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The map label for this gene is bfmBB [H]

Identifier: 226223975

GI number: 226223975

Start: 1381785

End: 1383035

Strand: Direct

Name: bfmBB [H]

Synonym: Lm4b_01383

Alternate gene names: 226223975

Gene position: 1381785-1383035 (Clockwise)

Preceding gene: 226223974

Following gene: 226223976

Centisome position: 47.44

GC content: 39.73

Gene sequence:

>1251_bases
GTGGCAGTTGAAAAAATCACCATGCCCAAATTAGGGGAAAGTGTTACAGAAGGTACGATTAGTTCATGGTTAGTTAAACC
AGGCGATACCGTCGAAAAATATGATGCTATCGCGGAAGTTTTAACAGATAAAGTAACAGCTGAAATTCCATCATCCTTTA
GTGGCACTATCAAAGAAATTTTAGCAGAGGAAGATGAAACTCTAGAAGTAGGCGAAGTTATTTGTACAATTGAAACAGCA
GATGCAGGAAGTTCGGAACCTGTAGCTGAAGTAGAAGAAACAGAAACAAAAGCGCCAGAAAAACAAGAAACAAAACAAGT
GAAACTAGCAGATGCACCAGCTAGTGGAAGATTTTCTCCAGCTGTACTGCGTATTGCAGGTGAAAACAATATTGATTTAT
CAACTGTAGAAGGCACTGGTAAAGGTGGCCGAATTACAAGAAAAGATTTACTTCAAGTCATTGAAAATGGTCCAGTAGCG
AAACGCGAGGAAATGAAGTCTGCTCCACAAGAAAAAGCAGCAATGCCAACTCCTCCTGTTCGTTCTGCAGCTGGTGATAA
AGAAATACCAATCAATGGCGTAAGAAAAGCCATTGCTAAACATATGAGCGTGAGTAAACAAGAAATTCCGCATGCTTGGA
TGATGGTGGAAGTGGATGCTACTGGTCTTGTTCGCTATCGTAATGCAGTTAAAGACAGCTTTAAAAAAGAAGAAGGTTAT
TCATTAACTTATTTCGCCTTTTTCATCAAAGCCGTTGCACAAGCATTGAAAGAATTCCCGCAACTTAACAGCACATGGGC
AGGCGATAAAATTATCGAGCACGCGAATATCAATATTTCGATTGCGATTGCAGCTGGTGATTTATTGTACGTTCCAGTTA
TTAAAAATGCGGACGAAAAATCTATTAAAGGCATTGCTCGCGAAATTAGCGAACTTGCTGGAAAAGCGCGTAATGGTAAA
CTAAGCCAAGCCGATATGGAAGGTGGGACTTTCACTGTAAATAGTACTGGTTCATTTGGCTCTGTTCAATCAATGGGGAT
TATTAACCACCCTCAGGCCGCTATTCTTCAAGTGGAATCGATTGTTAAACGTCCTGTCATTATTGACGATATGATTGCCG
TGCGAGATATGGTGAACCTATGTCTATCCATCGATCATCGTATTTTAGATGGCTTACTAGCAGGTAAATTCTTACAAGCA
ATTAAAGCCAATGTCGAAAAGATTTCCAAAGAAAATACAGCATTGTATTAA

Upstream 100 bases:

>100_bases
GATGGAAAAACACTTTATGATCAATCCAGATAAAGTGGCAGATGCAATGAAAGAATTAGCGGAATTTTAGACCCGTTTTA
AAATAAAGGAGTGAAGACCT

Downstream 100 bases:

>100_bases
AAATGTTTTAATCTAGGTTTAGCGGGTATTGTTTAGTACATCTAGATCCATACCCCTAAACTCCCTAGATGTCTTGGGTA
GTACTTTCGGGTGCTGCTCT

Product: branched-chain alpha-keto acid dehydrogenase E2 subunit (lipoamide acyltransferase)

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MAVEKITMPKLGESVTEGTISSWLVKPGDTVEKYDAIAEVLTDKVTAEIPSSFSGTIKEILAEEDETLEVGEVICTIETA
DAGSSEPVAEVEETETKAPEKQETKQVKLADAPASGRFSPAVLRIAGENNIDLSTVEGTGKGGRITRKDLLQVIENGPVA
KREEMKSAPQEKAAMPTPPVRSAAGDKEIPINGVRKAIAKHMSVSKQEIPHAWMMVEVDATGLVRYRNAVKDSFKKEEGY
SLTYFAFFIKAVAQALKEFPQLNSTWAGDKIIEHANINISIAIAAGDLLYVPVIKNADEKSIKGIAREISELAGKARNGK
LSQADMEGGTFTVNSTGSFGSVQSMGIINHPQAAILQVESIVKRPVIIDDMIAVRDMVNLCLSIDHRILDGLLAGKFLQA
IKANVEKISKENTALY

Sequences:

>Translated_416_residues
MAVEKITMPKLGESVTEGTISSWLVKPGDTVEKYDAIAEVLTDKVTAEIPSSFSGTIKEILAEEDETLEVGEVICTIETA
DAGSSEPVAEVEETETKAPEKQETKQVKLADAPASGRFSPAVLRIAGENNIDLSTVEGTGKGGRITRKDLLQVIENGPVA
KREEMKSAPQEKAAMPTPPVRSAAGDKEIPINGVRKAIAKHMSVSKQEIPHAWMMVEVDATGLVRYRNAVKDSFKKEEGY
SLTYFAFFIKAVAQALKEFPQLNSTWAGDKIIEHANINISIAIAAGDLLYVPVIKNADEKSIKGIAREISELAGKARNGK
LSQADMEGGTFTVNSTGSFGSVQSMGIINHPQAAILQVESIVKRPVIIDDMIAVRDMVNLCLSIDHRILDGLLAGKFLQA
IKANVEKISKENTALY
>Mature_415_residues
AVEKITMPKLGESVTEGTISSWLVKPGDTVEKYDAIAEVLTDKVTAEIPSSFSGTIKEILAEEDETLEVGEVICTIETAD
AGSSEPVAEVEETETKAPEKQETKQVKLADAPASGRFSPAVLRIAGENNIDLSTVEGTGKGGRITRKDLLQVIENGPVAK
REEMKSAPQEKAAMPTPPVRSAAGDKEIPINGVRKAIAKHMSVSKQEIPHAWMMVEVDATGLVRYRNAVKDSFKKEEGYS
LTYFAFFIKAVAQALKEFPQLNSTWAGDKIIEHANINISIAIAAGDLLYVPVIKNADEKSIKGIAREISELAGKARNGKL
SQADMEGGTFTVNSTGSFGSVQSMGIINHPQAAILQVESIVKRPVIIDDMIAVRDMVNLCLSIDHRILDGLLAGKFLQAI
KANVEKISKENTALY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=418, Percent_Identity=33.4928229665072, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI203098816, Length=447, Percent_Identity=31.5436241610738, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI203098753, Length=447, Percent_Identity=31.5436241610738, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=30.6264501160093, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI19923748, Length=218, Percent_Identity=37.1559633027523, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=35.2564102564103, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1786946, Length=407, Percent_Identity=32.6781326781327, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786305, Length=429, Percent_Identity=28.4382284382284, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=32.3809523809524, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=31.4350797266515, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=410, Percent_Identity=30.2439024390244, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17538894, Length=295, Percent_Identity=29.4915254237288, Blast_Score=128, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=413, Percent_Identity=30.9927360774818, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324258, Length=447, Percent_Identity=27.2930648769575, Blast_Score=136, Evalue=7e-33,
Organism=Drosophila melanogaster, GI18859875, Length=430, Percent_Identity=31.3953488372093, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24645909, Length=221, Percent_Identity=34.841628959276, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI20129315, Length=222, Percent_Identity=32.8828828828829, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24582497, Length=223, Percent_Identity=32.7354260089686, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 44889; Mature: 44758

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEKITMPKLGESVTEGTISSWLVKPGDTVEKYDAIAEVLTDKVTAEIPSSFSGTIKEI
CCCCCCCCCHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LAEEDETLEVGEVICTIETADAGSSEPVAEVEETETKAPEKQETKQVKLADAPASGRFSP
HCCCCCCEEECEEEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHCEEEEECCCCCCCCCC
AVLRIAGENNIDLSTVEGTGKGGRITRKDLLQVIENGPVAKREEMKSAPQEKAAMPTPPV
EEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHCCCCCCCC
RSAAGDKEIPINGVRKAIAKHMSVSKQEIPHAWMMVEVDATGLVRYRNAVKDSFKKEEGY
CCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCC
SLTYFAFFIKAVAQALKEFPQLNSTWAGDKIIEHANINISIAIAAGDLLYVPVIKNADEK
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCEEEEEEEECCEEEEEEECCCCHH
SIKGIAREISELAGKARNGKLSQADMEGGTFTVNSTGSFGSVQSMGIINHPQAAILQVES
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHCCCCCCCCHHHHHHHHH
IVKRPVIIDDMIAVRDMVNLCLSIDHRILDGLLAGKFLQAIKANVEKISKENTALY
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AVEKITMPKLGESVTEGTISSWLVKPGDTVEKYDAIAEVLTDKVTAEIPSSFSGTIKEI
CCCCCCCCHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LAEEDETLEVGEVICTIETADAGSSEPVAEVEETETKAPEKQETKQVKLADAPASGRFSP
HCCCCCCEEECEEEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHCEEEEECCCCCCCCCC
AVLRIAGENNIDLSTVEGTGKGGRITRKDLLQVIENGPVAKREEMKSAPQEKAAMPTPPV
EEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHCCCCCCCC
RSAAGDKEIPINGVRKAIAKHMSVSKQEIPHAWMMVEVDATGLVRYRNAVKDSFKKEEGY
CCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCC
SLTYFAFFIKAVAQALKEFPQLNSTWAGDKIIEHANINISIAIAAGDLLYVPVIKNADEK
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCEEEEEEEECCEEEEEEECCCCHH
SIKGIAREISELAGKARNGKLSQADMEGGTFTVNSTGSFGSVQSMGIINHPQAAILQVES
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHCCCCCCCCHHHHHHHHH
IVKRPVIIDDMIAVRDMVNLCLSIDHRILDGLLAGKFLQAIKANVEKISKENTALY
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]