLVTMILLGAVALFISGMLGYLLAQNFLNPLTRLARTMNDIRKNGFQKRIETKTNSRDEIGELT
VVFNDMMTRIETSFEQQKQFVEDASHELRTPVQIMEGHLKLLTRWGKDDPAVLDESLNASLTELERMKKLVQEMLDLSRA
EQISQTKELQITDVNATVEQVRRNFEVMYENFTFTLKEDDTDLRALIQHNHLEQILIIIMDNAVKYSGDGTEVDMHVYKE
QKQIHIDVRDYGEGISQEEIDKIFNRFYRVDKARSREKGGNGLGLAIAKQLVEGYLGTINAVSEPDKGTTIKITLPYIEP
KSK
>Mature_482_residues
TTSPFSLKSRSLKFKWTFGASAAIFLTFFLFSYAIYQGIGQMLLNEEEPEVKELLLATTSTLTNQDLTDNEEIKYLFNND
KTVNRKLQDQVINLYDKDGHFINKYYFSRSQDITSIDFSQYFVSGTDKFIMNKPTIDGQKMMTAQMPIVADDNTTVIGYA
QVVNPLTSYNRMMDRLLVTMILLGAVALFISGMLGYLLAQNFLNPLTRLARTMNDIRKNGFQKRIETKTNSRDEIGELTV
VFNDMMTRIETSFEQQKQFVEDASHELRTPVQIMEGHLKLLTRWGKDDPAVLDESLNASLTELERMKKLVQEMLDLSRAE
QISQTKELQITDVNATVEQVRRNFEVMYENFTFTLKEDDTDLRALIQHNHLEQILIIIMDNAVKYSGDGTEVDMHVYKEQ
KQIHIDVRDYGEGISQEEIDKIFNRFYRVDKARSREKGGNGLGLAIAKQLVEGYLGTINAVSEPDKGTTIKITLPYIEPK
SK

Specific function: Member of the two-component regulatory system ArlS/ArlR. ArlS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to ArlR [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=27.4021352313167, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786600, Length=226, Percent_Identity=31.858407079646, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786783, Length=348, Percent_Identity=26.1494252873563, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790346, Length=281, Percent_Identity=29.1814946619217, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788713, Length=282, Percent_Identity=26.9503546099291, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790861, Length=370, Percent_Identity=24.8648648648649, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI145693157, Length=241, Percent_Identity=26.9709543568465, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788279, Length=310, Percent_Identity=24.5161290322581, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1786912, Length=248, Percent_Identity=28.2258064516129, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787894, Length=238, Percent_Identity=25.6302521008403, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788549, Length=272, Percent_Identity=26.1029411764706, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=27.5423728813559, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789403, Length=278, Percent_Identity=24.1007194244604, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87082128, Length=306, Percent_Identity=25.1633986928105, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790551, Length=298, Percent_Identity=23.8255033557047, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI48994928, Length=242, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=25.2293577981651, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081816, Length=291, Percent_Identity=23.0240549828179, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1789808, Length=300, Percent_Identity=23.3333333333333, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55461; Mature: 55330

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSPFSLKSRSLKFKWTFGASAAIFLTFFLFSYAIYQGIGQMLLNEEEPEVKELLLATT
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
STLTNQDLTDNEEIKYLFNNDKTVNRKLQDQVINLYDKDGHFINKYYFSRSQDITSIDFS
HHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCEEEHH
QYFVSGTDKFIMNKPTIDGQKMMTAQMPIVADDNTTVIGYAQVVNPLTSYNRMMDRLLVT
HHHHCCCCCEEECCCCCCCCHHHEECCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHH
MILLGAVALFISGMLGYLLAQNFLNPLTRLARTMNDIRKNGFQKRIETKTNSRDEIGELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VVFNDMMTRIETSFEQQKQFVEDASHELRTPVQIMEGHLKLLTRWGKDDPAVLDESLNAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCH
LTELERMKKLVQEMLDLSRAEQISQTKELQITDVNATVEQVRRNFEVMYENFTFTLKEDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHHHHHHHHEEEECEEEEEECCC
TDLRALIQHNHLEQILIIIMDNAVKYSGDGTEVDMHVYKEQKQIHIDVRDYGEGISQEEI
HHHHHHHHHCCHHHHHHEEECCCEEECCCCCEEEHHEEECCCEEEEEHHHHCCCCCHHHH
DKIFNRFYRVDKARSREKGGNGLGLAIAKQLVEGYLGTINAVSEPDKGTTIKITLPYIEP
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
KSK
CCC
>Mature Secondary Structure 
TTSPFSLKSRSLKFKWTFGASAAIFLTFFLFSYAIYQGIGQMLLNEEEPEVKELLLATT
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
STLTNQDLTDNEEIKYLFNNDKTVNRKLQDQVINLYDKDGHFINKYYFSRSQDITSIDFS
HHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCEEEHH
QYFVSGTDKFIMNKPTIDGQKMMTAQMPIVADDNTTVIGYAQVVNPLTSYNRMMDRLLVT
HHHHCCCCCEEECCCCCCCCHHHEECCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHH
MILLGAVALFISGMLGYLLAQNFLNPLTRLARTMNDIRKNGFQKRIETKTNSRDEIGELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VVFNDMMTRIETSFEQQKQFVEDASHELRTPVQIMEGHLKLLTRWGKDDPAVLDESLNAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCH
LTELERMKKLVQEMLDLSRAEQISQTKELQITDVNATVEQVRRNFEVMYENFTFTLKEDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHHHHHHHHEEEECEEEEEECCC
TDLRALIQHNHLEQILIIIMDNAVKYSGDGTEVDMHVYKEQKQIHIDVRDYGEGISQEEI
HHHHHHHHHCCHHHHHHEEECCCEEECCCCCEEEHHEEECCCEEEEEHHHHCCCCCHHHH
DKIFNRFYRVDKARSREKGGNGLGLAIAKQLVEGYLGTINAVSEPDKGTTIKITLPYIEP
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
KSK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA